Ryan Fink
@finkryan.bsky.social
PhD Student - Universität Bremen
and Universität zu Köln @massecklab.bsky.social
Check out our new calcium sensor PinkyCaMP: doi.org/10.1101/2024.12.16.628673
Now on Addgene!
and Universität zu Köln @massecklab.bsky.social
Check out our new calcium sensor PinkyCaMP: doi.org/10.1101/2024.12.16.628673
Now on Addgene!
Reposted by Ryan Fink
Fresh batch of Hot Plasmids here! Find new tools for:
🧠 Neural cell-surface proteomics
🔋 Nerve-to-cancer mitochondrial transfer @greletlab.bsky.social
🔬 Red calcium biosensors @massecklab.bsky.social & @piatkevich.bsky.social
✂️ CRISPRa-AAV for adipocytes @nadavahituv.bsky.social
& more!
🧠 Neural cell-surface proteomics
🔋 Nerve-to-cancer mitochondrial transfer @greletlab.bsky.social
🔬 Red calcium biosensors @massecklab.bsky.social & @piatkevich.bsky.social
✂️ CRISPRa-AAV for adipocytes @nadavahituv.bsky.social
& more!
November 11, 2025 at 3:00 PM
Fresh batch of Hot Plasmids here! Find new tools for:
🧠 Neural cell-surface proteomics
🔋 Nerve-to-cancer mitochondrial transfer @greletlab.bsky.social
🔬 Red calcium biosensors @massecklab.bsky.social & @piatkevich.bsky.social
✂️ CRISPRa-AAV for adipocytes @nadavahituv.bsky.social
& more!
🧠 Neural cell-surface proteomics
🔋 Nerve-to-cancer mitochondrial transfer @greletlab.bsky.social
🔬 Red calcium biosensors @massecklab.bsky.social & @piatkevich.bsky.social
✂️ CRISPRa-AAV for adipocytes @nadavahituv.bsky.social
& more!
Reposted by Ryan Fink
What happens when you combine 10 years of brain data with one of the world’s fastest supercomputers?
A virtual mouse cortex simulation, thanks to a global collaboration.
🧠📈 https://alleninstitute.org/news/one-of-worlds-most-detailed-virtual-brain-simulations-is-changing-how-we-study-the-brain/
A virtual mouse cortex simulation, thanks to a global collaboration.
🧠📈 https://alleninstitute.org/news/one-of-worlds-most-detailed-virtual-brain-simulations-is-changing-how-we-study-the-brain/
November 17, 2025 at 7:26 PM
What happens when you combine 10 years of brain data with one of the world’s fastest supercomputers?
A virtual mouse cortex simulation, thanks to a global collaboration.
🧠📈 https://alleninstitute.org/news/one-of-worlds-most-detailed-virtual-brain-simulations-is-changing-how-we-study-the-brain/
A virtual mouse cortex simulation, thanks to a global collaboration.
🧠📈 https://alleninstitute.org/news/one-of-worlds-most-detailed-virtual-brain-simulations-is-changing-how-we-study-the-brain/
Reposted by Ryan Fink
mStayRose is published in JBC 🥳🎉
Congratulations @wsctt.bsky.social @sporemohan.bsky.social and team for the monumental effort to make a non-natural amino acid incorporating fluorescent protein accessible. It's based on mStayGold, bright, and photostable:
www.jbc.org/article/S002...
Congratulations @wsctt.bsky.social @sporemohan.bsky.social and team for the monumental effort to make a non-natural amino acid incorporating fluorescent protein accessible. It's based on mStayGold, bright, and photostable:
www.jbc.org/article/S002...
StayRose: a photostable StayGold derivative red-shifted by genetic code expansion
Photobleaching of fluorescent proteins often limits the acquisition of high-quality
images in microscopy. StayGold, a novel dimeric green fluorescent protein recently
monomerised through sequence engi...
www.jbc.org
October 22, 2025 at 4:58 PM
mStayRose is published in JBC 🥳🎉
Congratulations @wsctt.bsky.social @sporemohan.bsky.social and team for the monumental effort to make a non-natural amino acid incorporating fluorescent protein accessible. It's based on mStayGold, bright, and photostable:
www.jbc.org/article/S002...
Congratulations @wsctt.bsky.social @sporemohan.bsky.social and team for the monumental effort to make a non-natural amino acid incorporating fluorescent protein accessible. It's based on mStayGold, bright, and photostable:
www.jbc.org/article/S002...
Reposted by Ryan Fink
New week, new tool: Find our Protein Domain Designer tool to generate publication-ready protein domain diagrams here: domaindesigner.farnunglab.com
September 29, 2025 at 3:01 PM
New week, new tool: Find our Protein Domain Designer tool to generate publication-ready protein domain diagrams here: domaindesigner.farnunglab.com
Dying in the weather, but the views in Seoul are incredible
August 29, 2025 at 5:03 AM
Dying in the weather, but the views in Seoul are incredible
Very excited to be at the #KSBNS2025 in a few days!
I’ll be presenting a poster in the first session (P-097) about our mScarlet based sensor for calcium PinkyCaMP and our developing mScarlet and 5HT1A based serotonin sensor.
Stop by to talk and learn about this new generation of red sensors!
I’ll be presenting a poster in the first session (P-097) about our mScarlet based sensor for calcium PinkyCaMP and our developing mScarlet and 5HT1A based serotonin sensor.
Stop by to talk and learn about this new generation of red sensors!
August 21, 2025 at 12:59 PM
Very excited to be at the #KSBNS2025 in a few days!
I’ll be presenting a poster in the first session (P-097) about our mScarlet based sensor for calcium PinkyCaMP and our developing mScarlet and 5HT1A based serotonin sensor.
Stop by to talk and learn about this new generation of red sensors!
I’ll be presenting a poster in the first session (P-097) about our mScarlet based sensor for calcium PinkyCaMP and our developing mScarlet and 5HT1A based serotonin sensor.
Stop by to talk and learn about this new generation of red sensors!
Reposted by Ryan Fink
How many photons are in a GFP? — more than last year, and more than you thought. Here's a simple, cheap, and practical method to break a fundamental limit in fluorescence microscopy. But it only works in light sheet!
August 19, 2025 at 7:31 PM
How many photons are in a GFP? — more than last year, and more than you thought. Here's a simple, cheap, and practical method to break a fundamental limit in fluorescence microscopy. But it only works in light sheet!
Reposted by Ryan Fink
#OCaMP is now on bioRxiv and reagents are available on #Addgene! 🧪
A sensitive orange fluorescent calcium ion indicator for imaging neural activity
www.biorxiv.org/content/10.1...
A sensitive orange fluorescent calcium ion indicator for imaging neural activity
www.biorxiv.org/content/10.1...
A sensitive orange fluorescent calcium ion indicator for imaging neural activity
Genetically encoded calcium indicators (GECIs) are vital tools for fluorescence-based visualization of neuronal activity with high spatial and temporal resolution. However, current highest-performance...
www.biorxiv.org
July 31, 2025 at 7:34 PM
#OCaMP is now on bioRxiv and reagents are available on #Addgene! 🧪
A sensitive orange fluorescent calcium ion indicator for imaging neural activity
www.biorxiv.org/content/10.1...
A sensitive orange fluorescent calcium ion indicator for imaging neural activity
www.biorxiv.org/content/10.1...
Reposted by Ryan Fink
For those of you attending academic conferences in the U.S., after October 1, 2025 please note the following:
Tucked away in the 'One Big Beautiful Bill' is the requirement that all visitors to the US must pay $250 just to enter the country starting 1 October! For a family of 5 that's $1250! I doubt this will help America's crashing tourism business which is down 10% already since January
July 18, 2025 at 7:08 PM
For those of you attending academic conferences in the U.S., after October 1, 2025 please note the following:
Reposted by Ryan Fink
(1/3) Excited to introduce our new GRAB sensors for a series of steroid hormones! These tools enable real-time detection of steroid hormone dynamics in vivo🐭🧠. Happy to share these sensors and welcome any feedback! Please contact yulonglilab2018@gmail.com for information.
July 10, 2025 at 3:15 PM
(1/3) Excited to introduce our new GRAB sensors for a series of steroid hormones! These tools enable real-time detection of steroid hormone dynamics in vivo🐭🧠. Happy to share these sensors and welcome any feedback! Please contact yulonglilab2018@gmail.com for information.
Reposted by Ryan Fink
A @csisingapore.bsky.social team showed D-I-Tasser can outperform AlphaFold2/3, elevating high-accuracy protein structure & function predictions. We wonder if this moves us closer to resolving protein–protein complex structure prediction? @natbiotech.nature.com
www.nature.com/articles/s41...
www.nature.com/articles/s41...
July 1, 2025 at 2:15 PM
A @csisingapore.bsky.social team showed D-I-Tasser can outperform AlphaFold2/3, elevating high-accuracy protein structure & function predictions. We wonder if this moves us closer to resolving protein–protein complex structure prediction? @natbiotech.nature.com
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Ryan Fink
New plasmids available from the @massecklab.bsky.social! PinkyCaMP, a mScarlet-based calcium sensor, can be leveraged for its exceptional brightness, photostability, and multiplexing capabilities.
https://twp.ai/4iobS2
https://twp.ai/4iobS2
June 26, 2025 at 12:00 PM
New plasmids available from the @massecklab.bsky.social! PinkyCaMP, a mScarlet-based calcium sensor, can be leveraged for its exceptional brightness, photostability, and multiplexing capabilities.
https://twp.ai/4iobS2
https://twp.ai/4iobS2
Reposted by Ryan Fink
New tools! GreenPy and ApplePy are fluorescent biosensors for pyruvate with huge responses (20–40x in vitro) and a range of affinities (10s of μM to mM). These should be game changers for imaging of metabolism! Great work by Shosei Imai @sikmys.bsky.social and team 👏. www.biorxiv.org/content/10.1...
High-performance genetically-encoded green and red fluorescent biosensors for pyruvate
Pyruvate is the end-product of glycolysis and a central metabolite involved in many biochemical pathways. However, a lack of high-performance (i.e., Δ F / F > 10) single fluorescent protein (FP)-base...
www.biorxiv.org
April 19, 2025 at 6:42 AM
New tools! GreenPy and ApplePy are fluorescent biosensors for pyruvate with huge responses (20–40x in vitro) and a range of affinities (10s of μM to mM). These should be game changers for imaging of metabolism! Great work by Shosei Imai @sikmys.bsky.social and team 👏. www.biorxiv.org/content/10.1...
Reposted by Ryan Fink
mScarlet3-H (aka mYongHong) is on the cover of Nature Methods! All plasmids are available for free from WeKwikGene wekwikgene.wllsb.edu.cn/publications...
June 18, 2025 at 12:16 AM
mScarlet3-H (aka mYongHong) is on the cover of Nature Methods! All plasmids are available for free from WeKwikGene wekwikgene.wllsb.edu.cn/publications...
Reposted by Ryan Fink
New powerful article up in the New York Times on cancelled and delayed grants
www.nytimes.com/interactive/...
www.nytimes.com/interactive/...
Here Are the Nearly 2,500 Medical Research Grants Canceled or Delayed by Trump (Gift Article)
Some cuts have been starkly visible, but the country’s medical grant-making machinery has also radically transformed outside the public eye.
www.nytimes.com
June 4, 2025 at 3:01 PM
New powerful article up in the New York Times on cancelled and delayed grants
www.nytimes.com/interactive/...
www.nytimes.com/interactive/...
Reposted by Ryan Fink
Happy to see my Postdoc work out @natbiotech.nature.com. Using HaloTag and synthetic fluorophores instead of FPs enables us to shift the spectral properties of kinase activity reporters (KARs) to the far-red 🔬. Huge thanks to everyone involved @jinzhanglab.bsky.social!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Far-red chemigenetic kinase biosensors enable multiplexed and super-resolved imaging of signaling networks - Nature Biotechnology
Far-red kinase biosensors are applied to image kinase signaling networks at super resolution.
www.nature.com
April 21, 2025 at 9:28 AM
Happy to see my Postdoc work out @natbiotech.nature.com. Using HaloTag and synthetic fluorophores instead of FPs enables us to shift the spectral properties of kinase activity reporters (KARs) to the far-red 🔬. Huge thanks to everyone involved @jinzhanglab.bsky.social!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Ryan Fink
First PinkyCaMP plasmids are available via @addgene.bsky.social
www.addgene.org/browse/artic...
Cc @finkryan.bsky.social @massecklab.bsky.social
www.addgene.org/browse/artic...
Cc @finkryan.bsky.social @massecklab.bsky.social
Addgene: PinkyCaMP a mScarlet-based calcium sensor with exceptional brightness, photostability, and multiplexing capabilities.
www.addgene.org
February 25, 2025 at 8:12 PM
First PinkyCaMP plasmids are available via @addgene.bsky.social
www.addgene.org/browse/artic...
Cc @finkryan.bsky.social @massecklab.bsky.social
www.addgene.org/browse/artic...
Cc @finkryan.bsky.social @massecklab.bsky.social
Reposted by Ryan Fink
A highly photostable monomeric red fluorescent protein
www.researchsquare.com/article/rs-5...
www.researchsquare.com/article/rs-5...
January 6, 2025 at 9:03 AM
A highly photostable monomeric red fluorescent protein
www.researchsquare.com/article/rs-5...
www.researchsquare.com/article/rs-5...
Reposted by Ryan Fink
PinkyCaMP a mScarlet-based calcium sensor with exceptional brightness, photostability, and multiplexing capabilities https://www.biorxiv.org/content/10.1101/2024.12.16.628673v1
December 18, 2024 at 5:16 AM
PinkyCaMP a mScarlet-based calcium sensor with exceptional brightness, photostability, and multiplexing capabilities https://www.biorxiv.org/content/10.1101/2024.12.16.628673v1
Reposted by Ryan Fink
🎄PinkyCaMP- our christmas gift to you! 🎁: We are excited to present PinkyCaMP, the first mScarlet based calcium sensor with:
✨exceptional, brightness
✨phototability
✨and optogenetic compatability.
Check out our new preprint:
www.biorxiv.org/content/10.1...
✨exceptional, brightness
✨phototability
✨and optogenetic compatability.
Check out our new preprint:
www.biorxiv.org/content/10.1...
December 18, 2024 at 10:01 AM
🎄PinkyCaMP- our christmas gift to you! 🎁: We are excited to present PinkyCaMP, the first mScarlet based calcium sensor with:
✨exceptional, brightness
✨phototability
✨and optogenetic compatability.
Check out our new preprint:
www.biorxiv.org/content/10.1...
✨exceptional, brightness
✨phototability
✨and optogenetic compatability.
Check out our new preprint:
www.biorxiv.org/content/10.1...