Nature Protocols
@natprot.nature.com
New Article! High-throughput identification of endogenous biomolecular condensates and phase-separating proteins
High-throughput identification of endogenous biomolecular condensates and phase-separating proteins
Nature Protocols, Published online: 12 February 2026; doi:10.1038/s41596-025-01327-5This protocol identifies endogenous biomolecular condensates by combining sucrose density gradient centrifugation with quantitative mass spectrometry, enabling proteome-wide, stimulus-responsive profiling of phase-separating proteins.
dlvr.it
February 12, 2026 at 2:44 PM
New Article! High-throughput identification of endogenous biomolecular condensates and phase-separating proteins
New Article! Multichannel genomic recording of biological information with ENGRAM
Multichannel genomic recording of biological information with ENGRAM
Nature Protocols, Published online: 11 February 2026; doi:10.1038/s41596-025-01322-wThis Protocol details the use of enhancer-mediated genomic recording of activity in multiplex (ENGRAM) for molecular recording. This system converts the transient activity of cis-regulatory elements into stable genomic records that can be retrospectively recovered via DNA sequencing.
dlvr.it
February 12, 2026 at 12:18 AM
New Article! Multichannel genomic recording of biological information with ENGRAM
New Article! Simplified ischemia-free liver transplantation with continuous normothermic machine perfusion
Simplified ischemia-free liver transplantation with continuous normothermic machine perfusion
Nature Protocols, Published online: 06 February 2026; doi:10.1038/s41596-025-01321-xThis Protocol describes a simplified ischemia-free liver transplant procedure, where the portal vein is directly cannulated into the donor portal vein, eliminating the need for an interposition vein.
dlvr.it
February 6, 2026 at 12:12 PM
New Article! Simplified ischemia-free liver transplantation with continuous normothermic machine perfusion
New Article! Designing and testing CRISPRi-based synthetic gene circuits in plants
Designing and testing CRISPRi-based synthetic gene circuits in plants
Nature Protocols, Published online: 04 February 2026; doi:10.1038/s41596-025-01312-yThis protocol details the design principles and procedures for building programmable CRISPRi-based gene circuits, and for testing individual components and the assembled circuits in various plant species using a high-throughput protoplast-based assay.
dlvr.it
February 4, 2026 at 3:43 PM
New Article! Designing and testing CRISPRi-based synthetic gene circuits in plants
New Article! Conjugation of hydrophobic drugs to motile pRNA 4WJ nanoparticles for spontaneous tumor targeting and undetectable toxicity
Conjugation of hydrophobic drugs to motile pRNA 4WJ nanoparticles for spontaneous tumor targeting and undetectable toxicity
Nature Protocols, Published online: 03 February 2026; doi:10.1038/s41596-025-01306-wThis protocol describes the synthesis, purification and assembly of stable branched 4-way junction (4WJ) RNA nanoparticles conjugated with multiple hydrophobic chemotherapeutic drug molecules to target specific tumors with minimal side effects.
dlvr.it
February 4, 2026 at 1:05 AM
New Article! Conjugation of hydrophobic drugs to motile pRNA 4WJ nanoparticles for spontaneous tumor targeting and undetectable toxicity
New Article! Secreted luciferases as a minimally invasive 3R-compliant tool for accurate monitoring of tumor burden
Secreted luciferases as a minimally invasive 3R-compliant tool for accurate monitoring of tumor burden
Nature Protocols, Published online: 03 February 2026; doi:10.1038/s41596-025-01315-9This protocol details in vitro and in vivo approaches for labeling tumor cells with secreted luciferases to enable precise, longitudinal monitoring of tumor growth in mice, through the analysis of small blood samples.
dlvr.it
February 3, 2026 at 3:11 PM
New Article! Secreted luciferases as a minimally invasive 3R-compliant tool for accurate monitoring of tumor burden
New Article! INFOGEST Quant: standardized in vitro determination of digestibility and DIAAS of dietary proteins based on the INFOGEST static digestion model
INFOGEST Quant: standardized in vitro determination of digestibility and DIAAS of dietary proteins based on the INFOGEST static digestion model
Nature Protocols, Published online: 03 February 2026; doi:10.1038/s41596-025-01307-9This Protocol Extension is an adapted version of the authors’ previous INFOGEST static digestion model (INFOGEST 2.0), with an added workflow for the quantification of total protein digestibility, individual amino acid digestibility and digestible indispensable amino acid score.
dlvr.it
February 3, 2026 at 2:41 PM
New Article! INFOGEST Quant: standardized in vitro determination of digestibility and DIAAS of dietary proteins based on the INFOGEST static digestion model
New Article! Magnetoencephalography in human neuroscience research: planning, piloting, implementation and quality assurance
Magnetoencephalography in human neuroscience research: planning, piloting, implementation and quality assurance
Nature Protocols, Published online: 02 February 2026; doi:10.1038/s41596-025-01295-wA protocol for the planning and implementation of magnetoencephalography experiments across nonclinical and clinical settings, including the methodological considerations required for designing multidisciplinary, population-based studies.
dlvr.it
February 2, 2026 at 3:43 PM
New Article! Magnetoencephalography in human neuroscience research: planning, piloting, implementation and quality assurance
New Article! Direct delivery of assay reagents to extracellular vesicles in liquid biopsies for biomarker analysis
Direct delivery of assay reagents to extracellular vesicles in liquid biopsies for biomarker analysis
Nature Protocols, Published online: 30 January 2026; doi:10.1038/s41596-025-01317-7This protocol outlines an extracellular vesicle detection approach in which reagent-loaded liposomes fuse with extracellular vesicles directly in patient blood to sensitively detect RNA within the extracellular vesicles.
dlvr.it
January 30, 2026 at 2:28 PM
New Article! Direct delivery of assay reagents to extracellular vesicles in liquid biopsies for biomarker analysis
New Article! Integrated fluorescence light microscopy-guided cryo-focused ion beam-milling for in situ montage cryo-ET
Integrated fluorescence light microscopy-guided cryo-focused ion beam-milling for in situ montage cryo-ET
Nature Protocols, Published online: 30 January 2026; doi:10.1038/s41596-025-01284-zA protocol for cryogenic 3D correlative focused ion beam milling using an integrated fluorescence light microscope and montage cryo-ET for nonadherent and adherent mammalian cells, as well as primary Drosophila melanogaster neurons.
dlvr.it
January 30, 2026 at 11:25 AM
New Article! Integrated fluorescence light microscopy-guided cryo-focused ion beam-milling for in situ montage cryo-ET
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This webinar was part of Love Methods Week 2026.
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Methods Mythbusting: Insights from Journal Editors and protocols.io
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January 29, 2026 at 1:41 PM
Watch the webinar: "Methods Mythbusting: Insights from Journal Editors and protocols.io"
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This webinar was part of Love Methods Week 2026.
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Reposted by Nature Protocols
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Tutorial: annotation of animal genomes
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Tutorial: annotation of animal genomes
www.nature.com/articles/s41...
January 28, 2026 at 9:07 PM
Review @natprot.nature.com @garybader.bsky.social
Tutorial: annotation of animal genomes
www.nature.com/articles/s41...
Tutorial: annotation of animal genomes
www.nature.com/articles/s41...
New Article! CellRank: consistent and data view agnostic fate mapping for single-cell genomics
CellRank: consistent and data view agnostic fate mapping for single-cell genomics
Nature Protocols, Published online: 29 January 2026; doi:10.1038/s41596-025-01314-wCellRank enables cell fate mapping from single-cell RNA sequencing data in a consistent and scalable manner, relying on different views of the data such as pseudotime or stemness potential, RNA velocity and experimental time points.
dlvr.it
January 29, 2026 at 1:14 PM
New Article! CellRank: consistent and data view agnostic fate mapping for single-cell genomics
New Article! Tutorial: annotation of animal genomes
Tutorial: annotation of animal genomes
Nature Protocols, Published online: 28 January 2026; doi:10.1038/s41596-025-01301-1This Tutorial integrates state-of-the-art tools into a streamlined workflow to create high-quality annotations for animal genomes, assigning gene symbols and annotating repeat regions for both protein-coding and non-coding RNA genes, before assessing annotation quality.
dlvr.it
January 28, 2026 at 11:42 AM
New Article! Tutorial: annotation of animal genomes
New Article! Putting mammalian early embryonic cells into dormancy
Putting mammalian early embryonic cells into dormancy
Nature Protocols, Published online: 26 January 2026; doi:10.1038/s41596-025-01303-zThe authors provide a suite of in vitro protocols through which mouse and human pluripotent stem cells, mouse blastocysts and human blastoids can be reversibly induced to enter a diapause-like dormant state via pharmacological inhibition of mTOR
dlvr.it
January 27, 2026 at 12:12 AM
New Article! Putting mammalian early embryonic cells into dormancy
New Article! Investigation of mechanical forces during multicellular early angiogenic sprouting by three-dimensional traction force microscopy in hydrogel matrices
Investigation of mechanical forces during multicellular early angiogenic sprouting by three-dimensional traction force microscopy in hydrogel matrices
Nature Protocols, Published online: 23 January 2026; doi:10.1038/s41596-025-01275-0Investigating mechanical forces during angiogenic sprouting by three-dimensional traction force microscopy (TFM) in hydrogel matrices, including steps to analyze and interpret cell–extracellular matrix forces using open-source TFMLAB software.
dlvr.it
January 23, 2026 at 10:20 AM
New Article! Investigation of mechanical forces during multicellular early angiogenic sprouting by three-dimensional traction force microscopy in hydrogel matrices
New Article! Screening high-quality lignins extracted from biomass to prepare biobased wood adhesives
Screening high-quality lignins extracted from biomass to prepare biobased wood adhesives
Nature Protocols, Published online: 22 January 2026; doi:10.1038/s41596-025-01316-8This protocol isolates lignin from various types of biomass, screens for high-quality lignins, and uses them to directly prepare lignin adhesives without chemical modification, the performance of which is then assessed. It produces lignin adhesives with light colors and superior properties.
dlvr.it
January 23, 2026 at 3:56 AM
New Article! Screening high-quality lignins extracted from biomass to prepare biobased wood adhesives
New Article! Transsynaptic tracing techniques to interrogate neuronal connectivity of glioblastomas
Transsynaptic tracing techniques to interrogate neuronal connectivity of glioblastomas
Nature Protocols, Published online: 21 January 2026; doi:10.1038/s41596-025-01287-wThis protocol details transsynaptic tracing approaches using retrograde and anterograde viral tracers to map and manipulate neuron–tumor circuits in xenografts, brain organoid models and co-cultures.
dlvr.it
January 21, 2026 at 3:01 PM
New Article! Transsynaptic tracing techniques to interrogate neuronal connectivity of glioblastomas
New Article! Electrocatalytic C‒N bond construction from inorganic nitrogen sources in water
Electrocatalytic C‒N bond construction from inorganic nitrogen sources in water
Nature Protocols, Published online: 20 January 2026; doi:10.1038/s41596-025-01298-7Urea, formamide, cyclohexanone oxime and amino acids are vital raw materials. Their synthesis often requires high temperatures and dangerous reagents. This protocol describes an aqueous electrocatalytic approach using inorganic nitrogen sources.
dlvr.it
January 20, 2026 at 12:58 PM
New Article! Electrocatalytic C‒N bond construction from inorganic nitrogen sources in water
New Article! Standardized protocols for analyzing lipocartilage and lipochondrocytes
Standardized protocols for analyzing lipocartilage and lipochondrocytes
Nature Protocols, Published online: 19 January 2026; doi:10.1038/s41596-025-01324-8Lipocartilage has been identified as a novel adipose-related cartilage subtype generated by lipochondrocytes, which produce intracellular lipid vacuoles that provide structural rigidity to the outer ear. Now, a protocol for reproducible isolation of lipocartilage and lipochondrocytes in mice is reported.
dlvr.it
January 20, 2026 at 2:28 AM
New Article! Standardized protocols for analyzing lipocartilage and lipochondrocytes
New Article! A comprehensive framework for statistical testing of brain dynamics
A comprehensive framework for statistical testing of brain dynamics
Nature Protocols, Published online: 19 January 2026; doi:10.1038/s41596-025-01300-2This protocol covers a package for running statistical testing on the temporal dynamics of neural activity data obtained from various functional neural recording modalities.
dlvr.it
January 20, 2026 at 2:28 AM
New Article! A comprehensive framework for statistical testing of brain dynamics
New Article! GPSeq maps the radial organization of eukaryotic genomes along the nuclear periphery–center axis
GPSeq maps the radial organization of eukaryotic genomes along the nuclear periphery–center axis
Nature Protocols, Published online: 19 January 2026; doi:10.1038/s41596-025-01278-xGPSeq is a genome-wide method for probing the radial organization of the genome in cells or nuclei by progressive in situ digestion of chromatin with a restriction enzyme diffusing inward from the periphery followed by high-throughput sequencing.
dlvr.it
January 20, 2026 at 2:28 AM
New Article! GPSeq maps the radial organization of eukaryotic genomes along the nuclear periphery–center axis
New Article! Isolation, purification and characterization of lipocartilage in mice
Isolation, purification and characterization of lipocartilage in mice
Nature Protocols, Published online: 19 January 2026; doi:10.1038/s41596-025-01302-0Here Plikus and colleagues provide a step-by-step protocol for the isolation and characterization of lipocartilage from mouse ear and the purification of its lipochondrocytes.
dlvr.it
January 20, 2026 at 2:28 AM
New Article! Isolation, purification and characterization of lipocartilage in mice
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January 19, 2026 at 11:05 AM
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Reposted by Nature Protocols
We’re thrilled to share our latest iTP-seq protocol for mapping bacterial translation landscapes in vitro — with a complete experimental workflow streamlined by Mélanie Gillard and an open source Python library for data analysis developed by Thibaud Renault.
January 17, 2026 at 2:22 PM
We’re thrilled to share our latest iTP-seq protocol for mapping bacterial translation landscapes in vitro — with a complete experimental workflow streamlined by Mélanie Gillard and an open source Python library for data analysis developed by Thibaud Renault.