Nature Protocols, Published online: 18 November 2025; doi:10.1038/s41596-025-01273-2Reproducible characterization of materials designed for radiative cooling is important for their development and application. The protocol describes how to acquire optical data and how to build and use outdoor and indoor thermal testing platforms.

Nature Protocols, Published online: 14 November 2025; doi:10.1038/s41596-025-01276-zThis Protocol describes preprocessing of different environmental samples and extraction of microplastics and nanoplastics, as well as qualitative and quantitative characterization of microplastics and nanoplastics. The analytical technologies used for characterization include attenuated total reflection-Fourier transform infrared spectroscopy, laser direct infrared spectroscopy and optical photothermal infrared microspectroscopy.

Job Title: Locum Associate or Senior Editor, Nature Reviews Locations: Heidelberg or New York - hybrid working model Application Deadline: 26th November 2025 – candidates will be considered as they ap...

Nature Protocols, Published online: 11 November 2025; doi:10.1038/s41596-025-01283-0This protocol describes the synthesis of macrocyclic macroPROTAC1 and trivalent PROTAC SIM1, detailing the generation of the macrocyclic and trivalent cores and their conjugation to the respective ligands, for enhanced target protein degradation.

Clear methods reporting is key for reliable and reproducible science and can also prevent an extended review process. We highlight Methods section requirements for a more efficient publication process...

Communications AI & Computing will be a broad journal covering applied computer science with a focus on the development of artificial intelligence methods and ...

Nature Protocols, Published online: 30 October 2025; doi:10.1038/s41596-025-01268-zWe provide a protocol for generating human pluripotent stem cell-derived blood-generating heart-forming organoids, a multitissue model encompassing aspects of human cardiac, endothelial and hematopoietic co-development, and techniques to stain and clear these large samples for laser microscopy.

The NFDI4Chem supports chemists to collect, store, process, publish and re-use research data, so that their data meet the FAIR conditions.

Nature Protocols, Published online: 28 October 2025; doi:10.1038/s41596-025-01253-6Slippery covalently attached liquid surfaces are useful in many applications that require low-static friction to droplets. This review compares six prominent methods for preparing polydimethylsiloxane bound to silica surfaces and highlights pitfalls and time savers.

Nature Protocols, Published online: 16 October 2025; doi:10.1038/s41596-025-01257-2ANPELA is a software package to compare and assess the performance of different workflows for processing single-cell proteomic data, ensuring the user selects the most appropriate processing workflow for their experimental design question.

Nature Protocols, Published online: 15 October 2025; doi:10.1038/s41596-025-01258-1The preparation of multicomponent viscoelastic self-enhancing sono-inks, synthesized as phase-transition reversible acoustic absorbers, enables acoustic volumetric printing beneath diverse tissue types in optically scattering media.

Nature Protocols, Published online: 13 October 2025; doi:10.1038/s41596-025-01255-4In mass photometry, the optical contrast generated by individual molecules at a glass–water interface enables mass-resolved quantification of biomolecular mixtures. This protocol describes how to optimize and validate this method.

Effectively engaging with large language models is becoming increasingly vital as they proliferate across research landscapes. This Comment presents a practical guide for understanding their capabilities and limitations, along with strategies for crafting well-structured queries, to extract maximum utility from these artificial intelligence tools.

Nature Protocols, Published online: 09 October 2025; doi:10.1038/s41596-025-01265-2Protocol for fabricating synthetic viscoelastic antigen-presenting cells and their application in T cell engineering. These synthetic cells support robust T cell activation and expansion and improve chimeric antigen receptor transduction efficiency.

Nature Protocols, Published online: 09 October 2025; doi:10.1038/s41596-025-01256-3Filter-aided expansion proteomics facilitates spatial proteomics at subcellular resolution by integrating tissue expansion, imaging-guided microdissection and filter-aided in-gel digestion. This combination enhances the resolution, throughput and reproducibility for data-independent acquisition-based mass spectrometry analysis.

Nature Protocols, Published online: 08 October 2025; doi:10.1038/s41596-025-01269-yThe complex spatiotemporal signaling of human neural tube development is successfully engineered in a device using microfluidic gradients, creating a high-fidelity model of the developing human central nervous system.

Nature Protocols, Published online: 08 October 2025; doi:10.1038/s41596-025-01252-7CRISPR screens are widely used to identify essential genes and potential drug targets, but typical setups were designed for the study of highly proliferative cancer cell lines. Now, a protocol, using inducible Cas9, enables screens of non-proliferative cell states including senescence, quiescence and terminal differentiation, thereby expanding the applicability of CRISPR screens.

Nature Protocols, Published online: 08 October 2025; doi:10.1038/s41596-025-01251-8Here, the authors provide a platform for inducible CRISPR–Cas9 screening to interrogate non-proliferative cellular states.

Nature Protocols, Published online: 08 October 2025; doi:10.1038/s41596-025-01266-1Protocol for generating spatially patterned, human neural tube- and forebrain-like structures, by using a microfluidic device to impose orthogonal and independent chemical gradients on human pluripotent stem cells.

Nature Protocols, Published online: 08 October 2025; doi:10.1038/s41596-025-01250-9The simultaneous recording of fast-scan cyclic voltammetry and functional magnetic resonance imaging signals enable the coupling of local tissue oxygen and neurotransmitter dynamics for measuring changes in the brain

Nature Protocols - MOF-303 is a promising water-harvesting sorbent that can take up water at low relative humidity and release it under mild heating. This metal–organic framework can be made...