Randi Guest
@randna.bsky.social
Assistant Professor at MacEwan University | Bacterial Envelope Enthusiast | She/Her
A chimeric Mla-Pqi lipid transport system is required for Brucella abortus survival in macrophages
www.embopress.org/doi/full/10....
www.embopress.org/doi/full/10....
A chimeric Mla-Pqi lipid transport system is required for Brucella abortus survival in macrophages | The EMBO Journal
imageimageSeveral systems, such as the Mla and Pqi pathways, mediate lipid transport between
the two membranes of gram-negative bacteria. This study identifies a novel Mla-Pqi
chimeric complex in Bruc...
www.embopress.org
August 9, 2025 at 8:28 PM
A chimeric Mla-Pqi lipid transport system is required for Brucella abortus survival in macrophages
www.embopress.org/doi/full/10....
www.embopress.org/doi/full/10....
Reposted by Randi Guest
#NewResearch
Outer membrane attachment to peptidoglycan enables periplasmic pressure to build up and oppose cytoplasmic turgor pressure, preventing lysis during osmotic challenge in Gram-negative bacteria
@michaeldeghelt.bsky.social #MicroSky 🦠
www.nature.com/articles/s41...
Outer membrane attachment to peptidoglycan enables periplasmic pressure to build up and oppose cytoplasmic turgor pressure, preventing lysis during osmotic challenge in Gram-negative bacteria
@michaeldeghelt.bsky.social #MicroSky 🦠
www.nature.com/articles/s41...
Peptidoglycan–outer membrane attachment generates periplasmic pressure to prevent lysis in Gram-negative bacteria - Nature Microbiology
Outer membrane attachment to peptidoglycan enables periplasmic pressure to build up and counter cytoplasmic turgor pressure, preventing lysis during osmotic challenges in Escherichia coli.
www.nature.com
July 30, 2025 at 3:24 PM
#NewResearch
Outer membrane attachment to peptidoglycan enables periplasmic pressure to build up and oppose cytoplasmic turgor pressure, preventing lysis during osmotic challenge in Gram-negative bacteria
@michaeldeghelt.bsky.social #MicroSky 🦠
www.nature.com/articles/s41...
Outer membrane attachment to peptidoglycan enables periplasmic pressure to build up and oppose cytoplasmic turgor pressure, preventing lysis during osmotic challenge in Gram-negative bacteria
@michaeldeghelt.bsky.social #MicroSky 🦠
www.nature.com/articles/s41...
Reposted by Randi Guest
A recent paper reveals that while LPS deficiency shields bacteria from phage infection (they lack receptor), it heightens vulnerability to T6SS contact-dependent antagonism, highlighting a critical evolutionary constraint.
by @seeyeunting.bsky.social
www.embopress.org/doi/full/10....
by @seeyeunting.bsky.social
www.embopress.org/doi/full/10....
www.embopress.org
July 24, 2025 at 8:49 AM
A recent paper reveals that while LPS deficiency shields bacteria from phage infection (they lack receptor), it heightens vulnerability to T6SS contact-dependent antagonism, highlighting a critical evolutionary constraint.
by @seeyeunting.bsky.social
www.embopress.org/doi/full/10....
by @seeyeunting.bsky.social
www.embopress.org/doi/full/10....
Reposted by Randi Guest
just like among humans, 𝗺𝗼𝗯bing is rampant in the microbial world.
here 👇 Maria Pilar Garcillán-Barcia , Fernando de la Cruz, and Eduardo Rocha review the extended 𝗺𝗼𝗯ility of plasmids...
#MicroSky
here 👇 Maria Pilar Garcillán-Barcia , Fernando de la Cruz, and Eduardo Rocha review the extended 𝗺𝗼𝗯ility of plasmids...
#MicroSky
Here's our new broad review on the extended mobility of plasmids, about all mechanisms driving and limiting their transfer. From conjugation to conduction, phage-plasmids to hitchers, molecular to evolutionary dynamics, ecology to biotech. The state of affairs. 1/9 academic.oup.com/nar/article/...
July 23, 2025 at 8:54 AM
just like among humans, 𝗺𝗼𝗯bing is rampant in the microbial world.
here 👇 Maria Pilar Garcillán-Barcia , Fernando de la Cruz, and Eduardo Rocha review the extended 𝗺𝗼𝗯ility of plasmids...
#MicroSky
here 👇 Maria Pilar Garcillán-Barcia , Fernando de la Cruz, and Eduardo Rocha review the extended 𝗺𝗼𝗯ility of plasmids...
#MicroSky
Reposted by Randi Guest
Cool result: Transposon insertion sequencing reveals novel hypermutator genes in Acinetobacter baumannii
"Exposure of a transposon insertion mutant library to extended weak antibiotic selection ... revealed genes that indirectly increased fitness due to elevated general mutation rates"
"Exposure of a transposon insertion mutant library to extended weak antibiotic selection ... revealed genes that indirectly increased fitness due to elevated general mutation rates"
Transposon insertion sequencing reveals novel hypermutator genes in Acinetobacter baumannii | mBio
All organisms have the capacity for evolution through mutation. Bacteria with high
mutation rates have a survival advantage in some stressful environments because they
generate beneficial mutations mo...
journals.asm.org
June 29, 2025 at 8:44 AM
Reposted by Randi Guest
Just as our bodies have evolved to defend us against bacterial and viral pathogens, bacteria face the same challenges. They must deter their own viruses, chemically compete with other microbes, and deal with challenges from eukaryotic hosts 1/n 🧪
June 29, 2025 at 12:53 PM
Just as our bodies have evolved to defend us against bacterial and viral pathogens, bacteria face the same challenges. They must deter their own viruses, chemically compete with other microbes, and deal with challenges from eukaryotic hosts 1/n 🧪
Reposted by Randi Guest
weekend is here! comes with a most welcome recording on youtube 👇
and if you need a less crick-heavy read, from the same author: DOI: 10.1016/j.cub.2015.05.032
#MicroSky
and if you need a less crick-heavy read, from the same author: DOI: 10.1016/j.cub.2015.05.032
#MicroSky
June 20, 2025 at 12:44 PM
weekend is here! comes with a most welcome recording on youtube 👇
and if you need a less crick-heavy read, from the same author: DOI: 10.1016/j.cub.2015.05.032
#MicroSky
and if you need a less crick-heavy read, from the same author: DOI: 10.1016/j.cub.2015.05.032
#MicroSky
Reposted by Randi Guest
Molecular interplay between peptidoglycan integrity and outer membrane asymmetry in maintaining cell envelope homeostasis https://www.biorxiv.org/content/10.1101/2025.06.19.660527v1
June 20, 2025 at 3:16 AM
Molecular interplay between peptidoglycan integrity and outer membrane asymmetry in maintaining cell envelope homeostasis https://www.biorxiv.org/content/10.1101/2025.06.19.660527v1
Reposted by Randi Guest
#MicrobiologyMonday: Pseudomonas aeruginosa releases a surfactant that displaces Klebsiella pneumoniae from solid surfaces. By pushing K. pneumoniae away, the detergent allows P. aeruginosa to better compete for limited iron. Learn more in #mBio: asm.social/2sH
June 16, 2025 at 5:57 PM
#MicrobiologyMonday: Pseudomonas aeruginosa releases a surfactant that displaces Klebsiella pneumoniae from solid surfaces. By pushing K. pneumoniae away, the detergent allows P. aeruginosa to better compete for limited iron. Learn more in #mBio: asm.social/2sH
Reposted by Randi Guest
Just in case you missed it, watch our video ‘What is AMR?’, which explains the fundamentals of Antimicrobial Resistance and the strategies and solutions to mitigate its threat. Watch the full video now -https://youtu.be/hXr2Rn9OTOg #KnockingOutAMR
What is Antimicrobial Resistance and how can we tackle it?
Captions are available in many other languages: Please press the settings button (gear icon) for more options. Antimicrobial resistance (AMR) is one of most ...
youtu.be
June 17, 2025 at 9:02 AM
Just in case you missed it, watch our video ‘What is AMR?’, which explains the fundamentals of Antimicrobial Resistance and the strategies and solutions to mitigate its threat. Watch the full video now -https://youtu.be/hXr2Rn9OTOg #KnockingOutAMR
Reposted by Randi Guest
Very happy to share that a large part of my thesis work is out today: B. subtilis uses the second messenger c-di-AMP to modulate its turgor pressure in response to the state of its cell envelope. www.nature.com/articles/s41...
Cyclic-di-AMP modulates cellular turgor in response to defects in bacterial cell wall synthesis - Nature Microbiology
Brogan et al. uncover a signalling pathway in which levels of the nucleotide second messenger c-di-AMP increase in response to defects in cell wall synthesis. This regulatory pathway decreases turgor ...
www.nature.com
June 17, 2025 at 12:20 PM
Very happy to share that a large part of my thesis work is out today: B. subtilis uses the second messenger c-di-AMP to modulate its turgor pressure in response to the state of its cell envelope. www.nature.com/articles/s41...
Reposted by Randi Guest
A connection between two ancient and essential cellular processes, iron-sulfur protein biogenesis and fatty acid synthesis, in Escherichia coli https://www.biorxiv.org/content/10.1101/2025.06.16.659903v1
June 17, 2025 at 3:18 AM
A connection between two ancient and essential cellular processes, iron-sulfur protein biogenesis and fatty acid synthesis, in Escherichia coli https://www.biorxiv.org/content/10.1101/2025.06.16.659903v1
Reposted by Randi Guest
Impaired envelope integrity in the absence of SanA is linked to increased Lipid II availability and an imbalance of FtsI and FtsW activities https://www.biorxiv.org/content/10.1101/2025.06.10.658892v1
June 11, 2025 at 3:17 AM
Impaired envelope integrity in the absence of SanA is linked to increased Lipid II availability and an imbalance of FtsI and FtsW activities https://www.biorxiv.org/content/10.1101/2025.06.10.658892v1
Reposted by Randi Guest
February 18, 2025 at 8:03 AM
Reposted by Randi Guest
Latest paper drop from Yale Phage Center @paulturnerlab.bsky.social
Yale researchers have made a breakthrough in fighting drug-resistant infections in cystic fibrosis with inhaled bacteriophage therapy - offering new hope against antimicrobial resistance.
🔗 www.nature.com/articles/s41...
🔗 www.nature.com/articles/s41...
Personalized inhaled bacteriophage therapy for treatment of multidrug-resistant Pseudomonas aeruginosa in cystic fibrosis - Nature Medicine
A novel personalized phage therapy strategy that selects phages for a predicted evolutionary trade-off may represent a viable alternative approach for the treatment of antimicrobial-resistant bacteria...
www.nature.com
May 8, 2025 at 2:04 AM
Latest paper drop from Yale Phage Center @paulturnerlab.bsky.social
Reposted by Randi Guest
It's Thursday: Episode 89 of #MattersMicrobial is up, with Dr. Amir Mitchell of University of Masschusetts Medical School! Today, the #QualityQuorum discussed using AI to identify new antibiotics. Please spread the #GoodMicrobialWord? @profvrr
youtu.be/Wa0zd-TRCO8?...
youtu.be/Wa0zd-TRCO8?...
May 1, 2025 at 10:55 PM
It's Thursday: Episode 89 of #MattersMicrobial is up, with Dr. Amir Mitchell of University of Masschusetts Medical School! Today, the #QualityQuorum discussed using AI to identify new antibiotics. Please spread the #GoodMicrobialWord? @profvrr
youtu.be/Wa0zd-TRCO8?...
youtu.be/Wa0zd-TRCO8?...
Reposted by Randi Guest
"That telomere phages are so prevalent means that they are a selective force, one that we know little about. We now want to understand how the telomere-toxin is secreted and also understand how this ‘telocin’ wheedles its way into unsuspecting bacterial neighbors”
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Telomere bacteriophages are widespread and equip their bacterial hosts with potent interbacterial weapons
Klebsiella host strains infected with telomere phages can grow to be the dominant lineage in mixed populations.
www.science.org
May 1, 2025 at 10:04 PM
"That telomere phages are so prevalent means that they are a selective force, one that we know little about. We now want to understand how the telomere-toxin is secreted and also understand how this ‘telocin’ wheedles its way into unsuspecting bacterial neighbors”
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Randi Guest
Groundbreaking study in Cell from @leventallab.bsky.social: phospholipid asymmetry is a defining feature of the plasma membrane and cholesterol fills the holes — major implications for how this membrane works. A #lipidtime must-read! www.cell.com/cell/abstrac...
Cell membranes sustain phospholipid imbalance via cholesterol asymmetry
This work challenges a major assumption in cell biology by showing that lipid bilayers
can have drastically different phospholipid abundances between their two leaflets.
This lipid abundance asymmetry...
www.cell.com
April 3, 2025 at 3:07 PM
Groundbreaking study in Cell from @leventallab.bsky.social: phospholipid asymmetry is a defining feature of the plasma membrane and cholesterol fills the holes — major implications for how this membrane works. A #lipidtime must-read! www.cell.com/cell/abstrac...
Reposted by Randi Guest
📣 New MicroBites!
The Enemy of My Enemy is My Friend 🦠
How one bacteria befriended an old foe to take down a thriving competitor 👇
The Enemy of My Enemy is My Friend 🦠
How one bacteria befriended an old foe to take down a thriving competitor 👇
The Enemy of My Enemy is My Friend
How one bacteria befriended an old foe to take down a thriving competitor
micro-bites.org
April 14, 2025 at 11:43 AM
📣 New MicroBites!
The Enemy of My Enemy is My Friend 🦠
How one bacteria befriended an old foe to take down a thriving competitor 👇
The Enemy of My Enemy is My Friend 🦠
How one bacteria befriended an old foe to take down a thriving competitor 👇
Reposted by Randi Guest
Overlooked signals: Highly stable quorum sensing molecule in phage lysates induces quorum sensing response https://www.biorxiv.org/content/10.1101/2025.04.11.648453v1
April 12, 2025 at 4:18 AM
Overlooked signals: Highly stable quorum sensing molecule in phage lysates induces quorum sensing response https://www.biorxiv.org/content/10.1101/2025.04.11.648453v1
Reposted by Randi Guest
High-throughput LacZ/CPRG screen identifies novel potential antibiotics targeting Gram-negative bacterial envelopes to combat resistance https://www.biorxiv.org/content/10.1101/2025.04.09.648071v1
April 10, 2025 at 11:16 PM
High-throughput LacZ/CPRG screen identifies novel potential antibiotics targeting Gram-negative bacterial envelopes to combat resistance https://www.biorxiv.org/content/10.1101/2025.04.09.648071v1
Reposted by Randi Guest
Structural Basis of Lipopolysaccharide Assembly by the Outer Membrane Translocon Holo-Complex https://www.biorxiv.org/content/10.1101/2025.04.10.648201v1
April 11, 2025 at 3:16 AM
Structural Basis of Lipopolysaccharide Assembly by the Outer Membrane Translocon Holo-Complex https://www.biorxiv.org/content/10.1101/2025.04.10.648201v1
Reposted by Randi Guest
The bacterial HflK-HflC membrane complex plays a key role in cell #membrane organization. This study shows that HflK-HflC is important for aerobic #respiration, stabilizing proteins like IspG & TonB; reduced IspG activity impairs respiration & energy production @plosbiology.org 🧪 plos.io/3XPFENO
April 8, 2025 at 1:32 PM
The bacterial HflK-HflC membrane complex plays a key role in cell #membrane organization. This study shows that HflK-HflC is important for aerobic #respiration, stabilizing proteins like IspG & TonB; reduced IspG activity impairs respiration & energy production @plosbiology.org 🧪 plos.io/3XPFENO
Bacillus subtilis MurJ and Amj Lipid II flippases are not essential for growth
journals.asm.org/doi/10.1128/...
journals.asm.org/doi/10.1128/...
Bacillus subtilis MurJ and Amj Lipid II flippases are not essential for growth | Journal of Bacteriology
The assembly of peptidoglycan (PG), the typically essential polymer that provides
structural integrity to bacterial cells, begins with the synthesis of the Lipid II
monomer in the cytoplasm and along ...
journals.asm.org
April 7, 2025 at 7:04 PM
Bacillus subtilis MurJ and Amj Lipid II flippases are not essential for growth
journals.asm.org/doi/10.1128/...
journals.asm.org/doi/10.1128/...
Reposted by Randi Guest
Striking new study from @archaeon-alex.bsky.social's lab just out in @science.org on multicellular development induced by compression in Archaea: www.science.org/doi/10.1126/...
Tissue-like multicellular development triggered by mechanical compression in archaea
The advent of clonal multicellularity is a critical evolutionary milestone, seen often in eukaryotes, rarely in bacteria, and only once in archaea. We show that uniaxial compression induces clonal mul...
www.science.org
April 3, 2025 at 6:24 PM
Striking new study from @archaeon-alex.bsky.social's lab just out in @science.org on multicellular development induced by compression in Archaea: www.science.org/doi/10.1126/...