Not sure of the gradient/flow you are using but at lower flow it can often help to go to a lower ID on the emitter. That's solved a few problems for us, but not as extreme as what you are seeing.

I’ve been told they are working on it… since we got ours in January 😕

Even just diluting the provided standards in your lysis buffer can be effective. I did that a few days ago with a 562 nm BCA assay... thought it was Thermo or Pierce, unsure now.

IonOpticks is my go-to for most things these days. And in terms of heater setup for Thermo instruments it's quite affordable.

Nope. It's on the bottom of the stack. It's a fairly compacted design.

That literally happened to us twenty minutes after the techs left!

The HESI “looked better for the photos”

There are for sure bugs to work out. Like how 1 in 10 FAIMS runs randomly don't record data.

That's my problem too and I always forget all the syntax etc. when doing so too!

Where are your issues lying? It's pretty easy to run it via FragPipe but most of the trouble with these kinds of experiments is in setting up controls etc.

This is true. There is an up-front cost for PaSER. Thankfully no subscription fees!

That’s what I thought… it’s very confusing!