(2/n) Previously, it was thought that all 'patterned 3D genome structure' is entirely lost in mitosis. Using High-Res RCMC, we unexpectedly find that not only do microcompartments form during mitosis, they are actually stronger in mitosis than in interphase.
www.nature.com/articles/s41...

(6/n) We used
- 3 techniques: MINFLUX, SPT, SRLCI
- 2 labels: Histone H2B (global, pioneered by @kazu-maeshima.bsky.social ) and Fbn2 locus (local, www.science.org/doi/full/10.... )
- 2 cell lines: human U2OS, mESCs
- 4 perturbations: Loop extrusion, Transcription, Histone Acetylation, Supercoiling

(2/n) Chromatin dynamics govern all nuclear processes w, DNA movement (e.g. E-P search, DNA repair).

Mean Squared Displacement (MSD) should follow power law: MSD ~ time^alpha

fBm simulation shows this: a~0.25 oversamples local environment, whereas Brownian motion a=1 explores more globally.