@microann.bsky.social
Reposted
Comparison of a long-read amplicon sequencing approach to short-read amplicons for microbiome analysis https://www.biorxiv.org/content/10.1101/2025.09.04.674341v1
September 6, 2025 at 3:17 AM
Comparison of a long-read amplicon sequencing approach to short-read amplicons for microbiome analysis https://www.biorxiv.org/content/10.1101/2025.09.04.674341v1
to read!
🦠💊
Ibuprofen and acetaminophen significantly increased mutation frequency and conferred high-level ciprofloxacin resistance
😮🧐
#AMR #Medsky #Episky #IDsky #Microsky
Ibuprofen and acetaminophen significantly increased mutation frequency and conferred high-level ciprofloxacin resistance
😮🧐
#AMR #Medsky #Episky #IDsky #Microsky
💊 Non-antibiotic drugs & resistance
A new study shows common medicines like ibuprofen and acetaminophen can boost E. coli resistance to antibiotics, raising concerns for aged care settings.
🔗 www.nature.com/articles/s44...
#SciComm 🧪 #AMR
A new study shows common medicines like ibuprofen and acetaminophen can boost E. coli resistance to antibiotics, raising concerns for aged care settings.
🔗 www.nature.com/articles/s44...
#SciComm 🧪 #AMR
August 27, 2025 at 10:47 AM
to read!
Wish I met the criteria for this (plenty of 2nd and 3rd authorships under my belt at this point but first authorship is not quite here yet)! Perhaps it will help someone else!
#womeninstem
#womeninstem
Calls for editorial fellows for ESCMID's two journals, @cmijournal.bsky.social & @cmicomms.bsky.social, are closing soon! Don't miss the chance to be part of shaping and growing the journals and your career!
CMI (due 01 Sept): ow.ly/rifq50WBUfy
CMI Comms (due 15 Sept) ow.ly/F43F50WBUfw
#IDSky
CMI (due 01 Sept): ow.ly/rifq50WBUfy
CMI Comms (due 15 Sept) ow.ly/F43F50WBUfw
#IDSky
August 27, 2025 at 10:44 AM
Wish I met the criteria for this (plenty of 2nd and 3rd authorships under my belt at this point but first authorship is not quite here yet)! Perhaps it will help someone else!
#womeninstem
#womeninstem
I've been thinking about how I can use what I am learning and wondering how I can still make that difference in the world that motivated me to apply for a PhD in the first place...this was a good reminder that impact starts with listening.
open.substack.com/pub/healthin...
open.substack.com/pub/healthin...
How to have better vaccine conversations
A guide to connecting across values and shared humanity
open.substack.com
August 27, 2025 at 10:37 AM
I've been thinking about how I can use what I am learning and wondering how I can still make that difference in the world that motivated me to apply for a PhD in the first place...this was a good reminder that impact starts with listening.
open.substack.com/pub/healthin...
open.substack.com/pub/healthin...
Reposted
Utilizing large and diverse bacterial genome datasets to improve the detection and identification of Streptococcus pneumoniae via PCR-based diagnostics. Published #OpenAccess and fee-free in #MGen using a #PublishAndRead agreement:
doi.org/10.1099/mgen...
doi.org/10.1099/mgen...
July 4, 2025 at 9:01 AM
Utilizing large and diverse bacterial genome datasets to improve the detection and identification of Streptococcus pneumoniae via PCR-based diagnostics. Published #OpenAccess and fee-free in #MGen using a #PublishAndRead agreement:
doi.org/10.1099/mgen...
doi.org/10.1099/mgen...
Reposted
Yanomami skin microbiome complexity challenges prevailing concepts of healthy skin. rdcu.be/euNaT
Yanomami skin microbiome complexity challenges prevailing concepts of healthy skin
Nature Communications - Here, the authors show that the skin microbiome of a remote hunter-gatherer Yanomami community harbors environmental microbes that help maintain skin barrier integrity and...
rdcu.be
July 4, 2025 at 9:43 AM
Yanomami skin microbiome complexity challenges prevailing concepts of healthy skin. rdcu.be/euNaT
I'm so excited that we have gotten this one out into the world! Dr. Marshall and Dr. Le are amazing people and stellar scientist. Please give their stories a read!! 😊
#microbiology
#microbiology
Check out the second issue of the GCC AMR Scholars newsletter 🥳 @microann.bsky.social and I got to interview two great scientists! We hope you enjoy!
www.gulfcoastconsortia.org/wp-content/u...
www.gulfcoastconsortia.org/wp-content/u...
www.gulfcoastconsortia.org
July 2, 2025 at 11:31 PM
I'm so excited that we have gotten this one out into the world! Dr. Marshall and Dr. Le are amazing people and stellar scientist. Please give their stories a read!! 😊
#microbiology
#microbiology
Reposted
🎙️ Want to learn more about the role of PBPs (penicillin binding proteins) in cell wall synthesis and how their inhibition impacts bacterial survival?
Check out this episode of Let’s Talk Micro!
📍Link in comments.
#microbiology
Check out this episode of Let’s Talk Micro!
📍Link in comments.
#microbiology
June 28, 2025 at 11:05 PM
🎙️ Want to learn more about the role of PBPs (penicillin binding proteins) in cell wall synthesis and how their inhibition impacts bacterial survival?
Check out this episode of Let’s Talk Micro!
📍Link in comments.
#microbiology
Check out this episode of Let’s Talk Micro!
📍Link in comments.
#microbiology
It's been an intense several months! I'm looking at a to-do list that seems endless, but I'm v excited :) The opportunities awaiting me in the short term are thrilling. Paper 1 is rounding the bend toward being submitted, so now I really get to focus on Paper 2, and after that my PhD defense!!
June 28, 2025 at 11:47 PM
It's been an intense several months! I'm looking at a to-do list that seems endless, but I'm v excited :) The opportunities awaiting me in the short term are thrilling. Paper 1 is rounding the bend toward being submitted, so now I really get to focus on Paper 2, and after that my PhD defense!!
Reposted
Toxic Crusaders introduced nearly 600 California high schoolers to environmental health and public health labs in 2024–2025. Learn how APHL ambassador Ori Roe is helping students in our latest blog: buff.ly/M5WoBZY #workforcedevelopment #environmentalhealth #publichealth
June 25, 2025 at 9:15 PM
Toxic Crusaders introduced nearly 600 California high schoolers to environmental health and public health labs in 2024–2025. Learn how APHL ambassador Ori Roe is helping students in our latest blog: buff.ly/M5WoBZY #workforcedevelopment #environmentalhealth #publichealth
Reposted
@microann.bsky.social and I published a newsletter with the GCC AMR Scholars Committee in the Texas Medical Center! We’re so excited to celebrate our fellow trainees and their cool research! Next issue comes out in 3-months 🥳 www.gulfcoastconsortia.org/wp-content/u...
www.gulfcoastconsortia.org
March 18, 2025 at 11:52 PM
@microann.bsky.social and I published a newsletter with the GCC AMR Scholars Committee in the Texas Medical Center! We’re so excited to celebrate our fellow trainees and their cool research! Next issue comes out in 3-months 🥳 www.gulfcoastconsortia.org/wp-content/u...
Reposted
Phage taxonomy doesn’t have to be taxing, my latest cartoon for the Phage journal. To illustrate 𝘁𝗮𝘅𝗠𝘆𝗣𝗵𝗮𝗴𝗲 lnkd.in/dAXK4sSt @milja001.bsky.social
February 20, 2025 at 9:42 AM
Phage taxonomy doesn’t have to be taxing, my latest cartoon for the Phage journal. To illustrate 𝘁𝗮𝘅𝗠𝘆𝗣𝗵𝗮𝗴𝗲 lnkd.in/dAXK4sSt @milja001.bsky.social
Reposted
christoph: 2 cases where I don't use TSS comp. cells:
· BL21 for protein expression transforms poorly (B strain, not K-12)
· I use(d) electroporation of K-12 target strain carrying pKD46 (ts) for linear transformation of PCR fragments acc. to Datsenko&Wanner
(pmc.ncbi.nlm.nih.gov/articles/PMC...)
· BL21 for protein expression transforms poorly (B strain, not K-12)
· I use(d) electroporation of K-12 target strain carrying pKD46 (ts) for linear transformation of PCR fragments acc. to Datsenko&Wanner
(pmc.ncbi.nlm.nih.gov/articles/PMC...)
January 15, 2025 at 11:32 PM
christoph: 2 cases where I don't use TSS comp. cells:
· BL21 for protein expression transforms poorly (B strain, not K-12)
· I use(d) electroporation of K-12 target strain carrying pKD46 (ts) for linear transformation of PCR fragments acc. to Datsenko&Wanner
(pmc.ncbi.nlm.nih.gov/articles/PMC...)
· BL21 for protein expression transforms poorly (B strain, not K-12)
· I use(d) electroporation of K-12 target strain carrying pKD46 (ts) for linear transformation of PCR fragments acc. to Datsenko&Wanner
(pmc.ncbi.nlm.nih.gov/articles/PMC...)
Reposted
It's such a simple protocol that I invite anyone to please try and report back conditions as well as CFU/mL. Could be fun to crowdsource optimizations. A lil community project that benefits anyone working in microbes :)
Want decent competent cells with at least 10^6 CFU/mL but don't feel like stressing? Try this:
1. Grow ecoli overnight 37C in LB shaking 250rpm
2. Spin down 1mL 15sec 10,000xg
3. Decant and resuspend in 500uL of ice cold TSS buffer. Vortex to resuspend in 3 pulses 1 sec.
Done
1. Grow ecoli overnight 37C in LB shaking 250rpm
2. Spin down 1mL 15sec 10,000xg
3. Decant and resuspend in 500uL of ice cold TSS buffer. Vortex to resuspend in 3 pulses 1 sec.
Done
January 15, 2025 at 10:34 PM
It's such a simple protocol that I invite anyone to please try and report back conditions as well as CFU/mL. Could be fun to crowdsource optimizations. A lil community project that benefits anyone working in microbes :)
Reposted
Autoregulation of the Master Regulator Spo0A Controls Cell-Fate Decisions in Bacillus subtilis
@molecularmicro.bsky.social from Masaya Fujita
onlinelibrary.wiley.com/doi/10.1111/...
@molecularmicro.bsky.social from Masaya Fujita
onlinelibrary.wiley.com/doi/10.1111/...
Autoregulation of the Master Regulator Spo0A Controls Cell‐Fate Decisions in Bacillus subtilis
A proposed model for the autoregulation of Spo0A expression in response to nutrient starvation. The levels of the master regulator Spo0A increase gradually during starvation, resulting in temporal bi...
onlinelibrary.wiley.com
January 16, 2025 at 1:23 PM
Autoregulation of the Master Regulator Spo0A Controls Cell-Fate Decisions in Bacillus subtilis
@molecularmicro.bsky.social from Masaya Fujita
onlinelibrary.wiley.com/doi/10.1111/...
@molecularmicro.bsky.social from Masaya Fujita
onlinelibrary.wiley.com/doi/10.1111/...
One of the wonderful things about going to conferences is hearing all the new advances in the ID field. This particular conference though, the organizers invited a patient to give an address, and I'm pretty sure everyone was in tears. So this year's AMR is going to hold a special place in my heart.
January 17, 2025 at 11:52 AM
One of the wonderful things about going to conferences is hearing all the new advances in the ID field. This particular conference though, the organizers invited a patient to give an address, and I'm pretty sure everyone was in tears. So this year's AMR is going to hold a special place in my heart.
Reposted
My latest cartoon for @newscientist.bsky.social
December 1, 2024 at 11:05 AM
My latest cartoon for @newscientist.bsky.social
Reposted
It's behind a paywall, but if you have access, then you can see how the results of this v brief questionnaire will shape the narrative on the social media platform to use for 🧪, #AcademicSky & #MedSky
Relevant to this audience! @nature.com asks: “Has Bluesky replaced X for scientists? Take Nature’s poll”: www.nature.com/articles/d41... 🧪
Has Bluesky replaced X for scientists? Take Nature’s poll
The research community has flocked to the social-media platform Bluesky. Tell us about your experience.
www.nature.com
January 14, 2025 at 11:51 PM
It's behind a paywall, but if you have access, then you can see how the results of this v brief questionnaire will shape the narrative on the social media platform to use for 🧪, #AcademicSky & #MedSky
Reposted
Interesting to #IDSky & #ClinMicro
FINALLY! Our discovery of a very specific and sensitive serological target for schistosomiasis diagnostics is UP. Please, enjoy our shout-out for glycans and the importance of anticipating diagnostics needs 🧪🩸🪱 journals.asm.org/doi/10.1128/...
Identification of a circulating carbohydrate antigen as a highly specific and sensitive target for schistosomiasis serology | Journal of Clinical Microbiology
The WHO 2030 roadmap deems diagnostics developments for schistosomiasis critically
needed. Here we present identification of an antibody target with superior performance
compared to traditionally used...
journals.asm.org
January 15, 2025 at 8:33 AM
Interesting to #IDSky & #ClinMicro
I'm trying to do better about talking to people outside my lab, which is great. But this means that I've found myself in a situation where I have to talk about my dissertation project in front of people who could be my boss someday and I'm feeling the stresss. Love going to conferences so much lol.
January 15, 2025 at 11:24 AM
I'm trying to do better about talking to people outside my lab, which is great. But this means that I've found myself in a situation where I have to talk about my dissertation project in front of people who could be my boss someday and I'm feeling the stresss. Love going to conferences so much lol.
It's day 1 of wrangling my way through writing a review and revising my original research manuscript. I'm using Wendy Laura Belcher's wkbk even though I don't think it's really written for STEM, but it's the best guide I've come across. #womeninstem if you know of any other guides please recommend!
January 4, 2025 at 1:30 PM
It's day 1 of wrangling my way through writing a review and revising my original research manuscript. I'm using Wendy Laura Belcher's wkbk even though I don't think it's really written for STEM, but it's the best guide I've come across. #womeninstem if you know of any other guides please recommend!
First plates of the new year! Now if the bacteria in question will kindly agree to be cultivated, then 2025 will be truly off to a good start.
January 2, 2025 at 5:00 PM
First plates of the new year! Now if the bacteria in question will kindly agree to be cultivated, then 2025 will be truly off to a good start.