Sarah Bigot
@sbigot.bsky.social
Director of research CNRS at MMSB (C. Lesterlin/tacc team) interested in cellular and molecular mechanism of horizontal gene transfer
https://mmsb.cnrs.fr/equipe/transfert-d-adn-entre-cellules-bacteriennes/
https://mmsb.cnrs.fr/equipe/transfert-d-adn-entre-cellules-bacteriennes/
Reposted by Sarah Bigot
New paper with my (amazing) friend and mentor @jrpenades.bsky.social
Really looking forward to see what plasmid aficionados think of this one!!
With @asantoslopez.bsky.social @wfigueroac3.bsky.social Akshay Sabins and others
www.cell.com/cell-reports...
Really looking forward to see what plasmid aficionados think of this one!!
With @asantoslopez.bsky.social @wfigueroac3.bsky.social Akshay Sabins and others
www.cell.com/cell-reports...
Non-conjugative plasmids limit their mobility to persist in nature
Sabnis et al. explain why non-conjugative plasmids move at a low rate in nature. While
increased mobility can easily evolve by incorporating phage DNA into plasmids, this
is disadvantageous because it...
www.cell.com
October 22, 2025 at 1:12 PM
New paper with my (amazing) friend and mentor @jrpenades.bsky.social
Really looking forward to see what plasmid aficionados think of this one!!
With @asantoslopez.bsky.social @wfigueroac3.bsky.social Akshay Sabins and others
www.cell.com/cell-reports...
Really looking forward to see what plasmid aficionados think of this one!!
With @asantoslopez.bsky.social @wfigueroac3.bsky.social Akshay Sabins and others
www.cell.com/cell-reports...
Reposted by Sarah Bigot
New pre-print: Plasmid dependent phage effectively eliminate AMR bacteria and block plasmid transmission in the chicken gut microbiome
Fun collaboration with Tao He lab (JAAS) and @brockhurstlab.bsky.social lab (Manchester)
#phagesky#microsky
www.biorxiv.org/content/10.1...
Fun collaboration with Tao He lab (JAAS) and @brockhurstlab.bsky.social lab (Manchester)
#phagesky#microsky
www.biorxiv.org/content/10.1...
Plasmid dependent phage eliminate pathogenic bacteria and antibiotic resistance plasmids from the chicken gut microbiome
Conjugative plasmids are a key reservoir of antimicrobial resistance (AMR) in commensal and pathogenic bacteria within the gut microbiome. Plasmid-dependent phage (PDPs) are a promising therapeutic op...
www.biorxiv.org
October 27, 2025 at 8:19 AM
New pre-print: Plasmid dependent phage effectively eliminate AMR bacteria and block plasmid transmission in the chicken gut microbiome
Fun collaboration with Tao He lab (JAAS) and @brockhurstlab.bsky.social lab (Manchester)
#phagesky#microsky
www.biorxiv.org/content/10.1...
Fun collaboration with Tao He lab (JAAS) and @brockhurstlab.bsky.social lab (Manchester)
#phagesky#microsky
www.biorxiv.org/content/10.1...
Reposted by Sarah Bigot
JOB OFFER #PhageSky
We have a postdoc position opening in my group to investigate phage-MGE interactions !
We're based in the very nice city of Lyon, France @mmsb-lyon.bsky.social
Contact me for more info !
>> Apply on the CNRS webpage emploi.cnrs.fr/Offres/CDD/U...
We have a postdoc position opening in my group to investigate phage-MGE interactions !
We're based in the very nice city of Lyon, France @mmsb-lyon.bsky.social
Contact me for more info !
>> Apply on the CNRS webpage emploi.cnrs.fr/Offres/CDD/U...
Portail Emploi CNRS - Offre d'emploi - Postdoctoral researcher Microbiology (M/F)
emploi.cnrs.fr
October 22, 2025 at 9:35 AM
JOB OFFER #PhageSky
We have a postdoc position opening in my group to investigate phage-MGE interactions !
We're based in the very nice city of Lyon, France @mmsb-lyon.bsky.social
Contact me for more info !
>> Apply on the CNRS webpage emploi.cnrs.fr/Offres/CDD/U...
We have a postdoc position opening in my group to investigate phage-MGE interactions !
We're based in the very nice city of Lyon, France @mmsb-lyon.bsky.social
Contact me for more info !
>> Apply on the CNRS webpage emploi.cnrs.fr/Offres/CDD/U...
Reposted by Sarah Bigot
Check out the first preprint from our lab describing a direct molecular cross-talk between (p)ppGpp and c-di-GMP nucleotide messengers! Great work from first-author Corentin Jaboulay who was a postdoc in our lab, and great collaborations! @mmsb-lyon.bsky.social @cnrsbiologie.bsky.social
Cross-regulation of (p)ppGpp and c-di-GMP pathways controls a cell-cycle transition https://www.biorxiv.org/content/10.1101/2025.08.22.671821v1
August 24, 2025 at 7:48 AM
Check out the first preprint from our lab describing a direct molecular cross-talk between (p)ppGpp and c-di-GMP nucleotide messengers! Great work from first-author Corentin Jaboulay who was a postdoc in our lab, and great collaborations! @mmsb-lyon.bsky.social @cnrsbiologie.bsky.social
Reposted by Sarah Bigot
Open position !!
Either at the Engineer or post-doctoral level, to work on filamentous phages. Collaboration between Laetitia Houot's group and Thierry Doan @thicoz.bsky.social.
To apply at the E level:
emploi.cnrs.fr/Offres/CDD/U...
To apply at the post-doc level:
emploi.cnrs.fr/Offres/CDD/U...
Either at the Engineer or post-doctoral level, to work on filamentous phages. Collaboration between Laetitia Houot's group and Thierry Doan @thicoz.bsky.social.
To apply at the E level:
emploi.cnrs.fr/Offres/CDD/U...
To apply at the post-doc level:
emploi.cnrs.fr/Offres/CDD/U...
September 28, 2025 at 9:24 AM
Open position !!
Either at the Engineer or post-doctoral level, to work on filamentous phages. Collaboration between Laetitia Houot's group and Thierry Doan @thicoz.bsky.social.
To apply at the E level:
emploi.cnrs.fr/Offres/CDD/U...
To apply at the post-doc level:
emploi.cnrs.fr/Offres/CDD/U...
Either at the Engineer or post-doctoral level, to work on filamentous phages. Collaboration between Laetitia Houot's group and Thierry Doan @thicoz.bsky.social.
To apply at the E level:
emploi.cnrs.fr/Offres/CDD/U...
To apply at the post-doc level:
emploi.cnrs.fr/Offres/CDD/U...
Reposted by Sarah Bigot
Happy to share our work on a T6SS effector with ADP ribose cyclase activity, published in JBC @asbmbjournals.bsky.social
Great work of Julius Martinkus @jmartinkus.bsky.social and (always) great collaboration with @dukasju.bsky.social and @laurentterradot.bsky.social
www.jbc.org/article/S002...
Great work of Julius Martinkus @jmartinkus.bsky.social and (always) great collaboration with @dukasju.bsky.social and @laurentterradot.bsky.social
www.jbc.org/article/S002...
Widespread deployment of the human CD38 ADP-ribosyl cyclase fold in antibacterial and anti-eukaryotic polymorphic toxins
Bacterial polymorphic toxins are modular weapons that mediate inter-microbial competition
and host interactions by delivering diverse cytotoxic domains through specialized
secretion systems. Here, we ...
www.jbc.org
September 28, 2025 at 9:20 AM
Happy to share our work on a T6SS effector with ADP ribose cyclase activity, published in JBC @asbmbjournals.bsky.social
Great work of Julius Martinkus @jmartinkus.bsky.social and (always) great collaboration with @dukasju.bsky.social and @laurentterradot.bsky.social
www.jbc.org/article/S002...
Great work of Julius Martinkus @jmartinkus.bsky.social and (always) great collaboration with @dukasju.bsky.social and @laurentterradot.bsky.social
www.jbc.org/article/S002...
Reposted by Sarah Bigot
Delighted to see our paper studying the evolution of plasmids over the last 100 years, now out! Years of work by Adrian Cazares, also Nick Thomson @sangerinstitute.bsky.social - this version much improved over the preprint. Final version should be open access, apols.
Thread 1/n
Thread 1/n
September 25, 2025 at 9:29 PM
Delighted to see our paper studying the evolution of plasmids over the last 100 years, now out! Years of work by Adrian Cazares, also Nick Thomson @sangerinstitute.bsky.social - this version much improved over the preprint. Final version should be open access, apols.
Thread 1/n
Thread 1/n
Reposted by Sarah Bigot
📝New preprint! #MicroSky
🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.
🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.
www.biorxiv.org/content/10.1...
🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.
🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.
www.biorxiv.org/content/10.1...
Dynamics and interplay of gene expression and chromosome organization across a predatory lifecycle
The obligate predatory bacterium Bdellovibrio bacteriovorus alternates between a motile attack phase and a growth phase inside another bacterium, during which it undergoes multiple rounds of DNA repli...
www.biorxiv.org
September 25, 2025 at 7:12 AM
📝New preprint! #MicroSky
🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.
🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.
www.biorxiv.org/content/10.1...
🕰️We chart the full, synchronized lifecycle of a predatory bacterium using Hi-C, RNA-seq, ChIP-seq & microscopy.
🧬We uncover dramatic shifts in nucleoid architecture & transcription, tightly coordinated with predation.
www.biorxiv.org/content/10.1...
Reposted by Sarah Bigot
Exclusion systems, far from slowing down plasmid spread, are in fact essential to preserve host cell viability and thereby ensure the successful dissemination of conjugative plasmids and antibiotic resistance genes.
@nfrk92.bsky.social
@narjournal.bsky.social
academic.oup.com/nar/article/...
@nfrk92.bsky.social
@narjournal.bsky.social
academic.oup.com/nar/article/...
Exclusion systems preserve host cell homeostasis and fitness, ensuring successful dissemination of conjugative plasmids and associated resistance genes
Abstract. Plasmid conjugation is a major driver of antibiotic resistance dissemination in bacteria. In addition to genes required for transfer and maintena
academic.oup.com
September 17, 2025 at 10:17 AM
Exclusion systems, far from slowing down plasmid spread, are in fact essential to preserve host cell viability and thereby ensure the successful dissemination of conjugative plasmids and antibiotic resistance genes.
@nfrk92.bsky.social
@narjournal.bsky.social
academic.oup.com/nar/article/...
@nfrk92.bsky.social
@narjournal.bsky.social
academic.oup.com/nar/article/...
Reposted by Sarah Bigot
🚨New paper out! #MicroSky
Studying obligate predators like Bdellovibrio bacteriovorus is tricky—essential genes for predation are also essential for survival.
We expanded its genetic toolbox:
🧬promoters to fine-tune expression
🧬IPTG-inducible system
🧬CRISPRi for rapid knockdown
bit.ly/46GUn2c
1/4
Studying obligate predators like Bdellovibrio bacteriovorus is tricky—essential genes for predation are also essential for survival.
We expanded its genetic toolbox:
🧬promoters to fine-tune expression
🧬IPTG-inducible system
🧬CRISPRi for rapid knockdown
bit.ly/46GUn2c
1/4
Expanding the genetic toolbox of the obligate predatory bacterium Bdellovibrio bacteriovorus with inducible gene expression and CRISPR interference
Inducible gene expression and CRISPR interference-mediated depletion in the predatory bacterium Bdellovibrio bacteriovorus.
academic.oup.com
September 17, 2025 at 7:27 AM
🚨New paper out! #MicroSky
Studying obligate predators like Bdellovibrio bacteriovorus is tricky—essential genes for predation are also essential for survival.
We expanded its genetic toolbox:
🧬promoters to fine-tune expression
🧬IPTG-inducible system
🧬CRISPRi for rapid knockdown
bit.ly/46GUn2c
1/4
Studying obligate predators like Bdellovibrio bacteriovorus is tricky—essential genes for predation are also essential for survival.
We expanded its genetic toolbox:
🧬promoters to fine-tune expression
🧬IPTG-inducible system
🧬CRISPRi for rapid knockdown
bit.ly/46GUn2c
1/4
Reposted by Sarah Bigot
I wrote a Preview in @cp-cellhostmicrobe.bsky.social !
A new study suggests that #curli may protect prey from bacterial predators like #Bdellovibrio.
Really looking forward to seeing this model confirmed with live-cell imaging and specific reporters!
www.cell.com/cell-host-mi...
A new study suggests that #curli may protect prey from bacterial predators like #Bdellovibrio.
Really looking forward to seeing this model confirmed with live-cell imaging and specific reporters!
www.cell.com/cell-host-mi...
When predators meet the matrix: Natural resistance via amyloid fibers
How do bacteria protect themselves against predators? In a recent issue of Nature,
Ledvina et al. reveal curli fibers as a genetically encoded defense against Bdellovibrio
predation in wild E. coli st...
www.cell.com
September 11, 2025 at 9:18 AM
I wrote a Preview in @cp-cellhostmicrobe.bsky.social !
A new study suggests that #curli may protect prey from bacterial predators like #Bdellovibrio.
Really looking forward to seeing this model confirmed with live-cell imaging and specific reporters!
www.cell.com/cell-host-mi...
A new study suggests that #curli may protect prey from bacterial predators like #Bdellovibrio.
Really looking forward to seeing this model confirmed with live-cell imaging and specific reporters!
www.cell.com/cell-host-mi...
Reposted by Sarah Bigot
Check out @julielebris.bsky.social’s thread on our latest manuscript describing phenotypic heterogeneity in capsule production in Klebsiella & Acinetobacter @klebclub.bsky.social
This work started when I was still in @pasteur.fr & got finished in @cbitoulouse.bsky.social
#microsky #phagesky
This work started when I was still in @pasteur.fr & got finished in @cbitoulouse.bsky.social
#microsky #phagesky
Delighted to share our recently published work!
Ever wondered how Klebsiella (and others) deals with capsule production’s costs ?
The paper: journals.asm.org/doi/10.1128/...
Thread👇
Ever wondered how Klebsiella (and others) deals with capsule production’s costs ?
The paper: journals.asm.org/doi/10.1128/...
Thread👇
Phenotypic heterogeneity of capsule production across opportunistic pathogens | mBio
The polysaccharidic capsule is present in ~50% of species across the bacterial phylogeny, including all ESKAPE microorganisms, the six most significant multidrug-resistant (MDR) nosocomial pathogens. It is also an important virulence factor and a major ...
journals.asm.org
September 9, 2025 at 5:11 AM
Check out @julielebris.bsky.social’s thread on our latest manuscript describing phenotypic heterogeneity in capsule production in Klebsiella & Acinetobacter @klebclub.bsky.social
This work started when I was still in @pasteur.fr & got finished in @cbitoulouse.bsky.social
#microsky #phagesky
This work started when I was still in @pasteur.fr & got finished in @cbitoulouse.bsky.social
#microsky #phagesky
Reposted by Sarah Bigot
So excited our antibiotic potentiation story is out 🤩 Led by the extraordinary @manonlang.bsky.social with @fox-science.bsky.social & @amazeld.bsky.social +amazing collaborators @immunobladder.bsky.social @imaneelmeouche.bsky.social 🦠 We believe it can make a difference in #AMR infections!
Uridine as a potentiator of aminoglycosides through activation of carbohydrate transporters
Uridine boosts aminoglycoside treatment efficiency against antibiotic-susceptible as well as antibiotic-resistant E. coli strains.
www.science.org
September 6, 2025 at 9:35 AM
So excited our antibiotic potentiation story is out 🤩 Led by the extraordinary @manonlang.bsky.social with @fox-science.bsky.social & @amazeld.bsky.social +amazing collaborators @immunobladder.bsky.social @imaneelmeouche.bsky.social 🦠 We believe it can make a difference in #AMR infections!
Reposted by Sarah Bigot
Happy to see our paper out!
Functional assessment of anti-phage defence systems in their native clinical E. coli host
royalsocietypublishing.org/doi/10.1098/...
Thread in previous post 👇
Functional assessment of anti-phage defence systems in their native clinical E. coli host
royalsocietypublishing.org/doi/10.1098/...
Thread in previous post 👇
September 5, 2025 at 10:44 AM
Happy to see our paper out!
Functional assessment of anti-phage defence systems in their native clinical E. coli host
royalsocietypublishing.org/doi/10.1098/...
Thread in previous post 👇
Functional assessment of anti-phage defence systems in their native clinical E. coli host
royalsocietypublishing.org/doi/10.1098/...
Thread in previous post 👇
Reposted by Sarah Bigot
#ResultatScientifique🔎| Des scientifiques ont révélé comment le plasmide pOXA-48 rend les bactéries ultra-résistantes aux antibiotiques 🦠
🤝 @cnrs.fr @mmsb-lyon.bsky.social @cnrs-rhoneauvergne.bsky.social
✍️ @sbigot.bsky.social
📕 @natcomms.nature.com | buff.ly/u3Cy4Db
🤝 @cnrs.fr @mmsb-lyon.bsky.social @cnrs-rhoneauvergne.bsky.social
✍️ @sbigot.bsky.social
📕 @natcomms.nature.com | buff.ly/u3Cy4Db
September 4, 2025 at 8:30 AM
#ResultatScientifique🔎| Des scientifiques ont révélé comment le plasmide pOXA-48 rend les bactéries ultra-résistantes aux antibiotiques 🦠
🤝 @cnrs.fr @mmsb-lyon.bsky.social @cnrs-rhoneauvergne.bsky.social
✍️ @sbigot.bsky.social
📕 @natcomms.nature.com | buff.ly/u3Cy4Db
🤝 @cnrs.fr @mmsb-lyon.bsky.social @cnrs-rhoneauvergne.bsky.social
✍️ @sbigot.bsky.social
📕 @natcomms.nature.com | buff.ly/u3Cy4Db
Reposted by Sarah Bigot
At last ! A tool specifically for phage-plasmid hunters. Check tyPPing by @karinailchenko.bsky.social and @eugenpfeifer.bsky.social
🚨 New preprint! 🧬
𝐏𝐡𝐚𝐠𝐞-𝐩𝐥𝐚𝐬𝐦𝐢𝐝𝐬 (𝐏-𝐏𝐬) are fascinating elements: both 𝐭𝐞𝐦𝐩𝐞𝐫𝐚𝐭𝐞 𝐩𝐡𝐚𝐠𝐞𝐬 and 𝐩𝐥𝐚𝐬𝐦𝐢𝐝𝐬 ➡️ tricky to detect.
We present 𝐭𝐲𝐏𝐏𝐢𝐧𝐠 — the first 𝐢𝐝𝐞𝐧𝐭𝐢𝐟𝐢𝐜𝐚𝐭𝐢𝐨𝐧 𝐚𝐧𝐝 𝐜𝐥𝐚𝐬𝐬𝐢𝐟𝐢𝐜𝐚𝐭𝐢𝐨𝐧 𝐭𝐨𝐨𝐥 🛠️designed specifically for P-Ps:
✅ Accurate
✅ Sensitive
✅ Easy to use
📖 www.biorxiv.org/content/10.1...
𝐏𝐡𝐚𝐠𝐞-𝐩𝐥𝐚𝐬𝐦𝐢𝐝𝐬 (𝐏-𝐏𝐬) are fascinating elements: both 𝐭𝐞𝐦𝐩𝐞𝐫𝐚𝐭𝐞 𝐩𝐡𝐚𝐠𝐞𝐬 and 𝐩𝐥𝐚𝐬𝐦𝐢𝐝𝐬 ➡️ tricky to detect.
We present 𝐭𝐲𝐏𝐏𝐢𝐧𝐠 — the first 𝐢𝐝𝐞𝐧𝐭𝐢𝐟𝐢𝐜𝐚𝐭𝐢𝐨𝐧 𝐚𝐧𝐝 𝐜𝐥𝐚𝐬𝐬𝐢𝐟𝐢𝐜𝐚𝐭𝐢𝐨𝐧 𝐭𝐨𝐨𝐥 🛠️designed specifically for P-Ps:
✅ Accurate
✅ Sensitive
✅ Easy to use
📖 www.biorxiv.org/content/10.1...
Efficient detection and typing of phage-plasmids
Phage-plasmids are temperate phages that replicate as plasmids during lysogeny. Despite their high diversity, they carry genes similar to phages and plasmids. This leads to gene exchanges, and to the ...
www.biorxiv.org
September 3, 2025 at 2:00 PM
At last ! A tool specifically for phage-plasmid hunters. Check tyPPing by @karinailchenko.bsky.social and @eugenpfeifer.bsky.social
Reposted by Sarah Bigot
PhD opportunity, please share:
We are looking for a prospective PhD student to start in October 2026 who is excited about bacteria (Klebsiella pneumoniae), how they interact, and how they exchange DNA.
All details can be found on the funder website 👉 gw4biomed.ac.uk/developing-c...
We are looking for a prospective PhD student to start in October 2026 who is excited about bacteria (Klebsiella pneumoniae), how they interact, and how they exchange DNA.
All details can be found on the funder website 👉 gw4biomed.ac.uk/developing-c...
Developing CRISPR-Cas antimicrobials to tackle antibiotic resistance spread in Klebsiella pneumoniae - GW4 BioMed MRC DTP
Project Code MRCIIAR26Ex van Houte Project Type Wet lab Research Theme Infection, Immunity, Antimicrobial Resistance and Repair Project Summary Download Summary Antimicrobial resistance (AMR) poses a ...
gw4biomed.ac.uk
September 2, 2025 at 9:48 AM
PhD opportunity, please share:
We are looking for a prospective PhD student to start in October 2026 who is excited about bacteria (Klebsiella pneumoniae), how they interact, and how they exchange DNA.
All details can be found on the funder website 👉 gw4biomed.ac.uk/developing-c...
We are looking for a prospective PhD student to start in October 2026 who is excited about bacteria (Klebsiella pneumoniae), how they interact, and how they exchange DNA.
All details can be found on the funder website 👉 gw4biomed.ac.uk/developing-c...
Reposted by Sarah Bigot
Cool preprint by Mathieu Berge @polardlab.bsky.social showing that the Spr1630 toxin (RipA) toxin targets the replication sliding-clamp (DnaN) and that this induces competence in Streptococcus pneumoniae. www.biorxiv.org/content/10.1...
A toxin/antitoxin system targeting the replication sliding-clamp induces competence in Streptococcus pneumoniae.
Streptococcus pneumoniae is a pathogenic bacterium capable of entering a cellular differentiation state, called competence, which enables it to acquire new genetic functions by natural transformation,...
www.biorxiv.org
September 3, 2025 at 7:01 AM
Cool preprint by Mathieu Berge @polardlab.bsky.social showing that the Spr1630 toxin (RipA) toxin targets the replication sliding-clamp (DnaN) and that this induces competence in Streptococcus pneumoniae. www.biorxiv.org/content/10.1...
Reposted by Sarah Bigot
Have you ever wondered what increasing environmental stress will do to microbial communities?
In our new preprint, @martinadalbello.bsky.social, Jeff Gore and I studied the impact of salinity on microbial community composition and function. 🧵 (1/5)
www.biorxiv.org/content/10.1...
In our new preprint, @martinadalbello.bsky.social, Jeff Gore and I studied the impact of salinity on microbial community composition and function. 🧵 (1/5)
www.biorxiv.org/content/10.1...
Microbial communities demonstrate robustness in stressful environments due to predictable composition shifts
Environmental stress reduces species growth rates, but its impact on the function of microbial communities is less clear. Here, we experimentally demonstrate that increasing salinity stress shifts com...
www.biorxiv.org
August 25, 2025 at 2:33 PM
Have you ever wondered what increasing environmental stress will do to microbial communities?
In our new preprint, @martinadalbello.bsky.social, Jeff Gore and I studied the impact of salinity on microbial community composition and function. 🧵 (1/5)
www.biorxiv.org/content/10.1...
In our new preprint, @martinadalbello.bsky.social, Jeff Gore and I studied the impact of salinity on microbial community composition and function. 🧵 (1/5)
www.biorxiv.org/content/10.1...
Reposted by Sarah Bigot
🚨🚨New paper out in @natcomms.nature.com!!
Come for the first large-scale analysis of plasmid copy number across species,
stay for one of the most intriguing results of my lab: universal scaling laws in plasmid biology! 📈🧬
👉 www.nature.com/articles/s41...
Come for the first large-scale analysis of plasmid copy number across species,
stay for one of the most intriguing results of my lab: universal scaling laws in plasmid biology! 📈🧬
👉 www.nature.com/articles/s41...
Universal rules govern plasmid copy number - Nature Communications
Plasmids exhibit a broad range of sizes and copies per cell, and these two parameters appear to be negatively correlated. Here, Ramiro-Martínez et al. analyse the copy number of thousands of diverse b...
www.nature.com
July 2, 2025 at 11:08 AM
🚨🚨New paper out in @natcomms.nature.com!!
Come for the first large-scale analysis of plasmid copy number across species,
stay for one of the most intriguing results of my lab: universal scaling laws in plasmid biology! 📈🧬
👉 www.nature.com/articles/s41...
Come for the first large-scale analysis of plasmid copy number across species,
stay for one of the most intriguing results of my lab: universal scaling laws in plasmid biology! 📈🧬
👉 www.nature.com/articles/s41...
Reposted by Sarah Bigot
New paper out!
This work is finally published! 🥳🧬
Plasmids are associated with very variable fitness costs in their different bacterial hosts. But, what is the contribution of each of the plasmid-genes in these host-specific effects? Study led by
@jorgesastred.bsky.social, @sanmillan.bsky.social and myself! 1/14
Plasmids are associated with very variable fitness costs in their different bacterial hosts. But, what is the contribution of each of the plasmid-genes in these host-specific effects? Study led by
@jorgesastred.bsky.social, @sanmillan.bsky.social and myself! 1/14
Dissecting pOXA-48 fitness effects in clinical Enterobacterales using plasmid-wide CRISPRi screens
Nature Communications - This study investigates the effects of the carbapenem resistance plasmid pOXA-48 in clinical enterobacteria. Using CRISPRi screens, the authors revealed that the...
rdcu.be
August 20, 2025 at 3:00 PM
New paper out!
Our paper “Genetic determinants of pOXA-48 plasmid maintenance and propagation in Escherichia coli” is now online in @natcomms.nature.com ✨
👉 www.nature.com/articles/s41...
@cnrs-rhoneauvergne.bsky.social @mmsb-lyon.bsky.social
👉 www.nature.com/articles/s41...
@cnrs-rhoneauvergne.bsky.social @mmsb-lyon.bsky.social
Genetic determinants of pOXA-48 plasmid maintenance and propagation in Escherichia coli - Nature Communications
pOXA-48 plasmids have emerged as key vectors of carbapenem resistance within Enterobacteriaceae. In this study, the authors use a transposon sequencing (Tn-seq) approach to identify genetic determinan...
www.nature.com
August 20, 2025 at 3:19 PM
Our paper “Genetic determinants of pOXA-48 plasmid maintenance and propagation in Escherichia coli” is now online in @natcomms.nature.com ✨
👉 www.nature.com/articles/s41...
@cnrs-rhoneauvergne.bsky.social @mmsb-lyon.bsky.social
👉 www.nature.com/articles/s41...
@cnrs-rhoneauvergne.bsky.social @mmsb-lyon.bsky.social
Reposted by Sarah Bigot
Happy to finally share this! Read here about the best strategy the the persistence of low-copy plasmids. @mariosanter.bsky.social @iwangtoknow.bsky.social doi.org/10.1038/s414...
The combination of active partitioning and toxin-antitoxin systems is most advantageous for low-copy plasmid fitness - Nature Communications
Effe et al. evaluate how specific plasmid traits influence plasmid fitness; they provide mechanistic insight into the persistence of large conjugative plasmids, including those that disseminate antibi...
doi.org
August 3, 2025 at 10:46 AM
Happy to finally share this! Read here about the best strategy the the persistence of low-copy plasmids. @mariosanter.bsky.social @iwangtoknow.bsky.social doi.org/10.1038/s414...
Reposted by Sarah Bigot
Our latest paper explores the factors shaping plasmid-bacteria associations and highlights why studying plasmid-host interactions of clinical relevance is key for understanding the evolution and spread of AMR.
Find out more 👉 plasmidlab.es/publications/
#AntibioticResistance
Find out more 👉 plasmidlab.es/publications/
#AntibioticResistance
Publications - Plasmidlab
Take a detailed look at our work Discover our latest results Our story on bacterial evolution research Explore our publication’s archive Browse the list below or enter our PubMed’s repository to…
plasmidlab.es
July 21, 2025 at 8:48 AM
Our latest paper explores the factors shaping plasmid-bacteria associations and highlights why studying plasmid-host interactions of clinical relevance is key for understanding the evolution and spread of AMR.
Find out more 👉 plasmidlab.es/publications/
#AntibioticResistance
Find out more 👉 plasmidlab.es/publications/
#AntibioticResistance
Reposted by Sarah Bigot
We wrote 2 📕 chapters with tips & tricks to identify protein-protein interactions in bacteria 🦠
1) In vivo proximity labeling with miniTurbo link.springer.com/protocol/10....
2) An adapted polar recruitment assay feat. E. coli as a test tube link.springer.com/protocol/10.....
Enjoy #MicroSky 🧪
1) In vivo proximity labeling with miniTurbo link.springer.com/protocol/10....
2) An adapted polar recruitment assay feat. E. coli as a test tube link.springer.com/protocol/10.....
Enjoy #MicroSky 🧪
a man wearing sunglasses and a white t-shirt is holding something in his hands .
ALT: a man wearing sunglasses and a white t-shirt is holding something in his hands .
media.tenor.com
July 16, 2025 at 11:05 AM
We wrote 2 📕 chapters with tips & tricks to identify protein-protein interactions in bacteria 🦠
1) In vivo proximity labeling with miniTurbo link.springer.com/protocol/10....
2) An adapted polar recruitment assay feat. E. coli as a test tube link.springer.com/protocol/10.....
Enjoy #MicroSky 🧪
1) In vivo proximity labeling with miniTurbo link.springer.com/protocol/10....
2) An adapted polar recruitment assay feat. E. coli as a test tube link.springer.com/protocol/10.....
Enjoy #MicroSky 🧪