Matthew McCallum
@mattmccallum.bsky.social
Acting Instructor at UW
Structural biology - Protein design - Microbiology - Virology - Evolution
(he/him)
Structural biology - Protein design - Microbiology - Virology - Evolution
(he/him)
Reposted by Matthew McCallum
Delighted to share the peer-reviewed version of our article describing a prefusion-stabilized MARV GP vaccine immunogen and a best-in-class MARV neutralizing and protective antibody!
Led by @aminaddetia.bsky.social with @virbiotechnology.bsky.social
@hhmi.org
www.nature.com/articles/s41...
Led by @aminaddetia.bsky.social with @virbiotechnology.bsky.social
@hhmi.org
www.nature.com/articles/s41...
Potent neutralization of Marburg virus by a vaccine-elicited antibody - Nature
Nature - Potent neutralization of Marburg virus by a vaccine-elicited antibody
www.nature.com
November 12, 2025 at 4:20 PM
Delighted to share the peer-reviewed version of our article describing a prefusion-stabilized MARV GP vaccine immunogen and a best-in-class MARV neutralizing and protective antibody!
Led by @aminaddetia.bsky.social with @virbiotechnology.bsky.social
@hhmi.org
www.nature.com/articles/s41...
Led by @aminaddetia.bsky.social with @virbiotechnology.bsky.social
@hhmi.org
www.nature.com/articles/s41...
Reposted by Matthew McCallum
Our latest work on #COVID immunity and imprinting in humans.
Led by Ale Tortorici and Kaiti Sprouse with @aminaddetia.bsky.social and several others!
www.biorxiv.org/content/10.1...
Led by Ale Tortorici and Kaiti Sprouse with @aminaddetia.bsky.social and several others!
www.biorxiv.org/content/10.1...
Repeated COVID-19 vaccine boosters elicit variant-specific memory B cells in humans
The first exposure to a pathogen or an antigen profoundly impacts immune responses upon subsequent encounter with related pathogens. This immune imprinting explains that infection or vaccination with ...
www.biorxiv.org
October 21, 2025 at 3:42 AM
Our latest work on #COVID immunity and imprinting in humans.
Led by Ale Tortorici and Kaiti Sprouse with @aminaddetia.bsky.social and several others!
www.biorxiv.org/content/10.1...
Led by Ale Tortorici and Kaiti Sprouse with @aminaddetia.bsky.social and several others!
www.biorxiv.org/content/10.1...
Reposted by Matthew McCallum
New paper! Specific PilA and PilY1 sequences make PilU dispensable for T4P-dependent twitching motility in P. aeruginosa. However, most suppressors still had high cAMP levels from loss of PilU, showing that motility and surface sensing responses can be uncoupled.🧐 ⏩ journals.asm.org/doi/10.1128/...
Separation of Pseudomonas aeruginosa type IV pilus-dependent twitching motility and surface-sensing responses | mBio
The ability of bacteria to sense and respond to contact with surfaces is important
for triggering changes in secondary messenger levels and gene expression, leading
to the formation of biofilms and in...
journals.asm.org
October 7, 2025 at 5:09 PM
New paper! Specific PilA and PilY1 sequences make PilU dispensable for T4P-dependent twitching motility in P. aeruginosa. However, most suppressors still had high cAMP levels from loss of PilU, showing that motility and surface sensing responses can be uncoupled.🧐 ⏩ journals.asm.org/doi/10.1128/...
Reposted by Matthew McCallum
Excited to be part of this collaboration with the Burrows lab exploring twitching and surface surface sensing by P. aeruginosa. This work further delineates a role for the T4P motor proteins and pili for surface sensing.
journals.asm.org/doi/10.1128/...
@asm.org
journals.asm.org/doi/10.1128/...
@asm.org
October 7, 2025 at 8:32 PM
Excited to be part of this collaboration with the Burrows lab exploring twitching and surface surface sensing by P. aeruginosa. This work further delineates a role for the T4P motor proteins and pili for surface sensing.
journals.asm.org/doi/10.1128/...
@asm.org
journals.asm.org/doi/10.1128/...
@asm.org
Reposted by Matthew McCallum
Outbreaks of Marburg virus (MARV) disease have been increasingly more frequent recently. Here we describe a prefusion-stabilized MARV GP and a best-in-class MARV neutralizing antibody!
Led by @aminaddetia.bsky.social with @virbiotechnology.bsky.social
@hhmi.org
www.biorxiv.org/content/10.1...
Led by @aminaddetia.bsky.social with @virbiotechnology.bsky.social
@hhmi.org
www.biorxiv.org/content/10.1...
Potent neutralization of Marburg virus by a vaccine-elicited monoclonal antibody
Marburg virus (MARV) is a filovirus that causes a severe and often lethal hemorrhagic fever. Despite the increasing frequency of MARV outbreaks, no vaccines or therapeutics are licensed for use in hum...
www.biorxiv.org
May 18, 2025 at 8:40 PM
Outbreaks of Marburg virus (MARV) disease have been increasingly more frequent recently. Here we describe a prefusion-stabilized MARV GP and a best-in-class MARV neutralizing antibody!
Led by @aminaddetia.bsky.social with @virbiotechnology.bsky.social
@hhmi.org
www.biorxiv.org/content/10.1...
Led by @aminaddetia.bsky.social with @virbiotechnology.bsky.social
@hhmi.org
www.biorxiv.org/content/10.1...
Reposted by Matthew McCallum
Delighted to share our most recent work on designing broadly protective sarbecovirus vaccines and defining the path forward for other coronaviruses.
Led by @aminaddetia.bsky.social
@hhmi.org
www.biorxiv.org/content/10.1...
Led by @aminaddetia.bsky.social
@hhmi.org
www.biorxiv.org/content/10.1...
Phylogeny-driven design of broadly protective sarbecovirus receptor-binding domain nanoparticle vaccines
Vaccines against emerging SARS-CoV-2 variants and sarbecoviruses with pandemic potential must elicit a robust humoral immune response in a population imprinted with the SARS-CoV-2 spike (S) protein. H...
www.biorxiv.org
May 14, 2025 at 2:21 PM
Delighted to share our most recent work on designing broadly protective sarbecovirus vaccines and defining the path forward for other coronaviruses.
Led by @aminaddetia.bsky.social
@hhmi.org
www.biorxiv.org/content/10.1...
Led by @aminaddetia.bsky.social
@hhmi.org
www.biorxiv.org/content/10.1...
Reposted by Matthew McCallum
Thrilled to announce my first PhD publication in Nature Comms! We determined structures of the Tad pilus ATPase CpaF from Caulobacter. We use these structures to propose how CpaF employs a rotary mechanism of catalysis to drive Tad pilus assembly.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Conformational changes in the motor ATPase CpaF facilitate a rotary mechanism of Tad pilus assembly - Nature Communications
The bacterial Tad pilus extends and retracts using a single bifunctional ATPase CpaF. Here, the authors employ cryo-EM, fluorescent microscopy, and AlphaFold modelling to propose how a rotary mechanis...
www.nature.com
April 24, 2025 at 3:29 AM
Thrilled to announce my first PhD publication in Nature Comms! We determined structures of the Tad pilus ATPase CpaF from Caulobacter. We use these structures to propose how CpaF employs a rotary mechanism of catalysis to drive Tad pilus assembly.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Matthew McCallum
The vaccine against Shingles helps protect against dementia, results of a natural experiment, adding to prior evidence
"implications are profound"
New @nature.com
www.nature.com/articles/d41...
nature.com/articles/s41...
"implications are profound"
New @nature.com
www.nature.com/articles/d41...
nature.com/articles/s41...
A natural experiment on the effect of herpes zoster vaccination on dementia - Nature
Using a natural experiment that avoids common bias concerns, this study finds that the live-attenuated shingles vaccine reduced the probability of a new dementia diagnosis within a follow-up period of...
nature.com
April 2, 2025 at 3:29 PM
The vaccine against Shingles helps protect against dementia, results of a natural experiment, adding to prior evidence
"implications are profound"
New @nature.com
www.nature.com/articles/d41...
nature.com/articles/s41...
"implications are profound"
New @nature.com
www.nature.com/articles/d41...
nature.com/articles/s41...
Reposted by Matthew McCallum
Deconvolution to restore cryo-EM maps with anisotropic resolution https://www.biorxiv.org/content/10.1101/2025.02.23.639707v1
March 1, 2025 at 6:47 PM
Deconvolution to restore cryo-EM maps with anisotropic resolution https://www.biorxiv.org/content/10.1101/2025.02.23.639707v1
Reposted by Matthew McCallum
Structure-based design of an immunogenic, conformationally stabilized FimH antigen for a urinary tract infection vaccine pubmed.ncbi.nlm.nih.gov/39970181/ #cryoem
February 21, 2025 at 2:34 AM
Structure-based design of an immunogenic, conformationally stabilized FimH antigen for a urinary tract infection vaccine pubmed.ncbi.nlm.nih.gov/39970181/ #cryoem
Reposted by Matthew McCallum
With participation of Jimin Lee, Jack Brown, Risako Gen, @dabiophysicist.bsky.social, Cameron Stewart in our lab & in collaboration with the labs of Huan Yan and Tyler Starr
www.cell.com/cell/fulltex...
www.cell.com/cell/fulltex...
Multiple independent acquisitions of ACE2 usage in MERS-related coronaviruses
Two coronaviruses related to MERS-CoV, which infect European Pipistrellus bats, have
been found to use ACE2 as their receptor. These viruses target an ACE2 surface region
distant from those recognized...
www.cell.com
February 7, 2025 at 4:12 PM
With participation of Jimin Lee, Jack Brown, Risako Gen, @dabiophysicist.bsky.social, Cameron Stewart in our lab & in collaboration with the labs of Huan Yan and Tyler Starr
www.cell.com/cell/fulltex...
www.cell.com/cell/fulltex...
Reposted by Matthew McCallum
Our long-awaited study in collaboration with the Schmidt lab on H5Nx influenza recognition by naive B cells is out! #antibody #bcell #flu #avianflu #cryoEM
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Human naïve B cells recognize prepandemic influenza virus hemagglutinins
Antibodies targeting the influenza hemagglutinin from potentially pandemic H5Nx viruses can be isolated from naïve individuals.
www.science.org
January 24, 2025 at 10:06 PM
Reposted by Matthew McCallum
Here, we show that SARS-CoV-2 nsp1 inhibits translation in a wide range of mammalian cells from known hosts (deer, mink, cat, dog,...) & unveils the architecture of a Rhinolophus bat ribosome with mRNA channel obstructed by nsp1!
Led by Risako Gen
@hhmi.bsky.social
www.biorxiv.org/content/10.1...
Led by Risako Gen
@hhmi.bsky.social
www.biorxiv.org/content/10.1...
SARS-CoV-2 nsp1 mediates broad inhibition of translation in mammals
SARS-CoV-2 nonstructural protein 1 (nsp1) promotes innate immune evasion by inhibiting host translation in human cells. However, the role of nsp1 in other host species remains elusive, especially in b...
www.biorxiv.org
January 16, 2025 at 10:21 PM
Here, we show that SARS-CoV-2 nsp1 inhibits translation in a wide range of mammalian cells from known hosts (deer, mink, cat, dog,...) & unveils the architecture of a Rhinolophus bat ribosome with mRNA channel obstructed by nsp1!
Led by Risako Gen
@hhmi.bsky.social
www.biorxiv.org/content/10.1...
Led by Risako Gen
@hhmi.bsky.social
www.biorxiv.org/content/10.1...
Indeed - thank you David Goodsell for bringing science to life with art! Seeing the pilus motor featured is still my favourite grad school memory
doi.org/10.2210/rcsb...
doi.org/10.2210/rcsb...
January 7, 2025 at 5:02 PM
Indeed - thank you David Goodsell for bringing science to life with art! Seeing the pilus motor featured is still my favourite grad school memory
doi.org/10.2210/rcsb...
doi.org/10.2210/rcsb...
Reposted by Matthew McCallum
Structure of the Pseudomonas aeruginosa PAO1 Type IV pilus
Finally out in PLOS Pathogens. Congrats Hannah Ochner and co-authors. @mrclmb.bsky.social
journals.plos.org/plospathogen...
Finally out in PLOS Pathogens. Congrats Hannah Ochner and co-authors. @mrclmb.bsky.social
journals.plos.org/plospathogen...
December 16, 2024 at 3:53 PM
Structure of the Pseudomonas aeruginosa PAO1 Type IV pilus
Finally out in PLOS Pathogens. Congrats Hannah Ochner and co-authors. @mrclmb.bsky.social
journals.plos.org/plospathogen...
Finally out in PLOS Pathogens. Congrats Hannah Ochner and co-authors. @mrclmb.bsky.social
journals.plos.org/plospathogen...
Our latest work stabilizing the gB fusion protein.
This paves the way for next-gen vaccines against Herpesviruses implicated in cancer, MS, and dementia.
🔗 www.biorxiv.org/content/10.1...
#herpesvirus #EBV #HSV #CMV #proteindesign #virology #immunology
This paves the way for next-gen vaccines against Herpesviruses implicated in cancer, MS, and dementia.
🔗 www.biorxiv.org/content/10.1...
#herpesvirus #EBV #HSV #CMV #proteindesign #virology #immunology
November 17, 2024 at 8:12 PM
Our latest work stabilizing the gB fusion protein.
This paves the way for next-gen vaccines against Herpesviruses implicated in cancer, MS, and dementia.
🔗 www.biorxiv.org/content/10.1...
#herpesvirus #EBV #HSV #CMV #proteindesign #virology #immunology
This paves the way for next-gen vaccines against Herpesviruses implicated in cancer, MS, and dementia.
🔗 www.biorxiv.org/content/10.1...
#herpesvirus #EBV #HSV #CMV #proteindesign #virology #immunology