Matthieu Sainlos
@ch3w13.bsky.social
Chemical biology - Protein engineering -
Neuroscience
Neuroscience
Reposted by Matthieu Sainlos
Check out our newest work! This is a story on how to get selectivity in binders - both isoform and site selectivity. Read the paper or enjoy this brief Skytorial of what we did!
www.biorxiv.org/content/10.1...
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www.biorxiv.org/content/10.1...
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PANCS-spec-Binders: A system for rapidly discovering isoform- or epitope-specific binders
Proteins that bind to a target protein of interest, termed "binders," are essential components of biological research reagents and therapeutics. Target proteins present multiple binding surfaces with ...
www.biorxiv.org
November 19, 2025 at 6:10 PM
Check out our newest work! This is a story on how to get selectivity in binders - both isoform and site selectivity. Read the paper or enjoy this brief Skytorial of what we did!
www.biorxiv.org/content/10.1...
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www.biorxiv.org/content/10.1...
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Reposted by Matthieu Sainlos
New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
November 13, 2025 at 4:20 PM
New preprint 🥳! We made photoclickable HaloTag ligands to precisely control protein labeling on living cells. With it, we can do some cool multicolor stuff. Huge congrats to Franzi and all co-authors! Check it out 👇
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Matthieu Sainlos
Registration for THE chemical biology conference of 2026 is now open! EMBO ChemBio 2026 in Heidelberg
DeGrado, Arikin, Picotti (Keynotes). @lmkdassama.bsky.social @brianliau.bsky.social @rhodamine110.bsky.social @benlehner.bsky.social @alitavassoli.bsky.social
www.embl.org/about/info/c...
DeGrado, Arikin, Picotti (Keynotes). @lmkdassama.bsky.social @brianliau.bsky.social @rhodamine110.bsky.social @benlehner.bsky.social @alitavassoli.bsky.social
www.embl.org/about/info/c...
Chemical biology 2026
www.embl.org
November 11, 2025 at 5:46 PM
Registration for THE chemical biology conference of 2026 is now open! EMBO ChemBio 2026 in Heidelberg
DeGrado, Arikin, Picotti (Keynotes). @lmkdassama.bsky.social @brianliau.bsky.social @rhodamine110.bsky.social @benlehner.bsky.social @alitavassoli.bsky.social
www.embl.org/about/info/c...
DeGrado, Arikin, Picotti (Keynotes). @lmkdassama.bsky.social @brianliau.bsky.social @rhodamine110.bsky.social @benlehner.bsky.social @alitavassoli.bsky.social
www.embl.org/about/info/c...
Reposted by Matthieu Sainlos
A new, nerdy paper. We figured out (some) of the rules underlying cell-permeability of probes and designed ligands that light up, grab, and move proteins around. Awesome @hhmijanelia.bsky.social x @uwmadison.bsky.social x @stjuderesearch.bsky.social collaboration! www.pnas.org/doi/10.1073/...
PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
www.pnas.org
October 27, 2025 at 9:43 PM
A new, nerdy paper. We figured out (some) of the rules underlying cell-permeability of probes and designed ligands that light up, grab, and move proteins around. Awesome @hhmijanelia.bsky.social x @uwmadison.bsky.social x @stjuderesearch.bsky.social collaboration! www.pnas.org/doi/10.1073/...
Reposted by Matthieu Sainlos
I am thrilled to release ProteinDJ: a high-performance and modular protein design pipeline. Our open-source workflow incorporates #RFdiffusion, #ProteinMPNN, #FAMPNN, #AlphaFold2 and #Boltz-2. It is a fast, free, and fun way to design proteins (1/5)
doi.org/10.1101/2025.09.24.678028 #proteindesign
doi.org/10.1101/2025.09.24.678028 #proteindesign
September 28, 2025 at 9:16 PM
I am thrilled to release ProteinDJ: a high-performance and modular protein design pipeline. Our open-source workflow incorporates #RFdiffusion, #ProteinMPNN, #FAMPNN, #AlphaFold2 and #Boltz-2. It is a fast, free, and fun way to design proteins (1/5)
doi.org/10.1101/2025.09.24.678028 #proteindesign
doi.org/10.1101/2025.09.24.678028 #proteindesign
Reposted by Matthieu Sainlos
Paralog-specific intrabodies for PSD-93 and SAP102 expand the molecular toolkit to resolve excitatory synapse organization https://www.biorxiv.org/content/10.1101/2025.08.24.671450v1
August 26, 2025 at 12:46 AM
Paralog-specific intrabodies for PSD-93 and SAP102 expand the molecular toolkit to resolve excitatory synapse organization https://www.biorxiv.org/content/10.1101/2025.08.24.671450v1
Reposted by Matthieu Sainlos
Also check out the associated News & Views by Masayasu Taki and Masayoshi Nakamura highlighting the work from @kjohnsson.bsky.social lab on SNAP-tag2
www.nature.com/articles/s41...
www.nature.com/articles/s41...
July 8, 2025 at 4:02 PM
Also check out the associated News & Views by Masayasu Taki and Masayoshi Nakamura highlighting the work from @kjohnsson.bsky.social lab on SNAP-tag2
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Matthieu Sainlos
Finally out in Nature Chem Bio:
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
SNAP-tag2 for faster and brighter protein labeling - Nature Chemical Biology
SNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...
www.nature.com
July 3, 2025 at 7:45 PM
Finally out in Nature Chem Bio:
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
SNAP-tag2 for faster and brighter protein labeling
www.nature.com/articles/s41...
Thank you Steffi and Veselin.
Reposted by Matthieu Sainlos
We are thrilled to welcome Barbara Imperiali at #ChemBioParis2025 in Paris, October 6-9, 2025. Sign up here: chembioparis2025.com – Early bird ends June 10 with reduced fees for @icbschembio.bsky.social - euchems.eu - @eu-openscreen.bsky.social - @scf-chembio.bsky.social - #ChemBioEvent
May 1, 2025 at 11:19 AM
We are thrilled to welcome Barbara Imperiali at #ChemBioParis2025 in Paris, October 6-9, 2025. Sign up here: chembioparis2025.com – Early bird ends June 10 with reduced fees for @icbschembio.bsky.social - euchems.eu - @eu-openscreen.bsky.social - @scf-chembio.bsky.social - #ChemBioEvent
Reposted by Matthieu Sainlos
Employing engineered split inteins to replace the internal regions of target proteins in a single reaction. www.science.org/doi/10.1126/... #NBThighlight
Protein editing using a coordinated transposition reaction
Protein engineering through the ligation of polypeptide fragments has proven enormously powerful for studying biochemical processes. In general, this strategy necessitates a final protein-folding step...
www.science.org
April 4, 2025 at 1:17 PM
Employing engineered split inteins to replace the internal regions of target proteins in a single reaction. www.science.org/doi/10.1126/... #NBThighlight
Reposted by Matthieu Sainlos
Maglia and colleagues discuss advances in nanopore technology en route to single-molecule protein sequencing go.nature.com/3FL0g3k
rdcu.be/efeZR
rdcu.be/efeZR
Toward single-molecule protein sequencing using nanopores - Nature Biotechnology
Maglia and colleagues discuss advances in nanopore technology en route to single-molecule protein sequencing
go.nature.com
April 1, 2025 at 4:04 AM
Maglia and colleagues discuss advances in nanopore technology en route to single-molecule protein sequencing go.nature.com/3FL0g3k
rdcu.be/efeZR
rdcu.be/efeZR
Reposted by Matthieu Sainlos
Toward single-molecule protein sequencing using nanopores - @unigroningen.bsky.social go.nature.com/3FL0g3k
March 18, 2025 at 1:39 PM
Toward single-molecule protein sequencing using nanopores - @unigroningen.bsky.social go.nature.com/3FL0g3k
Reposted by Matthieu Sainlos
A genomically recoded E. coli with a single stop codon www.nature.com/articles/s41...
Engineering a genomically recoded organism with one stop codon - Nature
Ochre, a strain of Escherichia coli engineered to have a single stop codon, enables reassignment of four codons for non-degenerate functions, such as incorporation of non-standard amino acids into pro...
www.nature.com
February 6, 2025 at 1:55 PM
A genomically recoded E. coli with a single stop codon www.nature.com/articles/s41...
Reposted by Matthieu Sainlos
Continuous directed evolution of cyclic peptides in cells to inhibit protein aggregation. From Tina Wang et al. #chembio #ChemSky www.nature.com/articles/s41...
Rapid discovery of cyclic peptide protein aggregation inhibitors by continuous selection - Nature Chemical Biology
A platform for the continuous selection of protein aggregation inhibitors from genetically encoded cyclic peptide libraries in Escherichia coli was developed. This platform was used to discover cyclic...
www.nature.com
January 14, 2025 at 12:28 AM
Continuous directed evolution of cyclic peptides in cells to inhibit protein aggregation. From Tina Wang et al. #chembio #ChemSky www.nature.com/articles/s41...
Reposted by Matthieu Sainlos
Super excited to finally share PANCS-Binders: our group's decade-long quest to accelerate protein binder discovery.
TLDR: PANCS-binders is fast (2 days), cheap (pennies), has extremely high fidelity (low false positive and negatives), and high-throughput.
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TLDR: PANCS-binders is fast (2 days), cheap (pennies), has extremely high fidelity (low false positive and negatives), and high-throughput.
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While everyone esle has been concentrating on generative binder design, this group has demonstrated screening libraries in vivo! www.biorxiv.org/content/10.1...
#biosky
#biosky
High-throughput protein binder discovery by rapid in vivo selection
Proteins that selectively bind to a target of interest are foundational components of research pipelines, diagnostics, and therapeutics. Current immunization-based, display-based, and computational ap...
www.biorxiv.org
January 7, 2025 at 8:50 PM
Super excited to finally share PANCS-Binders: our group's decade-long quest to accelerate protein binder discovery.
TLDR: PANCS-binders is fast (2 days), cheap (pennies), has extremely high fidelity (low false positive and negatives), and high-throughput.
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TLDR: PANCS-binders is fast (2 days), cheap (pennies), has extremely high fidelity (low false positive and negatives), and high-throughput.
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