Angelo D’Alessandro
@dalessandrolab.bsky.social
Small molecules, big data and something (often red blood cells) in between…
Pinned
Deep Red Blood Cell Proteome Defines the Band 3 N-Terminus Interactome as a Regulator of Hypoxic Adaptation via BLVRB-Dependent S-Nitroso Transfer
Red blood cells (RBCs) have long been regarded as passive oxygen carriers, yet growing evidence reveals a complex, dynamic proteome independent of de novo gene expression. Here, we define the erythroc...
doi.org
Labor of love announcement:
Red blood cells make up 83% of the cells in the human body. Mature RBCs lack nuclei (no gene expression) and organelles, and >90% of their dry weight is hemoglobin. Yet we have lacked a clean, contamination-free map of their proteome.
Until now.
doi.org/10.1101/2025...
Red blood cells make up 83% of the cells in the human body. Mature RBCs lack nuclei (no gene expression) and organelles, and >90% of their dry weight is hemoglobin. Yet we have lacked a clean, contamination-free map of their proteome.
Until now.
doi.org/10.1101/2025...
Reposted by Angelo D’Alessandro
On the outside back cover of issue 1 of Lab on a Chip:
Surface acoustic wave hemolysis assay for evaluating stored red blood cells.
#OpenAccess from Angelo D'Alessandro, Xiaoyun Ding et al @dalessandrolab.bsky.social @cuanschutz.bsky.social @colorado.edu
Read now: pubs.rsc.org/en/content/a...
Surface acoustic wave hemolysis assay for evaluating stored red blood cells.
#OpenAccess from Angelo D'Alessandro, Xiaoyun Ding et al @dalessandrolab.bsky.social @cuanschutz.bsky.social @colorado.edu
Read now: pubs.rsc.org/en/content/a...
January 8, 2026 at 1:51 PM
On the outside back cover of issue 1 of Lab on a Chip:
Surface acoustic wave hemolysis assay for evaluating stored red blood cells.
#OpenAccess from Angelo D'Alessandro, Xiaoyun Ding et al @dalessandrolab.bsky.social @cuanschutz.bsky.social @colorado.edu
Read now: pubs.rsc.org/en/content/a...
Surface acoustic wave hemolysis assay for evaluating stored red blood cells.
#OpenAccess from Angelo D'Alessandro, Xiaoyun Ding et al @dalessandrolab.bsky.social @cuanschutz.bsky.social @colorado.edu
Read now: pubs.rsc.org/en/content/a...
Reposted by Angelo D’Alessandro
Cracking the code of blood: How genetics and metabolism are transforming transfusions
🔗 Read the full Transfusion Today article here: https://isbtweb.foleon.com/transfusion-today/transfusion-today-october-2025/cracking-the-code-of-blood
#TransfusionMedicine #ISBT #TransfusionToday
🔗 Read the full Transfusion Today article here: https://isbtweb.foleon.com/transfusion-today/transfusion-today-october-2025/cracking-the-code-of-blood
#TransfusionMedicine #ISBT #TransfusionToday
Cracking the Code of Blood - Transfusion Today - October 2025
isbtweb.foleon.com
December 19, 2025 at 10:00 AM
Cracking the code of blood: How genetics and metabolism are transforming transfusions
🔗 Read the full Transfusion Today article here: https://isbtweb.foleon.com/transfusion-today/transfusion-today-october-2025/cracking-the-code-of-blood
#TransfusionMedicine #ISBT #TransfusionToday
🔗 Read the full Transfusion Today article here: https://isbtweb.foleon.com/transfusion-today/transfusion-today-october-2025/cracking-the-code-of-blood
#TransfusionMedicine #ISBT #TransfusionToday
Reposted by Angelo D’Alessandro
Reposted by Angelo D’Alessandro
Terrific to have our paper, led by @tjflemin.bsky.social, featured as a plenary article in @bloodjournal.bsky.social today: ashpublications.org/blood/articl...
December 18, 2025 at 6:01 PM
Terrific to have our paper, led by @tjflemin.bsky.social, featured as a plenary article in @bloodjournal.bsky.social today: ashpublications.org/blood/articl...
Reposted by Angelo D’Alessandro
link.springer.com/article/10.1...
The transaminase-ω-amidase pathway senses oxidative stress to control glutamine metabolism and α-ketoglutarate levels in endothelial cells
The transaminase-ω-amidase pathway senses oxidative stress to control glutamine metabolism and α-ketoglutarate levels in endothelial cells
The transaminase-ω-amidase pathway senses oxidative stress to control glutamine metabolism and α-ketoglutarate levels in endothelial cells - The EMBO Journal
Oxidative stress is a major driver of cardiovascular disease; however, the fast changes in cellular metabolism caused by short-lived reactive oxygen species (ROS) remain ill-defined. Here, we characte...
link.springer.com
December 18, 2025 at 8:57 AM
link.springer.com/article/10.1...
The transaminase-ω-amidase pathway senses oxidative stress to control glutamine metabolism and α-ketoglutarate levels in endothelial cells
The transaminase-ω-amidase pathway senses oxidative stress to control glutamine metabolism and α-ketoglutarate levels in endothelial cells
Reposted by Angelo D’Alessandro
Labor of love announcement:
Red blood cells make up 83% of the cells in the human body. Mature RBCs lack nuclei (no gene expression) and organelles, and >90% of their dry weight is hemoglobin. Yet we have lacked a clean, contamination-free map of their proteome.
Until now.
doi.org/10.1101/2025...
Red blood cells make up 83% of the cells in the human body. Mature RBCs lack nuclei (no gene expression) and organelles, and >90% of their dry weight is hemoglobin. Yet we have lacked a clean, contamination-free map of their proteome.
Until now.
doi.org/10.1101/2025...
Deep Red Blood Cell Proteome Defines the Band 3 N-Terminus Interactome as a Regulator of Hypoxic Adaptation via BLVRB-Dependent S-Nitroso Transfer
Red blood cells (RBCs) have long been regarded as passive oxygen carriers, yet growing evidence reveals a complex, dynamic proteome independent of de novo gene expression. Here, we define the erythroc...
doi.org
December 1, 2025 at 2:31 PM
Labor of love announcement:
Red blood cells make up 83% of the cells in the human body. Mature RBCs lack nuclei (no gene expression) and organelles, and >90% of their dry weight is hemoglobin. Yet we have lacked a clean, contamination-free map of their proteome.
Until now.
doi.org/10.1101/2025...
Red blood cells make up 83% of the cells in the human body. Mature RBCs lack nuclei (no gene expression) and organelles, and >90% of their dry weight is hemoglobin. Yet we have lacked a clean, contamination-free map of their proteome.
Until now.
doi.org/10.1101/2025...
Reposted by Angelo D’Alessandro
Inhibition of heme biosynthesis triggers cuproptosis in acute myeloid leukemia: Cell www.cell.com/cell/fulltex...
Inhibition of heme biosynthesis triggers cuproptosis in acute myeloid leukemia
Reduced levels of the essential metabolite heme are a common feature of acute myeloid
leukemia, and consequently, leukemic cells are highly sensitive to inhibition of de
novo heme synthesis. Blockade ...
www.cell.com
November 20, 2025 at 3:29 PM
Inhibition of heme biosynthesis triggers cuproptosis in acute myeloid leukemia: Cell www.cell.com/cell/fulltex...
Now out at @cellpress.bsky.social Cell Genomics, we characterized the genetic architecture of the murine red blood cell proteome using omics profiling in 350 genetically diverse mice.
The goal was to understand how genetic variation shapes RBC metabolism, redox homeostasis, and storage outcomes. 1/n
The goal was to understand how genetic variation shapes RBC metabolism, redox homeostasis, and storage outcomes. 1/n
Genetic architecture of the murine red blood cell proteome reveals central role of hemoglobin beta cysteine 93 in maintaining redox balance
Keele et al. uncover the genetic architecture of the proteome of fresh and stored
red blood cells using a multi-omics analysis of 350 genetically diverse mice. Their
work reveals how a hemoglobin redo...
www.cell.com
November 19, 2025 at 8:24 PM
Now out at @cellpress.bsky.social Cell Genomics, we characterized the genetic architecture of the murine red blood cell proteome using omics profiling in 350 genetically diverse mice.
The goal was to understand how genetic variation shapes RBC metabolism, redox homeostasis, and storage outcomes. 1/n
The goal was to understand how genetic variation shapes RBC metabolism, redox homeostasis, and storage outcomes. 1/n
Reposted by Angelo D’Alessandro
I am very happy to share this paper from a great scientist and surgeon, Cissy Yong, on how warm ischaemia can affect the metabolic profile of a tumour: www.nature.com/articles/s41... 💥 👏
full text access here rdcu.be/ePcEK
full text access here rdcu.be/ePcEK
Tumour sampling conditions perturb the metabolic landscape of clear cell renal cell carcinoma - Nature Communications
Yong et al. highlight how sampling conditions affect metabolic profile in renal cancer, showing that prolonged ischemic exposure disrupts tissue metabolome stability and masks important phenotypes, su...
www.nature.com
November 10, 2025 at 5:09 PM
I am very happy to share this paper from a great scientist and surgeon, Cissy Yong, on how warm ischaemia can affect the metabolic profile of a tumour: www.nature.com/articles/s41... 💥 👏
full text access here rdcu.be/ePcEK
full text access here rdcu.be/ePcEK
Reposted by Angelo D’Alessandro
What a gem from @dudinlab.bsky.social @gautamdey.bsky.social @centriolelab.bsky.social in Cell! Expansion microscopy atlas of >200 eukaryotes comparing cytoskeletal architectures revealing structures not seen before. Stunning visualisation! Exactly the kind of transformative cell biology we need.
October 31, 2025 at 5:45 PM
What a gem from @dudinlab.bsky.social @gautamdey.bsky.social @centriolelab.bsky.social in Cell! Expansion microscopy atlas of >200 eukaryotes comparing cytoskeletal architectures revealing structures not seen before. Stunning visualisation! Exactly the kind of transformative cell biology we need.
Reposted by Angelo D’Alessandro
The October 2025 issue of Transfusion Today is out now!
Discover how developments in Big Data are progressing the field of transfusion medicine by diving into the latest issue of Transfusion Today!
🔗 Read the full issue here: isbtweb.foleon.com/transfusion-...
#TransfusionToday
Discover how developments in Big Data are progressing the field of transfusion medicine by diving into the latest issue of Transfusion Today!
🔗 Read the full issue here: isbtweb.foleon.com/transfusion-...
#TransfusionToday
Cover - Transfusion Today - October 2025
isbtweb.foleon.com
October 31, 2025 at 3:44 PM
The October 2025 issue of Transfusion Today is out now!
Discover how developments in Big Data are progressing the field of transfusion medicine by diving into the latest issue of Transfusion Today!
🔗 Read the full issue here: isbtweb.foleon.com/transfusion-...
#TransfusionToday
Discover how developments in Big Data are progressing the field of transfusion medicine by diving into the latest issue of Transfusion Today!
🔗 Read the full issue here: isbtweb.foleon.com/transfusion-...
#TransfusionToday
Reposted by Angelo D’Alessandro
A cGAS-mediated mechanism in naked mole-rats potentiates DNA repair and delays aging | Science www.science.org/doi/10.1126/...
A cGAS-mediated mechanism in naked mole-rats potentiates DNA repair and delays aging
Efficient DNA repair might make possible the longevity of naked mole-rats. However, whether they have distinctive mechanisms to optimize functions of DNA repair suppressors is unclear. We find that na...
www.science.org
October 10, 2025 at 11:05 AM
A cGAS-mediated mechanism in naked mole-rats potentiates DNA repair and delays aging | Science www.science.org/doi/10.1126/...
Reposted by Angelo D’Alessandro
Elegant work from Kirk Hansen’s lab @cuanschutz.bsky.social Transglutaminase 2 (likely from RBCs) contributes to forming fibrin beta cross-links, even in the absence of FXIII. TG2 increases in plasma in trauma patients with higher injury severity score.
ashpublications.org/blood/articl...
ashpublications.org/blood/articl...
Tissue transglutaminase drives fibrin β-chain cross-linking: a novel fibrin modification observed in trauma patients
Key Points. Trauma patient plasma clots feature entirely novel fibrin β-chain crosslinking not evident in healthy controlsFibrin β-chain crosslinks evident
ashpublications.org
September 22, 2025 at 11:51 PM
Elegant work from Kirk Hansen’s lab @cuanschutz.bsky.social Transglutaminase 2 (likely from RBCs) contributes to forming fibrin beta cross-links, even in the absence of FXIII. TG2 increases in plasma in trauma patients with higher injury severity score.
ashpublications.org/blood/articl...
ashpublications.org/blood/articl...
Reposted by Angelo D’Alessandro
BDH2-driven lysosome-to-mitochondria iron transfer shapes ferroptosis vulnerability of the melanoma cell states
doi.org/10.1038/s422...
doi.org/10.1038/s422...
BDH2-driven lysosome-to-mitochondria iron transfer shapes ferroptosis vulnerability of the melanoma cell states - Nature Metabolism
Rizzollo et al. show that BDH2 participates in iron distribution between cellular compartments, which sets the threshold for the ferroptosis vulnerability of the melanoma cell phenotypes, ultimately a...
doi.org
September 20, 2025 at 6:48 AM
BDH2-driven lysosome-to-mitochondria iron transfer shapes ferroptosis vulnerability of the melanoma cell states
doi.org/10.1038/s422...
doi.org/10.1038/s422...
Patients with SCD transfused with blood stored >30 days have higher iron, cytokines, hemolysis, hypoxia and inflammation markers compared to recipients of RBCs stored <10 days. Metabolites in the bag impact in vivo metabolism (hypoxanthine —> urate). Kudos to Matt Karafin for this clinical trial
RBC units can be stored in the refrigerator for up to 42 days, but it’s unclear how extended storage affects recipients.
@dalessandrolab.bsky.social & team compare short- and long-stored RBC transfusions in patients with sickle cell disease 👇
buff.ly/CWjcWGc
@dalessandrolab.bsky.social & team compare short- and long-stored RBC transfusions in patients with sickle cell disease 👇
buff.ly/CWjcWGc
September 14, 2025 at 12:37 PM
Patients with SCD transfused with blood stored >30 days have higher iron, cytokines, hemolysis, hypoxia and inflammation markers compared to recipients of RBCs stored <10 days. Metabolites in the bag impact in vivo metabolism (hypoxanthine —> urate). Kudos to Matt Karafin for this clinical trial
Another great one from the @cjoneslab.bsky.social !
Excited to share a new publications led by Dr. Cristiana O'Brien showing that circulating lipids levels can be used to predict chemotherapy response in AML patients.
doi.org/10.1182/bloo...
doi.org/10.1182/bloo...
September 5, 2025 at 1:26 AM
Another great one from the @cjoneslab.bsky.social !
Blood donors, like 67% of adult Americans, drink a lot of coffee. However, caffeine may reduce quality of stored RBCs by (i) inhibiting G6PD (~40%); (ii) antagonizing ADORA2b. Likely relevant beyond blood transfusion!
Paper: haematologica.org/haematologic...
news.cuanschutz.edu/news-stories...
Paper: haematologica.org/haematologic...
news.cuanschutz.edu/news-stories...
Study Reveals Caffeine May Undermine Blood Transfusion Effectiveness
Study Reveals Caffeine May Undermine Blood Transfusion Effectiveness
news.cuanschutz.edu
September 4, 2025 at 7:26 PM
Blood donors, like 67% of adult Americans, drink a lot of coffee. However, caffeine may reduce quality of stored RBCs by (i) inhibiting G6PD (~40%); (ii) antagonizing ADORA2b. Likely relevant beyond blood transfusion!
Paper: haematologica.org/haematologic...
news.cuanschutz.edu/news-stories...
Paper: haematologica.org/haematologic...
news.cuanschutz.edu/news-stories...
Reposted by Angelo D’Alessandro
Evidence that mitochondria in macrophages are destroyed by microautophagy
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Evidence that mitochondria in macrophages are destroyed by microautophagy - Nature Communications
Mitophagy, the selective degradation of mitochondria, has been thought to occur via macroautophagy. Here, the authors present data indicating that microautophagy also mediates mitophagy in macrophages...
www.nature.com
September 1, 2025 at 4:59 AM
Evidence that mitochondria in macrophages are destroyed by microautophagy
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Angelo D’Alessandro
Happy to share a new preprint from the Clarke & Vallese (@fravallese.bsky.social) labs reporting #cryoem structures of two RBC stomatin complexes – with AQP1 & UT-B - continuing the SPFH theme from our recent vault preprint! This was a fun one and has been cooking for a while - read on for more! ⬇️
August 30, 2025 at 2:11 PM
Happy to share a new preprint from the Clarke & Vallese (@fravallese.bsky.social) labs reporting #cryoem structures of two RBC stomatin complexes – with AQP1 & UT-B - continuing the SPFH theme from our recent vault preprint! This was a fun one and has been cooking for a while - read on for more! ⬇️
Great job Pavel and team! Thanks for the inclusion
Behind the scenes: first author Guadalupe Roja-Sanchez pipetting away!
August 14, 2025 at 10:07 PM
Great job Pavel and team! Thanks for the inclusion
Iron-loaded Red Blood Cells undergo a lipid peroxidation process akin to ferroptosis during storage in the blood bank. We now show that they also harbor a functional GPX4, and common SNPs, genetic or pharmacological manipulation impact transfusion outcomes www.sciencedirect.com/science/arti...
GPX4 regulates lipid peroxidation and ferroptosis of stored red blood cells
Red blood cell (RBC) membrane lipid peroxidation during blood bank storage profoundly impacts transfusion efficacy; however, the genetic determinants …
www.sciencedirect.com
August 14, 2025 at 6:17 PM
Iron-loaded Red Blood Cells undergo a lipid peroxidation process akin to ferroptosis during storage in the blood bank. We now show that they also harbor a functional GPX4, and common SNPs, genetic or pharmacological manipulation impact transfusion outcomes www.sciencedirect.com/science/arti...
Reposted by Angelo D’Alessandro
Stop 👏 building 👏 python/R👏 packages!
we show that well described methods in academic papers can serve as the specification for an LLM to create methods on demand. This can serve to reduce package maintenance while ensuring accessibility in any programming language
arxiv.org/abs/2507.22324
we show that well described methods in academic papers can serve as the specification for an LLM to create methods on demand. This can serve to reduce package maintenance while ensuring accessibility in any programming language
arxiv.org/abs/2507.22324
From Articles to Code: On-Demand Generation of Core Algorithms from Scientific Publications
Maintaining software packages imposes significant costs due to dependency management, bug fixes, and versioning. We show that rich method descriptions in scientific publications can serve as standalon...
arxiv.org
August 2, 2025 at 5:15 PM
Stop 👏 building 👏 python/R👏 packages!
we show that well described methods in academic papers can serve as the specification for an LLM to create methods on demand. This can serve to reduce package maintenance while ensuring accessibility in any programming language
arxiv.org/abs/2507.22324
we show that well described methods in academic papers can serve as the specification for an LLM to create methods on demand. This can serve to reduce package maintenance while ensuring accessibility in any programming language
arxiv.org/abs/2507.22324
Great work James, Mercedes and teams!
Hidden in the lungs of some breast cancer survivors are tumour cells that can remain dormant for decades — until they one day trigger a relapse
go.nature.com/41iIdJR
go.nature.com/41iIdJR
‘Sleeping’ cancer cells in the lungs can be roused by COVID and flu
Inflammation from the respiratory infections seems to be the culprit, study in mice finds.
go.nature.com
July 30, 2025 at 9:49 PM
Great work James, Mercedes and teams!