DNA damage can lead to cell death. Here, the authors show that a simple cut on either strand of DNA can inactivate bacterial chromosome replication. Surprisingly, only a core set of recombination prot...

Postdoctoral Researcher in discovering the benefits of plant growth-promoting bacteriophages

Topics The following will be covered extensively during lectures, exercises, and project work: Image denoising and restoration (fully supervised and self-supervised) Image translation (e.g.,

Industry opportunities

Ribosomes translate the genetic code in mRNA to synthesize proteins in all living organisms. Decoding of mRNA occurs in the small subunit of the ribosome and is mediated by tRNA anticodons. Regions ne...

Secondary metabolites are a major source of natural products with industrially relevant bioactivities. Lysate-based cell-free expression (CFE) is an emerging platform for accelerating the discovery and engineering of these natural products. While Escherichia coli cell extracts are widely used for CFE, Streptomyces extracts are likely to offer a more biochemically compatible environment for their expression. However, current Streptomyces-based CFE systems remain underdeveloped, with protocols that are either strain-specific or not readily scalable. To address these limitations and enable broader access to cell-free natural product biosynthesis, we present a generalizable and simple set of reaction conditions that support high-yield protein expression (180–230 μg/mL) in lysates derived from Streptomyces venezuelae NRRL B-65422 and Streptomyces lividans TK24. Like E. coli-based systems, these extracts enable iterative and pathway-level biosynthesis, as demonstrated by the production of the polyketide flaviolin and the cyclic dipeptide albonoursin. Notably, the S. lividans lysate outperforms the E. coli systems by also supporting the expression and catalytic activity of a (∼250 kDa) type I polyketide synthase (T1PKS), producing its corresponding ethyl ketone product, 2-methyl-3-pentanone, without the need for precursor or post-translational modification supplements. To our knowledge, this represents the first demonstration coupling both expression and catalysis of a megasynthase in a Streptomyces-based system, and of a T1PKS in any bacterial extract. By addressing key challenges in the generalizability and scalability of prior Streptomyces CFE, we establish a protocol that enables parallelized evaluation of diverse lysate systems and provides a foundation for high-throughput T1PKS engineering in vitro.

The Center for Molecular Biology of Heidelberg University (ZMBH) and the Faculty of Biosciences invite applications for a   Tenure Track Professors...

Applications are invited for a professorial appointment to be based in the Manchester Institute of Biotechnology (MIB) at the University of Manchester (UoM). The successful candidate will join a dynamic research environment at an exciting time and will help shape the institute research priorities and national/international profile. We are open to high quality applications from across the broad engineering biology and biotechnology landscape. Specific areas of interest include but are not limited to chemical biology of disease related proteins, natural product biosynthesis and enzyme discovery; structural biology and mechanistic enzymology; and microbial engineering.

All Job Postings will close at 12:01 a.m. CT on the specified Closing Date (if designated). Working Title: Microbiologist (AA27034) Institution: Minnesota State University, Mankato Classification Titl...

Discover Research Associate in Bacterial Transport Mechanisms jobs and more in higher education on jobs.ac.uk. Apply for further details on the top job board.

PhD Project - PhD Studentship: Understanding How Centromere Replication Fails in Cancer Imperial College London & The Institute of Cancer Research (CRUK Convergence Science Centre) at Institute of...

PhD in Microbial Genomics, exciting new Doctoral Training Programme in Microbial Genomics for Health Protection in collaboration with the UK Health Security Agency (UKHSA) launches