Chancie T
@chanciet.bsky.social
Senior Scientist @ Nxera Pharma 🇬🇧
Lab alumni: @cpaulino.bsky.social & @membraneenzymology.bsky.social (Slotboom) @ RUG 🇳🇱 & @mitocarriers.bsky.social (Kunji) @ MRC MBU/Cambridge University 🇬🇧
#MembraneProteins, #Transporters, #GPCRs, #cryoEM, #SBDD
Lab alumni: @cpaulino.bsky.social & @membraneenzymology.bsky.social (Slotboom) @ RUG 🇳🇱 & @mitocarriers.bsky.social (Kunji) @ MRC MBU/Cambridge University 🇬🇧
#MembraneProteins, #Transporters, #GPCRs, #cryoEM, #SBDD
Reposted by Chancie T
Our review on the peculiar properties of mitochondrial carriers of the SLC25 family out: portlandpress.com/biochemj/art...
July 25, 2025 at 11:29 AM
Our review on the peculiar properties of mitochondrial carriers of the SLC25 family out: portlandpress.com/biochemj/art...
Reposted by Chancie T
Solène’s 4.5 years of postdoctoral work in our lab (she started just before the corona pandemic) has now been published. A heroic effort that revealed -at the single molecule level- that ATP drives the dissociation of an ECF transporter complex under turnover conditions. See rdcu.be/elTKc
Single-molecule visualization of ATP-induced dynamics of the subunit composition of an ECF transporter complex under turnover conditions
Nature Communications - The association and dissociation dynamics of the ECF transporter complex for vitamin B12 are visualized by single-molecule FRET, highlighting the original transport...
rdcu.be
May 13, 2025 at 7:30 PM
Solène’s 4.5 years of postdoctoral work in our lab (she started just before the corona pandemic) has now been published. A heroic effort that revealed -at the single molecule level- that ATP drives the dissociation of an ECF transporter complex under turnover conditions. See rdcu.be/elTKc
Reposted by Chancie T
A colder frontier in cryo-EM 🧪🔬
Chris Russo’s group, inc. Joshua Dickerson, adapted #cryoEM to work at liquid helium temperatures (13 kelvins), where every frame captured contains more information than the equivalent using liquid nitrogen (81 kelvins).
Read more: tinyurl.com/mwwcunkc
#LMBResearch
Chris Russo’s group, inc. Joshua Dickerson, adapted #cryoEM to work at liquid helium temperatures (13 kelvins), where every frame captured contains more information than the equivalent using liquid nitrogen (81 kelvins).
Read more: tinyurl.com/mwwcunkc
#LMBResearch
April 23, 2025 at 8:49 AM
A colder frontier in cryo-EM 🧪🔬
Chris Russo’s group, inc. Joshua Dickerson, adapted #cryoEM to work at liquid helium temperatures (13 kelvins), where every frame captured contains more information than the equivalent using liquid nitrogen (81 kelvins).
Read more: tinyurl.com/mwwcunkc
#LMBResearch
Chris Russo’s group, inc. Joshua Dickerson, adapted #cryoEM to work at liquid helium temperatures (13 kelvins), where every frame captured contains more information than the equivalent using liquid nitrogen (81 kelvins).
Read more: tinyurl.com/mwwcunkc
#LMBResearch
Reposted by Chancie T
Our paper is finally out: Molecular basis of pyruvate transport and inhibition of the human mitochondrial pyruvate carrier | Science Advances www.science.org/doi/10.1126/...
#mitochondria #cryo-EM
#mitochondria #cryo-EM
Molecular basis of pyruvate transport and inhibition of the human mitochondrial pyruvate carrier
The mitochondrial pyruvate carrier transport mechanism is ΔpH driven and is inhibited competitively by distinct compound classes.
www.science.org
April 18, 2025 at 6:16 PM
Our paper is finally out: Molecular basis of pyruvate transport and inhibition of the human mitochondrial pyruvate carrier | Science Advances www.science.org/doi/10.1126/...
#mitochondria #cryo-EM
#mitochondria #cryo-EM
Check out our new preprint on the bacterial secondary active #transporter SbmA.
Shared structural mechanisms of alternating access between the secondary peptide transporter SbmA and ABC transporters
SbmA is a membrane transporter from Escherichia coli that imports antimicrobial peptides. Although the protein is a secondary transporter that is energized by the proton gradient, it is structurally r...
dx.doi.org
February 4, 2025 at 9:03 PM
Check out our new preprint on the bacterial secondary active #transporter SbmA.
Reposted by Chancie T
The work was a collaboration with Nate Traaseth. Hope the method will help others solve structures of small membrane proteins in multiple conformations. The fusion strategy can be seen as a tribute to the late Ron Kaback, who attempted a similar approach with crystallizing the LacY 30 years ago.
A standardized method for determining MFS structures! Fantastic work by the Traaseth and @danengw.bsky.social labs
Looking forward to bringing this platform in-house!
doi.org/10.1038/s414...
Looking forward to bringing this platform in-house!
doi.org/10.1038/s414...
A fiducial-assisted strategy compatible with resolving small MFS transporter structures in multiple conformations using cryo-EM - Nature Communications
Small transporters pose a challenge for cryo-EM structure elucidation. Here, the authors tackled this limitation by rigidly fusing a fiducial marker to a transporter C-terminus, enabling multiple stru...
doi.org
January 6, 2025 at 10:13 PM
The work was a collaboration with Nate Traaseth. Hope the method will help others solve structures of small membrane proteins in multiple conformations. The fusion strategy can be seen as a tribute to the late Ron Kaback, who attempted a similar approach with crystallizing the LacY 30 years ago.
Reposted by Chancie T
At the heart of biology is the mantra: structure dictates function.Biological structures are also beautiful in their intricacy. David Goodsell brought this into public view. As he leaves "Molecule of the Month". here's a huge thank you for helping me see more clearly🧪1/2
cdn.rcsb.org/rcsb-pdb/gen...
cdn.rcsb.org/rcsb-pdb/gen...
January 6, 2025 at 5:07 PM
At the heart of biology is the mantra: structure dictates function.Biological structures are also beautiful in their intricacy. David Goodsell brought this into public view. As he leaves "Molecule of the Month". here's a huge thank you for helping me see more clearly🧪1/2
cdn.rcsb.org/rcsb-pdb/gen...
cdn.rcsb.org/rcsb-pdb/gen...
Reposted by Chancie T
Using 33 pathogenic variants of citrin we identify crucial elements of the carrier domain required for transport and show that the N-terminal domain is not involved in calcium regulation of transport, but causes a mitochondrial import defect, when mutated.
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Distinct roles for the domains of the mitochondrial aspartate/glutamate carrier citrin in organellar localization and substrate transport
Citrin, the mitochondrial aspartate/glutamate carrier isoform 2 (AGC2), is structurally and mechanistically the most complex SLC25 family member, beca…
www.sciencedirect.com
December 1, 2024 at 12:47 PM
Using 33 pathogenic variants of citrin we identify crucial elements of the carrier domain required for transport and show that the N-terminal domain is not involved in calcium regulation of transport, but causes a mitochondrial import defect, when mutated.
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Reposted by Chancie T
Our new paper is out! #mitochondria #transporters #adeninetranslocase #SLC25
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Membrane potential stimulates ADP import and ATP export by the mitochondrial ADP/ATP carrier due to its positively charged binding site
Reorientation of a positively charged binding site drives adenine nucleotide exchange in the presence of a membrane potential.
www.science.org
November 30, 2024 at 9:54 PM
Our new paper is out! #mitochondria #transporters #adeninetranslocase #SLC25
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...