@karl-petri.bsky.social
Emmy Noether group leader @Uniklinikum_Wue & @Uni_WUE. CRISPR, CAR-T, Hemato-Oncology, previously @JoungLab, views my own
Pinned
Next-generation T cell immunotherapies engineered with CRISPR base and prime editing: challenges and opportunities
Nature Reviews Clinical Oncology - Several persistent challenges limit the efficacy and applicability of adoptive T cell therapies for cancer, including suboptimal function and/or persistence in...
rdcu.be
🚀 New Review Alert!
In @natrevclinonc.nature.com, we explore how CRISPR base & prime editing can advance T cell immunotherapies — from augmenting function to optimizing production.
Read it here 👉 rdcu.be/eHcms
In @natrevclinonc.nature.com, we explore how CRISPR base & prime editing can advance T cell immunotherapies — from augmenting function to optimizing production.
Read it here 👉 rdcu.be/eHcms
Reposted
You like engineering T cells, but you are missing the right flavor?
Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1
We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt
𝐏rogrammable
𝐀nd
𝐒ite-specific
𝐓ransgene
𝐀ddition
🧪
Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1
We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt
𝐏rogrammable
𝐀nd
𝐒ite-specific
𝐓ransgene
𝐀ddition
🧪
Versatile and efficient non-viral integration of large transgenes in human T cells via CRISPR knock-in and engineered integrases
Current gene transfer methods often lack the precision, versatility, or efficiency when integrating large transgenes, limiting the ability to engineer therapeutic T-cells with more complex payloads. H...
www.biorxiv.org
September 19, 2025 at 5:30 AM
You like engineering T cells, but you are missing the right flavor?
Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1
We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt
𝐏rogrammable
𝐀nd
𝐒ite-specific
𝐓ransgene
𝐀ddition
🧪
Check out 'one-pot' PASTA recipe 🔥🍝: www.biorxiv.org/content/10.1101/2025.09.10.675267v1
We combined a unique mix of CRISPR knock-in with serine integrases for easy-to-adopt
𝐏rogrammable
𝐀nd
𝐒ite-specific
𝐓ransgene
𝐀ddition
🧪
🚀 New Review Alert!
In @natrevclinonc.nature.com, we explore how CRISPR base & prime editing can advance T cell immunotherapies — from augmenting function to optimizing production.
Read it here 👉 rdcu.be/eHcms
In @natrevclinonc.nature.com, we explore how CRISPR base & prime editing can advance T cell immunotherapies — from augmenting function to optimizing production.
Read it here 👉 rdcu.be/eHcms
Next-generation T cell immunotherapies engineered with CRISPR base and prime editing: challenges and opportunities
Nature Reviews Clinical Oncology - Several persistent challenges limit the efficacy and applicability of adoptive T cell therapies for cancer, including suboptimal function and/or persistence in...
rdcu.be
September 19, 2025 at 11:20 AM
🚀 New Review Alert!
In @natrevclinonc.nature.com, we explore how CRISPR base & prime editing can advance T cell immunotherapies — from augmenting function to optimizing production.
Read it here 👉 rdcu.be/eHcms
In @natrevclinonc.nature.com, we explore how CRISPR base & prime editing can advance T cell immunotherapies — from augmenting function to optimizing production.
Read it here 👉 rdcu.be/eHcms
Reposted
Most genome editing work to date has been DNA based. A new paper out today assessed a single dose of epigenetic editing (thru PCSK9) for marked (~70%) and durable reduction of LDL cholesterol [in non-human primates]
www.nature.com/articles/s41...
www.nature.com/articles/s41...
A potent epigenetic editor targeting human PCSK9 for durable reduction of low-density lipoprotein cholesterol levels - Nature Medicine
Epigenetic editing of PCSK9 in the liver, designed to induce DNA methylation and silence gene expression, achieved long-lasting reductions in LDL cholesterol levels in both transgenic mice expressing ...
www.nature.com
February 10, 2025 at 4:01 PM
Most genome editing work to date has been DNA based. A new paper out today assessed a single dose of epigenetic editing (thru PCSK9) for marked (~70%) and durable reduction of LDL cholesterol [in non-human primates]
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted
Out today in @naturegenet.bsky.social -- PERFF-seq! With @tsionabay.bsky.social , @ronanchaligne.bsky.social, Bob Stickels, Meril Takizawa, + Ansu Satpathy, we describe this new assay to study rare populations with programmable nucleic acid cytometry. 1/n
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Transcript-specific enrichment enables profiling of rare cell states via single-cell RNA sequencing - Nature Genetics
Programmable Enrichment via RNA FlowFISH by sequencing (PERFF-seq) isolates rare cells based on RNA marker transcripts for single-cell RNA sequencing profiling of complex tissues, with applicability t...
www.nature.com
January 8, 2025 at 1:30 PM
Out today in @naturegenet.bsky.social -- PERFF-seq! With @tsionabay.bsky.social , @ronanchaligne.bsky.social, Bob Stickels, Meril Takizawa, + Ansu Satpathy, we describe this new assay to study rare populations with programmable nucleic acid cytometry. 1/n
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted
Excited to share our study on spatio-temporal reconstruction of cardiac scar formation led by Andy Chan. We combine scRNA-seq with spatial transcriptomics to resolve the fibrotic niche and the niche of dedifferentiating cardiomyocytes.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
November 16, 2024 at 12:26 PM
Excited to share our study on spatio-temporal reconstruction of cardiac scar formation led by Andy Chan. We combine scRNA-seq with spatial transcriptomics to resolve the fibrotic niche and the niche of dedifferentiating cardiomyocytes.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...