puri fortes
@forteslab.bsky.social
Fascinated about non coding RNA and microproteins. Willing for clinical translation. Energy from family and friends. Believer in RNA biology and therapy. Living in CIMA, at University of Navarra.
Reposted by puri fortes
Identified in the 1970s, the precise molecular function of the alternative initiation factor eIF2A has remained unclear. Here, we map its interactome and uncover a surprising role in ribosome-associated quality control (RQC) upon ribosome stalling. 1/x
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
The alternative initiation factor eIF2A regulates 40S subunit turnover in ribosome-associated quality control
The noncanonical translation initiation factor eIF2A plays critical roles in diverse cellular processes, including the integrated stress response, neurodegeneration and tumorigenesis. However, the pre...
www.biorxiv.org
May 26, 2025 at 9:35 PM
Identified in the 1970s, the precise molecular function of the alternative initiation factor eIF2A has remained unclear. Here, we map its interactome and uncover a surprising role in ribosome-associated quality control (RQC) upon ribosome stalling. 1/x
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
I had missed this super nice story!
An ultraconserved snoRNA-like element in long noncoding RNA CRNDE promotes ribosome biogenesis and cell proliferation: Molecular Cell www.cell.com/molecular-ce...
An ultraconserved snoRNA-like element in long noncoding RNA CRNDE promotes ribosome biogenesis and cell proliferation: Molecular Cell www.cell.com/molecular-ce...
An ultraconserved snoRNA-like element in long noncoding RNA CRNDE promotes ribosome biogenesis and cell proliferation
Cancer cells frequently enhance ribosome production to support rapid growth. Here,
Lee et al. demonstrate that an isoform of lncRNA CRNDE containing an ultraconserved
element (CRNDEUCE) promotes 60S r...
www.cell.com
May 19, 2025 at 2:57 PM
I had missed this super nice story!
An ultraconserved snoRNA-like element in long noncoding RNA CRNDE promotes ribosome biogenesis and cell proliferation: Molecular Cell www.cell.com/molecular-ce...
An ultraconserved snoRNA-like element in long noncoding RNA CRNDE promotes ribosome biogenesis and cell proliferation: Molecular Cell www.cell.com/molecular-ce...
Reposted by puri fortes
Ribosomes modulate transcriptome abundance via generalized frameshift and out-of-frame mRNA decay
Ribosomes modulate transcriptome abundance via generalized frameshift and out-of-frame mRNA decay
Zhang et al. identify a regulatory mechanism for how cells adapt to nutrient scarcity through widespread −1 ribosomal frameshifts, culminating in accelerated mRNA decay. This process, dependent on codon optimality and conserved across species, establishes direct feedback coupling the translation of new proteins with the stability of the mRNA that encodes for them.
dlvr.it
May 17, 2025 at 3:19 PM
Ribosomes modulate transcriptome abundance via generalized frameshift and out-of-frame mRNA decay
Reposted by puri fortes
*LESS THAN 1 MONTH TO SUBMIT ABSTRACTS*
Sign up for the #GordonResearchConference on #microproteins and the #darkproteome now!
Scholarships are available for students. Apply by June 1 to be eligible!!!
www.grc.org/decoding-mic...
Sign up for the #GordonResearchConference on #microproteins and the #darkproteome now!
Scholarships are available for students. Apply by June 1 to be eligible!!!
www.grc.org/decoding-mic...
2025 Decoding Microproteins Across Evolution and Disease Conference GRC
The 2025 Gordon Research Conference on Decoding Microproteins Across Evolution and Disease will be held in Castelldefels, Barcelona Spain. Apply today to reserve your spot.
www.grc.org
May 11, 2025 at 5:17 PM
*LESS THAN 1 MONTH TO SUBMIT ABSTRACTS*
Sign up for the #GordonResearchConference on #microproteins and the #darkproteome now!
Scholarships are available for students. Apply by June 1 to be eligible!!!
www.grc.org/decoding-mic...
Sign up for the #GordonResearchConference on #microproteins and the #darkproteome now!
Scholarships are available for students. Apply by June 1 to be eligible!!!
www.grc.org/decoding-mic...
Reposted by puri fortes
The timeline of co-transcriptional #mRNA processing and #modification may have to be revised: cleavage, termination, and #m6A deposition may occur earlier than most #splicing! Potential textbook changing study in bioRxiv by edueyras.bsky.social & Rippei Hayashi labs!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Single-molecule multimodal timing of in vivo mRNA synthesis
mRNA synthesis requires extensive pre-mRNA maturation, the organisation of which remains unclear. Here, we directly sequence pre-mRNA without metabolic labelling or amplification to resolve transcript...
www.biorxiv.org
April 29, 2025 at 8:31 PM
The timeline of co-transcriptional #mRNA processing and #modification may have to be revised: cleavage, termination, and #m6A deposition may occur earlier than most #splicing! Potential textbook changing study in bioRxiv by edueyras.bsky.social & Rippei Hayashi labs!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by puri fortes
I am happy to share the latest paper from the lab. We detail a tripartite cell-free translation system based on the separation of mRNAs, ribosomes and ribosome-depleted cytoplasmic lysate from human cells, allowing for flexible reconstitution of translation reaction.
#RNA #Ribosome
#RNA #Ribosome
New Article! A tripartite cell-free translation system to study mammalian translation
A tripartite cell-free translation system to study mammalian translation
Nature Protocols, Published online: 16 April 2025; doi:10.1038/s41596-025-01155-7This protocol presents a tripartite mammalian cell-free translation system, comprising cytoplasmic and ribosome fractions combined with endogenous or synthetic mRNAs, permitting flexible reconstitution and careful dissection of the mechanisms of translation.
bit.ly
April 16, 2025 at 10:47 AM
Reposted by puri fortes
Will be taking a deep dive into this today. Congrats to Joe Lobel and Nick Ingolia! #RNAsky #RNAbiology
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Deciphering disordered regions controlling mRNA decay in high-throughput - Nature
Using high-throughput functional profiling, how disordered regions control mRNA stability and translation is explored.
www.nature.com
April 24, 2025 at 10:53 AM
Will be taking a deep dive into this today. Congrats to Joe Lobel and Nick Ingolia! #RNAsky #RNAbiology
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by puri fortes
Excited to announce a new paper out today in @cp-cell.bsky.social discovering RNA mis-splicing derived neoantigens & their cognate T cell receptors (TCRs) as well as characterization of endogenous neoantigen T cells in leukemia patients.
authors.elsevier.com/sd/article/S...
authors.elsevier.com/sd/article/S...
ScienceDirect.com | Science, health and medical journals, full text articles and books.
authors.elsevier.com
April 23, 2025 at 8:30 PM
Excited to announce a new paper out today in @cp-cell.bsky.social discovering RNA mis-splicing derived neoantigens & their cognate T cell receptors (TCRs) as well as characterization of endogenous neoantigen T cells in leukemia patients.
authors.elsevier.com/sd/article/S...
authors.elsevier.com/sd/article/S...