Alessandro Zunino
@zuninoale.bsky.social
Physicist interested in classical and quantum optics.
All-around climber and amateur alpinist.
https://alessandro-zunino.github.io/
All-around climber and amateur alpinist.
https://alessandro-zunino.github.io/
Pinned
Our last paper on s²ISM (super-resolution sectioning #ImageScanningMicroscopy) just got published on Nature Photonics @natphoton.nature.com! Discover how structured detection in laser scanning microscopes enhances both resolution and optical sectioning:
www.nature.com/articles/s41...
🧵1/7
www.nature.com/articles/s41...
🧵1/7
Reposted by Alessandro Zunino
#OPENACCESS: Variational calculus approach to Zernike polynomials with application to FCS. Ivan Gligonov and Jörg Enderlein.
Variational calculus approach to Zernike polynomials with application to FCS
Zernike polynomials are a sequence of orthogonal polynomials that play a crucial role in optics and, in particular, modeling microscopy systems. Introduced by Frits Zernike in 1934, they are…
www.cell.com
October 24, 2025 at 2:04 PM
#OPENACCESS: Variational calculus approach to Zernike polynomials with application to FCS. Ivan Gligonov and Jörg Enderlein.
Reposted by Alessandro Zunino
@vicidominilab.bsky.social's lecture is online !
👉 “Photon-Resolved Microscopy : Image-Scanning Microscopy with SPAD Array Detector and Beyond”
👉 youtu.be/Pgn-8F87RUE
@iitalk.bsky.social @cnrsingenierie.bsky.social
#SPADarray #BrightEyes_ERC #photonresolvedmicroscopy #imagescanningmicroscopy
👉 “Photon-Resolved Microscopy : Image-Scanning Microscopy with SPAD Array Detector and Beyond”
👉 youtu.be/Pgn-8F87RUE
@iitalk.bsky.social @cnrsingenierie.bsky.social
#SPADarray #BrightEyes_ERC #photonresolvedmicroscopy #imagescanningmicroscopy
October 30, 2025 at 8:32 PM
@vicidominilab.bsky.social's lecture is online !
👉 “Photon-Resolved Microscopy : Image-Scanning Microscopy with SPAD Array Detector and Beyond”
👉 youtu.be/Pgn-8F87RUE
@iitalk.bsky.social @cnrsingenierie.bsky.social
#SPADarray #BrightEyes_ERC #photonresolvedmicroscopy #imagescanningmicroscopy
👉 “Photon-Resolved Microscopy : Image-Scanning Microscopy with SPAD Array Detector and Beyond”
👉 youtu.be/Pgn-8F87RUE
@iitalk.bsky.social @cnrsingenierie.bsky.social
#SPADarray #BrightEyes_ERC #photonresolvedmicroscopy #imagescanningmicroscopy
Reposted by Alessandro Zunino
Andrea Bucci and Laurent Le will run two workshops on building a custom #PhotonResolvedMicroscopy system and generating great #ImageScanningMicroscopy images.
Thanks @iitalk.bsky.social @erc.europa.eu @genoainstruments.bsky.social and #PNRR RNA&GeneTheraphy for support. See you at #Mifobio2025
Thanks @iitalk.bsky.social @erc.europa.eu @genoainstruments.bsky.social and #PNRR RNA&GeneTheraphy for support. See you at #Mifobio2025
October 6, 2025 at 4:39 PM
Andrea Bucci and Laurent Le will run two workshops on building a custom #PhotonResolvedMicroscopy system and generating great #ImageScanningMicroscopy images.
Thanks @iitalk.bsky.social @erc.europa.eu @genoainstruments.bsky.social and #PNRR RNA&GeneTheraphy for support. See you at #Mifobio2025
Thanks @iitalk.bsky.social @erc.europa.eu @genoainstruments.bsky.social and #PNRR RNA&GeneTheraphy for support. See you at #Mifobio2025
Reposted by Alessandro Zunino
#Mifobio2025
Focus on the module 2 :
👉 the challenges of spatial and temporal quantification
We are delighted to welcome Dr. Giuseppe VICIDOMINI @vicidominilab.bsky.social @iitalk.bsky.social
Lecture - “Photon-Resolved Microscopy : Image-Scanning Microscopy with SPAD Array Detector and Beyond”
Focus on the module 2 :
👉 the challenges of spatial and temporal quantification
We are delighted to welcome Dr. Giuseppe VICIDOMINI @vicidominilab.bsky.social @iitalk.bsky.social
Lecture - “Photon-Resolved Microscopy : Image-Scanning Microscopy with SPAD Array Detector and Beyond”
October 4, 2025 at 4:12 PM
#Mifobio2025
Focus on the module 2 :
👉 the challenges of spatial and temporal quantification
We are delighted to welcome Dr. Giuseppe VICIDOMINI @vicidominilab.bsky.social @iitalk.bsky.social
Lecture - “Photon-Resolved Microscopy : Image-Scanning Microscopy with SPAD Array Detector and Beyond”
Focus on the module 2 :
👉 the challenges of spatial and temporal quantification
We are delighted to welcome Dr. Giuseppe VICIDOMINI @vicidominilab.bsky.social @iitalk.bsky.social
Lecture - “Photon-Resolved Microscopy : Image-Scanning Microscopy with SPAD Array Detector and Beyond”
Reposted by Alessandro Zunino
Happy to share this article with my views on @elislenders.bsky.social and @vicidominilab.bsky.social work and discussing current developments in single-molecule localization combining structured excitation and detection. So many exciting perspectives in the field!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Localization of single molecules with structured illumination and structured detection - Light: Science & Applications
Light: Science & Applications - Localization of single molecules with structured illumination and structured detection
www.nature.com
September 30, 2025 at 6:17 PM
Happy to share this article with my views on @elislenders.bsky.social and @vicidominilab.bsky.social work and discussing current developments in single-molecule localization combining structured excitation and detection. So many exciting perspectives in the field!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Alessandro Zunino
Very happy to be in #ThePhotonics100 list for 2026. Big thanks to everyone in @vicidominilab.bsky.social and to all collaborators for their continuous support!
September 27, 2025 at 4:13 PM
Very happy to be in #ThePhotonics100 list for 2026. Big thanks to everyone in @vicidominilab.bsky.social and to all collaborators for their continuous support!
Reposted by Alessandro Zunino
Interferometric Image Scanning Microscopy for label-free imaging at 120 nm resolution inside live cells https://www.biorxiv.org/content/10.1101/2025.09.19.677416v1
September 21, 2025 at 3:48 PM
Interferometric Image Scanning Microscopy for label-free imaging at 120 nm resolution inside live cells https://www.biorxiv.org/content/10.1101/2025.09.19.677416v1
Reposted by Alessandro Zunino
🏆🆕🇪🇺 @elislenders.bsky.social, researcher of the IIT Molecular Microscopy and Spectroscopy unit @vicidominilab.bsky.social , coordinated by Giuseppe Vicidomini, has been awarded an #ERCStG by the @erc.europa.eu
opentalk.iit.it/en/the-resea...
opentalk.iit.it/en/the-resea...
The researcher Eli Slenders awarded ERC grant to develop new microscopes to detect the speed and position of biomolecules in cells - Talk iit
Among the 30 winning projects that will be carried out in Italy, one has been awarded to Eli Slenders from the Istituto Italiano di Tecnologia in Genoa Building a new microscope capable of tracking mo...
opentalk.iit.it
September 4, 2025 at 10:27 AM
🏆🆕🇪🇺 @elislenders.bsky.social, researcher of the IIT Molecular Microscopy and Spectroscopy unit @vicidominilab.bsky.social , coordinated by Giuseppe Vicidomini, has been awarded an #ERCStG by the @erc.europa.eu
opentalk.iit.it/en/the-resea...
opentalk.iit.it/en/the-resea...
Reposted by Alessandro Zunino
#NewPaper on the blend of computational microscopy with chemical imaging: enabling high-speed quantitative CARS imaging via a new compressive Raman framework.
Kudos @spzhao0724.bsky.social
A collaboration between @lkblab.bsky.social and @ukmlv.bsky.social from Washington Uni St.Louis rdcu.be/eCjLz
Kudos @spzhao0724.bsky.social
A collaboration between @lkblab.bsky.social and @ukmlv.bsky.social from Washington Uni St.Louis rdcu.be/eCjLz
Computational field-resolved coherent chemical imaging
Nature Communications - A vibrational spectral phase retrieval approach to broadband CARS spectroscopy is presented, therefore enabling supervised compressive CARS microspectroscopy for...
rdcu.be
August 25, 2025 at 5:42 PM
#NewPaper on the blend of computational microscopy with chemical imaging: enabling high-speed quantitative CARS imaging via a new compressive Raman framework.
Kudos @spzhao0724.bsky.social
A collaboration between @lkblab.bsky.social and @ukmlv.bsky.social from Washington Uni St.Louis rdcu.be/eCjLz
Kudos @spzhao0724.bsky.social
A collaboration between @lkblab.bsky.social and @ukmlv.bsky.social from Washington Uni St.Louis rdcu.be/eCjLz
Reposted by Alessandro Zunino
Hello! Very excited to share our latest preprint, which is great news for us but terrible news for any diehard fans of PSNR and SSIM as image quality metrics in microscopy... (1/5)
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Image quality metrics fail to accurately represent biological information in fluorescence microscopy
Image processing methods offer the potential to improve the quality of fluorescence microscopy data, allowing for image acquisition at lower, less phototoxic illumination doses. The training and evalu...
www.biorxiv.org
August 7, 2025 at 1:00 PM
Hello! Very excited to share our latest preprint, which is great news for us but terrible news for any diehard fans of PSNR and SSIM as image quality metrics in microscopy... (1/5)
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Alessandro Zunino
and... our program for the upcoming IMAGE SCIENCE🔬 Gordon Research Conference (GRC) is 🎯LIVE🎯. It will take place April 26, 2026 in beautiful tuscany, italy 🇮🇹 🍕 🤌
Please check our stellar line-up, share, and register!
www.grc.org/image-scienc...
Please check our stellar line-up, share, and register!
www.grc.org/image-scienc...
2026 Image Science Conference GRC
The 2026 Gordon Research Conference on Image Science will be held in Lucca (Barga), Lucca Italy. Apply today to reserve your spot.
www.grc.org
August 18, 2025 at 12:56 PM
and... our program for the upcoming IMAGE SCIENCE🔬 Gordon Research Conference (GRC) is 🎯LIVE🎯. It will take place April 26, 2026 in beautiful tuscany, italy 🇮🇹 🍕 🤌
Please check our stellar line-up, share, and register!
www.grc.org/image-scienc...
Please check our stellar line-up, share, and register!
www.grc.org/image-scienc...
Reposted by Alessandro Zunino
"A #SPADarray in any laser-scanning microscope"
Here’s an interview with @zuninoale.bsky.social revealing the behind-the-scenes of our work @iitalk.bsky.social supported by @erc.europa.eu.
#BrightEyes_ERC, #photonresolvedmicroscopy #imagescanningmicroscopy
theanalyticalscientist.com/issues/2025/...
Here’s an interview with @zuninoale.bsky.social revealing the behind-the-scenes of our work @iitalk.bsky.social supported by @erc.europa.eu.
#BrightEyes_ERC, #photonresolvedmicroscopy #imagescanningmicroscopy
theanalyticalscientist.com/issues/2025/...
New Microscopy Method Tackles Thick Tissues
<p>Researchers at IIT have developed a breakthrough image scanning microscopy (ISM) method that enables both super-resolution and optical sectioning in thick biological tissues—without altering micros...
theanalyticalscientist.com
August 14, 2025 at 12:11 PM
"A #SPADarray in any laser-scanning microscope"
Here’s an interview with @zuninoale.bsky.social revealing the behind-the-scenes of our work @iitalk.bsky.social supported by @erc.europa.eu.
#BrightEyes_ERC, #photonresolvedmicroscopy #imagescanningmicroscopy
theanalyticalscientist.com/issues/2025/...
Here’s an interview with @zuninoale.bsky.social revealing the behind-the-scenes of our work @iitalk.bsky.social supported by @erc.europa.eu.
#BrightEyes_ERC, #photonresolvedmicroscopy #imagescanningmicroscopy
theanalyticalscientist.com/issues/2025/...
Reposted by Alessandro Zunino
New open access article online: Structured detection for simultaneous super-resolution and optical sectioning in laser scanning microscopy.
go.nature.com/4mfYqb6
go.nature.com/4mfYqb6
Structured detection for simultaneous super-resolution and optical sectioning in laser scanning microscopy - Nature Photonics
A reconstruction method for image scanning microscopy exploits all the information encoded in the four-dimensional image scanning microscopy dataset to achieve optical sectioning and maintain super-re...
go.nature.com
August 14, 2025 at 4:54 AM
New open access article online: Structured detection for simultaneous super-resolution and optical sectioning in laser scanning microscopy.
go.nature.com/4mfYqb6
go.nature.com/4mfYqb6
Reposted by Alessandro Zunino
A research team led by G. Vicidomini, PI of the IIT Molecular Microscopy and Spectroscopy Lab @vicidominilab.bsky.social , has published in #NaturePhotonics @nature.com a new imaging method that significantly enhances the observation of complex biological tissues.
opentalk.iit.it/en/a-new-mic...
opentalk.iit.it/en/a-new-mic...
A new microscopy technique to capture the complexity of biological tissues - Talk iit
The research was conducted by the team led by Giuseppe Vicidomini, with funding from the European Research Council (ERC) Until today, skin, brain, and all tissues of the human body were difficult to o...
opentalk.iit.it
July 24, 2025 at 10:48 AM
A research team led by G. Vicidomini, PI of the IIT Molecular Microscopy and Spectroscopy Lab @vicidominilab.bsky.social , has published in #NaturePhotonics @nature.com a new imaging method that significantly enhances the observation of complex biological tissues.
opentalk.iit.it/en/a-new-mic...
opentalk.iit.it/en/a-new-mic...
Reposted by Alessandro Zunino
“A #SPADarray in any laser scanning #microscope”. That is the latest effort from a group of people that shared this mantra. Proud of all of them.
@erc.europa.eu
#imagescanningmicroscopy #photonresolvedmicroscopy
#superresolution
#BrightEyes_ERC
doi.org/10.1038/s415...
@erc.europa.eu
#imagescanningmicroscopy #photonresolvedmicroscopy
#superresolution
#BrightEyes_ERC
doi.org/10.1038/s415...
July 24, 2025 at 12:11 PM
“A #SPADarray in any laser scanning #microscope”. That is the latest effort from a group of people that shared this mantra. Proud of all of them.
@erc.europa.eu
#imagescanningmicroscopy #photonresolvedmicroscopy
#superresolution
#BrightEyes_ERC
doi.org/10.1038/s415...
@erc.europa.eu
#imagescanningmicroscopy #photonresolvedmicroscopy
#superresolution
#BrightEyes_ERC
doi.org/10.1038/s415...
Reposted by Alessandro Zunino
#ISMFLUX shows #SPADarray is the future of #MINFLUX, improving localisation robustness and enabling the transformation of a #confocal #microscope into a #MINFLUX system.
www.nature.com/articles/s41...
#photonresolvedmicroscopy #BrightEyes_ERC
@iitalk.bsky.social @erc.europa.eu @ec.europa.eu
www.nature.com/articles/s41...
#photonresolvedmicroscopy #BrightEyes_ERC
@iitalk.bsky.social @erc.europa.eu @ec.europa.eu
July 4, 2025 at 1:18 PM
#ISMFLUX shows #SPADarray is the future of #MINFLUX, improving localisation robustness and enabling the transformation of a #confocal #microscope into a #MINFLUX system.
www.nature.com/articles/s41...
#photonresolvedmicroscopy #BrightEyes_ERC
@iitalk.bsky.social @erc.europa.eu @ec.europa.eu
www.nature.com/articles/s41...
#photonresolvedmicroscopy #BrightEyes_ERC
@iitalk.bsky.social @erc.europa.eu @ec.europa.eu
Reposted by Alessandro Zunino
Excited to share our paper on ISM-FLUX, our new #MINFLUX-based technology. Achieve sub-10 nm localization uncertainty on single molecules using a straightforward #confocal-based setup with an array detector. 1/6 www.nature.com/articles/s41...
Array detection enables large localization range for simple and robust MINFLUX - Light: Science & Applications
Light: Science & Applications - Array detection enables large localization range for simple and robust MINFLUX
www.nature.com
July 4, 2025 at 7:30 AM
Excited to share our paper on ISM-FLUX, our new #MINFLUX-based technology. Achieve sub-10 nm localization uncertainty on single molecules using a straightforward #confocal-based setup with an array detector. 1/6 www.nature.com/articles/s41...
Reposted by Alessandro Zunino
Alessandro Zunino now describes contributions to the Image Scanning Microscope (s^2-ISM) for super-res 3D sectioning much like SIM, and FLIM-capable but with a small confocal photodiode array!
June 30, 2025 at 3:29 PM
Alessandro Zunino now describes contributions to the Image Scanning Microscope (s^2-ISM) for super-res 3D sectioning much like SIM, and FLIM-capable but with a small confocal photodiode array!
Reposted by Alessandro Zunino
It is time to update our #photonresolvedmicroscope to make it more #smart, and we are looking for a PhD candidate. Here are the application details
vicidominilab.github.io/positions/
If you are interested, please get in touch with me.
@iitalk.bsky.social
#superresolution #microscopy #SPADarray
vicidominilab.github.io/positions/
If you are interested, please get in touch with me.
@iitalk.bsky.social
#superresolution #microscopy #SPADarray
June 17, 2025 at 8:13 AM
It is time to update our #photonresolvedmicroscope to make it more #smart, and we are looking for a PhD candidate. Here are the application details
vicidominilab.github.io/positions/
If you are interested, please get in touch with me.
@iitalk.bsky.social
#superresolution #microscopy #SPADarray
vicidominilab.github.io/positions/
If you are interested, please get in touch with me.
@iitalk.bsky.social
#superresolution #microscopy #SPADarray
Reposted by Alessandro Zunino
Our last paper on s²ISM (super-resolution sectioning #ImageScanningMicroscopy) just got published on Nature Photonics @natphoton.nature.com! Discover how structured detection in laser scanning microscopes enhances both resolution and optical sectioning:
www.nature.com/articles/s41...
🧵1/7
www.nature.com/articles/s41...
🧵1/7
June 6, 2025 at 5:40 AM
Our last paper on s²ISM (super-resolution sectioning #ImageScanningMicroscopy) just got published on Nature Photonics @natphoton.nature.com! Discover how structured detection in laser scanning microscopes enhances both resolution and optical sectioning:
www.nature.com/articles/s41...
🧵1/7
www.nature.com/articles/s41...
🧵1/7
Our last paper on s²ISM (super-resolution sectioning #ImageScanningMicroscopy) just got published on Nature Photonics @natphoton.nature.com! Discover how structured detection in laser scanning microscopes enhances both resolution and optical sectioning:
www.nature.com/articles/s41...
🧵1/7
www.nature.com/articles/s41...
🧵1/7
June 6, 2025 at 5:40 AM
Our last paper on s²ISM (super-resolution sectioning #ImageScanningMicroscopy) just got published on Nature Photonics @natphoton.nature.com! Discover how structured detection in laser scanning microscopes enhances both resolution and optical sectioning:
www.nature.com/articles/s41...
🧵1/7
www.nature.com/articles/s41...
🧵1/7
Reposted by Alessandro Zunino
#PhysicsFactlet
A Shack-Hartmann sensor is a simple and widely used device to measure the phase profile of a wavefront (aka "where the light is coming from").
A mini 🧵
1/
#Optics #Physics
A Shack-Hartmann sensor is a simple and widely used device to measure the phase profile of a wavefront (aka "where the light is coming from").
A mini 🧵
1/
#Optics #Physics
May 27, 2025 at 9:28 AM
#PhysicsFactlet
A Shack-Hartmann sensor is a simple and widely used device to measure the phase profile of a wavefront (aka "where the light is coming from").
A mini 🧵
1/
#Optics #Physics
A Shack-Hartmann sensor is a simple and widely used device to measure the phase profile of a wavefront (aka "where the light is coming from").
A mini 🧵
1/
#Optics #Physics
Reposted by Alessandro Zunino
May 20, 2025 at 6:45 PM
Reposted by Alessandro Zunino
🚨 Join us in shaping the future of #SmartMicroscopy! We're hiring PhD & fellowship positions at the intersection of #photonics, electronics & #AI.
Check out our works in this field
doi.org/10.1038/s414...
www.nature.com/articles/s41...
doi.org/10.1101/2024...
Please share!
#SPADarray #Microscopy
Check out our works in this field
doi.org/10.1038/s414...
www.nature.com/articles/s41...
doi.org/10.1101/2024...
Please share!
#SPADarray #Microscopy
April 23, 2025 at 9:12 AM
🚨 Join us in shaping the future of #SmartMicroscopy! We're hiring PhD & fellowship positions at the intersection of #photonics, electronics & #AI.
Check out our works in this field
doi.org/10.1038/s414...
www.nature.com/articles/s41...
doi.org/10.1101/2024...
Please share!
#SPADarray #Microscopy
Check out our works in this field
doi.org/10.1038/s414...
www.nature.com/articles/s41...
doi.org/10.1101/2024...
Please share!
#SPADarray #Microscopy
Reposted by Alessandro Zunino
Microscope objectives can be confusing, and detailed specs (or even basic properties) are often hard to find. Here's an introductory resource to help new builders and instrument designers with the basics. It's a work in progress so feedback is appreciated:
amsikking.github.io/microscope_o...
amsikking.github.io/microscope_o...
February 25, 2025 at 5:06 PM
Microscope objectives can be confusing, and detailed specs (or even basic properties) are often hard to find. Here's an introductory resource to help new builders and instrument designers with the basics. It's a work in progress so feedback is appreciated:
amsikking.github.io/microscope_o...
amsikking.github.io/microscope_o...