Bryan Bryson
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thebrysonlab.bsky.social
Bryan Bryson
@thebrysonlab.bsky.social
Tuberculosis, systems immunology, innate immunity, vaccines, computational biology
Excited to share our newest manuscript on antigen discovery and vaccine design for tuberculosis lead by Owen Leddy (everyone hiring new faculty in a few years, remember this name!)

www.science.org/doi/10.1126/... #TBsky
Immunopeptidomics can inform the design of mRNA vaccines for the delivery of Mycobacterium tuberculosis MHC class II antigens
MHC class II immunopeptidomics in human phagocytes infected with Mycobacterium tuberculosis enables vaccine immunogen design and optimization.
www.science.org
November 5, 2025 at 9:57 PM
Congrats to the Carpenter Lab on this exciting study!

I might now sound like a broken record, but check the abstract!!!

Let’s go type 7 secretion system substrates! #tuberculosis #tbsky
September 25, 2025 at 11:50 AM
Happy to see more data pointing to an important role for type 7 secretion substrates in TB protective immunity
September 17, 2025 at 2:29 PM
Reposted by Bryan Bryson
New video summary 🎥 from Leddy et al. @thebrysonlab.bsky.social (rupress.org/jem/article/... ) on a new workflow called PathMHC that helps find pathogen-derived antigens presented on MHCs by infected cells that could be targets for T cell priming vaccines:
Targeting infection-specific peptides in immunopeptidomics studies for vaccine target discovery
Original paper: https://rupress.org/jem/article/222/10/e20250444/278135/Targeting-infection-specific-peptides-in Video summary by Owen Leddy (Massachusetts Institute of Technology) Description: One…
youtu.be
August 18, 2025 at 6:15 PM
Newest paper from the lab out today (doi.org/10.1084/jem....)! Here, we describe PathMHC, a novel MS-based approach to identify rare pathogen-derived peptides on MHC-I and MHC-II. We apply this to a range of pathogens Mtb, SARS-CoV-2, and Listeria.
Targeting infection-specific peptides in immunopeptidomics studies for vaccine target discovery
MS-based immunopeptidomics can facilitate vaccine antigen discovery, but self-MHC peptides greatly outnumber pathogen-derived MHC peptides, presenting a ch
doi.org
July 21, 2025 at 2:19 PM
Anyone have experience with IV mRNA-LNP vaccination in animals? I am specifically interested in LNP formulations and what the preferred formulation for IV is.
July 9, 2025 at 2:16 PM
Pictured: glass of champagne I treated myself to at DCA upon learning the lab has been tenured!

Grateful to my lab family, my extended science family, and my family for the amazing support and love offered on the journey so far.

Much more work left to do but taking this moment to say thank you!
May 5, 2025 at 8:37 PM
Does anyone know of a CRO that produces MVA-based vaccines?
April 5, 2025 at 5:54 PM
Do any of my #tbsky friends have interests in T cell responses in people with evidence of prior Mtb infection?

If so, would you have any interest in looking at T cell responses to Mtb-derived peptides that we have detected by mass spectrometry? LMK 🙏🏽
March 28, 2025 at 6:04 PM
Reposted by Bryan Bryson
First post here! To inaugurate this account, I'd like to share my recent work, now published in JACS.

Intercepting a Mycobacterial Biosynthetic Pathway with Covalent Labeling | Journal of the American Chemical Society pubs.acs.org/doi/10.1021/...
Intercepting a Mycobacterial Biosynthetic Pathway with Covalent Labeling
The mycobacterial cell envelope plays both infectious and protective roles. Understanding its structure is crucial for unlocking the molecular basis underlying these functions. Studying glycans, the p...
pubs.acs.org
March 27, 2025 at 6:16 PM
Does anyone have a favorite protocol for in vitro priming of human T cells from PBMCs? #immunosky
January 3, 2025 at 8:09 PM
So glad to see this out! I learned a lot in this project about how to think creatively about customizing our approach to using language models for immune receptors
A bit over 3 years in the making, with first preprint out ~2 years ago, AbMAP has been a labor of love. Joint work with Chiho Im, an amazing MIT undergrad and now a fantastic Stanford grad student, and with Bonnie Berger and @thebrysonlab.bsky.social and @samsl.io and Lena Erlach and ...
January 2, 2025 at 5:47 PM
Immunopeptidomics (impep) friends, have you ever done impep to ID peptides after soluble protein delivery to phagocytes to look at peptides generated by XP?
December 12, 2024 at 1:28 PM
Before I write my own analysis script, does anyone in #tbsky have a tool for pathway analysis for Mtb genes?
December 7, 2024 at 6:29 PM
A lovely day to celebrate the amazing people I work with every day
December 6, 2024 at 1:06 AM
What is your favorite cytosolic pathogen that (a) infects macrophages and (b) is genetically tractable?

Looking for someone who’d like to team up on a small but impactful project (we would do the heavy lifting!)
November 27, 2024 at 7:54 PM
My whole scientific identity over the last 96 hours has been species differences in immunity. Would love to connect with others on this topic!
November 22, 2024 at 8:42 PM
Anyone on here love cathepsins or other lysosomal proteases? Would love to pick your brain for 15 minutes!
November 17, 2024 at 11:34 AM
Anyone want to discuss T cell vaccines? I’ve been in a rabbit hole lately and would love to discuss with others!
November 12, 2024 at 9:42 AM
Reposted by Bryan Bryson
I made a Starter Pack for the Myco & TB community.
Hope to find more good friends here on Bluesky to keep in touch about mycobacterial infections. Please join and repost!

go.bsky.app/3nwpcVV
November 11, 2024 at 7:54 AM
Looking forward to spending more time on here!

Tuberculosis, macrophages, antigen presentation, and systems biology folks — say hey!
November 10, 2024 at 3:28 PM
#Immunology friends, what are your favorite fix/perm conditions for imaging to access antigens in the phagosomal lumen?
November 3, 2023 at 12:40 AM
Looking forward to building community on here!

Just saying hello for now!

Bioengineer at MIT and the Ragon Institute with an interest in tuberculosis, innate immunity, and computational biology!

#HiSciSky #HiImmunoSky
September 30, 2023 at 10:53 PM