Ryosuke Kojima
@ryosukekojima.bsky.social
Associate Professor at the Graduate School of Medicine, the University of Tokyo. Chemical Biology & Synthetic Biology
Pinned
Ryosuke Kojima
@ryosukekojima.bsky.social
· Dec 12
Barcoding of small extracellular vesicles with CRISPR-gRNA enables comprehensive, subpopulation-specific analysis of their biogenesis and release regulators - Nature Communications
The release processes of small extracellular vesicles (sEVs) remain poorly understood despite their importance. Here, Kunitake et al. present a screening platform called CIBER screening that enables e...
www.nature.com
Introducing our recent “CIBER screening” paper describing the use of CRISPR-gRNA-barcoded extracellular vesicles (EVs) for high-throughput identification of EV release regulators @naturecomms.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Our paper on the antigen fluorogenic sensor is featured as a supplementary cover in this week’s @jacs.acspublications.org
! Beautiful artwork by our co-author, Ryo Tachibana! Read it here: pubs.acs.org/doi/full/10....
#MyACSCover
! Beautiful artwork by our co-author, Ryo Tachibana! Read it here: pubs.acs.org/doi/full/10....
#MyACSCover
August 28, 2025 at 4:54 AM
Our paper on the antigen fluorogenic sensor is featured as a supplementary cover in this week’s @jacs.acspublications.org
! Beautiful artwork by our co-author, Ryo Tachibana! Read it here: pubs.acs.org/doi/full/10....
#MyACSCover
! Beautiful artwork by our co-author, Ryo Tachibana! Read it here: pubs.acs.org/doi/full/10....
#MyACSCover
Reposted by Ryosuke Kojima
Thrilled our PANCS-binder tech is finally out in print!
www.nature.com/articles/s41...
This technology, 11-years in the making, has transformed how we do research in my lab, and was led by an amazing postdoc, Matt Styles, who is currently entering the academic job market - so look out for him! 1/n
www.nature.com/articles/s41...
This technology, 11-years in the making, has transformed how we do research in my lab, and was led by an amazing postdoc, Matt Styles, who is currently entering the academic job market - so look out for him! 1/n
August 6, 2025 at 5:54 PM
Thrilled our PANCS-binder tech is finally out in print!
www.nature.com/articles/s41...
This technology, 11-years in the making, has transformed how we do research in my lab, and was led by an amazing postdoc, Matt Styles, who is currently entering the academic job market - so look out for him! 1/n
www.nature.com/articles/s41...
This technology, 11-years in the making, has transformed how we do research in my lab, and was led by an amazing postdoc, Matt Styles, who is currently entering the academic job market - so look out for him! 1/n
Reposted by Ryosuke Kojima
Our latest paper is finally published in @natcomms.nature.com (doi.org/10.1038/s414...) !! We successfully constructed synthetic multinuclear metal complexes using only proteins.
August 1, 2025 at 6:16 AM
Our latest paper is finally published in @natcomms.nature.com (doi.org/10.1038/s414...) !! We successfully constructed synthetic multinuclear metal complexes using only proteins.
抗原に結合するだけで光る蛍光プローブの新規設計法に関する論文がJACSで公開になりました! 日本語プレスリリースはこちらです。@jacs.acspublications.org www.u-tokyo.ac.jp/content/4002...
July 24, 2025 at 1:45 AM
抗原に結合するだけで光る蛍光プローブの新規設計法に関する論文がJACSで公開になりました! 日本語プレスリリースはこちらです。@jacs.acspublications.org www.u-tokyo.ac.jp/content/4002...
Excited to share that our new paper is out in
@jacs.acspublications.org !
We introduce a new design principle for fluorogenic antigen probes, using an intramolecular reaction between a thiol-reactive fluorophore and a cysteine engineered into a DARPin.
pubs.acs.org/doi/10.1021/...
@jacs.acspublications.org !
We introduce a new design principle for fluorogenic antigen probes, using an intramolecular reaction between a thiol-reactive fluorophore and a cysteine engineered into a DARPin.
pubs.acs.org/doi/10.1021/...
Design of Antigen-Targeting Fluorogenic Probes Utilizing Intramolecular Addition Reaction of Protein-Dye Hybrids
Fluorogenic probes for antigens are useful for various purposes, including medical diagnostics and imaging, but achieving a rapid, large fluorescence increase is difficult. Here, we report a new class...
pubs.acs.org
July 24, 2025 at 12:31 AM
Excited to share that our new paper is out in
@jacs.acspublications.org !
We introduce a new design principle for fluorogenic antigen probes, using an intramolecular reaction between a thiol-reactive fluorophore and a cysteine engineered into a DARPin.
pubs.acs.org/doi/10.1021/...
@jacs.acspublications.org !
We introduce a new design principle for fluorogenic antigen probes, using an intramolecular reaction between a thiol-reactive fluorophore and a cysteine engineered into a DARPin.
pubs.acs.org/doi/10.1021/...
5/12(月)に第75回日本電気泳動シンポジウム@ Shimadzu Tokyo Innovation Plaza (川崎殿町)を企画しております。非常に魅力的なスピーカー陣＆会場ですので、ご興味のある方はぜひご参加ください (参加者把握のため、事前登録をお願いします)。参加費無料です！https://jes1950.jp/J_MEETING/75th_sympo/75th_sympo.html
April 23, 2025 at 12:02 AM
5/12(月)に第75回日本電気泳動シンポジウム@ Shimadzu Tokyo Innovation Plaza (川崎殿町)を企画しております。非常に魅力的なスピーカー陣＆会場ですので、ご興味のある方はぜひご参加ください (参加者把握のため、事前登録をお願いします)。参加費無料です！https://jes1950.jp/J_MEETING/75th_sympo/75th_sympo.html
New preprint! 当ラボ卒業生の王さんを中心として、生体直交性の高い蛍光プローブ・改変レポーター酵素ペアを開発し、低バックグラウンドのがんイメージングを実現しました。新しいレポーターシステムとして, ELISA, ADEPT他、多用途に使えると考えています！
New preprint! In a study led by Wang-san, we developed a novel biorthogonal fluorescence probe and an engineered reporter enzyme, enabling low-background cancer imaging. This powerful pair could also be valuable for applications such as ELISA and ADEPT.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Low-Background Cancer Imaging With a Bioorthogonal Fluorescence Probe and Engineered Reporter Enzyme Bearing a Targeting Moiety
Combinatorial use of an antibody-reporter enzyme conjugate and a fluorescence probe activated by the enzyme is a powerful strategy for fluorescence-guided cancer surgery. However, conventional probes ...
www.biorxiv.org
March 14, 2025 at 2:01 AM
New preprint! 当ラボ卒業生の王さんを中心として、生体直交性の高い蛍光プローブ・改変レポーター酵素ペアを開発し、低バックグラウンドのがんイメージングを実現しました。新しいレポーターシステムとして, ELISA, ADEPT他、多用途に使えると考えています！
New preprint! In a study led by Wang-san, we developed a novel biorthogonal fluorescence probe and an engineered reporter enzyme, enabling low-background cancer imaging. This powerful pair could also be valuable for applications such as ELISA and ADEPT.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Low-Background Cancer Imaging With a Bioorthogonal Fluorescence Probe and Engineered Reporter Enzyme Bearing a Targeting Moiety
Combinatorial use of an antibody-reporter enzyme conjugate and a fluorescence probe activated by the enzyme is a powerful strategy for fluorescence-guided cancer surgery. However, conventional probes ...
www.biorxiv.org
March 14, 2025 at 1:48 AM
New preprint! In a study led by Wang-san, we developed a novel biorthogonal fluorescence probe and an engineered reporter enzyme, enabling low-background cancer imaging. This powerful pair could also be valuable for applications such as ELISA and ADEPT.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
当グループ(@東大医)で博士研究員を募集しています。細胞外小胞/細胞間コミュニケーションの理解や操作を可能とする合成生物学研究に興味のある方はぜひご検討ください。もちろん学振PDの応募などの相談にものれます。ご興味があればお気軽にDMください！https://www.m.u-tokyo.ac.jp/news/jobs/20250121.pdf
January 21, 2025 at 1:11 AM
当グループ(@東大医)で博士研究員を募集しています。細胞外小胞/細胞間コミュニケーションの理解や操作を可能とする合成生物学研究に興味のある方はぜひご検討ください。もちろん学振PDの応募などの相談にものれます。ご興味があればお気軽にDMください！https://www.m.u-tokyo.ac.jp/news/jobs/20250121.pdf
Reposted by Ryosuke Kojima
2025
= 45²
= (20 + 25)²
= (1 + 2 + 3 +4 + 5 + 6 + 7 + 8 + 9)²
= 1³ + 2³ + 3³ +4³ + 5³ + 6³ + 7³ + 8³ + 9³
= 45²
= (20 + 25)²
= (1 + 2 + 3 +4 + 5 + 6 + 7 + 8 + 9)²
= 1³ + 2³ + 3³ +4³ + 5³ + 6³ + 7³ + 8³ + 9³
December 30, 2024 at 1:14 PM
2025
= 45²
= (20 + 25)²
= (1 + 2 + 3 +4 + 5 + 6 + 7 + 8 + 9)²
= 1³ + 2³ + 3³ +4³ + 5³ + 6³ + 7³ + 8³ + 9³
= 45²
= (20 + 25)²
= (1 + 2 + 3 +4 + 5 + 6 + 7 + 8 + 9)²
= 1³ + 2³ + 3³ +4³ + 5³ + 6³ + 7³ + 8³ + 9³
Reposted by Ryosuke Kojima
Are you interested in studying enhancer biology/3D genome organization in my lab at the University of Tokyo? This Biohub Network program would be a great opportunity for you! Feel free to reach out if you're interested!!
www.czbiohub.org/program-ssfc...
December 19, 2024 at 1:06 AM
Are you interested in studying enhancer biology/3D genome organization in my lab at the University of Tokyo? This Biohub Network program would be a great opportunity for you! Feel free to reach out if you're interested!!
www.czbiohub.org/program-ssfc...
Reposted by Ryosuke Kojima
📣 Our website got a fresh makeover! 🔍 Check out our updated lab website!
sites.google.com/view/y-okamo...
sites.google.com/view/y-okamo...
Designer Biomolecular Chemistry - English
Member
Staff
Assoc. Prof. (PI) : Yasunori Okamoto
Assist. Prof. : –
Administrative assistant : Ayaka Noda
Research assistant : Tomoko Etoh
Student
It's you. Apply now！
sites.google.com
December 13, 2024 at 3:39 AM
📣 Our website got a fresh makeover! 🔍 Check out our updated lab website!
sites.google.com/view/y-okamo...
sites.google.com/view/y-okamo...
新作preprint @chemrxiv.bsky.social を公開しました。チオール反応性蛍光団と抗原認識タンパク質をうまく組み合わせて、抗原に結合して初めて光る蛍光センサーを開発しました。洗浄なしでのがんイメージングなどに応用可能です。卒業生の中館さんが中心となって研究を進めてくれました! bsky.app/profile/ryos...
New preprint @chemrxiv.bsky.social ! In this work led by Nakadate-san, we have developed a new type of fluorogenic sensor for an antigen that enables wash-free cancer imaging. Check out our unique probe design using a thiol-reactive fluorophore and DARPin! chemrxiv.org/engage/chemr...
New class of fluorogenic probes for antigens based on DARPin-silicon pyronine conjugates
Fluorogenic probes for antigens are useful for various purposes, including medical diagnostics and imaging, but achieving a rapid, large fluorescence increase is difficult. Here, we report a new class...
chemrxiv.org
December 12, 2024 at 1:29 AM
新作preprint @chemrxiv.bsky.social を公開しました。チオール反応性蛍光団と抗原認識タンパク質をうまく組み合わせて、抗原に結合して初めて光る蛍光センサーを開発しました。洗浄なしでのがんイメージングなどに応用可能です。卒業生の中館さんが中心となって研究を進めてくれました! bsky.app/profile/ryos...
New preprint @chemrxiv.bsky.social ! In this work led by Nakadate-san, we have developed a new type of fluorogenic sensor for an antigen that enables wash-free cancer imaging. Check out our unique probe design using a thiol-reactive fluorophore and DARPin! chemrxiv.org/engage/chemr...
New class of fluorogenic probes for antigens based on DARPin-silicon pyronine conjugates
Fluorogenic probes for antigens are useful for various purposes, including medical diagnostics and imaging, but achieving a rapid, large fluorescence increase is difficult. Here, we report a new class...
chemrxiv.org
December 12, 2024 at 1:28 AM
New preprint @chemrxiv.bsky.social ! In this work led by Nakadate-san, we have developed a new type of fluorogenic sensor for an antigen that enables wash-free cancer imaging. Check out our unique probe design using a thiol-reactive fluorophore and DARPin! chemrxiv.org/engage/chemr...
Introducing our recent “CIBER screening” paper describing the use of CRISPR-gRNA-barcoded extracellular vesicles (EVs) for high-throughput identification of EV release regulators @naturecomms.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Barcoding of small extracellular vesicles with CRISPR-gRNA enables comprehensive, subpopulation-specific analysis of their biogenesis and release regulators - Nature Communications
The release processes of small extracellular vesicles (sEVs) remain poorly understood despite their importance. Here, Kunitake et al. present a screening platform called CIBER screening that enables e...
www.nature.com
December 12, 2024 at 1:27 AM
Introducing our recent “CIBER screening” paper describing the use of CRISPR-gRNA-barcoded extracellular vesicles (EVs) for high-throughput identification of EV release regulators @naturecomms.bsky.social
www.nature.com/articles/s41...
www.nature.com/articles/s41...