...an information which David then used to beautifully show that the protein folds similarly in structure but not in sequence to the known telomere maintenance systems main DNAbinding domain.

Then @thombooth.bsky.social used the presence/absence of known telomere proteins to identify a potentially new telomere protein which is linked to the Sg2247 class telomere, which previously did not have an identified maintenance system (notice the dot in the red circle)

David then analyzed the end replication proteins and could identify a known system in 76% of strains. Extremely interestingly, he used that to show that certain telomere sequences are linked to specific telomere maintenance proteins.

To our knowledge, the >2000 telomeres which were clustered into 137 groups is the first large scale attempt at dissecting the diversity of the telomeres of streptomyces, and allow us to make additional discoveries, like the potentially #plasmid specific AGA telomere.

We noticed that quite few of the RefSeq genomes had replicon ends which clustered as telomeres, and decided to quantify it: while only 15% of RefSeq 'complete' chromosomes were found to have both telomeres, 78% of Telomore completed chromosomes had both telomeres.

The tool, Telomore, works by mapping first long then short reads to linear replicon ends, then building a consensus sequence from the reads overhanging the end, then attaching the consensus to create a complete sequence with no gaps.

Bacterial telomeres are common, just not so much in RefSeq 'complete' genomes. But they can be added by the new tool David Faurdal wrote. I am thrilled to see this out as a preprint here: www.biorxiv.org/content/10.1... @tilmweber.bsky.social @thombooth.bsky.social

update: same flowcell with the same library moved to an old MinION Mk1B works beautifully 🧬🖥️🧪

Has anyone seen this pattern before? @nanoporetech.com 10.4.1 flowcell (severely over warrenty..) on new #Mk1D. Pores get zapped at the first mux scan, and the remaining pores sequence at 200bps instead of 400bps, despite target temp? (also how can there be more failed reads than total reads?)

New pangenome of Streptomyces study out! I found the BGC synteny within species particularly interesting as it changes my view of what I thought was hybermobile chromosome ends: they don't seem to be changing that much or fast, mostly just certain groups picking up a few BGCs here and there.

new lab member and a bunch of workhorses (on their makeshift heat sink) @nanoporetech.com

They are copies of whatever was on the other arm at the time when a breakhappened, often they carry BGCs, sometimes half BGCs if the event happened in the middle of a gene cluster, and they are packed with transposases. explained beautifully here: doi.org/10.1038/s415...

New manuscript is out on a scale new to me: We sequenced >1000 genomes, mostly complete, of filamentous Actinobacteria. With these, we can start asking questions like "How long are Streptomyces terminal inverted repeats" and get answers like this 🧬🖥️ #secmet.