Tien-Chi Huang
@tienchihuang.bsky.social
Interested in chromatin dynamics during mammalian development
Pinned
Happy to share our latest preprint.
We uncover a previously uncharacterised and evolutionarily conserved chromatin architecture in premeiotic gonadal germ cells, revealed through integrated cytological and high-resolution Hi-C approaches.
www.biorxiv.org/content/10.1...
Thread🧵🔽
We uncover a previously uncharacterised and evolutionarily conserved chromatin architecture in premeiotic gonadal germ cells, revealed through integrated cytological and high-resolution Hi-C approaches.
www.biorxiv.org/content/10.1...
Thread🧵🔽
Happy to share our latest preprint.
We uncover a previously uncharacterised and evolutionarily conserved chromatin architecture in premeiotic gonadal germ cells, revealed through integrated cytological and high-resolution Hi-C approaches.
www.biorxiv.org/content/10.1...
Thread🧵🔽
We uncover a previously uncharacterised and evolutionarily conserved chromatin architecture in premeiotic gonadal germ cells, revealed through integrated cytological and high-resolution Hi-C approaches.
www.biorxiv.org/content/10.1...
Thread🧵🔽
November 18, 2025 at 4:57 PM
Happy to share our latest preprint.
We uncover a previously uncharacterised and evolutionarily conserved chromatin architecture in premeiotic gonadal germ cells, revealed through integrated cytological and high-resolution Hi-C approaches.
www.biorxiv.org/content/10.1...
Thread🧵🔽
We uncover a previously uncharacterised and evolutionarily conserved chromatin architecture in premeiotic gonadal germ cells, revealed through integrated cytological and high-resolution Hi-C approaches.
www.biorxiv.org/content/10.1...
Thread🧵🔽
Reposted by Tien-Chi Huang
[3] Navigating the pitfalls of mapping DNA and RNA modifications www.nature.com/articles/s41... [*] Please note the detection of exogeneous DNA mods is different from mapping natural endogenous DNA mods , because abundant exogeneous DNA mods are introduced (e.g. Fiber-seq, DiMeLo-seq, etc) n/n [END]
Navigating the pitfalls of mapping DNA and RNA modifications - Nature Reviews Genetics
The ability to map DNA and RNA modifications has improved our understanding of these marks, but in some cases inconsistent results have been problematic. Here, Kong et al. discuss how to recognize and...
www.nature.com
November 20, 2024 at 11:41 AM
[3] Navigating the pitfalls of mapping DNA and RNA modifications www.nature.com/articles/s41... [*] Please note the detection of exogeneous DNA mods is different from mapping natural endogenous DNA mods , because abundant exogeneous DNA mods are introduced (e.g. Fiber-seq, DiMeLo-seq, etc) n/n [END]
Reposted by Tien-Chi Huang
I believe that over the past ~ two decades, since the breakthrough papers on UHRF1, this is the first reported DNA methylation phenotype of its close paralog, UHRF2 (and not for lack of trying). Congrats to Ambre Bender and Michael Weber on this fantastic study! www.nature.com/articles/s41...
UHRF2 mediates resistance to DNA methylation reprogramming in primordial germ cells - Nature Communications
DNA methylation in mouse primordial germ cells (PGCs) is restricted to transposable elements, but how this unique DNA methylome is established is poorly understood. Here, the authors identify UHRF2 as...
www.nature.com
August 9, 2025 at 6:26 PM
I believe that over the past ~ two decades, since the breakthrough papers on UHRF1, this is the first reported DNA methylation phenotype of its close paralog, UHRF2 (and not for lack of trying). Congrats to Ambre Bender and Michael Weber on this fantastic study! www.nature.com/articles/s41...
Reading published results is like looking at the distant star-everything you see happened in the deep past. Light years between a truly novel discovery and its communication within the scientific community.
How it started / How it's going 😱
(www.biorxiv.org/content/10.1... / www.nature.com/articles/s41...)
#preprint
(www.biorxiv.org/content/10.1... / www.nature.com/articles/s41...)
#preprint
July 20, 2025 at 11:33 AM
Reading published results is like looking at the distant star-everything you see happened in the deep past. Light years between a truly novel discovery and its communication within the scientific community.
Reposted by Tien-Chi Huang
Finally out! 🥳 Our paper showing how a transposable element (TE) insertion can cause developmental phenotypes is now published @natgenet.nature.com 🧬🦠🐁
Below is a brief description of the major findings. Check the full version of the paper for more details: www.nature.com/articles/s41588-025-02248-5
Below is a brief description of the major findings. Check the full version of the paper for more details: www.nature.com/articles/s41588-025-02248-5
Enhancer adoption by an LTR retrotransposon generates viral-like particles, causing developmental limb phenotypes - Nature Genetics
Activation of an LTR retrotransposon inserted upstream of the Fgf8 gene produces viral-like particles in the mouse developing limb, triggering apoptosis and causing limb malformation. This phenotype c...
www.nature.com
July 9, 2025 at 10:05 AM
Finally out! 🥳 Our paper showing how a transposable element (TE) insertion can cause developmental phenotypes is now published @natgenet.nature.com 🧬🦠🐁
Below is a brief description of the major findings. Check the full version of the paper for more details: www.nature.com/articles/s41588-025-02248-5
Below is a brief description of the major findings. Check the full version of the paper for more details: www.nature.com/articles/s41588-025-02248-5
It was nice to hear this story in the GRC conference. Glad it’s out.
Generation of germinal-vesicle oocytes from mouse embryonic stem cells under an ovarian soma-free condition
Using mouse pluripotent stem cells, Nosaka et al. created an ovarian somatic cell
(OSC)-free system to induce an abundant number of oocyte-like cells at diplotene arrest,
followed by their growth into...
www.cell.com
July 4, 2025 at 3:17 PM
It was nice to hear this story in the GRC conference. Glad it’s out.
Reposted by Tien-Chi Huang
🚨 Preprint alert 🚨
Excited to share our work on "Ab-trapping," an antibody artifact causing misleading peripheral ("rim") staining in imaging & genomics (IF, CUT&Tag, CUT&RUN). Antibodies fail to penetrate structures, accumulating at the periphery. A 🧵👇
doi.org/10.1101/2025...
Excited to share our work on "Ab-trapping," an antibody artifact causing misleading peripheral ("rim") staining in imaging & genomics (IF, CUT&Tag, CUT&RUN). Antibodies fail to penetrate structures, accumulating at the periphery. A 🧵👇
doi.org/10.1101/2025...
Ab-trapping - a peripheral staining artifact in antibody-based microscopy and genomics
Antibodies (Ab) are essential for detecting specific epitopes in microscopy and genomics, but can produce artifacts leading to erroneous interpretations. Here, we characterize a novel artifact, Ab-tra...
doi.org
April 14, 2025 at 8:08 PM
🚨 Preprint alert 🚨
Excited to share our work on "Ab-trapping," an antibody artifact causing misleading peripheral ("rim") staining in imaging & genomics (IF, CUT&Tag, CUT&RUN). Antibodies fail to penetrate structures, accumulating at the periphery. A 🧵👇
doi.org/10.1101/2025...
Excited to share our work on "Ab-trapping," an antibody artifact causing misleading peripheral ("rim") staining in imaging & genomics (IF, CUT&Tag, CUT&RUN). Antibodies fail to penetrate structures, accumulating at the periphery. A 🧵👇
doi.org/10.1101/2025...
Reposted by Tien-Chi Huang
Paper out !!🥳big thanks to all authors @marliesoomen.bsky.social @diego-rt.bsky.social @kaessmannlab.bsky.social @jonathangoeke.bsky.social @lorenzamottes.bsky.social & 'bluesky-less'
Lots of interesting new TE (& genes) biology
Data fully browsable💻 👉 embryo.helmholtz-munich.de/shiny_embryo/
Lots of interesting new TE (& genes) biology
Data fully browsable💻 👉 embryo.helmholtz-munich.de/shiny_embryo/
February 21, 2025 at 3:50 PM
Paper out !!🥳big thanks to all authors @marliesoomen.bsky.social @diego-rt.bsky.social @kaessmannlab.bsky.social @jonathangoeke.bsky.social @lorenzamottes.bsky.social & 'bluesky-less'
Lots of interesting new TE (& genes) biology
Data fully browsable💻 👉 embryo.helmholtz-munich.de/shiny_embryo/
Lots of interesting new TE (& genes) biology
Data fully browsable💻 👉 embryo.helmholtz-munich.de/shiny_embryo/
Reposted by Tien-Chi Huang
There has been a couple of manuscripts released regarding transcription factor footprinting - or digital genomic footprinting (DGF) as we call it. I would like to put some of the claims, results and conclusions in context, since I have worked and published on this for ~15 years. 1/n
January 24, 2025 at 10:54 PM
There has been a couple of manuscripts released regarding transcription factor footprinting - or digital genomic footprinting (DGF) as we call it. I would like to put some of the claims, results and conclusions in context, since I have worked and published on this for ~15 years. 1/n
Reposted by Tien-Chi Huang
Microscopy Nodes is now up on bioRxiv! 🚀
This is a Blender extension that seamlessly integrates and visualizes 3D microscopy data (TIF & @zarr.dev).
High-quality volume rendering for anyone, in both EM and fluorescence, regardless of computational expertise! 🔬
www.biorxiv.org/content/10.1...
This is a Blender extension that seamlessly integrates and visualizes 3D microscopy data (TIF & @zarr.dev).
High-quality volume rendering for anyone, in both EM and fluorescence, regardless of computational expertise! 🔬
www.biorxiv.org/content/10.1...
January 15, 2025 at 1:39 PM
Microscopy Nodes is now up on bioRxiv! 🚀
This is a Blender extension that seamlessly integrates and visualizes 3D microscopy data (TIF & @zarr.dev).
High-quality volume rendering for anyone, in both EM and fluorescence, regardless of computational expertise! 🔬
www.biorxiv.org/content/10.1...
This is a Blender extension that seamlessly integrates and visualizes 3D microscopy data (TIF & @zarr.dev).
High-quality volume rendering for anyone, in both EM and fluorescence, regardless of computational expertise! 🔬
www.biorxiv.org/content/10.1...