Sam Collie
@collieman13.bsky.social
PhD student at the University of Michigan
He/Him || Studying Cell Signaling in Neutrophils || I like Tea, Climbing, and Science
He/Him || Studying Cell Signaling in Neutrophils || I like Tea, Climbing, and Science
Reposted by Sam Collie
So happy to announce our new preprint, “A geothermal amoeba sets a new upper temperature limit for eukaryotes.” We cultured a novel amoeba from Lassen Volcanic NP (CA, USA) that divides at 63°C (145°F) 🔥 - a new record for euk growth!
#protistsonsky 🧵
#protistsonsky 🧵
November 25, 2025 at 8:41 PM
So happy to announce our new preprint, “A geothermal amoeba sets a new upper temperature limit for eukaryotes.” We cultured a novel amoeba from Lassen Volcanic NP (CA, USA) that divides at 63°C (145°F) 🔥 - a new record for euk growth!
#protistsonsky 🧵
#protistsonsky 🧵
Reposted by Sam Collie
Hot off the press! Our latest paper led by @fernpizza.bsky.social, understanding how plasmids evolve inside cells. These small, self-replicating DNA circles live inside bacteria and carry antibiotic resistance genes, but also compete with one another to replicate. 1/
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Intracellular competition shapes plasmid population dynamics
From populations of multicellular organisms to selfish genetic elements, conflicts between levels of biological organization are central to evolution. Plasmids are extrachromosomal, self-replicating g...
www.science.org
November 20, 2025 at 9:42 PM
Hot off the press! Our latest paper led by @fernpizza.bsky.social, understanding how plasmids evolve inside cells. These small, self-replicating DNA circles live inside bacteria and carry antibiotic resistance genes, but also compete with one another to replicate. 1/
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Sam Collie
Success. Nearly 259 Foldscope deployed in hands of unsuspecting candy crazy kids. Halloween is always more colorful with the microscopes - specially in a few weeks they could look at the micro-fauna of the teeth with so much sugar consumption.
November 1, 2025 at 10:10 AM
Success. Nearly 259 Foldscope deployed in hands of unsuspecting candy crazy kids. Halloween is always more colorful with the microscopes - specially in a few weeks they could look at the micro-fauna of the teeth with so much sugar consumption.
Reposted by Sam Collie
🚨Our collaboration with @centriolelab.bsky.social & @gautamdey.bsky.social is out today in @cp-cell.bsky.social
We show that #Expansion #Microscopy is a broad-spectrum modality for Euks, enabling 3D phenotypic maps rooted to phylogeny.
#ProtistsOnSky #SciComm #SciSky
www.cell.com/cell/fulltex...
We show that #Expansion #Microscopy is a broad-spectrum modality for Euks, enabling 3D phenotypic maps rooted to phylogeny.
#ProtistsOnSky #SciComm #SciSky
www.cell.com/cell/fulltex...
October 31, 2025 at 2:42 PM
🚨Our collaboration with @centriolelab.bsky.social & @gautamdey.bsky.social is out today in @cp-cell.bsky.social
We show that #Expansion #Microscopy is a broad-spectrum modality for Euks, enabling 3D phenotypic maps rooted to phylogeny.
#ProtistsOnSky #SciComm #SciSky
www.cell.com/cell/fulltex...
We show that #Expansion #Microscopy is a broad-spectrum modality for Euks, enabling 3D phenotypic maps rooted to phylogeny.
#ProtistsOnSky #SciComm #SciSky
www.cell.com/cell/fulltex...
Great example of incredibly useful and relevant “negative” data
I freaking love that this team must have been like TIME TO PREPRINT
Prenatal Acetaminophen Exposure Does Not Disrupt Human Fetal Brain Development in Cortical Organoid Models
www.biorxiv.org/content/10.1...
Prenatal Acetaminophen Exposure Does Not Disrupt Human Fetal Brain Development in Cortical Organoid Models
www.biorxiv.org/content/10.1...
Prenatal Acetaminophen Exposure Does Not Disrupt Human Fetal Brain Development in Cortical Organoid Models
Acetaminophen (APAP) is the most widely used analgesic during pregnancy, yet epidemiological studies have reported conflicting associations between prenatal exposure and neurodevelopmental disorders (...
www.biorxiv.org
October 9, 2025 at 2:40 PM
Great example of incredibly useful and relevant “negative” data
Reposted by Sam Collie
I freaking love that this team must have been like TIME TO PREPRINT
Prenatal Acetaminophen Exposure Does Not Disrupt Human Fetal Brain Development in Cortical Organoid Models
www.biorxiv.org/content/10.1...
Prenatal Acetaminophen Exposure Does Not Disrupt Human Fetal Brain Development in Cortical Organoid Models
www.biorxiv.org/content/10.1...
Prenatal Acetaminophen Exposure Does Not Disrupt Human Fetal Brain Development in Cortical Organoid Models
Acetaminophen (APAP) is the most widely used analgesic during pregnancy, yet epidemiological studies have reported conflicting associations between prenatal exposure and neurodevelopmental disorders (...
www.biorxiv.org
October 9, 2025 at 12:39 PM
I freaking love that this team must have been like TIME TO PREPRINT
Prenatal Acetaminophen Exposure Does Not Disrupt Human Fetal Brain Development in Cortical Organoid Models
www.biorxiv.org/content/10.1...
Prenatal Acetaminophen Exposure Does Not Disrupt Human Fetal Brain Development in Cortical Organoid Models
www.biorxiv.org/content/10.1...
This article by @pracheeac.bsky.social and @mbeisen.bsky.social sums up (in much stronger language) a lot of what I heard in a recent talk by Gary Ward (U of Vmt).
thescientistpapers.substack.com/p/how-to-be-...
thescientistpapers.substack.com/p/how-to-be-...
How to be a scientist in a post-journal world
When we created this site to write about things in science that need to be fixed, we chose not to start with publishing, even though it has been our preoccupation for decades.
thescientistpapers.substack.com
September 9, 2025 at 1:37 PM
This article by @pracheeac.bsky.social and @mbeisen.bsky.social sums up (in much stronger language) a lot of what I heard in a recent talk by Gary Ward (U of Vmt).
thescientistpapers.substack.com/p/how-to-be-...
thescientistpapers.substack.com/p/how-to-be-...
Really compelling argument to get rid of p-values in cell biology in favor of confidence intervals. Everytime I think about the state of statistics in biological research it makes me want to run, hide and become a statistician. rupress.org/jcb/article/...
Journal of Cell Biology | Rockefeller University Press
Journal of Cell Biology (JCB) publishes advances in any area of basic cell biology as well as applied cellular advances in fields such as immunology, neurobiology, metabolism, microbiology, developmen...
rupress.org
September 4, 2025 at 6:18 PM
Really compelling argument to get rid of p-values in cell biology in favor of confidence intervals. Everytime I think about the state of statistics in biological research it makes me want to run, hide and become a statistician. rupress.org/jcb/article/...
Reposted by Sam Collie
I'm not right wing but some of this "woke" stuff is a little over the top [6 months later] Slavery was fine. Your city is under martial law
August 20, 2025 at 4:52 PM
I'm not right wing but some of this "woke" stuff is a little over the top [6 months later] Slavery was fine. Your city is under martial law
Reposted by Sam Collie
Opinion | Don't get angry at ICE agents. They are just following orders.
by Adolf Eichmann
by Adolf Eichmann
July 23, 2025 at 3:16 AM
Opinion | Don't get angry at ICE agents. They are just following orders.
by Adolf Eichmann
by Adolf Eichmann
Cool new method for generating CAR-Macrophages In Vivo utilizing their phagocytosis of exogenous ennucleated stem cells!
www.pnas.org/doi/10.1073/...
www.pnas.org/doi/10.1073/...
In vivo generation of CAR macrophages via the enucleated mesenchymal stem cell delivery system for glioblastoma therapy | PNAS
Glioblastoma multiforme (GBM) is one of the most aggressive intracranial tumors for
which there is no effective treatment. Chimeric antigen recepto...
www.pnas.org
July 22, 2025 at 3:40 PM
Cool new method for generating CAR-Macrophages In Vivo utilizing their phagocytosis of exogenous ennucleated stem cells!
www.pnas.org/doi/10.1073/...
www.pnas.org/doi/10.1073/...
Reposted by Sam Collie
"Through AI Fluency, Ohio State students will be ‘bilingual’ — fluent in both their major field of study and the application of AI in that area"
Ohio State really not beating the allegations out of Ann Arbor here.
Ohio State really not beating the allegations out of Ann Arbor here.
Ohio State announces every student will use AI in class
COLUMBUS, Ohio (WCMH) — Starting this fall, every Ohio State student will be asked to use artificial intelligence. “Through AI Fluency, Ohio State students will be ‘bilingual’ — fluent …
www.nbc4i.com
June 8, 2025 at 10:15 PM
"Through AI Fluency, Ohio State students will be ‘bilingual’ — fluent in both their major field of study and the application of AI in that area"
Ohio State really not beating the allegations out of Ann Arbor here.
Ohio State really not beating the allegations out of Ann Arbor here.
Reposted by Sam Collie
Excited to share our recently published study on how DNA secretion in neutrophils modulates their inflammatory response spearheaded by @sbarya.bsky.social
Thanks to all our collaborators and contributors for their help getting this story to press.
👏👏
www.nature.com/articles/s41...
Thanks to all our collaborators and contributors for their help getting this story to press.
👏👏
www.nature.com/articles/s41...
Neutrophils secrete exosome-associated DNA to resolve sterile acute inflammation - Nature Cell Biology
Arya et al. report that migrating neutrophils resolve acute inflammation by releasing exosomes associated with nuclear DNA. This process is distinct from the release of neutrophil extracellular traps,...
www.nature.com
May 23, 2025 at 12:36 AM
Excited to share our recently published study on how DNA secretion in neutrophils modulates their inflammatory response spearheaded by @sbarya.bsky.social
Thanks to all our collaborators and contributors for their help getting this story to press.
👏👏
www.nature.com/articles/s41...
Thanks to all our collaborators and contributors for their help getting this story to press.
👏👏
www.nature.com/articles/s41...
Reposted by Sam Collie
Early for #MicroscopyMonday: #Hemifusomes- Newly discovered ESCRT-independent intermediates of intraluminal vesicle and multivesicular body formation! In collaboration with the Kachar Lab at the NIH and out today in @natcomms.nature.com : rdcu.be/emtQu
#cryoET #trafficking #organelle #hemifusion
#cryoET #trafficking #organelle #hemifusion
May 17, 2025 at 7:37 PM
Early for #MicroscopyMonday: #Hemifusomes- Newly discovered ESCRT-independent intermediates of intraluminal vesicle and multivesicular body formation! In collaboration with the Kachar Lab at the NIH and out today in @natcomms.nature.com : rdcu.be/emtQu
#cryoET #trafficking #organelle #hemifusion
#cryoET #trafficking #organelle #hemifusion
Reposted by Sam Collie
Pretty much everything is fucked, but here is some beautiful and exciting science from @archaeon-alex.bsky.social and colleagues.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Tissue-like multicellular development triggered by mechanical compression in archaea
The advent of clonal multicellularity is a critical evolutionary milestone, seen often in eukaryotes, rarely in bacteria, and only once in archaea. We show that uniaxial compression induces clonal mul...
www.science.org
April 4, 2025 at 3:40 AM
Pretty much everything is fucked, but here is some beautiful and exciting science from @archaeon-alex.bsky.social and colleagues.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Reposted by Sam Collie
Many brilliant scientists are now being punished by having our funding terminated because we are from underrepresented groups. We did everything right and we EARNED these awards. Remember us when you’re making decisions on whether DEI should be sacrificed to “protect” research funding. #InMyFeelings
Finally got the notice. Very sad day for the MOSAIC program that supported some of the most amazing scientists I know, as well as some of the awesome people on my lab.
April 2, 2025 at 9:53 PM
Many brilliant scientists are now being punished by having our funding terminated because we are from underrepresented groups. We did everything right and we EARNED these awards. Remember us when you’re making decisions on whether DEI should be sacrificed to “protect” research funding. #InMyFeelings
Incredible findings regarding the mechanism of active transport through Nuclear Pore Complexes. Seems importin travels only through the periphery of the central channel leaving a large dead zone in the center.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Overlapping nuclear import and export paths unveiled by two-colour MINFLUX - Nature
High spatiotemporal precision tracking using 3D MINFLUX shows that nuclear import and export occur in overlapping regions of the central pore, providing insight into transport across the nuclear pore ...
www.nature.com
March 28, 2025 at 3:43 PM
Incredible findings regarding the mechanism of active transport through Nuclear Pore Complexes. Seems importin travels only through the periphery of the central channel leaving a large dead zone in the center.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Sam Collie
www.nature.com/articles/s41...
I am amazed at this achievement. This is done using fairly off the shelf components. 🧪
I am amazed at this achievement. This is done using fairly off the shelf components. 🧪
Ångström-resolution fluorescence microscopy - Nature
The authors introduce a single-molecule DNA-barcoding method, resolution enhancement by sequential imaging, that improves the resolution of fluorescence microscopy down to the Ångström scale using off...
www.nature.com
November 28, 2024 at 12:08 PM
www.nature.com/articles/s41...
I am amazed at this achievement. This is done using fairly off the shelf components. 🧪
I am amazed at this achievement. This is done using fairly off the shelf components. 🧪
Reposted by Sam Collie
Thank you so much to our donors so far! You’re helping to make DFB possible. There’s still time to donate! You can use the link in our bio to access the official DFB Giving Blueday page. #givingblueday
March 19, 2025 at 5:37 PM
Thank you so much to our donors so far! You’re helping to make DFB possible. There’s still time to donate! You can use the link in our bio to access the official DFB Giving Blueday page. #givingblueday
Reposted by Sam Collie
Happy Giving Blueday! We appreciate all you do to help us continue our missing and make developmental biology accessible to students across the country #givingblueday
You can support us at maizeraise.umich.edu/schools/Univ...
You can support us at maizeraise.umich.edu/schools/Univ...
Developing Future Biologists
Join me and make a gift to Giving Blueday 2025
maizeraise.umich.edu
March 19, 2025 at 12:28 PM
Happy Giving Blueday! We appreciate all you do to help us continue our missing and make developmental biology accessible to students across the country #givingblueday
You can support us at maizeraise.umich.edu/schools/Univ...
You can support us at maizeraise.umich.edu/schools/Univ...
Reposted by Sam Collie
Hear from current and previous instructors, some of our alumni and one of our faculty advisors about the importance of the work we are doing! Donate now to help support our mission.
March 19, 2025 at 1:43 PM
Hear from current and previous instructors, some of our alumni and one of our faculty advisors about the importance of the work we are doing! Donate now to help support our mission.
Fascinating science inspires incredible art. Check out the video, it’s one of the least clickbait things i’ve seen
#CellMigration meets #SciArt
Naomi Wee (naomiwee.com) did this great movie to explain how amoeboid migration works (also mesenchymal & cancer migration)
Wanna know what happens if this leukocyte chooses the wrong path? Watch this !
www.youtube.com/watch?v=mPwn... @focalplane.bsky.social #SciSky
Naomi Wee (naomiwee.com) did this great movie to explain how amoeboid migration works (also mesenchymal & cancer migration)
Wanna know what happens if this leukocyte chooses the wrong path? Watch this !
www.youtube.com/watch?v=mPwn... @focalplane.bsky.social #SciSky
January 13, 2025 at 7:14 PM
Fascinating science inspires incredible art. Check out the video, it’s one of the least clickbait things i’ve seen
Reposted by Sam Collie
Black Swan is my comfort movie
January 13, 2025 at 2:30 AM
Black Swan is my comfort movie
I had an amazing time at the Directed Cell Migration Gordon Research Seminar. Excited for the next phase of amazing science and community in beautiful Tuscany!!
January 12, 2025 at 4:01 PM
I had an amazing time at the Directed Cell Migration Gordon Research Seminar. Excited for the next phase of amazing science and community in beautiful Tuscany!!