This work was made possible thanks to my amazing colleagues at the @vicidominilab.bsky.social in @iitalk.bsky.social . It is part of the #BrightEyes_ERC project funded by @erc.europa.eu. If you are curious about what else is cooking in the pot, check out our website:

vicidominilab.github.io

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The benefits of s²ISM extend to any other additional dimension contained in the ISM dataset, such as time and spectrum. We demonstrate superior fluorescence lifetime imaging, enabled by the single-photon timing capabilities of the #SPADarray and strengthened by our approach.

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The concept of s²ISM is general and versatile. It can be applied to any laser scanning technique equipped with a detector array. We demonstrate the feasibility of s²ISM with live-cell imaging, multi-color, and two-photon excitation fluorescence microscopy.

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The ISM dataset is inherently redundant and contains enough information to enable up-sampling of the reconstructed images. s²ISM exploits this property to relax Nyquist’s criterion by a factor of two and enable both optical and digital super-resolution.

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The s²ISM concept is simple: light spreads differently on the detector according to the emitter's axial position. With rigorous physical modelling of ISM image formation, we leverage such unique information to reconstruct only the in-focus fraction of the collected fluorescence photons.

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The benefits of s²ISM extend to any other additional dimension contained in the ISM dataset, such as time and spectrum. We demonstrate superior fluorescence lifetime imaging, enabled by the single-photon timing capabilities of the #SPADarray and strengthened by our approach.

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The concept of s²ISM is general and versatile. It can be applied to any laser scanning technique equipped with a detector array. We demonstrate the feasibility of s²ISM with live-cell imaging, multi-color, and two-photon excitation fluorescence microscopy.

🧵4/7

The ISM dataset is inherently redundant and contains enough information to enable up-sampling of the reconstructed images. s²ISM exploits this property to relax Nyquist’s criterion by a factor of two and enable both optical and digital super-resolution.

🧵3/7

The s²ISM concept is simple: light spreads differently on the detector according to the emitter's axial position. With rigorous physical modelling of ISM image formation, we leverage such unique information to reconstruct only the in-focus fraction of the collected fluorescence photons.

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Our last paper on s²ISM (super-resolution sectioning #ImageScanningMicroscopy) just got published on Nature Photonics @natphoton.nature.com‬! Discover how structured detection in laser scanning microscopes enhances both resolution and optical sectioning:

www.nature.com/articles/s41...

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