Bacteriophage ΦKZ is a massive bacterial virus with potential as a therapy for Pseudomonas aeruginosa, which forms a bacteriophage nucleus in infected cells to exclude the host immune system, and transcribes its genes with its own, non-canonical multi-subunit RNA polymerase. The structure of this polymerase in the process of binding an analogue of its cognate promoter is resolved, revealing an unanticipated method of promoter consensus recognition involving the β-like, rather than the σ-factor-like subunit.

This study presents the crystal structure of human rhinovirus (hRV)-B14 3C protease bound to AG7404, a broad-spectrum antiviral inhibitor, at a resolution of 2.11 Å. Antiviral assays and in silico studies provide structural insights into AG7404's efficacy and binding mechanism against hRV-B14, hRV-A16 and hRV-A21 3C proteases.

The structures of three doubly-acyl­ated 4-amino­anti­pyrine com­pounds where the aryl substituent is varied are reported and analysed in terms of their relative conformation, inter­molecular inter­actions and overall packing. The com­pounds were crystallized using the encapsulated nanodroplet crystallization (ENaCt) protocol.

Three pyridine-based halide-bibridged polymers have been synthesized and their structures determined. The effect of the size of the halides on the crystal structures has been investigated.

The mol­ecular structures of two novel cobalt amino­diphosphine (PNP) com­plexes are reported, featuring variation in the N-atom substituent. A Cambridge Structural Database (CSD) survey, along with a noncovalent inter­action (NCI) analysis of the analogous [TMCln(PNP)2] (where TM = transition metal and n = 1 or 2) core, revealed an inverse correlation between the P—TM—P bite angles and N⋯TM contact distances.

CryoSift is a CNN-based tool that assesses the quality of 2D class averages. The incorporation of CryoSift into cryoSPARC workflows enables automated particle-stack cleaning.

Crystals of flocculosin-A, the major antifungus compound from Anthracocystis flocculosa, are optimised using a cyclic temperature control.

This study highlights how solvent properties, host–guest interaction energies and packing preferences govern the solid-state organization of tert-butyl­calix[6]arene, a macrocycle of importance in separation, molecular recognition and supramolecular chemistry.

The crystal structure of a reagent frequently used for the preparation of buffer solutions is reported, showing that the mol­ecule lies on a crystallographic mirror plane, and has a bent conformation.

The title compound is a hydrated, partially protected derivative of d-gluco­pyran­ose. Inter­molecular inter­actions connect the entities of the asymmetric unit to a three-dimensional network.

In the title compound, much of the mol­ecule (excluding the pyridinic ring) is approximately planar. In the extended structure, various hydrogen bonds combine to form a layer structure parallel to (111).

An advanced X-ray ptychography system has been developed that facilitates nanoscale imaging across both tender and hard X-ray regions using a single optical setup.

A one-dimensional lanthanum coordination polymer based on 3,6-di­chloro­phthalate has been prepared by microwaves-assisted synthesis and structurally described.

X-ray crystal structures of glycosyltransferase C from the Limosilactobacillus reuteri 100-23 (LrGtfC100-23) accessory secretion system were determined in its apo form and in complexes with UDP and with UDP-N-acetylglucosamine, which revealed candidate residues involved in strain-specific recognition of UDP-sugars. Site-directed mutagenesis of these residues led to a switch of the UDP-sugar binding specificities of LrGtfC100-23 and the highly homologous LrGtfC from L. reuteri ATCC 53608.

A novel small-angle X-ray scattering modelling approach is introduced for the analysis of precipitation behaviour in metallic systems exhibiting scattering signals with streaks originating from the embedding matrix. This approach enables in situ tracking of the microstructural evolution, such as the volume fraction and size of second phases, during continuous heating, and it is showcased for a near-α Ti alloy.

The title acridine derivative, which has two 2-methyl­but-3-yn-2-ol moieties at the 2,7-positions, was synthesized by Sonogashira coupling reaction. In crystal, a columnar structure is formed by the π–π stacking of acridine units. The 2-methyl­but-3-yn-2-ol moieties form inter­molecular hydrogen bonds.

The lithium halide dimethyl ether (DME) adducts [Li2Cl2(DME)4] and [Li2Br2(DME)4] form dimeric units linked into layers by CH3⋯Cl tetrel bonds or CH3⋯CH3, CH3⋯O and CH3⋯Br contacts, illustrating the coordination ability of dimethyl ether and its role in directing supra­molecular assembly.

The two compounds Ag3ISO4 and Ag4I2SO4, comprising Ag and I atoms and SO4 tetra­hedra, exhibit characteristic silver arrangements, with Ag3ISO4 containing triangularly arranged Ag atoms in zigzag ladder chains along the a-axis direction and Ag4I2SO4 featuring discrete Ag4 clusters.

The newest seven members of the Editorial Board of Journal of Synchrotron Radiation are introduced.