Roscoff (Bretagne), France, September 15-19, 2025Deadline for application: May 5, 2025

Abstract. We describe four ancient polyploidy events where the descendant taxa retain many more duplicated gene copies than has been seen in other paleopol

The plant, formally known as Ovicula biradiata, is especially notable for being the simultaneous discovery of a new species and genus. It was found with help from the community science app iNaturalist...

An exchange of comments probes the story of the EPAS2 gene, balancing selection, and resistance to Yersinia pestis.

Chris Stringer is tracing human ancestors back a million years, but pinpointing when exactly Homo sapiens emerged is even more complex

Wolves are among the earliest animals to be domesticated. However, the mechanism by which ancient wolves were domesticated into modern dogs is unknown. Most of the prevailing domestication hypotheses ...

Marking 100 years since Australopithecus Africanus transformed our understanding.

Taphonomic studies of osteoarchaeological human assemblages have mainly focused on establishing recognisable markers that allow us to discriminate between humanly induced modifications from natural ca...

Study highlights role of women in Celtic Britain and challenges assumptions most societies were patrilocal

The inversion of C3 stereochemistry in monoterpenoid indole alkaloids (MIAs), derived from the central precursor strictosidine (3S), is essential for synthesizing numerous 3R MIAs and oxindoles, including the antihypertensive drug reserpine found in Rauvolfia serpentina (Indian snakeroot) and Rauvolfia tetraphylla (devil pepper) of the plant family Apocynaceae. MIA biosynthesis begins with the reduction of strictosidine aglycone by various reductases, preserving the initial 3S stereochemistry. In this study, we identify and biochemically characterize a conserved oxidase-reductase pair from the Apocynaceae, Rubiaceae, and Gelsemiaceae families of the order Gentianales: the heteroyohimbine/yohimbine/corynanthe C3-oxidase (HYC3O) and C3-reductase (HYC3R). These enzymes collaboratively invert the 3S stereochemistry to 3R across a range of substrates, resolving the long-standing question about the origin of 3R MIAs and oxindole derivatives, and facilitation of reserpine biosynthesis. Notably, HYC3O and HYC3R are located within gene clusters in both the R. tetraphylla and Catharanthus roseus (Madagascar periwinkle) genomes, which are partially homologous to an elusive geissoschizine synthase (GS) gene cluster we also identified in these species. In R. tetraphylla, these clusters occur closely in tandem on a single chromosome, likely stemming from a single segmental duplication event, while in C. roseus, a closely related member of rauvolfioid Apocynaceae, they were later separated by a chromosomal translocation. The ancestral genomic context for both clusters can be traced all the way back to common ancestry with grapevine. Given the presence of syntenic GS homologs in Mitragyna speciosa (Rubiaceae), the GS cluster, at least in part, likely evolved at the base of the Gentianales, which split from other core eudicots up to 135 million years ago. We also show that the strictosidine biosynthetic gene cluster, required to initiate the MIA pathway, likely evolved concurrently. The reserpine biosynthetic cluster likely arose much later in the rauvolfioid lineage of Apocynaceae. Collectively, our work uncovers the genomic and biochemical basis for key events in MIA evolution and diversification, providing insights beyond the well-characterized vinblastine and ajmaline biosynthetic pathways. ### Competing Interest Statement The authors have declared no competing interest.

The skull provides some of the best evidence for the ancestral population of Neandertals, and had a tumultuous history in the decades after its discovery.

Researchers may have identified a new human species that lived around 300,000 years ago in Asia.

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