link to the article to avoid 10000 cookies: elifesciences.org/reviewed-pre...

hahaha this is amazing!!!

this project has been such a joy, and collaborating with dalofa.bsky.social, @thombooth.bsky.social and @tilmweber.bsky.social had been fantastic, it has been everything I ever dreamed an academic collaboration could be. Thank you guys!

...an information which David then used to beautifully show that the protein folds similarly in structure but not in sequence to the known telomere maintenance systems main DNAbinding domain.

Then @thombooth.bsky.social used the presence/absence of known telomere proteins to identify a potentially new telomere protein which is linked to the Sg2247 class telomere, which previously did not have an identified maintenance system (notice the dot in the red circle)

David then analyzed the end replication proteins and could identify a known system in 76% of strains. Extremely interestingly, he used that to show that certain telomere sequences are linked to specific telomere maintenance proteins.

To our knowledge, the >2000 telomeres which were clustered into 137 groups is the first large scale attempt at dissecting the diversity of the telomeres of streptomyces, and allow us to make additional discoveries, like the potentially #plasmid specific AGA telomere.

We noticed that quite few of the RefSeq genomes had replicon ends which clustered as telomeres, and decided to quantify it: while only 15% of RefSeq 'complete' chromosomes were found to have both telomeres, 78% of Telomore completed chromosomes had both telomeres.

After figuring out how to attach the missing telomeres, we went on to extract replicon ends from #RefSeq to make a compendium of >2000 streptomycetaceae telomeres, clustered by sequence similarity into 137 groups with 4-300+ members.

The tool, Telomore, works by mapping first long then short reads to linear replicon ends, then building a consensus sequence from the reads overhanging the end, then attaching the consensus to create a complete sequence with no gaps.