Richard Held
@rgsheld.bsky.social
Neuroscientist / synaptic physiologist interested in protein topography and cellular signal transduction. Ephys in the past, cryoET now, mouse genetics forever.
https://scholar.google.com/citations?hl=en&user=ESpBK1AAAAAJ
https://scholar.google.com/citations?hl=en&user=ESpBK1AAAAAJ
Pinned
Richard Held
@rgsheld.bsky.social
· Nov 13
Reintro: I'm a neuroscientist interested in how cells use molecular topography to control signal transduction. I use #CryoET and mouse genetic tools to map the topography of synaptic proteins and determine in-situ structures of scaffolding interactions that control said topography.
Will be at SfN for the first time since 2011 this year (bad neuroscientist 😬). Hopefully see some of you there irl.
November 11, 2025 at 5:12 PM
Will be at SfN for the first time since 2011 this year (bad neuroscientist 😬). Hopefully see some of you there irl.
Finally got to go through this carefully. An interesting paper and a tremendous amount of work.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
“Kiss-shrink-run” unifies mechanisms for synaptic vesicle exocytosis and hyperfast recycling
Synaptic vesicle (SV) exocytosis underpins neuronal communication, yet its nanoscale dynamics remain poorly understood owing to limitations in visualizing rapid events in situ. Here, we used optogenet...
www.science.org
November 7, 2025 at 9:50 PM
Finally got to go through this carefully. An interesting paper and a tremendous amount of work.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Gonna need somebody to explain daylight savings to my dogs
November 3, 2025 at 2:11 AM
Gonna need somebody to explain daylight savings to my dogs
I think about this tweet on a near-daily basis
October 9, 2025 at 4:11 PM
I think about this tweet on a near-daily basis
This is what made America great
October 8, 2025 at 4:41 PM
This is what made America great
Reposted by Richard Held
The cat is officially out of the bag!
The LMB is home to a unique community of outstanding connectomics scientists — I'm so excited about adding my own molecular and expansion microscopy expertise to the mix!
This is going to be the most exciting journey of my scientific career — more soon! 🌈🧠
The LMB is home to a unique community of outstanding connectomics scientists — I'm so excited about adding my own molecular and expansion microscopy expertise to the mix!
This is going to be the most exciting journey of my scientific career — more soon! 🌈🧠
LMB welcomes @sventruckenbrodt.bsky.social as a new Group Leader in our Neurobiology Division!
Sven will develop molecular brain mapping, harnessing expansion microscopy & molecular biology methods to better understand the neural basis of behaviour.
www2.mrc-lmb.cam.ac.uk/the-lmb-welc... #LMBNews
Sven will develop molecular brain mapping, harnessing expansion microscopy & molecular biology methods to better understand the neural basis of behaviour.
www2.mrc-lmb.cam.ac.uk/the-lmb-welc... #LMBNews
September 15, 2025 at 9:54 AM
The cat is officially out of the bag!
The LMB is home to a unique community of outstanding connectomics scientists — I'm so excited about adding my own molecular and expansion microscopy expertise to the mix!
This is going to be the most exciting journey of my scientific career — more soon! 🌈🧠
The LMB is home to a unique community of outstanding connectomics scientists — I'm so excited about adding my own molecular and expansion microscopy expertise to the mix!
This is going to be the most exciting journey of my scientific career — more soon! 🌈🧠
Heart rate data or RC voltage step?
August 8, 2025 at 3:14 PM
Heart rate data or RC voltage step?
Saw someone designing a cloning strategy using chatgpt and for some reason that's kinda my breaking point in this particular discussion. How much thinking really needs to be outsourced for the sake of efficiency and who does that really serve in the end?
July 17, 2025 at 4:18 PM
Saw someone designing a cloning strategy using chatgpt and for some reason that's kinda my breaking point in this particular discussion. How much thinking really needs to be outsourced for the sake of efficiency and who does that really serve in the end?
Reposted by Richard Held
I have lots of opinions—but for your sake, I’ve limited them to segmentation in cryo-ET for this article 😉
www.sciencedirect.com/science/arti...
#teamtomo
www.sciencedirect.com/science/arti...
#teamtomo
Segmenting cryo-electron tomography data: Extracting models from cellular landscapes
Cryo-electron tomography provides an unprecedented view of cellular architecture, yet extracting meaningful biological insights remains challenging. S…
www.sciencedirect.com
July 11, 2025 at 12:46 AM
I have lots of opinions—but for your sake, I’ve limited them to segmentation in cryo-ET for this article 😉
www.sciencedirect.com/science/arti...
#teamtomo
www.sciencedirect.com/science/arti...
#teamtomo
Reposted by Richard Held
"The money the government sends to Harvard is, in effect, not a subsidy to advance the university’s mission. It’s a payment for the role Harvard plays in advancing the research mission of the United States." www.nytimes.com/interactive/...
Here Is All the Science at Risk in Trump’s Clash With Harvard (Gift Article)
More than 900 research grants worth $2.6 billion are in jeopardy. So is the 80-year-old model of American science.
www.nytimes.com
June 23, 2025 at 6:48 PM
"The money the government sends to Harvard is, in effect, not a subsidy to advance the university’s mission. It’s a payment for the role Harvard plays in advancing the research mission of the United States." www.nytimes.com/interactive/...
Boston is the best city to run in. This is a take I'm willing to fight over.
June 21, 2025 at 4:07 PM
Boston is the best city to run in. This is a take I'm willing to fight over.
Having come up through US academia the rug pull of the last few months has been pretty extreme. Wondering how much longer I (& everyone else) get to do science sucks but with some dead-man's logic it also makes me really appreciate the years that I got to spend asking questions for their own sake.
June 20, 2025 at 11:37 PM
Having come up through US academia the rug pull of the last few months has been pretty extreme. Wondering how much longer I (& everyone else) get to do science sucks but with some dead-man's logic it also makes me really appreciate the years that I got to spend asking questions for their own sake.
Reposted by Richard Held
Proud to share our latest paper. doi.org/10.1016/j.cr...
Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue.
Structural biology in tissue is well and truly here!
@rosfrankinst.bsky.social
Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue.
Structural biology in tissue is well and truly here!
@rosfrankinst.bsky.social
June 17, 2025 at 10:59 AM
Proud to share our latest paper. doi.org/10.1016/j.cr...
Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue.
Structural biology in tissue is well and truly here!
@rosfrankinst.bsky.social
Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue.
Structural biology in tissue is well and truly here!
@rosfrankinst.bsky.social
Reposted by Richard Held
A call to stand up and show support for the Bethesda Declaration, the NIH, and for federally funded science in general.
The Bethesda Declaration
www.science.org
June 9, 2025 at 6:13 PM
A call to stand up and show support for the Bethesda Declaration, the NIH, and for federally funded science in general.
A formidable opponent in the fight against productivity.
June 2, 2025 at 7:25 PM
A formidable opponent in the fight against productivity.
Reposted by Richard Held
New method toward modeling cells and viruses! HMFF enables integrative membrane modeling and simulation guided by electron microscopy data. Created by Valentin Maurer and Marc Siggel @embl.org @cssbhamburg.bsky.social, in collaboration with @WPezeshkian lab (1/n) www.biorxiv.org/content/10.1...
May 30, 2025 at 8:36 PM
New method toward modeling cells and viruses! HMFF enables integrative membrane modeling and simulation guided by electron microscopy data. Created by Valentin Maurer and Marc Siggel @embl.org @cssbhamburg.bsky.social, in collaboration with @WPezeshkian lab (1/n) www.biorxiv.org/content/10.1...
Reposted by Richard Held
Yesterday, the NIH R35 “Outstanding Investigator” grant to fund scientists in my lab studying antibiotic resistance was terminated for reasons not related to the content of the science, or any actions taken by me or members of my lab
May 13, 2025 at 11:37 PM
Yesterday, the NIH R35 “Outstanding Investigator” grant to fund scientists in my lab studying antibiotic resistance was terminated for reasons not related to the content of the science, or any actions taken by me or members of my lab
Ok microtubule people, what is going on here? Cross section projection images, two different areas and surrounded by intact microtubules:
May 13, 2025 at 4:30 PM
Ok microtubule people, what is going on here? Cross section projection images, two different areas and surrounded by intact microtubules:
Reposted by Richard Held
My colleagues @phaips.vd.st and @florentwaltz.bsky.social have put together this wonderful website with step-by-step tutorials for in situ subtomogram averaging using different software packages 🤩
Check it out, especially if you are a newcomer to the field!
#CryoET #TeamTomo #ChlamyDataset
Check it out, especially if you are a newcomer to the field!
#CryoET #TeamTomo #ChlamyDataset
Hey #TeamTomo,
Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging?
That's why @florentwaltz.bsky.social and I made this website!
tomoguide.github.io
Follow the thread 1 /🧵
#CryoET #CryoEM 🔬🧪
Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging?
That's why @florentwaltz.bsky.social and I made this website!
tomoguide.github.io
Follow the thread 1 /🧵
#CryoET #CryoEM 🔬🧪
Welcome to TomoGuide
A step-by-step Cryo-ET guide
tomoguide.github.io
May 7, 2025 at 6:28 AM
My colleagues @phaips.vd.st and @florentwaltz.bsky.social have put together this wonderful website with step-by-step tutorials for in situ subtomogram averaging using different software packages 🤩
Check it out, especially if you are a newcomer to the field!
#CryoET #TeamTomo #ChlamyDataset
Check it out, especially if you are a newcomer to the field!
#CryoET #TeamTomo #ChlamyDataset
Reposted by Richard Held
We have updated our #MemBrain v2 preprint with a lot more details about the MemBrain-pick and MemBrain-stats modules, as well as some application examples!
Stay tuned for the upcoming thread by lead author @lorenzlamm.bsky.social! 🧠🧵
#CryoET #TeamTomo
www.biorxiv.org/content/10.1...
Stay tuned for the upcoming thread by lead author @lorenzlamm.bsky.social! 🧠🧵
#CryoET #TeamTomo
www.biorxiv.org/content/10.1...
April 23, 2025 at 8:47 AM
We have updated our #MemBrain v2 preprint with a lot more details about the MemBrain-pick and MemBrain-stats modules, as well as some application examples!
Stay tuned for the upcoming thread by lead author @lorenzlamm.bsky.social! 🧠🧵
#CryoET #TeamTomo
www.biorxiv.org/content/10.1...
Stay tuned for the upcoming thread by lead author @lorenzlamm.bsky.social! 🧠🧵
#CryoET #TeamTomo
www.biorxiv.org/content/10.1...
Reposted by Richard Held
Thrilled to share these experiments on shaping focused ion beams to explore different ways of milling. Here, we explore using different beam geometries to generate cellular thin sections/lamellae. The video shows elongating the beam on a charging spot burn www.biorxiv.org/content/10.1...
April 9, 2025 at 1:07 PM
Thrilled to share these experiments on shaping focused ion beams to explore different ways of milling. Here, we explore using different beam geometries to generate cellular thin sections/lamellae. The video shows elongating the beam on a charging spot burn www.biorxiv.org/content/10.1...
Reposted by Richard Held
Exploring shaped focused ion beams for lamella preparation https://www.biorxiv.org/content/10.1101/2025.04.01.646235v1
April 6, 2025 at 10:47 PM
Exploring shaped focused ion beams for lamella preparation https://www.biorxiv.org/content/10.1101/2025.04.01.646235v1
Could run this article for every flight he takes
March 28, 2025 at 6:48 PM
Could run this article for every flight he takes
Maybe Jeffrey Goldberg is in all of our signal chats and we just have to step back from our lives for a bit to really notice him there.
March 28, 2025 at 4:53 PM
Maybe Jeffrey Goldberg is in all of our signal chats and we just have to step back from our lives for a bit to really notice him there.