KI researchers have captured the first detailed molecular snapshots of human polynucleotide phosphorylase in action, revealing how this essential mitochondrial enzyme degrades RNA through an elegant base-flipping mechanism. Published in @narjournal.bsky.social 🧪 #mitochondria doi.org/10.1093/nar/...

📄 Loop-mediated regulation and base flipping drive RNA cleavage by human mitochondrial PNPase

✍🏼 @bmhallberg.bsky.social @hdas29.bsky.social

📔Nucleic Acid Research @narjournal.bsky.social

Messenger RNA is made in the nucleus before it is exported to the cytoplasm for translation. But how are only correctly made mRNAs chosen and remodeled in the nucleus for export?
Our new paper investigates the nuclear events leading to human mRNA export. www.nature.com/articles/s41.... (1/4)

📜 The Herpes simplex Origin Binding Protein: Mechanisms for sequence specific DNA binding and dimerization revealed by Cryo-EM

🧑‍🔬 @emilgustavsson.bsky.social , Kay Grünewald, Per Elias, @bmhallberg.bsky.social

📔 Nucleic Acids Research @narjournal.bsky.social

🔗 doi.org/10.1093/nar/...

This one is a bit of a departure from the usual and definitely a work in progress!

We found that by using ab initio reconstruction at very high res, in very small steps, we could crack some small structures that had eluded us - e.g. 39kDa iPKAc (EMPIAR-10252), below.

Read on for details... 1/x

So happy to see these developments! Particularly the structure of the 29 kDa heterodimer of hemoglobin! Well done Oli & co! Of course can’t overlook the importance of sample prep and data collection - credit to Mark Herzik and Mengyu Wu

Finally my first #cryoEM paper is out🧪🧬🧶

📜 Dynamics of the Herpes simplex virus DNA polymerase holoenzyme during DNA synthesis and proof-reading revealed by Cryo-EM

🧑‍🔬@emilgustavsson.bsky.social , Kay Grünewald, Per Elias, @bmhallberg.bsky.social

📔 Nucleic Acids Research

🔗 doi.org/10.1093/nar/...