Lucas Cheadle
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cheadlelab.bsky.social
Lucas Cheadle
@cheadlelab.bsky.social
150 followers 80 following 11 posts
Associate Professor of Neuroscience @ Cold Spring Harbor Laboratory. Freeman Hrabowski Scholar @ Howard Hughes Medical institute
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Reposted by Lucas Cheadle
dev-journal.bsky.social
A single-cell transcriptomic atlas of sensory-dependent gene expression in developing mouse visual cortex

Read this this #LifelongDevSI #OA Techniques and Resources Article by Andre M. Xavier, Qianyu Lin, Chris J. Kang, Lucas Cheadle (@cheadlelab.bsky.social)

doi.org/10.1242/dev....
Figure 1 - Experimental design and introduction to the single-nucleus RNA sequencing dataset. (A) Schematic illustrating the late dark-rearing (LDR) paradigm and the workflow of the single-nucleus RNA sequencing (snRNAseq) experiments. (B) Quantification of Fos mRNA expression in sensory deprived (LDR) mice and in mice acutely exposed to light for between 15 min and 2 h, with stimulation timepoints labeled as follows: LDR15m (15 min of light), LDR30m (30 min), LDR1h (1 h) and LDR2h (2 h). Fos expression assessed by qPCR and normalized to Gapdh. Values plotted are additionally normalized to the LDR condition. (C) qPCR quantification of Jun mRNA expression (normalized to Gapdh) in V1 across all timepoints. Data obtained by qPCR and values plotted are normalized to LDR. In B and C, data are mean±s.e.m. n=3 mice per condition. One-way ANOVA followed by Tukey's post hoc test: *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001. (D) Example confocal images of V1 in sections from a sensory deprived mouse (LDR) and a mouse re-exposed to light for 30 min (LDR30m). Fos mRNA (red), Jun mRNA (green) and DAPI (blue). Scale bar: 44 µm. (E) UMAP plot illustrating the 118,529 nuclei in the dataset categorized by general cell class: excitatory neurons (blue), inhibitory neurons (pink) and glia (green). (F) UMAP plot with all 16 clusters colored and labeled by cell type.
November 3, 2025 at 3:59 PM
cheadlelab.bsky.social
Thrilled to welcome the new FHS cohort… including these newly selected McKnight Scholars!
hhmi.org HHMI @hhmi.org · Jun 20
Congrats to HHMI Freeman Hrabowski Scholars @channyskye.bsky.social , Josefina del Mármol (@delmarmollab.bsky.social), @yvetteefisher.bsky.social and Theanne Griffith (@doctheagrif.bsky.social), named 2025 McKnight Scholars for their demonstrated commitment to neuroscience & mentoring others! 🌟
June 20, 2025 at 6:39 PM
cheadlelab.bsky.social
Love this
bethstevenslab.bsky.social Beth Stevens @bethstevenslab.bsky.social · May 9
Let’s get the word out about the importance of federal funding of curiosity -driven science
We all have similar stories to share
harvardbrainsci.bsky.social Harvard Brain Science Initiative @harvardbrainsci.bsky.social · May 7
Protecting the Brain Requires Persistence
@bethstevenslab.bsky.social, NIH-supported investigator of Alzheimer’s and other disorders, explains how one discovery can lead to another.
news.harvard.edu/gazette/stor...
May 10, 2025 at 5:32 PM
cheadlelab.bsky.social
Hosting Dr. Kaf Dzirasa @hhmi.org for a seminar at CSHL and getting to spend time w/fellow FHS @ishmailsaboor.bsky.social in the process has made this week a highlight of my year. Feeling seriously inspired today.
ishmailsaboor.bsky.social Ishmail Abdus-Saboor @ishmailsaboor.bsky.social · Apr 24
Groundbreaking talk from Prof. Kaf Dzirasa today at Cold Spring Harbor. Thanks @cheadlelab.bsky.social for allowing me to crash Kaf’s talk and your lab meeting. Feeling inspired!
April 24, 2025 at 6:23 PM
cheadlelab.bsky.social
journals.biologists.com/dev/article/...

New work from the lab characterizing sensory-dependent transcriptomes in mouse V1 across neuronal and glial cell types. Some fun nuggets in here, and all data publicly available for download and re-analysis. Happy gene hunting!
A single-cell transcriptomic atlas of sensory-dependent gene expression in developing mouse visual cortex
Summary: Single-cell RNA sequencing of developing mouse visual cortex uncovers cell type-specific gene programs induced by sensory experience. All cell types mounted transcriptional responses to the e...
journals.biologists.com
March 27, 2025 at 4:46 PM
Reposted by Lucas Cheadle
michellemonje.bsky.social
Delighted to share the published version of this work from brilliant #CancerNeuroscience post-doc Tara Barron detailing GABAergic neuron-to-glioma synapses in #DIPG/#DMG. 🧵
Open access link:
www.nature.com/articles/s41...
February 19, 2025 at 9:41 PM
cheadlelab.bsky.social
Dear friends and colleagues: nice to see you here where the skies are bluer. Follow me for updates on our research into neuro-immune interactions in brain development, function, and disease. @cshlnews.bsky.social @hhmi.bsky.social
January 27, 2025 at 4:47 PM