Juan Pablo Tosar
@jptosar.bsky.social
RNA Biology & extracellular RNA. Group Leader, Functional Genomics Lab at Pasteur Institute of Montevideo / Analytical Biochemistry Unit at Universidad de la República, Uruguay.
With @amyhbuck we wrote this piece on extracellular RNAs that intends not to give answers but to propose questions that can expand the field in new directions.
Hope you like it! We really enjoyed a lot brainstorming it.
journals.asm.org/doi/10.1128/...
Hope you like it! We really enjoyed a lot brainstorming it.
journals.asm.org/doi/10.1128/...
mGem: Guides or triggers? Extracellular RNAs beyond vesicular miRNAs | mBio
Blood and other extracellular biofluids have a high RNase content. Hence, from its
origins, the exRNA field has focused on extracellular vesicles (EVs) as the main carriers
of RNA molecules that move ...
journals.asm.org
September 17, 2025 at 3:38 AM
With @amyhbuck we wrote this piece on extracellular RNAs that intends not to give answers but to propose questions that can expand the field in new directions.
Hope you like it! We really enjoyed a lot brainstorming it.
journals.asm.org/doi/10.1128/...
Hope you like it! We really enjoyed a lot brainstorming it.
journals.asm.org/doi/10.1128/...
July 18, 2025 at 5:08 PM
Reposted by Juan Pablo Tosar
🔥🔥🔥NEW PAPER OUT in @genomebiolevol.bsky.social
with @psarkies.bsky.social and many others who are not here, yet!🔥🔥🔥
academic.oup.com/gbe/advance-...
with @psarkies.bsky.social and many others who are not here, yet!🔥🔥🔥
academic.oup.com/gbe/advance-...
Substantial hierarchical reductions of genetic and morphological traits in the evolution of rotiferan parasites
Abstract. Within the last 800 million years animals evolved a vast range of diversity of species exhibiting an enormous disparity of forms and lifestyles.
academic.oup.com
June 23, 2025 at 1:51 PM
🔥🔥🔥NEW PAPER OUT in @genomebiolevol.bsky.social
with @psarkies.bsky.social and many others who are not here, yet!🔥🔥🔥
academic.oup.com/gbe/advance-...
with @psarkies.bsky.social and many others who are not here, yet!🔥🔥🔥
academic.oup.com/gbe/advance-...
Check out our paper about naked extracellular RNAs uptake, TLR signaling, endosomal escape, and the role of RNases in regulating this process.
www.cell.com/cell-genomic...
www.cell.com/cell-genomic...
Ribonuclease activity undermines immune sensing of naked extracellular RNA
Castellano et al. show that when extracellular RNases are inhibited or absent, naked
extracellular RNA is internalized spontaneously by cells, inducing inflammation mediated
by endosomal TLRs or cytos...
www.cell.com
May 7, 2025 at 11:44 AM
Check out our paper about naked extracellular RNAs uptake, TLR signaling, endosomal escape, and the role of RNases in regulating this process.
www.cell.com/cell-genomic...
www.cell.com/cell-genomic...
New preprint: we isolated an heterogeneous population comprising both large and small #ExtracellularVesicles. Using a modified immuno-gold protocol, we show that, at least in U-2 OS EVs, CD9 and CD81 are enriched in EVs < 100 nm.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
March 18, 2025 at 11:56 AM
New preprint: we isolated an heterogeneous population comprising both large and small #ExtracellularVesicles. Using a modified immuno-gold protocol, we show that, at least in U-2 OS EVs, CD9 and CD81 are enriched in EVs < 100 nm.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Juan Pablo Tosar
There are concerns that Bluesky's impact has faded since the initial hype. This is false.
That impression results from reading headlines about social media instead of the hard data.
The five HIGHEST points of Bluesky posts mentioning research all occur in the last 2 weeks.
That impression results from reading headlines about social media instead of the hard data.
The five HIGHEST points of Bluesky posts mentioning research all occur in the last 2 weeks.
March 10, 2025 at 4:21 PM
There are concerns that Bluesky's impact has faded since the initial hype. This is false.
That impression results from reading headlines about social media instead of the hard data.
The five HIGHEST points of Bluesky posts mentioning research all occur in the last 2 weeks.
That impression results from reading headlines about social media instead of the hard data.
The five HIGHEST points of Bluesky posts mentioning research all occur in the last 2 weeks.
Not all tRNA fragments (tDRs) are created equal. Here we share methods to differentiate:
1) real tDRs and those produced by dissociation of nicked tRNAs, 2) nicked tRNAs / tDRs in extracellular vesicles vs outside.
1) www.sciencedirect.com/science/arti...
2) www.sciencedirect.com/science/arti...
1) real tDRs and those produced by dissociation of nicked tRNAs, 2) nicked tRNAs / tDRs in extracellular vesicles vs outside.
1) www.sciencedirect.com/science/arti...
2) www.sciencedirect.com/science/arti...
February 1, 2025 at 4:00 PM
Not all tRNA fragments (tDRs) are created equal. Here we share methods to differentiate:
1) real tDRs and those produced by dissociation of nicked tRNAs, 2) nicked tRNAs / tDRs in extracellular vesicles vs outside.
1) www.sciencedirect.com/science/arti...
2) www.sciencedirect.com/science/arti...
1) real tDRs and those produced by dissociation of nicked tRNAs, 2) nicked tRNAs / tDRs in extracellular vesicles vs outside.
1) www.sciencedirect.com/science/arti...
2) www.sciencedirect.com/science/arti...
Reposted by Juan Pablo Tosar
Here's a clever new study looking at where mRNA vaccine lipid nanoparticles (mRNA-LNPs) go after vaccination (using relevant doses of vaccine) in non-human primates (and mice): www.cell.com/molecular-th...
Brief thread🧵
Brief thread🧵
January 12, 2025 at 3:17 PM
Here's a clever new study looking at where mRNA vaccine lipid nanoparticles (mRNA-LNPs) go after vaccination (using relevant doses of vaccine) in non-human primates (and mice): www.cell.com/molecular-th...
Brief thread🧵
Brief thread🧵
GRC on RNA Nanotechnology came to an end yesterday. Thanks to the organizers for inviting me to present our work on the uptake of naked exRNAs, and to serve as discussion leader of the RNA Delivery session. What a fantastic and enjoyable conference!
www.grc.org/rna-nanotech...
www.grc.org/rna-nanotech...
January 11, 2025 at 10:48 AM
GRC on RNA Nanotechnology came to an end yesterday. Thanks to the organizers for inviting me to present our work on the uptake of naked exRNAs, and to serve as discussion leader of the RNA Delivery session. What a fantastic and enjoyable conference!
www.grc.org/rna-nanotech...
www.grc.org/rna-nanotech...
Reposted by Juan Pablo Tosar
Very happy to start the new year with publication of our new work on leaf surface RNA. Plant leaves secrete diverse RNAs that are surprisingly stable on their surfaces. Next question to answer: What do they do? www.pnas.org/doi/epub/10....
January 3, 2025 at 8:21 PM
Very happy to start the new year with publication of our new work on leaf surface RNA. Plant leaves secrete diverse RNAs that are surprisingly stable on their surfaces. Next question to answer: What do they do? www.pnas.org/doi/epub/10....
Reposted by Juan Pablo Tosar
"Here, we show that this Ab appears to be unreliable due to providing false-positive signals at the expected molecular weight of Bax in immunoblotting experiments and likewise provides false-positive signals in immunofluorescence-based detection of Bax expression"
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Why Bax detection in >1400 publications might be flawed - Cell Death & Disease
Cell Death & Disease - Why Bax detection in >1400 publications might be flawed
www.nature.com
December 25, 2024 at 7:20 PM
"Here, we show that this Ab appears to be unreliable due to providing false-positive signals at the expected molecular weight of Bax in immunoblotting experiments and likewise provides false-positive signals in immunofluorescence-based detection of Bax expression"
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Juan Pablo Tosar
Some of you probably do not realize how difficult it is to get a BIG international grant when located in LMIC (especially in Argentina). I am so proud of you @pstrobl.bsky.social. So well deserved. I am so happy to be part. You are the best!! @wellcometrust.bsky.social @unsamoficial.bsky.social
Thrilled to share that our project will be granted by @wellcometrust.bsky.social for the following 8 years at @IntechChascomus @unsamoficial.bsky.social I would like to thanks the collaborator of the project @ndemiguel.bsky.social and @eliasbarrigalab.bsky.social for all the support.
December 18, 2024 at 3:36 PM
Some of you probably do not realize how difficult it is to get a BIG international grant when located in LMIC (especially in Argentina). I am so proud of you @pstrobl.bsky.social. So well deserved. I am so happy to be part. You are the best!! @wellcometrust.bsky.social @unsamoficial.bsky.social
Reposted by Juan Pablo Tosar
But please dont share this as a mantra for success. If your success comes at the direct expense of your colleagues because they are doing all the work everyone is supposed to share in, it does not make you ‘successful’ it just makes you a horrible colleague.
Also see peer review
Rant over. For now
Also see peer review
Rant over. For now
December 9, 2024 at 3:26 AM
But please dont share this as a mantra for success. If your success comes at the direct expense of your colleagues because they are doing all the work everyone is supposed to share in, it does not make you ‘successful’ it just makes you a horrible colleague.
Also see peer review
Rant over. For now
Also see peer review
Rant over. For now
Reposted by Juan Pablo Tosar
This will be the next level of scientific devaluation: science to proof a political standpoint, rather than policy following scientific proof.
Trump says RFK Jr will “investigate” the link between vaccines and autism as “somebody needs to find out.”
We’ve ALREADY FOUND OUT
- >600,000 kids in Denmark
- >90,000 in the US
- >1.2m kids worldwide
NO LINK.
How many more of these does he want us to do?
We’ve ALREADY FOUND OUT
- >600,000 kids in Denmark
- >90,000 in the US
- >1.2m kids worldwide
NO LINK.
How many more of these does he want us to do?
December 9, 2024 at 12:53 PM
This will be the next level of scientific devaluation: science to proof a political standpoint, rather than policy following scientific proof.
Reposted by Juan Pablo Tosar
MirGeneDB 3.0 is published.
Great teamwork and substantial improvement of database, Taxon sampling and we took over nomenclature of novel genes and families, too
academic.oup.com/nar/advance-...
Great teamwork and substantial improvement of database, Taxon sampling and we took over nomenclature of novel genes and families, too
academic.oup.com/nar/advance-...
MirGeneDB 3.0: improved taxonomic sampling, uniform nomenclature of novel conserved microRNA families and updated covariance models
Abstract. We present a major update of MirGeneDB (3.0), the manually curated animal microRNA gene database. Beyond moving to a new server and the creation
academic.oup.com
November 30, 2024 at 11:46 AM
MirGeneDB 3.0 is published.
Great teamwork and substantial improvement of database, Taxon sampling and we took over nomenclature of novel genes and families, too
academic.oup.com/nar/advance-...
Great teamwork and substantial improvement of database, Taxon sampling and we took over nomenclature of novel genes and families, too
academic.oup.com/nar/advance-...
Reposted by Juan Pablo Tosar
First post, last paper out www.frontiersin.org/journals/tub... @carlos-robello.bsky.social
Frontiers | Serum-derived host miR-423-5p and miR-378a-3p as molecular markers for severe tuberculosis: a promising prognostic tool for survival
Tuberculosis (TB) remains a leading cause of infectious disease-related mortality. Annually, 10 million people contract TB, resulting in 1.5 million deaths, ...
www.frontiersin.org
November 29, 2024 at 9:48 PM
First post, last paper out www.frontiersin.org/journals/tub... @carlos-robello.bsky.social
Reposted by Juan Pablo Tosar
The conserved landscape of RNA modifications and transcript diversity across mammalian evolution https://www.biorxiv.org/content/10.1101/2024.11.24.624934v1
The conserved landscape of RNA modifications and transcript diversity across mammalian evolution https://www.biorxiv.org/content/10.1101/2024.11.24.624934v1
Gene expression programs underpin the development of shared phenotypes, yet the importance of transc
www.biorxiv.org
November 25, 2024 at 10:21 PM
Reposted by Juan Pablo Tosar
The conserved landscape of RNA modifications and transcript diversity across mammalian evolution https://www.biorxiv.org/content/10.1101/2024.11.24.624934v1
The conserved landscape of RNA modifications and transcript diversity across mammalian evolution https://www.biorxiv.org/content/10.1101/2024.11.24.624934v1
Gene expression programs underpin the development of shared phenotypes, yet the importance of transc
www.biorxiv.org
November 25, 2024 at 2:33 AM
The conserved landscape of RNA modifications and transcript diversity across mammalian evolution https://www.biorxiv.org/content/10.1101/2024.11.24.624934v1
Reposted by Juan Pablo Tosar
📢SAVE THE DATE📢
The 4th Molecular Biosystems Conference is set!
Plan to join us in southern Chile for insightful discussions on gene regulation & functional genomics #mbiosys25 🧬🖥️
Stay tuned for more details!
🗓️Sep 29-Oct 3, 2025
📍Puerto Varas, Chile
🌐http://molbiosystems.com
The 4th Molecular Biosystems Conference is set!
Plan to join us in southern Chile for insightful discussions on gene regulation & functional genomics #mbiosys25 🧬🖥️
Stay tuned for more details!
🗓️Sep 29-Oct 3, 2025
📍Puerto Varas, Chile
🌐http://molbiosystems.com
November 18, 2024 at 2:49 PM
📢SAVE THE DATE📢
The 4th Molecular Biosystems Conference is set!
Plan to join us in southern Chile for insightful discussions on gene regulation & functional genomics #mbiosys25 🧬🖥️
Stay tuned for more details!
🗓️Sep 29-Oct 3, 2025
📍Puerto Varas, Chile
🌐http://molbiosystems.com
The 4th Molecular Biosystems Conference is set!
Plan to join us in southern Chile for insightful discussions on gene regulation & functional genomics #mbiosys25 🧬🖥️
Stay tuned for more details!
🗓️Sep 29-Oct 3, 2025
📍Puerto Varas, Chile
🌐http://molbiosystems.com
Reposted by Juan Pablo Tosar
