Winkler Laboratory
@winklerlab.bsky.social
We are the Winkler Laboratory at the UKHD and DKFZ Heidelberg
Our focus: #cancerneuroscience of #braintumors ( #glioblastoma #brainmetastases), and beyond.
We use #intravitalimaging , #calciumimaging, #preclinicalmodels and more, to tackle these diseases
Our focus: #cancerneuroscience of #braintumors ( #glioblastoma #brainmetastases), and beyond.
We use #intravitalimaging , #calciumimaging, #preclinicalmodels and more, to tackle these diseases
In summary, we introduce a scalable anti-TM #drugscreening platform and reveal PKC activation as a TM master-regulator.
TM targeting disconnects tumor networks and sensitizes #GBM to #therapy.
#Translational #CancerNeuroscience
TM targeting disconnects tumor networks and sensitizes #GBM to #therapy.
#Translational #CancerNeuroscience
October 9, 2025 at 8:52 PM
In summary, we introduce a scalable anti-TM #drugscreening platform and reveal PKC activation as a TM master-regulator.
TM targeting disconnects tumor networks and sensitizes #GBM to #therapy.
#Translational #CancerNeuroscience
TM targeting disconnects tumor networks and sensitizes #GBM to #therapy.
#Translational #CancerNeuroscience
Spatial multi-omics analysis (with COMET) of brains revealed that cells in the TPPB/RT combination treatment group exhibited fewer and shorter TMs, reduced proliferation, increased cell death, and decreased TTYH1 expression (a driver of TM invasion)
#GBM #COMET #Lunaphore @bio-techne.bsky.social
#GBM #COMET #Lunaphore @bio-techne.bsky.social
October 9, 2025 at 8:52 PM
Spatial multi-omics analysis (with COMET) of brains revealed that cells in the TPPB/RT combination treatment group exhibited fewer and shorter TMs, reduced proliferation, increased cell death, and decreased TTYH1 expression (a driver of TM invasion)
#GBM #COMET #Lunaphore @bio-techne.bsky.social
#GBM #COMET #Lunaphore @bio-techne.bsky.social
Intravital 2-photon microscopy shows how TPPB breaks tumor networks and boosts radiotherapy in mice.
Control+RT → network persists.
TPPB+RT → fewer tumor microtubes, network collapse, regional regression!
#Radiotherapy #IntravitalMicroscopy #preclinicalmodels #invivo
Control+RT → network persists.
TPPB+RT → fewer tumor microtubes, network collapse, regional regression!
#Radiotherapy #IntravitalMicroscopy #preclinicalmodels #invivo
October 9, 2025 at 8:52 PM
Intravital 2-photon microscopy shows how TPPB breaks tumor networks and boosts radiotherapy in mice.
Control+RT → network persists.
TPPB+RT → fewer tumor microtubes, network collapse, regional regression!
#Radiotherapy #IntravitalMicroscopy #preclinicalmodels #invivo
Control+RT → network persists.
TPPB+RT → fewer tumor microtubes, network collapse, regional regression!
#Radiotherapy #IntravitalMicroscopy #preclinicalmodels #invivo
The efficacy of TPPB was substantiated in 3D human glioma cortical spheroids and corroborated by longitudinal intravital imaging of mouse brains and histological analysis.
Precisely, application of TPPB in vivo with radiotherapy reduced tumor burden and conferred a survival benefit vs RT alone.
Precisely, application of TPPB in vivo with radiotherapy reduced tumor burden and conferred a survival benefit vs RT alone.
October 9, 2025 at 8:52 PM
The efficacy of TPPB was substantiated in 3D human glioma cortical spheroids and corroborated by longitudinal intravital imaging of mouse brains and histological analysis.
Precisely, application of TPPB in vivo with radiotherapy reduced tumor burden and conferred a survival benefit vs RT alone.
Precisely, application of TPPB in vivo with radiotherapy reduced tumor burden and conferred a survival benefit vs RT alone.
Likewise, they inhibited global Ca²⁺ activity and pacemaker-like cells fell silent.
#PacemakerCell #calciumimaging #translationalresearch #periodicity
#PacemakerCell #calciumimaging #translationalresearch #periodicity
October 9, 2025 at 8:52 PM
Likewise, they inhibited global Ca²⁺ activity and pacemaker-like cells fell silent.
#PacemakerCell #calciumimaging #translationalresearch #periodicity
#PacemakerCell #calciumimaging #translationalresearch #periodicity
PKC modulators emerged as the lead class of anti-TM drugs. Their members TPPB & PMA reduced TMs per cell and TM length without overt cytotoxicity.
#PKC #tumornetworks
#PKC #tumornetworks
October 9, 2025 at 8:52 PM
PKC modulators emerged as the lead class of anti-TM drugs. Their members TPPB & PMA reduced TMs per cell and TM length without overt cytotoxicity.
#PKC #tumornetworks
#PKC #tumornetworks
We built a machine-learning based 2D/3D morphometry pipeline to quantify TMs and network activity in 2D GB stem-like cells, 3D human brain spheroids (hGliCS), and mice.
Using this platform, we screened 71 compounds to identify the most potent ones.
#TumorMicrotubes #drugrepurposing
Using this platform, we screened 71 compounds to identify the most potent ones.
#TumorMicrotubes #drugrepurposing
October 9, 2025 at 8:52 PM
We built a machine-learning based 2D/3D morphometry pipeline to quantify TMs and network activity in 2D GB stem-like cells, 3D human brain spheroids (hGliCS), and mice.
Using this platform, we screened 71 compounds to identify the most potent ones.
#TumorMicrotubes #drugrepurposing
Using this platform, we screened 71 compounds to identify the most potent ones.
#TumorMicrotubes #drugrepurposing
Glioblastoma cells extend TMs to invade and wire into resilient networks.
Particularly these network-integrated cells communicate via Ca²⁺waves and resist standard therapy.
Thus, targeting TMs holds promise to overcome the resistance.
#Glioblastoma #CancerNeuroscience #cancerresearch
Particularly these network-integrated cells communicate via Ca²⁺waves and resist standard therapy.
Thus, targeting TMs holds promise to overcome the resistance.
#Glioblastoma #CancerNeuroscience #cancerresearch
October 9, 2025 at 8:52 PM
Glioblastoma cells extend TMs to invade and wire into resilient networks.
Particularly these network-integrated cells communicate via Ca²⁺waves and resist standard therapy.
Thus, targeting TMs holds promise to overcome the resistance.
#Glioblastoma #CancerNeuroscience #cancerresearch
Particularly these network-integrated cells communicate via Ca²⁺waves and resist standard therapy.
Thus, targeting TMs holds promise to overcome the resistance.
#Glioblastoma #CancerNeuroscience #cancerresearch
How to disconnect therapy-resistant tumor networks in glioblastoma?
In our paper, online @Cancer Discovery, we built an anti-tumor microtube (TM) screening pipeline and found that Protein Kinase C activators disrupt TMs, silence Ca²⁺ “pacemakers” and boost radiotherapy.
doi.org/10.1158/2159...
In our paper, online @Cancer Discovery, we built an anti-tumor microtube (TM) screening pipeline and found that Protein Kinase C activators disrupt TMs, silence Ca²⁺ “pacemakers” and boost radiotherapy.
doi.org/10.1158/2159...
October 9, 2025 at 8:52 PM
How to disconnect therapy-resistant tumor networks in glioblastoma?
In our paper, online @Cancer Discovery, we built an anti-tumor microtube (TM) screening pipeline and found that Protein Kinase C activators disrupt TMs, silence Ca²⁺ “pacemakers” and boost radiotherapy.
doi.org/10.1158/2159...
In our paper, online @Cancer Discovery, we built an anti-tumor microtube (TM) screening pipeline and found that Protein Kinase C activators disrupt TMs, silence Ca²⁺ “pacemakers” and boost radiotherapy.
doi.org/10.1158/2159...
We introduce a scalable anti-TM drug screening platform and reveal PKC activation as a TM master-regulator. TM targeting disconnects tumor networks and sensitizes GBM to therapy. #Translational #CancerNeuroscience #glioblastoma
October 9, 2025 at 8:31 PM
We introduce a scalable anti-TM drug screening platform and reveal PKC activation as a TM master-regulator. TM targeting disconnects tumor networks and sensitizes GBM to therapy. #Translational #CancerNeuroscience #glioblastoma
Spatial multi-omics analysis (with COMET) of brains revealed that cells in the TPPB/RT combination treatment group exhibited fewer and shorter TMs, reduced proliferation, increased cell death, and decreased TTYH1 expression (a driver of TM invasion)
#GBM #COMET #Lunaphore @bio-techne.bsky.social
#GBM #COMET #Lunaphore @bio-techne.bsky.social
October 9, 2025 at 8:31 PM
Spatial multi-omics analysis (with COMET) of brains revealed that cells in the TPPB/RT combination treatment group exhibited fewer and shorter TMs, reduced proliferation, increased cell death, and decreased TTYH1 expression (a driver of TM invasion)
#GBM #COMET #Lunaphore @bio-techne.bsky.social
#GBM #COMET #Lunaphore @bio-techne.bsky.social
Intravital 2-photon microscopy with RT: TPPB breaks tumor networks and boosts radiotherapy in mice. Control+RT → network persists. TPPB+RT → fewer tumor microtubes, network collapse, regional regression. #Radiotherapy #IntravitalMicroscopy
October 9, 2025 at 8:31 PM
Intravital 2-photon microscopy with RT: TPPB breaks tumor networks and boosts radiotherapy in mice. Control+RT → network persists. TPPB+RT → fewer tumor microtubes, network collapse, regional regression. #Radiotherapy #IntravitalMicroscopy
The efficacy of TPPB was substantiated in 3D human glioma cortical spheroids and corroborated by longitudinal intravital imaging of mouse brains and histological analysis. Precisely, application of TPPB in vivo with radiotherapy reduced tumor burden and conferred a survival benefit vs RT alone.
October 9, 2025 at 8:31 PM
The efficacy of TPPB was substantiated in 3D human glioma cortical spheroids and corroborated by longitudinal intravital imaging of mouse brains and histological analysis. Precisely, application of TPPB in vivo with radiotherapy reduced tumor burden and conferred a survival benefit vs RT alone.
Likewise, they inhibitied global Ca²⁺ activity and pacemaker-like cells fell silent. #PacemakerCell #calciumimaging #periodicity
October 9, 2025 at 8:31 PM
Likewise, they inhibitied global Ca²⁺ activity and pacemaker-like cells fell silent. #PacemakerCell #calciumimaging #periodicity
PKC modulators emerged as the lead class of anti-TM drugs. Their members TPPB & PMA reduced TMs per cell and TM length without overt cytotoxicity. #PKC #TMs #cancerneuroscience #cancer
October 9, 2025 at 8:31 PM
PKC modulators emerged as the lead class of anti-TM drugs. Their members TPPB & PMA reduced TMs per cell and TM length without overt cytotoxicity. #PKC #TMs #cancerneuroscience #cancer
We built a machine-learning based 2D/3D morphometry pipeline to quantify TMs and network activity in 2D GB stem-like cells, 3D human brain spheroids (hGliCS), and mice. Using this platform, we screened 71 compounds to identify the most potent ones. #TumorMicrotubes
October 9, 2025 at 8:31 PM
We built a machine-learning based 2D/3D morphometry pipeline to quantify TMs and network activity in 2D GB stem-like cells, 3D human brain spheroids (hGliCS), and mice. Using this platform, we screened 71 compounds to identify the most potent ones. #TumorMicrotubes
Glioblastoma cells extend TMs to invade and wire into resilient networks. Particularly these network-integrated cells communicate via Ca²⁺waves and resist standard therapy. Thus, targeting TMs holds promise to overcome the resistance. #Glioblastoma #CancerNeuroscience
October 9, 2025 at 8:31 PM
Glioblastoma cells extend TMs to invade and wire into resilient networks. Particularly these network-integrated cells communicate via Ca²⁺waves and resist standard therapy. Thus, targeting TMs holds promise to overcome the resistance. #Glioblastoma #CancerNeuroscience
Frank Winkler will be presenting, amongst many other amazing speakers, at the free ✨Nature Conference✨ on translational cancer research.
Join in and meet fellow researchers from various disciplines to discuss new insights!
Abstract submissions: go.nature.com/4dECvak
Join in and meet fellow researchers from various disciplines to discuss new insights!
Abstract submissions: go.nature.com/4dECvak
June 12, 2025 at 5:05 AM
Frank Winkler will be presenting, amongst many other amazing speakers, at the free ✨Nature Conference✨ on translational cancer research.
Join in and meet fellow researchers from various disciplines to discuss new insights!
Abstract submissions: go.nature.com/4dECvak
Join in and meet fellow researchers from various disciplines to discuss new insights!
Abstract submissions: go.nature.com/4dECvak
#aufstehenfuerdemokratie
Um 11:55 Uhr versammelte sich eine große Gruppe von Wissenschaftlern & Unterstützern, um ein Statement für faktenbasierte Entscheidungen und Demokratie abzugeben:
Geht wahlen-mit Verstand!
Inspirierende Worte von Prof. E. Winkler und Prof. S. Pfister -
Danke an Alle!
Um 11:55 Uhr versammelte sich eine große Gruppe von Wissenschaftlern & Unterstützern, um ein Statement für faktenbasierte Entscheidungen und Demokratie abzugeben:
Geht wahlen-mit Verstand!
Inspirierende Worte von Prof. E. Winkler und Prof. S. Pfister -
Danke an Alle!
February 12, 2025 at 11:22 AM
#aufstehenfuerdemokratie
Um 11:55 Uhr versammelte sich eine große Gruppe von Wissenschaftlern & Unterstützern, um ein Statement für faktenbasierte Entscheidungen und Demokratie abzugeben:
Geht wahlen-mit Verstand!
Inspirierende Worte von Prof. E. Winkler und Prof. S. Pfister -
Danke an Alle!
Um 11:55 Uhr versammelte sich eine große Gruppe von Wissenschaftlern & Unterstützern, um ein Statement für faktenbasierte Entscheidungen und Demokratie abzugeben:
Geht wahlen-mit Verstand!
Inspirierende Worte von Prof. E. Winkler und Prof. S. Pfister -
Danke an Alle!
📢 Es ist 5 vor 12❗
"Aufstehen für Demokratie" setzt sich für faktenbasierte politische Entscheidungen und Demokratie ein:
www.aufstehenfuerdemokratie.de
www.openpetition.de/!ychtb
📅 Sei dabei am 12.02 um 11:55 Uhr an Hochschulen und Forschungseinrichtungen
🔬 Wissenschaft braucht Demokratie 🤝
"Aufstehen für Demokratie" setzt sich für faktenbasierte politische Entscheidungen und Demokratie ein:
www.aufstehenfuerdemokratie.de
www.openpetition.de/!ychtb
📅 Sei dabei am 12.02 um 11:55 Uhr an Hochschulen und Forschungseinrichtungen
🔬 Wissenschaft braucht Demokratie 🤝
February 7, 2025 at 4:53 PM
📢 Es ist 5 vor 12❗
"Aufstehen für Demokratie" setzt sich für faktenbasierte politische Entscheidungen und Demokratie ein:
www.aufstehenfuerdemokratie.de
www.openpetition.de/!ychtb
📅 Sei dabei am 12.02 um 11:55 Uhr an Hochschulen und Forschungseinrichtungen
🔬 Wissenschaft braucht Demokratie 🤝
"Aufstehen für Demokratie" setzt sich für faktenbasierte politische Entscheidungen und Demokratie ein:
www.aufstehenfuerdemokratie.de
www.openpetition.de/!ychtb
📅 Sei dabei am 12.02 um 11:55 Uhr an Hochschulen und Forschungseinrichtungen
🔬 Wissenschaft braucht Demokratie 🤝
📢 It is 5 min before 12❗
"Aufstehen für Demokratie" is committed to fact-based political decisions & democratic processes:
www.aufstehenfuerdemokratie.de
www.openpetition.de/!ychtb
📅 Join us on Feb 12th at 11:55 am at universities and research institutions in Germany.
🔬 Science needs democracy 🤝
"Aufstehen für Demokratie" is committed to fact-based political decisions & democratic processes:
www.aufstehenfuerdemokratie.de
www.openpetition.de/!ychtb
📅 Join us on Feb 12th at 11:55 am at universities and research institutions in Germany.
🔬 Science needs democracy 🤝
February 7, 2025 at 4:50 PM
📢 It is 5 min before 12❗
"Aufstehen für Demokratie" is committed to fact-based political decisions & democratic processes:
www.aufstehenfuerdemokratie.de
www.openpetition.de/!ychtb
📅 Join us on Feb 12th at 11:55 am at universities and research institutions in Germany.
🔬 Science needs democracy 🤝
"Aufstehen für Demokratie" is committed to fact-based political decisions & democratic processes:
www.aufstehenfuerdemokratie.de
www.openpetition.de/!ychtb
📅 Join us on Feb 12th at 11:55 am at universities and research institutions in Germany.
🔬 Science needs democracy 🤝
SAVE THE DATE
Discuss the interactions between the nervous system and cancer in our first @helloeacr.bsky.social conference on #CancerNeuroscience next year!
🗓️ Oct. 14-16, 2025
📍Bilbao, Spain
📥 Abstracts due June 30, 2025
📥 Register by Sept. 15, 2025
For more info, visit eacr.org/conference/c...
Discuss the interactions between the nervous system and cancer in our first @helloeacr.bsky.social conference on #CancerNeuroscience next year!
🗓️ Oct. 14-16, 2025
📍Bilbao, Spain
📥 Abstracts due June 30, 2025
📥 Register by Sept. 15, 2025
For more info, visit eacr.org/conference/c...
November 29, 2024 at 10:10 AM
SAVE THE DATE
Discuss the interactions between the nervous system and cancer in our first @helloeacr.bsky.social conference on #CancerNeuroscience next year!
🗓️ Oct. 14-16, 2025
📍Bilbao, Spain
📥 Abstracts due June 30, 2025
📥 Register by Sept. 15, 2025
For more info, visit eacr.org/conference/c...
Discuss the interactions between the nervous system and cancer in our first @helloeacr.bsky.social conference on #CancerNeuroscience next year!
🗓️ Oct. 14-16, 2025
📍Bilbao, Spain
📥 Abstracts due June 30, 2025
📥 Register by Sept. 15, 2025
For more info, visit eacr.org/conference/c...
Very happy to be at #SNO2024!
Representing the Winkler Lab are Frank Winkler, Niels Olshausen @nielsolshausen.bsky.social and Nils Hebach @nilshebach.bsky.social !
Looking forward to meeting many friends here 🧠🦀🔬
@neuroonc.bsky.social #cancerneuroscience
Representing the Winkler Lab are Frank Winkler, Niels Olshausen @nielsolshausen.bsky.social and Nils Hebach @nilshebach.bsky.social !
Looking forward to meeting many friends here 🧠🦀🔬
@neuroonc.bsky.social #cancerneuroscience
November 23, 2024 at 6:49 PM
Very happy to be at #SNO2024!
Representing the Winkler Lab are Frank Winkler, Niels Olshausen @nielsolshausen.bsky.social and Nils Hebach @nilshebach.bsky.social !
Looking forward to meeting many friends here 🧠🦀🔬
@neuroonc.bsky.social #cancerneuroscience
Representing the Winkler Lab are Frank Winkler, Niels Olshausen @nielsolshausen.bsky.social and Nils Hebach @nilshebach.bsky.social !
Looking forward to meeting many friends here 🧠🦀🔬
@neuroonc.bsky.social #cancerneuroscience
📢 We are excited for the #EACR2025 meeting in beautiful Lisbon!
📅 June 16-19 2025
Please join us for the
Tumor Innervation Symposium #cancerneuroscience
and many more exciting sessions!
Abstract submission open: Dec 2nd
👩💻https://2025.eacr.org/abstracts
📅 June 16-19 2025
Please join us for the
Tumor Innervation Symposium #cancerneuroscience
and many more exciting sessions!
Abstract submission open: Dec 2nd
👩💻https://2025.eacr.org/abstracts
November 21, 2024 at 8:12 AM
📢 We are excited for the #EACR2025 meeting in beautiful Lisbon!
📅 June 16-19 2025
Please join us for the
Tumor Innervation Symposium #cancerneuroscience
and many more exciting sessions!
Abstract submission open: Dec 2nd
👩💻https://2025.eacr.org/abstracts
📅 June 16-19 2025
Please join us for the
Tumor Innervation Symposium #cancerneuroscience
and many more exciting sessions!
Abstract submission open: Dec 2nd
👩💻https://2025.eacr.org/abstracts