New preprint: we isolated an heterogeneous population comprising both large and small #ExtracellularVesicles. Using a modified immuno-gold protocol, we show that, at least in U-2 OS EVs, CD9 and CD81 are enriched in EVs < 100 nm.

www.biorxiv.org/content/10.1...

Not all tRNA fragments (tDRs) are created equal. Here we share methods to differentiate:

1) real tDRs and those produced by dissociation of nicked tRNAs, 2) nicked tRNAs / tDRs in extracellular vesicles vs outside.

1) www.sciencedirect.com/science/arti...

2) www.sciencedirect.com/science/arti...

GRC on RNA Nanotechnology came to an end yesterday. Thanks to the organizers for inviting me to present our work on the uptake of naked exRNAs, and to serve as discussion leader of the RNA Delivery session. What a fantastic and enjoyable conference!
www.grc.org/rna-nanotech...

As a relatively new field, it’s following this path. With the exception that, depending who you talk to, we might still be in the peak of inflated expectations, the trough of disillusionment or the slope of enlightenment. There are lots of artifacts going on, but probably also important biology