Structural Biology Communications
@actacrystf.iucr.org
Acta Crystallographica Section F: Structural Biology Communications is a structural biology communications journal published by the IUCr. http://journals.iucr.org/f
Zang, Jiang and Zhou: Structural analysis of the tetrahydrobiopterin glucosyltransferase PsBGluT from Pseudanabaena sp. Chao 1811 ##Tetrahydrobiopterin##GTBGlycosyltransferase##Crystallography... ##IUCrhttps://journals.iucr.org/paper?S2053230X25009446
Structural analysis of the tetrahydrobiopterin glucosyltransferase PsBGluT from Pseudanabaena sp. Chao 1811
This study advances our understanding of pteridine glycosylation and also provides a structural basis for investigating photosynthetic signaling pathways in cyanobacteria.
journals.iucr.org
November 14, 2025 at 1:00 AM
Zang, Jiang and Zhou: Structural analysis of the tetrahydrobiopterin glucosyltransferase PsBGluT from Pseudanabaena sp. Chao 1811 ##Tetrahydrobiopterin##GTBGlycosyltransferase##Crystallography... ##IUCrhttps://journals.iucr.org/paper?S2053230X25009446
A gentle, automated, inexpensive and open-source device and method for changing the ambient solution of a macromolecular crystal #Crystal #Solution #Cracking doi.org/10.1107/S205...
November 13, 2025 at 3:29 PM
A gentle, automated, inexpensive and open-source device and method for changing the ambient solution of a macromolecular crystal #Crystal #Solution #Cracking doi.org/10.1107/S205...
A kinetically impaired Gln143Asn MnSOD variant is used to trap and explore additional hydrogen peroxide-binding sites beyond the second-shell solvent gate #ManganeseSuperoxideDismutases #PeroxideBinding #Metalloenzymes doi.org/10.1107/S205...
November 12, 2025 at 4:06 PM
A kinetically impaired Gln143Asn MnSOD variant is used to trap and explore additional hydrogen peroxide-binding sites beyond the second-shell solvent gate #ManganeseSuperoxideDismutases #PeroxideBinding #Metalloenzymes doi.org/10.1107/S205...
Sean P. Zupko et al.: Crystal structure of a short-chain dehydrogenase from Brucella ovis with apo and coenzyme NAD+-bound protomer chains ##RedoxChemistry##ResearchExperienceForUndergraduates@suny_cortland@UMNews...##IUCrhttps://journals.iucr.org/paper?S2053230X25009227
Crystal structure of a short-chain dehydrogenase from Brucella ovis with apo and coenzyme NAD+-bound protomer chains
This article describes the structural characterization of a short-chain dehydrogenase from the pathogenic bacterium Brucella ovis. In this manuscript, two structures are described: the structure of the apo protein and a structure containing the coenzyme NAD+.
journals.iucr.org
November 12, 2025 at 1:00 AM
Sean P. Zupko et al.: Crystal structure of a short-chain dehydrogenase from Brucella ovis with apo and coenzyme NAD+-bound protomer chains ##RedoxChemistry##ResearchExperienceForUndergraduates@suny_cortland@UMNews...##IUCrhttps://journals.iucr.org/paper?S2053230X25009227
The first crystal structure of FucWf4, a newly characterized fucosidase that has structural similarity to GH29 enzymes but is currently unclassified, is presented #GlycosideHydrolases #Fucoidan #Fucosidase doi.org/10.1107/S205...
November 11, 2025 at 1:45 PM
The first crystal structure of FucWf4, a newly characterized fucosidase that has structural similarity to GH29 enzymes but is currently unclassified, is presented #GlycosideHydrolases #Fucoidan #Fucosidase doi.org/10.1107/S205...
Structural investigations of unliganded murine KHK-A revealed the adoption of two conformations similar to those adopted by the human ortholog, suggesting this structural feature is conserved across species #PfkBFamily #Moonlighting #ProteinKinases doi.org/10.1107/S205...
November 10, 2025 at 2:16 PM
Structural investigations of unliganded murine KHK-A revealed the adoption of two conformations similar to those adopted by the human ortholog, suggesting this structural feature is conserved across species #PfkBFamily #Moonlighting #ProteinKinases doi.org/10.1107/S205...
Jan-Hannes Schäfer et al.: CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection ##Automation##DataProcessing##CryoEM@umich...##IUCrhttps://journals.iucr.org/paper?S2053230X25008866
CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection
CryoSift is a CNN-based tool that assesses the quality of 2D class averages. The incorporation of CryoSift into cryoSPARC workflows enables automated particle-stack cleaning.
journals.iucr.org
November 8, 2025 at 1:00 AM
Jan-Hannes Schäfer et al.: CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection ##Automation##DataProcessing##CryoEM@umich...##IUCrhttps://journals.iucr.org/paper?S2053230X25008866
Reposted by Structural Biology Communications
CryoSift is now out @actacrystf.iucr.org doi.org/10.1107/S205... Thanks to all involved. Happy processing!
November 7, 2025 at 4:58 PM
CryoSift is now out @actacrystf.iucr.org doi.org/10.1107/S205... Thanks to all involved. Happy processing!
November issue out now: journals.iucr.org/f/issues/202...
On the cover: the structure of the kinetically slow and catalytically impaired Gln143Asn mutant of human mitochondrial MnSOD leads to the observation of transient hydrogen peroxide-bound states. More at shorturl.at/41Mb9
On the cover: the structure of the kinetically slow and catalytically impaired Gln143Asn mutant of human mitochondrial MnSOD leads to the observation of transient hydrogen peroxide-bound states. More at shorturl.at/41Mb9
November 5, 2025 at 1:25 PM
November issue out now: journals.iucr.org/f/issues/202...
On the cover: the structure of the kinetically slow and catalytically impaired Gln143Asn mutant of human mitochondrial MnSOD leads to the observation of transient hydrogen peroxide-bound states. More at shorturl.at/41Mb9
On the cover: the structure of the kinetically slow and catalytically impaired Gln143Asn mutant of human mitochondrial MnSOD leads to the observation of transient hydrogen peroxide-bound states. More at shorturl.at/41Mb9
Medhanjali Dasgupta et al.: High-resolution X-ray structure of Gln143Asn manganese superoxide dismutase captures multiple hydrogen peroxide-binding sites ##Metalloenzymes##PeroxideBinding@unmc...##IUCrhttps://journals.iucr.org/paper?S2053230X25009045
High-resolution X-ray structure of Gln143Asn manganese superoxide dismutase captures multiple hydrogen peroxide-binding sites
A high-resolution X-ray structure of a Gln143Asn variant of manganese superoxide dismutase (MnSOD) reveals multiple hydrogen peroxide-binding sites beyond the canonical LIG and PEO binding positions within the active site. These findings expand the known landscape of product peroxide-bound states in MnSOD.
journals.iucr.org
October 24, 2025 at 12:00 AM
Medhanjali Dasgupta et al.: High-resolution X-ray structure of Gln143Asn manganese superoxide dismutase captures multiple hydrogen peroxide-binding sites ##Metalloenzymes##PeroxideBinding@unmc...##IUCrhttps://journals.iucr.org/paper?S2053230X25009045
Reposted by Structural Biology Communications
One of the coolest things going on here is that the GitHub repos contain Jupyter notebooks for you to try their software. In case you don't have Jupyter installed or don't know what it is: you can always upload the notebooks to Google Colab and run everything on their cloud services :)
Tools are presented that are useful for collating existing public cryoEM data sets and/or creating new synthetic cryoEM data sets to aid the development of novel data processing and interpretation algorithms #CryoEM #ElectronCryomicroscopy #Python doi.org/10.1107/S205...
October 22, 2025 at 3:58 PM
One of the coolest things going on here is that the GitHub repos contain Jupyter notebooks for you to try their software. In case you don't have Jupyter installed or don't know what it is: you can always upload the notebooks to Google Colab and run everything on their cloud services :)
Tools are presented that are useful for collating existing public cryoEM data sets and/or creating new synthetic cryoEM data sets to aid the development of novel data processing and interpretation algorithms #CryoEM #ElectronCryomicroscopy #Python doi.org/10.1107/S205...
October 22, 2025 at 2:16 PM
Tools are presented that are useful for collating existing public cryoEM data sets and/or creating new synthetic cryoEM data sets to aid the development of novel data processing and interpretation algorithms #CryoEM #ElectronCryomicroscopy #Python doi.org/10.1107/S205...
D-Aspartate oxidase from Cryptococcus humicola UJ1 reveals a unique homotetrameric organization due to distinct interfacial loop arrangements #DAspartateOxidase #CrystalStructure #FADDependentEnzymes doi.org/10.1107/S205...
October 21, 2025 at 3:00 PM
D-Aspartate oxidase from Cryptococcus humicola UJ1 reveals a unique homotetrameric organization due to distinct interfacial loop arrangements #DAspartateOxidase #CrystalStructure #FADDependentEnzymes doi.org/10.1107/S205...
Brooke Bailey et al.: Structural insights into a fucosidase involved in fucoidan degradation ##GlycosideHydrolases##Fucoidan##Fucosidase... ##IUCrhttps://journals.iucr.org/paper?S2053230X25008842
Structural insights into a fucosidase involved in fucoidan degradation
We present the first detailed structure of a newly discovered fucosidase that has been shown to remove terminal fucose from fucoidan, uncovering unique features that set it apart from other known fucosidases and suggesting functional diversity among its homologs.
journals.iucr.org
October 21, 2025 at 12:00 AM
Brooke Bailey et al.: Structural insights into a fucosidase involved in fucoidan degradation ##GlycosideHydrolases##Fucoidan##Fucosidase... ##IUCrhttps://journals.iucr.org/paper?S2053230X25008842
The structure of B. subtilis DegQ from the DegS–DegU two-component system provide structural insight into DegQ oligomerization and its potential role in modulating DegS autophosphorylation and DegU binding #BacillusSubtilis #CrystalStructure #DegQ doi.org/10.1107/S205...
October 20, 2025 at 12:52 PM
The structure of B. subtilis DegQ from the DegS–DegU two-component system provide structural insight into DegQ oligomerization and its potential role in modulating DegS autophosphorylation and DegU binding #BacillusSubtilis #CrystalStructure #DegQ doi.org/10.1107/S205...
Bae, Allen and Tolan: Conformational changes in ketohexokinase are conserved across isozymes and species ##PfkBFamily##Moonlighting##ProteinKinases... ##IUCrhttps://journals.iucr.org/paper?S2053230X25008428
Conformational changes in ketohexokinase are conserved across isozymes and species
Mouse ketohexokinase A undergoes a conformational change during the catalytic cycle. The conformational change is consistent between the two isozymes, A and C, and across species.
journals.iucr.org
October 11, 2025 at 12:00 AM
Bae, Allen and Tolan: Conformational changes in ketohexokinase are conserved across isozymes and species ##PfkBFamily##Moonlighting##ProteinKinases... ##IUCrhttps://journals.iucr.org/paper?S2053230X25008428
Reposted by Structural Biology Communications
Great to see this article by @guptapenn.bsky.social is out! Kushol helped us interview @xtalmaker.bsky.social Vivian Stojanoff and @gabbygrowsxtals.bsky.social who published an article on careful sample preparation in crystallisation experiments, also part of #SAMPREP
www.youtube.com/watch?v=m34R...
www.youtube.com/watch?v=m34R...
October 10, 2025 at 10:07 AM
Great to see this article by @guptapenn.bsky.social is out! Kushol helped us interview @xtalmaker.bsky.social Vivian Stojanoff and @gabbygrowsxtals.bsky.social who published an article on careful sample preparation in crystallisation experiments, also part of #SAMPREP
www.youtube.com/watch?v=m34R...
www.youtube.com/watch?v=m34R...
Four cyanobacterial glycosyl transferase family 35 α-glucan phosphorylases of types I and II were biochemically characterized and one was crystallized and crystallographically analyzed #Cyanobacteria #CyanobacterialStarch #Glycogen doi.org/10.1107/S205...
October 9, 2025 at 3:04 PM
Four cyanobacterial glycosyl transferase family 35 α-glucan phosphorylases of types I and II were biochemically characterized and one was crystallized and crystallographically analyzed #Cyanobacteria #CyanobacterialStarch #Glycogen doi.org/10.1107/S205...
The higher resolution, significantly improved apo crystal structure of mouse gasdermin D will be beneficial for structure-based drug-design approaches towards this important pharmacological target #Pyroptosis #Inflammation #GasderminD doi.org/10.1107/S205...
October 8, 2025 at 1:35 PM
The higher resolution, significantly improved apo crystal structure of mouse gasdermin D will be beneficial for structure-based drug-design approaches towards this important pharmacological target #Pyroptosis #Inflammation #GasderminD doi.org/10.1107/S205...
The SAMPREP workshop at the 2023 ACA Annual Meeting in Baltimore was designed to address the need to consoiidate cross-disciplinary best practices for sample preparation into a single forum #SamplePreparation #StructuralBiology #CryoEM doi.org/10.1107/S205...
October 7, 2025 at 12:48 PM
The SAMPREP workshop at the 2023 ACA Annual Meeting in Baltimore was designed to address the need to consoiidate cross-disciplinary best practices for sample preparation into a single forum #SamplePreparation #StructuralBiology #CryoEM doi.org/10.1107/S205...
Reposted by Structural Biology Communications
Take a look at our October issue: journals.iucr.org/f/issues/202...
The cover shows the crystal structure of Cryptococcus humicola D-aspartate oxidase, which has a unique homotetrameric architecture in contrast to other known D-amino-acid oxidases. Read more at doi.org/10.1107/S205...
The cover shows the crystal structure of Cryptococcus humicola D-aspartate oxidase, which has a unique homotetrameric architecture in contrast to other known D-amino-acid oxidases. Read more at doi.org/10.1107/S205...
October 2, 2025 at 1:45 PM
Take a look at our October issue: journals.iucr.org/f/issues/202...
The cover shows the crystal structure of Cryptococcus humicola D-aspartate oxidase, which has a unique homotetrameric architecture in contrast to other known D-amino-acid oxidases. Read more at doi.org/10.1107/S205...
The cover shows the crystal structure of Cryptococcus humicola D-aspartate oxidase, which has a unique homotetrameric architecture in contrast to other known D-amino-acid oxidases. Read more at doi.org/10.1107/S205...
Douglas H. Juers et al.: Automated gradient equilibration of macromolecular crystals to new solution conditions ##Crystal##Solution##Cracking... ##IUCrhttps://journals.iucr.org/paper?S2053230X25008398
Automated gradient equilibration of macromolecular crystals to new solution conditions
A device and method for gentle, automated and inexpensive solution exchange for macromolecular crystals is described.
journals.iucr.org
October 4, 2025 at 12:00 AM
Douglas H. Juers et al.: Automated gradient equilibration of macromolecular crystals to new solution conditions ##Crystal##Solution##Cracking... ##IUCrhttps://journals.iucr.org/paper?S2053230X25008398
Take a look at our October issue: journals.iucr.org/f/issues/202...
The cover shows the crystal structure of Cryptococcus humicola D-aspartate oxidase, which has a unique homotetrameric architecture in contrast to other known D-amino-acid oxidases. Read more at doi.org/10.1107/S205...
The cover shows the crystal structure of Cryptococcus humicola D-aspartate oxidase, which has a unique homotetrameric architecture in contrast to other known D-amino-acid oxidases. Read more at doi.org/10.1107/S205...
October 2, 2025 at 1:45 PM
Take a look at our October issue: journals.iucr.org/f/issues/202...
The cover shows the crystal structure of Cryptococcus humicola D-aspartate oxidase, which has a unique homotetrameric architecture in contrast to other known D-amino-acid oxidases. Read more at doi.org/10.1107/S205...
The cover shows the crystal structure of Cryptococcus humicola D-aspartate oxidase, which has a unique homotetrameric architecture in contrast to other known D-amino-acid oxidases. Read more at doi.org/10.1107/S205...
Reposted by Structural Biology Communications
I'm incredibly proud to present this @actacrystf.iucr.org interview with Alex Mendez and Cydni Bolling on their first paper ever! The interview was co-led by Dr Graham Chakafana from Hampton University in the USA, who supervised the authors' undergraduate research.
www.youtube.com/watch?v=5rjI...
www.youtube.com/watch?v=5rjI...
Structure of Plasmodium vivax N-myristoyltransferase with the NMT inhibitor IMP-1088
YouTube video by International Union of Crystallography
www.youtube.com
September 24, 2025 at 4:31 PM
I'm incredibly proud to present this @actacrystf.iucr.org interview with Alex Mendez and Cydni Bolling on their first paper ever! The interview was co-led by Dr Graham Chakafana from Hampton University in the USA, who supervised the authors' undergraduate research.
www.youtube.com/watch?v=5rjI...
www.youtube.com/watch?v=5rjI...
The structure of a 7-substitution mutant of hydroxynitrile lyase shows that targeted amino-acid substitutions can shift catalytic geometries towards those of evolutionarily related enzymes #EngineeredProteins #HydroxynitrileLyases #HydrolaseFold doi.org/10.1107/S205...
September 15, 2025 at 3:14 PM
The structure of a 7-substitution mutant of hydroxynitrile lyase shows that targeted amino-acid substitutions can shift catalytic geometries towards those of evolutionarily related enzymes #EngineeredProteins #HydroxynitrileLyases #HydrolaseFold doi.org/10.1107/S205...