Nick Morrice
@pommie331.bsky.social
A bad day’s fishing is better than a good day’s work. Freshly retired , but worked for 40 years in protein biochemistry and proteomics, with PTM analysis being a speciality.
It was a Voyager and a very fine instrument . I have a paper with over 6500 citations using that wonderful instrument.
March 21, 2025 at 12:00 AM
It was a Voyager and a very fine instrument . I have a paper with over 6500 citations using that wonderful instrument.
28 years ago got a DE-STR ( Death Star) and started mass fingerprinting at the MRCPPU in Dundee . All backed up with the wealth of protein chemistry knowledge in Dundee.
March 20, 2025 at 10:37 PM
28 years ago got a DE-STR ( Death Star) and started mass fingerprinting at the MRCPPU in Dundee . All backed up with the wealth of protein chemistry knowledge in Dundee.
However very few can actually show you the raw MS2 spectrum so you can decide if the fragments selected are real or noise. The Swath microApp from Sciex does this for .wiff files and you can filter in or out a lot of interesting hits based on the quality of the MS2 spectrum.
March 19, 2025 at 7:04 AM
However very few can actually show you the raw MS2 spectrum so you can decide if the fragments selected are real or noise. The Swath microApp from Sciex does this for .wiff files and you can filter in or out a lot of interesting hits based on the quality of the MS2 spectrum.
Thanks Bini. That is very useful to know. I think that quantitative proteomics has a way to go but it’s getting there. Need to add internal standards to general workflows though.
March 18, 2025 at 9:17 PM
Thanks Bini. That is very useful to know. I think that quantitative proteomics has a way to go but it’s getting there. Need to add internal standards to general workflows though.
That's what I was guessing. In the CRO I was working for before I retired 14 points across the peak was the minimum required which is rarely seen in proteomic experiments. Will be interesting to see how quantitative proteomics evolves and matures.
March 18, 2025 at 6:24 PM
That's what I was guessing. In the CRO I was working for before I retired 14 points across the peak was the minimum required which is rarely seen in proteomic experiments. Will be interesting to see how quantitative proteomics evolves and matures.
John doing his best Winston Churchill impersonation .
February 25, 2025 at 8:39 PM
John doing his best Winston Churchill impersonation .
Enjoy Albert. Fantastic part of the world and a trip to Wye River is worth it for the koalas and king parrots.
February 9, 2025 at 10:35 AM
Enjoy Albert. Fantastic part of the world and a trip to Wye River is worth it for the koalas and king parrots.
Business as usual Matt. The orange one huffs and puffs but has to get both houses to approve any of his directives.
January 23, 2025 at 3:36 PM
Business as usual Matt. The orange one huffs and puffs but has to get both houses to approve any of his directives.
0.3mm i.d columns at 5-10ul/min were bulletproof unless it was a failed digest. Usually 2000-5000 injections before needing to be changed.
January 21, 2025 at 10:33 PM
0.3mm i.d columns at 5-10ul/min were bulletproof unless it was a failed digest. Usually 2000-5000 injections before needing to be changed.
I have said this for years Luke. Micro is the way ahead and has been for me since 2011.
January 21, 2025 at 4:47 PM
I have said this for years Luke. Micro is the way ahead and has been for me since 2011.
Lots of respect from me. Too many students and post docs use pre cast without ever knowing the ins and outs of SDS PAGE. Costs plenty too!!
January 19, 2025 at 9:04 AM
Lots of respect from me. Too many students and post docs use pre cast without ever knowing the ins and outs of SDS PAGE. Costs plenty too!!
Self poured or pre-cast gels? I remember those days back in the 80's.
January 19, 2025 at 8:01 AM
Self poured or pre-cast gels? I remember those days back in the 80's.
In CRO world a minimum of 14 points across the peak is required for accurate quantification. This means your MS has to be fast.
January 18, 2025 at 2:41 PM
In CRO world a minimum of 14 points across the peak is required for accurate quantification. This means your MS has to be fast.
I think we should mix and match. High in Fahrenheit and low in Celsius just to confuse people even more😉
January 18, 2025 at 9:28 AM
I think we should mix and match. High in Fahrenheit and low in Celsius just to confuse people even more😉
Thanks Mike. Looking forward to more fishing mixed in with some science.
January 15, 2025 at 9:33 PM
Thanks Mike. Looking forward to more fishing mixed in with some science.
It is a cool comparison and yes I think Olink is only human proteins. As pointed out there is a conflict of interests from the authors but I think the study is sound and should be discussed. That’s what this place is for.
January 15, 2025 at 9:35 AM
It is a cool comparison and yes I think Olink is only human proteins. As pointed out there is a conflict of interests from the authors but I think the study is sound and should be discussed. That’s what this place is for.