Brendon Seale
@brendonseale.bsky.social
Scientific Manager of the Proteomics Facility at the Network Biology Collaborative Centre of the Lunenfeld-Tanenbaum Research Institute at Sinai Health in Toronto Canada. Whew, what a mouth full! #TeamMassSpec
Reposted by Brendon Seale
🚨 Our #singlecell article is out! We describe the Chip-Tip label-free SCP workflow quantifying >5000 proteins in single HeLa cells. With high scalability, increased depth and throughput we can now envision large-scale LFQ-SCP biomedical studies!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Enhanced sensitivity and scalability with a Chip-Tip workflow enables deep single-cell proteomics - Nature Methods
Chip-Tip is a label-free quantification-based single-cell proteomics workflow for deep single-cell proteomics, which identifies over 5,000 proteins and 40,000 peptides in single HeLa cells.
www.nature.com
January 17, 2025 at 7:54 AM
🚨 Our #singlecell article is out! We describe the Chip-Tip label-free SCP workflow quantifying >5000 proteins in single HeLa cells. With high scalability, increased depth and throughput we can now envision large-scale LFQ-SCP biomedical studies!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Brendon Seale
Finally, an experienced MS group performed a proper comparison of MS plasma proteomics vs. Olink! Kudos to the Coon lab! Cannot wait to read all the details!
Nice review of plasma proteomics methods by Josh Coon and his group . However the perchloric acid treatment of plasma will lead to the formation of cysteic acid and methionine sulphone which is not mentioned for the DIA search. .https://www.biorxiv.org/content/10.1101/2025.01.08.632035v1
A Technical Evaluation of Plasma Proteomics Technologies
Plasma proteomic technologies are rapidly evolving and of critical importance to the field of biomedical research. Here we report a technical evaluation of six notable plasma proteomic technologies - ...
www.biorxiv.org
January 14, 2025 at 11:09 AM
Finally, an experienced MS group performed a proper comparison of MS plasma proteomics vs. Olink! Kudos to the Coon lab! Cannot wait to read all the details!
Reposted by Brendon Seale
News in Proteomics Research blog post | Boringest study of 2025 so far! 9,000 harmonized QC samples ran by a bunch of labs over 4 years! proteomicsnews.blogs...
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#proteomics #prot-other
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#proteomics #prot-other
Boringest study of 2025 so far! 9,000 harmonized QC samples ran by a bunch of labs over 4 years!
In case you're new here or my sarcasm doesn't translate well - this study is amazingly fantastic and probably very very very very boring. ...
proteomicsnews.blogspot.com
January 10, 2025 at 3:20 PM
News in Proteomics Research blog post | Boringest study of 2025 so far! 9,000 harmonized QC samples ran by a bunch of labs over 4 years! proteomicsnews.blogs...
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#proteomics #prot-other
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#proteomics #prot-other
Reposted by Brendon Seale
My starter packs:
Proteomics pack (no space left): go.bsky.app/HH7kqEh
ECR proteomics: go.bsky.app/Dp8PHX1
British proteomics: go.bsky.app/S4kiXYL
Single cell proteomics: go.bsky.app/KYdPDb8
IRR-Edinburgh: go.bsky.app/Mca9njQ
Neutrophils: go.bsky.app/34xev7n
Proteomics pack (no space left): go.bsky.app/HH7kqEh
ECR proteomics: go.bsky.app/Dp8PHX1
British proteomics: go.bsky.app/S4kiXYL
Single cell proteomics: go.bsky.app/KYdPDb8
IRR-Edinburgh: go.bsky.app/Mca9njQ
Neutrophils: go.bsky.app/34xev7n
December 9, 2024 at 11:32 AM
My starter packs:
Proteomics pack (no space left): go.bsky.app/HH7kqEh
ECR proteomics: go.bsky.app/Dp8PHX1
British proteomics: go.bsky.app/S4kiXYL
Single cell proteomics: go.bsky.app/KYdPDb8
IRR-Edinburgh: go.bsky.app/Mca9njQ
Neutrophils: go.bsky.app/34xev7n
Proteomics pack (no space left): go.bsky.app/HH7kqEh
ECR proteomics: go.bsky.app/Dp8PHX1
British proteomics: go.bsky.app/S4kiXYL
Single cell proteomics: go.bsky.app/KYdPDb8
IRR-Edinburgh: go.bsky.app/Mca9njQ
Neutrophils: go.bsky.app/34xev7n
Reposted by Brendon Seale
My proteomics resources on Bluesky:
Proteomics Starter Pack (full): go.bsky.app/HH7kqEh PSP converted into a List so more researchers can be added: (bsky.app/profile/did:...)
Early Career Researchers in Proteomics Pack: go.bsky.app/Dp8PHX1
Single Cell Proteomics Pack: go.bsky.app/KYdPDb8
Proteomics Starter Pack (full): go.bsky.app/HH7kqEh PSP converted into a List so more researchers can be added: (bsky.app/profile/did:...)
Early Career Researchers in Proteomics Pack: go.bsky.app/Dp8PHX1
Single Cell Proteomics Pack: go.bsky.app/KYdPDb8
November 20, 2024 at 2:24 PM
My proteomics resources on Bluesky:
Proteomics Starter Pack (full): go.bsky.app/HH7kqEh PSP converted into a List so more researchers can be added: (bsky.app/profile/did:...)
Early Career Researchers in Proteomics Pack: go.bsky.app/Dp8PHX1
Single Cell Proteomics Pack: go.bsky.app/KYdPDb8
Proteomics Starter Pack (full): go.bsky.app/HH7kqEh PSP converted into a List so more researchers can be added: (bsky.app/profile/did:...)
Early Career Researchers in Proteomics Pack: go.bsky.app/Dp8PHX1
Single Cell Proteomics Pack: go.bsky.app/KYdPDb8
Reposted by Brendon Seale
The NBCC and @gingraslab.bsky.social are hiring a junior programmer/data specialist to help update and advance our proteomics software. If you are motivated and ready to learn, come join our team. For more details and to apply: apply.interfolio.com/147660. #hiring #proteomics
Apply - Interfolio
{{$ctrl.$state.data.pageTitle}} - Apply - Interfolio
apply.interfolio.com
June 18, 2024 at 2:12 PM
The NBCC and @gingraslab.bsky.social are hiring a junior programmer/data specialist to help update and advance our proteomics software. If you are motivated and ready to learn, come join our team. For more details and to apply: apply.interfolio.com/147660. #hiring #proteomics
Reposted by Brendon Seale
Join us next Tuesday as NBCC's @brendonseale.bsky.social will be presenting at CNPN's virtual meeting highlighting the value of scientific platforms and the vital role they play keeping our facilities operational and at the leading edge.
CNSP Spotlight
April 30th, 2024 at 3PM EDT
Join us for this month’s joint seminar initiative with the Canadian Network of Scientific Platforms (CNSP). We will hear about existing scientific platforms and core proteomics facilities. Register for free today!
cnpn.ca/seminar/
April 30th, 2024 at 3PM EDT
Join us for this month’s joint seminar initiative with the Canadian Network of Scientific Platforms (CNSP). We will hear about existing scientific platforms and core proteomics facilities. Register for free today!
cnpn.ca/seminar/
April 26, 2024 at 9:05 PM
Join us next Tuesday as NBCC's @brendonseale.bsky.social will be presenting at CNPN's virtual meeting highlighting the value of scientific platforms and the vital role they play keeping our facilities operational and at the leading edge.
Reposted by Brendon Seale
After a conversation with Bruker application specialist, the consensus looks like this: Bruker PepSep columns thread. @htlau.bsky.social
March 6, 2024 at 9:26 PM
After a conversation with Bruker application specialist, the consensus looks like this: Bruker PepSep columns thread. @htlau.bsky.social
Reposted by Brendon Seale
Super excited to be hosting this event next week with @gingraslab.bsky.social and Sinai Health. Expert talks from @maccoss.bsky.social & Jesper Olsen. Register at t.co/TeuB5bBYl6 and find out why our MS manager @brendonseale.bsky.social loves this instrument.
January 26, 2024 at 1:21 PM
Super excited to be hosting this event next week with @gingraslab.bsky.social and Sinai Health. Expert talks from @maccoss.bsky.social & Jesper Olsen. Register at t.co/TeuB5bBYl6 and find out why our MS manager @brendonseale.bsky.social loves this instrument.
Hello #TeamMassSpec and BioID fans! We are searching for particular lots of Cytiva Sepharose-Streptavidin resin. Recent lots have been poorly compatible with MS. If you have anything to spare from lots 10326476, 10312459, 10269523 or 10296366 please get in contact!
January 9, 2024 at 3:16 PM
Hello #TeamMassSpec and BioID fans! We are searching for particular lots of Cytiva Sepharose-Streptavidin resin. Recent lots have been poorly compatible with MS. If you have anything to spare from lots 10326476, 10312459, 10269523 or 10296366 please get in contact!
Celebrating our new toy. Also the first time I've been quoted in a news release! #TeamMassSpec
Festivities start early for proteomics researchers at Sinai Health with Canada’s first Astral mass...
www.sinaihealth.ca
December 20, 2023 at 8:18 PM
Celebrating our new toy. Also the first time I've been quoted in a news release! #TeamMassSpec
It’s here! It’s running! It still needs to be completely integrated with its crazy server! But it’s here!
December 8, 2023 at 4:16 PM
It’s here! It’s running! It still needs to be completely integrated with its crazy server! But it’s here!
Reposted by Brendon Seale
Now this looks like a cool job...
Assistant Curator/Professor in Ancient Biomolecules
The American Museum of Natural History (AMNH) seeks an Assistant Curator in the area of Ancient Biomolecules. The successful candidate will be appointed in a tenure-track position as an Assistant Cura...
careers.amnh.org
November 10, 2023 at 6:56 PM
Now this looks like a cool job...
New Astral news: The recommended PC for running ProteomeDiscoverer w/CHIMERYS running from Ardia is almost as powerful as the Ardia server itself... for some reason...
November 7, 2023 at 6:36 PM
New Astral news: The recommended PC for running ProteomeDiscoverer w/CHIMERYS running from Ardia is almost as powerful as the Ardia server itself... for some reason...
Heads up #TeamMassSpec US/Canada potential Orbitrap Astral purchasers: If you get the top tier Ardia server you need 5 (!!) 240V 20 A outlets with the twisty plugs (NEMA L6-20). So get your electricians on speed dial!
October 16, 2023 at 5:35 PM
Heads up #TeamMassSpec US/Canada potential Orbitrap Astral purchasers: If you get the top tier Ardia server you need 5 (!!) 240V 20 A outlets with the twisty plugs (NEMA L6-20). So get your electricians on speed dial!
Hi #TeamMassSpec! Great to be here finally!
October 4, 2023 at 4:49 PM
Hi #TeamMassSpec! Great to be here finally!