Mirian Krystel De Siqueira
@desiqueiramk.bsky.social
Postdoctoral Fellow @Stanford studying #ribosome mediated control of #lipid metabolism | @UCLA @nih @unicamp alumna | @Lindau Nobel Young Scientist 2023
Pinned
PPARγ-dependent remodeling of translational machinery in adipose progenitors is impaired in obesity
De Siqueira et al. explore the effects of rosiglitazone on adipose stem cell plasticity,
showing that PPARγ stimulates expression of ribosomal factors in adipocyte precursors.
This leads to translatio...
www.cell.com
Excited to share our latest work in #CellReports highlighting a PPARγ dependent remodeling of translation machinery in adipose progenitors. Amazing collab between our lab Claudio Villanueva, Xia Yang, and @laurahulea.bsky.social !
#obesity #metabolism #translation
www.cell.com/cell-reports...
#obesity #metabolism #translation
www.cell.com/cell-reports...
Reposted by Mirian Krystel De Siqueira
Ribosomal RNA (rRNA) genes are found in hundreds of copies in the human genome.
Do sequence variations in these paralogs change the ribosome function? Yes!
I am excited to share our new preprint @mbarnalab @jkpritch in collaboration with Calico:
www.medrxiv.org/content/10.1...
1/8
Do sequence variations in these paralogs change the ribosome function? Yes!
I am excited to share our new preprint @mbarnalab @jkpritch in collaboration with Calico:
www.medrxiv.org/content/10.1...
1/8
November 19, 2025 at 11:00 PM
Ribosomal RNA (rRNA) genes are found in hundreds of copies in the human genome.
Do sequence variations in these paralogs change the ribosome function? Yes!
I am excited to share our new preprint @mbarnalab @jkpritch in collaboration with Calico:
www.medrxiv.org/content/10.1...
1/8
Do sequence variations in these paralogs change the ribosome function? Yes!
I am excited to share our new preprint @mbarnalab @jkpritch in collaboration with Calico:
www.medrxiv.org/content/10.1...
1/8
Reposted by Mirian Krystel De Siqueira
ICYMI:
Dietary control of peripheral adipose storage capacity through membrane lipid remodelling
Nature Metabolism, Published online: 27 June 2025; doi:10.1038/s42255-025-01320-yDietary n-6 PUFAs enhance adipose tissue expandability through the PPARγ–LPCAT3 membrane remodelling axis.
bit.ly
July 5, 2025 at 12:19 AM
ICYMI:
Reposted by Mirian Krystel De Siqueira
Excited to share Chapter #1 of our LPCAT3 journey in adipose biology and membrane dynamics—born of a rewarding, years-long, espresso-fueled partnership with Prof. Yuta Shimanaka @shimachu64.bsky.social. www.nature.com/articles/s42...
Dietary control of peripheral adipose storage capacity through membrane lipid remodelling - Nature Metabolism
Dietary n-6 PUFAs enhance adipose tissue expandability through the PPARγ–LPCAT3 membrane remodelling axis.
www.nature.com
July 2, 2025 at 9:22 AM
Excited to share Chapter #1 of our LPCAT3 journey in adipose biology and membrane dynamics—born of a rewarding, years-long, espresso-fueled partnership with Prof. Yuta Shimanaka @shimachu64.bsky.social. www.nature.com/articles/s42...
Congratulations, Marc and Yuta!
www.nature.com/articles/s42...
www.nature.com/articles/s42...
Dietary control of peripheral adipose storage capacity through membrane lipid remodelling - Nature Metabolism
Dietary n-6 PUFAs enhance adipose tissue expandability through the PPARγ–LPCAT3 membrane remodelling axis.
www.nature.com
June 27, 2025 at 4:36 PM
Congratulations, Marc and Yuta!
www.nature.com/articles/s42...
www.nature.com/articles/s42...
Check out the preprint of these two amazing scientists, fat-biologists, and superstar former postdocs Yuta and Marc (BlueSky-less users)!
Super excited to see this story out.
www.biorxiv.org/content/10.1...
Super excited to see this story out.
www.biorxiv.org/content/10.1...
A cold-inducible phospholipid—protein interaction in brown fat mitochondria optimizes thermogenic capacity
Cold stress elicits dynamic remodeling of the mitochondrial lipidome in brown adipose tissue (BAT), marked by an increase in arachidonoyl–phosphatidylethanolamine (AA–PE). However, the function of mem...
www.biorxiv.org
May 19, 2025 at 4:40 PM
Check out the preprint of these two amazing scientists, fat-biologists, and superstar former postdocs Yuta and Marc (BlueSky-less users)!
Super excited to see this story out.
www.biorxiv.org/content/10.1...
Super excited to see this story out.
www.biorxiv.org/content/10.1...
Reposted by Mirian Krystel De Siqueira
Protocol to perform polysome profiling in primary differentiating murine adipocytes #protocol #starprotocols #cellpress
Protocol to perform polysome profiling in primary differentiating murine adipocytes
Here, we present a protocol for polysome profiling in differentiating adipocytes from the mouse stromal vascular fraction. We describe steps for lysate preparation, ultracentrifugation through sucrose gradients, mRNA isolation, RNA sequencing, and motif enrichment. This protocol enables the analysis of actively translating mRNAs and translational gene regulation by isolating polysome-bound mRNA, revealing insights into protein synthesis during adipogenesis or stimuli responses. Applications incl...
dlvr.it
May 1, 2025 at 7:26 PM
Protocol to perform polysome profiling in primary differentiating murine adipocytes #protocol #starprotocols #cellpress
New protocol drop! 🧪 Check out our polysome profiling in primary differentiating murine adipocytes 🐭🧫 — a step-by-step guide to studying translational control during adipogenesis. 👉 star-protocols.cell.com/protocols/4182
@laurahulea.bsky.social @claudiovillanueva.bsky.social
@laurahulea.bsky.social @claudiovillanueva.bsky.social
May 5, 2025 at 6:25 PM
New protocol drop! 🧪 Check out our polysome profiling in primary differentiating murine adipocytes 🐭🧫 — a step-by-step guide to studying translational control during adipogenesis. 👉 star-protocols.cell.com/protocols/4182
@laurahulea.bsky.social @claudiovillanueva.bsky.social
@laurahulea.bsky.social @claudiovillanueva.bsky.social
Reposted by Mirian Krystel De Siqueira
Grateful to @berkeleyMCB for highlighting our lab and our recent discoveries! It was fun to discuss some of the surprises during our research on lipid homeostasis, lipid droplets, & ferroptosis—driven by exceptional UC Berkeley students & postdocs. mcb.berkeley.edu/news-and-eve...
Fall 2024 | Research Spotlight: James Olzmann
Faculty Research Spotlight James Olzmann: Big Questions and Aha! Moments By Kirsten Mickelwait James Olzmann James Olzmann’s lab has certainly had its share of “aha!” moments. These come not just from...
mcb.berkeley.edu
November 27, 2024 at 12:27 AM
Grateful to @berkeleyMCB for highlighting our lab and our recent discoveries! It was fun to discuss some of the surprises during our research on lipid homeostasis, lipid droplets, & ferroptosis—driven by exceptional UC Berkeley students & postdocs. mcb.berkeley.edu/news-and-eve...
Fat Cells in Full Bloom!
Check out our cover on #CellReports
1/5 In a vibrant homage to Van Gogh's iconic Sunflowers, we introduce the Rosiflowers - a wonder born from the fertile depths of the adipose stromal vascular fraction.
Check out our cover on #CellReports
1/5 In a vibrant homage to Van Gogh's iconic Sunflowers, we introduce the Rosiflowers - a wonder born from the fertile depths of the adipose stromal vascular fraction.
November 26, 2024 at 6:04 PM
Fat Cells in Full Bloom!
Check out our cover on #CellReports
1/5 In a vibrant homage to Van Gogh's iconic Sunflowers, we introduce the Rosiflowers - a wonder born from the fertile depths of the adipose stromal vascular fraction.
Check out our cover on #CellReports
1/5 In a vibrant homage to Van Gogh's iconic Sunflowers, we introduce the Rosiflowers - a wonder born from the fertile depths of the adipose stromal vascular fraction.
Reposted by Mirian Krystel De Siqueira
Congratulations Mirian @desiqueiramk.bsky.social , the Villanueva and Yang labs at UCLA! Happy to have contributed to such beautiful work highlighting the role of mRNA translation in adipocyte biology.
November 26, 2024 at 1:50 AM
Congratulations Mirian @desiqueiramk.bsky.social , the Villanueva and Yang labs at UCLA! Happy to have contributed to such beautiful work highlighting the role of mRNA translation in adipocyte biology.
Excited to share our latest work in #CellReports highlighting a PPARγ dependent remodeling of translation machinery in adipose progenitors. Amazing collab between our lab Claudio Villanueva, Xia Yang, and @laurahulea.bsky.social !
#obesity #metabolism #translation
www.cell.com/cell-reports...
#obesity #metabolism #translation
www.cell.com/cell-reports...
PPARγ-dependent remodeling of translational machinery in adipose progenitors is impaired in obesity
De Siqueira et al. explore the effects of rosiglitazone on adipose stem cell plasticity,
showing that PPARγ stimulates expression of ribosomal factors in adipocyte precursors.
This leads to translatio...
www.cell.com
November 25, 2024 at 3:46 PM
Excited to share our latest work in #CellReports highlighting a PPARγ dependent remodeling of translation machinery in adipose progenitors. Amazing collab between our lab Claudio Villanueva, Xia Yang, and @laurahulea.bsky.social !
#obesity #metabolism #translation
www.cell.com/cell-reports...
#obesity #metabolism #translation
www.cell.com/cell-reports...
Reposted by Mirian Krystel De Siqueira
PPARγ-dependent remodeling of translational machinery in adipose progenitors is impaired in obesity
www.cell.com/cell-reports...
www.cell.com/cell-reports...
www.cell.com
November 25, 2024 at 1:14 PM
PPARγ-dependent remodeling of translational machinery in adipose progenitors is impaired in obesity
www.cell.com/cell-reports...
www.cell.com/cell-reports...
Reposted by Mirian Krystel De Siqueira
This is a very cool paper that I will add to my Mol Bio course. Specific tRNA recruitment of mRNA degradation complex to ribosome through the P site tRNA interactions with CCR4-NOT complex 1/ www.science.org/doi/10.1126/...
Specific tRNAs promote mRNA decay by recruiting the CCR4-NOT complex to translating ribosomes
The CCR4-NOT complex is a major regulator of eukaryotic messenger RNA (mRNA) stability. Slow decoding during translation promotes association of CCR4-NOT with ribosomes, accelerating mRNA degradation....
www.science.org
November 21, 2024 at 8:03 PM
This is a very cool paper that I will add to my Mol Bio course. Specific tRNA recruitment of mRNA degradation complex to ribosome through the P site tRNA interactions with CCR4-NOT complex 1/ www.science.org/doi/10.1126/...
Reposted by Mirian Krystel De Siqueira
Join our joint meeting on Obesity & Adipose Tissue / MASH! Submit abstracts for short talks by 11:59 PM MST, December 4th. Visit hubs.la/Q02Z4Jlq0 or hubs.la/Q02Z4G770.
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#KSObesity25 #KSMASH25 #obesity #adipose #adiposetissue #adipocyte #metabolicdisease #MASH #NASH #NAFLD #MAFLD #fibrosis
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#KSObesity25 #KSMASH25 #obesity #adipose #adiposetissue #adipocyte #metabolicdisease #MASH #NASH #NAFLD #MAFLD #fibrosis
November 21, 2024 at 6:26 PM
Join our joint meeting on Obesity & Adipose Tissue / MASH! Submit abstracts for short talks by 11:59 PM MST, December 4th. Visit hubs.la/Q02Z4Jlq0 or hubs.la/Q02Z4G770.
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#KSObesity25 #KSMASH25 #obesity #adipose #adiposetissue #adipocyte #metabolicdisease #MASH #NASH #NAFLD #MAFLD #fibrosis
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#KSObesity25 #KSMASH25 #obesity #adipose #adiposetissue #adipocyte #metabolicdisease #MASH #NASH #NAFLD #MAFLD #fibrosis
Reposted by Mirian Krystel De Siqueira
Are you a scientist working or interested in RNA Translation? Join our starter pack and share ideas, methodologies and tips to advance our knowledge in how life came to be!
#RNA #ribosome
go.bsky.app/VN78eTo
#RNA #ribosome
go.bsky.app/VN78eTo
November 24, 2024 at 8:02 PM
Are you a scientist working or interested in RNA Translation? Join our starter pack and share ideas, methodologies and tips to advance our knowledge in how life came to be!
#RNA #ribosome
go.bsky.app/VN78eTo
#RNA #ribosome
go.bsky.app/VN78eTo
Reposted by Mirian Krystel De Siqueira
Working on immunometabolism? Or looking for people who do? Then here’s the starter pack for you! 😊 #Immunosky
go.bsky.app/MvxkA4Q
go.bsky.app/MvxkA4Q
November 24, 2024 at 1:35 PM
Working on immunometabolism? Or looking for people who do? Then here’s the starter pack for you! 😊 #Immunosky
go.bsky.app/MvxkA4Q
go.bsky.app/MvxkA4Q
Reposted by Mirian Krystel De Siqueira
Sharing again as I have added a at least 20 newbies and people how I just found out were here. Just need to get that Giles Yeo over here now go.bsky.app/NRoqMvp
November 16, 2024 at 4:50 PM
Sharing again as I have added a at least 20 newbies and people how I just found out were here. Just need to get that Giles Yeo over here now go.bsky.app/NRoqMvp