Anders Sejr Hansen
@andersshansen.bsky.social
Associate Professor at MIT BE : http://ashansenlab.com
Interested in understanding the relationship between 3D genome structure and function
Interested in understanding the relationship between 3D genome structure and function
Nice, they have such an elegant system to study chromosome conformation without nuclear import in their preprint www.biorxiv.org/content/10.1... and it was really nice to see we both came to similar findings and to be able to coordinate.
Interphase chromosome conformation is specified by distinct folding programs inherited via mitotic chromosomes or through the cytoplasm
Identity-specific interphase chromosome conformation must be re-established each time a cell divides. To understand how interphase folding is inherited, we developed an experimental approach that phys...
www.biorxiv.org
October 20, 2025 at 7:03 PM
Nice, they have such an elegant system to study chromosome conformation without nuclear import in their preprint www.biorxiv.org/content/10.1... and it was really nice to see we both came to similar findings and to be able to coordinate.
(8/8) Finally our preprint www.biorxiv.org/content/10.1... was co-submitted with the amazing work of @allanaschooley.bsky.social @jobdekker.bsky.social www.biorxiv.org/content/10.1... whose preprint should also be online soon and finds similar concepts in a different system. Thanks for coordinating!
Dynamics of microcompartment formation at the mitosis-to-G1 transition
As cells exit mitosis and enter G1, mitotic chromosomes decompact and transcription is reestablished. Previously, Hi-C studies showed that essentially all interphase 3D genome features including A/B-c...
www.biorxiv.org
October 20, 2025 at 7:00 PM
(8/8) Finally our preprint www.biorxiv.org/content/10.1... was co-submitted with the amazing work of @allanaschooley.bsky.social @jobdekker.bsky.social www.biorxiv.org/content/10.1... whose preprint should also be online soon and finds similar concepts in a different system. Thanks for coordinating!
(7/n) This was a wonderful team effort led by Viraat Goel, @irate-physicist.bsky.social @nicholas-aboreden.bsky.social James Jusuf in collab with Leonid Mirny and Gerd Blobel.
Thanks also to @dimitristypas.bsky.social @natsmb.nature.com and the reviewers for steering and improving the paper
Thanks also to @dimitristypas.bsky.social @natsmb.nature.com and the reviewers for steering and improving the paper
October 20, 2025 at 6:57 PM
(7/n) This was a wonderful team effort led by Viraat Goel, @irate-physicist.bsky.social @nicholas-aboreden.bsky.social James Jusuf in collab with Leonid Mirny and Gerd Blobel.
Thanks also to @dimitristypas.bsky.social @natsmb.nature.com and the reviewers for steering and improving the paper
Thanks also to @dimitristypas.bsky.social @natsmb.nature.com and the reviewers for steering and improving the paper
(6/n) Any cell change that changes chromosome compaction (e.g. mitosis, differentiation, mechanical, osmotic) would be expected to result in stronger A/B/micro-compartments without strongly affecting loop extrusion.
E.g. 2-fold compaction --> 6-fold MC dot change
www.nature.com/articles/s41...
E.g. 2-fold compaction --> 6-fold MC dot change
www.nature.com/articles/s41...
Dynamics of microcompartment formation at the mitosis-to-G1 transition - Nature Structural & Molecular Biology
Goel et al. produce high-resolution three-dimensional genome structure mapping from mitosis to G1 phase to show unseen interactions between enhancers and promoters in prometaphase. Polymer modeling in...
www.nature.com
October 20, 2025 at 6:55 PM
(6/n) Any cell change that changes chromosome compaction (e.g. mitosis, differentiation, mechanical, osmotic) would be expected to result in stronger A/B/micro-compartments without strongly affecting loop extrusion.
E.g. 2-fold compaction --> 6-fold MC dot change
www.nature.com/articles/s41...
E.g. 2-fold compaction --> 6-fold MC dot change
www.nature.com/articles/s41...
(5/n) We think mitotic microcompartments are largely driven by entropy
Going back to thermo, G = H - T*S
We typically think in terms of H (e.g. affinity), but S is equally important.
Prediction: all affinity-driven interactions (A/B-compartments, microcompartments) should be favored by compaction
Going back to thermo, G = H - T*S
We typically think in terms of H (e.g. affinity), but S is equally important.
Prediction: all affinity-driven interactions (A/B-compartments, microcompartments) should be favored by compaction
October 20, 2025 at 6:52 PM
(5/n) We think mitotic microcompartments are largely driven by entropy
Going back to thermo, G = H - T*S
We typically think in terms of H (e.g. affinity), but S is equally important.
Prediction: all affinity-driven interactions (A/B-compartments, microcompartments) should be favored by compaction
Going back to thermo, G = H - T*S
We typically think in terms of H (e.g. affinity), but S is equally important.
Prediction: all affinity-driven interactions (A/B-compartments, microcompartments) should be favored by compaction
(4/n) Why are microcompartments stronger in mitosis?
Simulations from @irate-physicist.bsky.social point to compaction.
Mitotic chr 2-3x more compacted --> lower configurational entropy --> lower entropic penalty for forming microcompartments --> microcompartment formation favored in mitosis!
Simulations from @irate-physicist.bsky.social point to compaction.
Mitotic chr 2-3x more compacted --> lower configurational entropy --> lower entropic penalty for forming microcompartments --> microcompartment formation favored in mitosis!
October 20, 2025 at 6:48 PM
(4/n) Why are microcompartments stronger in mitosis?
Simulations from @irate-physicist.bsky.social point to compaction.
Mitotic chr 2-3x more compacted --> lower configurational entropy --> lower entropic penalty for forming microcompartments --> microcompartment formation favored in mitosis!
Simulations from @irate-physicist.bsky.social point to compaction.
Mitotic chr 2-3x more compacted --> lower configurational entropy --> lower entropic penalty for forming microcompartments --> microcompartment formation favored in mitosis!
(3/n) Do mitotic microcompartments have a transcriptional function?
Prior work from @chrishsiung.bsky.social Gerd Blobel discovered 'mitotic transcriptional spiking', where ~half of genes transcriptionally spike in mitosis.
We now show that microcompartment dot number and strength predicts spiking
Prior work from @chrishsiung.bsky.social Gerd Blobel discovered 'mitotic transcriptional spiking', where ~half of genes transcriptionally spike in mitosis.
We now show that microcompartment dot number and strength predicts spiking
October 20, 2025 at 6:45 PM
(3/n) Do mitotic microcompartments have a transcriptional function?
Prior work from @chrishsiung.bsky.social Gerd Blobel discovered 'mitotic transcriptional spiking', where ~half of genes transcriptionally spike in mitosis.
We now show that microcompartment dot number and strength predicts spiking
Prior work from @chrishsiung.bsky.social Gerd Blobel discovered 'mitotic transcriptional spiking', where ~half of genes transcriptionally spike in mitosis.
We now show that microcompartment dot number and strength predicts spiking
(2/n) Previously, it was thought that all 'patterned 3D genome structure' is entirely lost in mitosis. Using High-Res RCMC, we unexpectedly find that not only do microcompartments form during mitosis, they are actually stronger in mitosis than in interphase.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
October 20, 2025 at 6:41 PM
(2/n) Previously, it was thought that all 'patterned 3D genome structure' is entirely lost in mitosis. Using High-Res RCMC, we unexpectedly find that not only do microcompartments form during mitosis, they are actually stronger in mitosis than in interphase.
www.nature.com/articles/s41...
www.nature.com/articles/s41...