Amazing work Oli and thanks for sharing.

Pleased to share that the 200 kV reconstructions in this paper have been released today:
1. www.emdataresource.org/EMD-50994
2. www.emdataresource.org/EMD-50995
3. www.emdataresource.org/EMD-50996
4. www.emdataresource.org/EMD-50997
5. www.emdataresource.org/EMD-50998

Instead of competing, the Slotboom @membraneenzymology.bsky.social @unigroningen.bsky.social and Beis labs @imperialcollegeldn.bsky.social teamed up for a more complete story.

We also used #EPR #PELDOR studies and #MDsimulations to propose how protonation affects the conformational ensemble and induces coformational changes allowing the movement of antimicrobial peptides and protons across the #membrane.

The conformational states resemble those found in primary active transporters of the #ABC superfamily, and our bioinformatic analysis shows that SbmA-like peptide transporters (SLiPTs) likely evolved from an ancestral ABC transporter.

We have captured occluded and inward-facing states by #cryoEM elucidating the conformational changes associated with proton binding and substrate translocation.

Could you add me too? Thanks.

I would like to be added as well. Thank you.