Darren Thomson
@darrenthomson.bsky.social
Imaging Scientist @mrccmm MRC Centre for Medical Mycology at UoExeter. Applying and resourcing MYCOscopy to understand human fungal pathogens - always keen to chat
Pinned
Darren Thomson
@darrenthomson.bsky.social
· Feb 18
MBI
Dates: July 28 - August 1, 2025 Introduction to Principles of Microscopy August 4 - 7th, 2025 Advanced Imaging Approaches for Fungal Pathogens
Location: University of Cape Town, South Africa
Venue:...
sites.google.com
🚨🍄🥼Delighted to share the registration page for our Mycology Bioimaging Workshop on "Imaging the Fungal Cell"
W/ pre-AMI Fundamentals workshop @caronjacobs.bsky.social
🗓️ July 28 - Aug 7 2025
🌍 @AMI_microscopy Cape Town, SA
🥼 Hands-on 🔬
Pls RT & Apply here:
sites.google.com/view/mycolog...
W/ pre-AMI Fundamentals workshop @caronjacobs.bsky.social
🗓️ July 28 - Aug 7 2025
🌍 @AMI_microscopy Cape Town, SA
🥼 Hands-on 🔬
Pls RT & Apply here:
sites.google.com/view/mycolog...
Benefits of having collaborators visit, whooo!
January 22, 2026 at 11:29 PM
Benefits of having collaborators visit, whooo!
These pods look so great, need to watch!
Ep 23 - So what actually IS CellPainting anyway? You may have heard of it if you're say, a fan of the @drannecarpenter.bsky.social - @shantanu-singh.cc lab (and who isn't????), but what, you know, is it? Let @erinweisbart.bsky.social break it down for you!
youtu.be/9-d6DoHtgUY
youtu.be/9-d6DoHtgUY
January 20, 2026 at 11:14 PM
These pods look so great, need to watch!
Reposted by Darren Thomson
Candida albicans is usually hanging out with bacterial neighbors, so what happens to the fungus during co-culture?
The bacteria secrete metabolites that induce massive cell wall remodeling that change immune recognition and drug resistance. Read more in our new paper: www.cell.com/current-biol...
The bacteria secrete metabolites that induce massive cell wall remodeling that change immune recognition and drug resistance. Read more in our new paper: www.cell.com/current-biol...
January 20, 2026 at 2:10 PM
Candida albicans is usually hanging out with bacterial neighbors, so what happens to the fungus during co-culture?
The bacteria secrete metabolites that induce massive cell wall remodeling that change immune recognition and drug resistance. Read more in our new paper: www.cell.com/current-biol...
The bacteria secrete metabolites that induce massive cell wall remodeling that change immune recognition and drug resistance. Read more in our new paper: www.cell.com/current-biol...
Reposted by Darren Thomson
Today, our animation synthesizing decades of research on actin-mediated endocytosis in budding yeast was published:
journals.biologists.com/jcs/article/...
The result of a fantastic Iwasa-Drubin lab collaboration.
@margotriggi.bsky.social @jiwasa.bsky.social
movie.biologists.com/video/10.124...
journals.biologists.com/jcs/article/...
The result of a fantastic Iwasa-Drubin lab collaboration.
@margotriggi.bsky.social @jiwasa.bsky.social
movie.biologists.com/video/10.124...
December 2, 2025 at 9:02 PM
Today, our animation synthesizing decades of research on actin-mediated endocytosis in budding yeast was published:
journals.biologists.com/jcs/article/...
The result of a fantastic Iwasa-Drubin lab collaboration.
@margotriggi.bsky.social @jiwasa.bsky.social
movie.biologists.com/video/10.124...
journals.biologists.com/jcs/article/...
The result of a fantastic Iwasa-Drubin lab collaboration.
@margotriggi.bsky.social @jiwasa.bsky.social
movie.biologists.com/video/10.124...
Reposted by Darren Thomson
I stopped doing reviews for Scientific Reports a long time ago as the standard of manuscript I was being sent was what I will politely describe as not great.
But this...
But this...
"Runctitiononal features"? "Medical fymblal"? "1 Tol Line storee"? This gets worse the longer you look at it. But it's got to be good, because it was published in Nature Scientific Reports last week: www.nature.com/articles/s41... h/t @asa.tsbalans.se
November 27, 2025 at 8:51 PM
I stopped doing reviews for Scientific Reports a long time ago as the standard of manuscript I was being sent was what I will politely describe as not great.
But this...
But this...
Reposted by Darren Thomson
We just released a new major version of TrackMate (v8), the cell and organelle tracking plugin of Fiji.
It ships many new features, detailed below, but that are articulated around the following:
It ships many new features, detailed below, but that are articulated around the following:
November 20, 2025 at 11:42 AM
We just released a new major version of TrackMate (v8), the cell and organelle tracking plugin of Fiji.
It ships many new features, detailed below, but that are articulated around the following:
It ships many new features, detailed below, but that are articulated around the following:
Reposted by Darren Thomson
The FPbase spectra viewer just had it's biggest software refactor in 6 years. It *should* be largely invisible to the end user (hopefully just faster and less buggy). Please let me know if you find any issues as you use it!
www.fpbase.org/spectra/
www.fpbase.org/spectra/
FPbase Fluorescence Spectra Viewer
An interactive fluorescence spectra viewer to evaluate the spectral properties of fluorescent proteins, organic dyes, filters, and detectors.
www.fpbase.org
November 4, 2025 at 3:03 PM
The FPbase spectra viewer just had it's biggest software refactor in 6 years. It *should* be largely invisible to the end user (hopefully just faster and less buggy). Please let me know if you find any issues as you use it!
www.fpbase.org/spectra/
www.fpbase.org/spectra/
Reposted by Darren Thomson
Wirshing, Lew et al. @mit.edu address how mothers partition growth equally among their buds in the multi-budding yeast Aureobasidium pullulans. Equal partitioning stems directly from effective equalization of #polarity sites. rupress.org/jcb/article/...
#Cytoskeleton #Development
#Cytoskeleton #Development
September 2, 2025 at 4:15 PM
Wirshing, Lew et al. @mit.edu address how mothers partition growth equally among their buds in the multi-budding yeast Aureobasidium pullulans. Equal partitioning stems directly from effective equalization of #polarity sites. rupress.org/jcb/article/...
#Cytoskeleton #Development
#Cytoskeleton #Development
Does the bass in the dance tent repel the mosquitoes?
Blood, sweat, and beers: investigating mosquito biting preferences amidst noise and intoxication in a cross-sectional cohort study at a large music festival https://www.biorxiv.org/content/10.1101/2025.08.21.671470v1
August 26, 2025 at 6:48 PM
Me and the #MycBio25 cohort had a great time today. Got to see an OpenScope in the wild @uctidm-mu.bsky.social and play for a whole day on an @zeiss-microscopy.bsky.social LSM980 - been 3 years since I’ve used a point scanner confocal on fungi
July 29, 2025 at 5:25 PM
Me and the #MycBio25 cohort had a great time today. Got to see an OpenScope in the wild @uctidm-mu.bsky.social and play for a whole day on an @zeiss-microscopy.bsky.social LSM980 - been 3 years since I’ve used a point scanner confocal on fungi
Me and @caronjacobs.bsky.social are gearing up to deliver this workshop in a few weeks. Excited to discuss the almighty combo of pathogenic fungal cells and the fluorescence microscope!
🚨🍄🥼Delighted to share the registration page for our Mycology Bioimaging Workshop on "Imaging the Fungal Cell"
W/ pre-AMI Fundamentals workshop @caronjacobs.bsky.social
🗓️ July 28 - Aug 7 2025
🌍 @AMI_microscopy Cape Town, SA
🥼 Hands-on 🔬
Pls RT & Apply here:
sites.google.com/view/mycolog...
W/ pre-AMI Fundamentals workshop @caronjacobs.bsky.social
🗓️ July 28 - Aug 7 2025
🌍 @AMI_microscopy Cape Town, SA
🥼 Hands-on 🔬
Pls RT & Apply here:
sites.google.com/view/mycolog...
MBI
Dates: July 28 - August 1, 2025 Introduction to Principles of Microscopy August 4 - 7th, 2025 Advanced Imaging Approaches for Fungal Pathogens
Location: University of Cape Town, South Africa
Venue:...
sites.google.com
July 7, 2025 at 1:33 PM
Me and @caronjacobs.bsky.social are gearing up to deliver this workshop in a few weeks. Excited to discuss the almighty combo of pathogenic fungal cells and the fluorescence microscope!
See my post on web-based deployment of Fiji for image analysis workshops forum.image.sc/t/running-ia...
Advice / input appreciated.
Advice / input appreciated.
Running IA workshop using web-based Fiji deployment (ImJoy)
Hello, I am looking to give image analysis workshops in LMIC locations where deployment of Fiji software is quick and easy. I was thinking of making use of the browser-based IMJOY Fiji platform. Has...
forum.image.sc
July 7, 2025 at 1:31 PM
See my post on web-based deployment of Fiji for image analysis workshops forum.image.sc/t/running-ia...
Advice / input appreciated.
Advice / input appreciated.
Best microscopy based workshop/conference swag?
July 6, 2025 at 12:17 PM
Best microscopy based workshop/conference swag?
Reposted by Darren Thomson
Absolutely thrilled to have been awarded an MRC Programme Grant to study the role of fungi in eczema together with @neil-gow.bsky.social, Helen Alexander and Carsten Flohr (both King's College London). 2 postdoc positions availble at jobs.exeter.ac.uk (Job References Q05626 and Q05627)
June 23, 2025 at 9:29 PM
Absolutely thrilled to have been awarded an MRC Programme Grant to study the role of fungi in eczema together with @neil-gow.bsky.social, Helen Alexander and Carsten Flohr (both King's College London). 2 postdoc positions availble at jobs.exeter.ac.uk (Job References Q05626 and Q05627)
May 24, 2025 at 5:37 PM
I was 3 when we last won the Scottish Cup, the same as my boy! Hope he doesn’t have to wait another 35 yrs!
May 24, 2025 at 5:36 PM
I was 3 when we last won the Scottish Cup, the same as my boy! Hope he doesn’t have to wait another 35 yrs!
Reposted by Darren Thomson
Napoli are crowned Serie A champions.
Goals from Scott McTominay and Romelu Lukaku made sure of it on a dramatic final day
They finish just one point ahead of Inter to win the fourth league title in their history.
Goals from Scott McTominay and Romelu Lukaku made sure of it on a dramatic final day
They finish just one point ahead of Inter to win the fourth league title in their history.
May 23, 2025 at 8:49 PM
Napoli are crowned Serie A champions.
Goals from Scott McTominay and Romelu Lukaku made sure of it on a dramatic final day
They finish just one point ahead of Inter to win the fourth league title in their history.
Goals from Scott McTominay and Romelu Lukaku made sure of it on a dramatic final day
They finish just one point ahead of Inter to win the fourth league title in their history.
Great to have Lucian Duvenage from @mrccmm.bsky.social CapeTown ✈️ to do MYCOscopy w/ @balloulab.bsky.social in Emergomyces Africanus - an emerging fungal pathogen yeast that needs more Mycology bioimaging tools. Here are some Macropahges brimming with them (green yeast)
May 22, 2025 at 3:45 PM
Great to have Lucian Duvenage from @mrccmm.bsky.social CapeTown ✈️ to do MYCOscopy w/ @balloulab.bsky.social in Emergomyces Africanus - an emerging fungal pathogen yeast that needs more Mycology bioimaging tools. Here are some Macropahges brimming with them (green yeast)
Reposted by Darren Thomson
Read preprints. Cite preprints. Email people and tell them you loved their preprint. Email people you hated their preprint. Embrace preprints, preprints are good.
April 22, 2025 at 7:11 PM
Read preprints. Cite preprints. Email people and tell them you loved their preprint. Email people you hated their preprint. Embrace preprints, preprints are good.
Anyone got experience of using Trello w/ TEAMS? Seems like a very nice integration for project tasks / progress contained within MS Office
May 22, 2025 at 3:34 PM
Anyone got experience of using Trello w/ TEAMS? Seems like a very nice integration for project tasks / progress contained within MS Office
Trying again.. any thoughts on below? #fluorescence
Mammalian systems, how stable are #fluorescent protein fusions in living mammalian cells?
It used to be that transfection of epithelial cells were ~20% successfull in a monolayer of cells, which to me was unacceptable. Has this changed?
It used to be that transfection of epithelial cells were ~20% successfull in a monolayer of cells, which to me was unacceptable. Has this changed?
May 15, 2025 at 7:32 AM
Trying again.. any thoughts on below? #fluorescence
Cool/weird that ITV picked this up, usually home to some trashy stuff.
April 7, 2025 at 10:32 PM
Cool/weird that ITV picked this up, usually home to some trashy stuff.
Reposted by Darren Thomson
Delighted to announce that @erinweisbart.bsky.social and I have teamed up to create a new bioimage analysis video podcast called Ask Erin/Dear Beth - you can check it out at the link below! It will highlight common challenges in #bioimageanalysis, as well as our favorite solutions to them. (1/x)
Ask Erin, Dear Beth
On Ask Erin/Dear Beth, bioimage analysis experts Beth Cimini and Erin Weisbart, of the Imaging Platform at the Broad Institute of MIT and Harvard, answer your image analysis questions! Whether it’s ab...
www.youtube.com
April 7, 2025 at 10:11 PM
Delighted to announce that @erinweisbart.bsky.social and I have teamed up to create a new bioimage analysis video podcast called Ask Erin/Dear Beth - you can check it out at the link below! It will highlight common challenges in #bioimageanalysis, as well as our favorite solutions to them. (1/x)
Is the new Lightfield from Zeiss, applicable to volumetric tissue imaging? or is the step sizes more akin to cell biology?
April 2, 2025 at 8:34 AM
Is the new Lightfield from Zeiss, applicable to volumetric tissue imaging? or is the step sizes more akin to cell biology?