Astral and TIMS-TOF Ultra users I would be interested in how many points across the peak you are getting from your DIA experiments. If you can share gradient length and points across the peak as I would like to compare with the small mol CRO methods. Quantitative proteomics needs some rigour I think

Just spent a week moving into a new house and totally exhausted. New house not so new though as built in 1576 so I now live in a Elizabethan farm house with very high oak beams. Just back at my desk organising some training and consulting so retirement is busy!!

Love this advert

I would hope the Scottish government will do something to put the Orange one in his place. Ban him from visiting his golf courses in Scotland . That’s how you treat a petulant child.

First official day of retirement. I can highly recommend it. Just waiting for the trout season to open next month.

Proud dad moment. cumberlandspatriots.com/news/2025/2/...

Nice thing about being retired is I can come to scotland to see the kids when I want. Also caught up with old colleagues at CRUK Scotland and MRCPPU in Dundee. Now off to Aberdeen.

I should really post science discussion here, but this was my son playing #2 Freed Hardmann in the NAIA last night

Less than one month until I retire from full time work. I am going to offer training and consultancy from early march 2025, so if you know of anyone who would like training on LCMS systems or consultancy on proteomics sample prep etc please mention my name.

Nice review of plasma proteomics methods by Josh Coon and his group . However the perchloric acid treatment of plasma will lead to the formation of cysteic acid and methionine sulphone which is not mentioned for the DIA search. .https://www.biorxiv.org/content/10.1101/2025.01.08.632035v1

I am doing HILIC of oligonucleotides ( I know!!) and the sodiation I am seeing must be coming from the LC even though it has been thoroughly washed. The ASO made up in ammonium acetate/acetonitrile/milliQ water shows no sign of sodiation when infused. Any ideas on how to reduce the sodiation?

Anyone else feeling cleansed after de-X-ing themselves. I certainly do. Keep up the good converastions here where the sky is blue.

I feel refreshed ; X account deleted.

Well I have been on here since ver a week now and what a pleasant place to be. Good scientific discussions and a distinct lack of musk self promotion. Long may it continue.

Turning lists into a biologically relevant paper can take a long time. Years back we had a kinase substrate in liver that didn’t change its biology. However 7 years later it was found to affect angiogenesis, so right substrate wrong tissue.

Having worked in a CRO for the past 20 months where accurate quant is everything. Proteomics is slowly moving this way, but the days of listomics are over. Getting accurate quant on proteins of genuine interest is the future. Don’t see many Internal standards being used either.

Planning for retirement is good. Looking to move the caravan close to the River Dee so fishing will be literally on my doorstep.

I have just joined my old twitter colleagues. Bit of news. I am retiring (well semi-retiring) to have more time for fishing. Full time work in a CRO not my cup of tea.