Cathy Spangler
@cathyspangler.bsky.social
Postdoc at OHSU studying synapses and receptors using cryo-electron microscopy // cryo-EM & cryo-ET // also <3 ⚽ 🎻 ⛰️ ⛷️ 🐶
Out today, we can now multiplex receptor mapping at synapses thanks to a dimeric gold nanoparticle label optimized by Hoyoung that's distinguishable from monomers!
Dimeric gold nanoparticles enable multiplexed labeling in cryoelectron tomography | PNAS www.pnas.org/doi/10.1073/...
Dimeric gold nanoparticles enable multiplexed labeling in cryoelectron tomography | PNAS www.pnas.org/doi/10.1073/...
PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
www.pnas.org
November 24, 2025 at 8:54 PM
Out today, we can now multiplex receptor mapping at synapses thanks to a dimeric gold nanoparticle label optimized by Hoyoung that's distinguishable from monomers!
Dimeric gold nanoparticles enable multiplexed labeling in cryoelectron tomography | PNAS www.pnas.org/doi/10.1073/...
Dimeric gold nanoparticles enable multiplexed labeling in cryoelectron tomography | PNAS www.pnas.org/doi/10.1073/...
Excited to share the final version of this work from the group, led by Chengli! Always fun when an unexpected complex appears in a cryo-EM dataset 😀
rdcu.be/eym9v
rdcu.be/eym9v
Gating and noelin clustering of native Ca2+-permeable AMPA receptors
Nature - Cryo-electron microscopy analyses of calcium-permeable AMPA receptors from rat brain show that they are composed primarily of GluA1 and GluA4 subunits, and biochemical studies show that...
rdcu.be
July 29, 2025 at 4:14 PM
Excited to share the final version of this work from the group, led by Chengli! Always fun when an unexpected complex appears in a cryo-EM dataset 😀
rdcu.be/eym9v
rdcu.be/eym9v
I've been lucky to be able to work with Natalie on producing several new monoclonal antibodies using these methods, with great success! Check out our group's new protocols paper on how to generate mAbs for various membrane proteins -
currentprotocols.onlinelibrary.wiley.com/doi/full/10....
currentprotocols.onlinelibrary.wiley.com/doi/full/10....
Generation of Conformation‐Specific Monoclonal Antibodies for Integral Membrane Proteins
Antibodies and their antigen-binding fragments, including fragment antigen-binding domains (Fabs) and single-chain variable fragments (scFvs), are extraordinary tools in all fields of biology, partic....
currentprotocols.onlinelibrary.wiley.com
May 27, 2025 at 8:57 PM
I've been lucky to be able to work with Natalie on producing several new monoclonal antibodies using these methods, with great success! Check out our group's new protocols paper on how to generate mAbs for various membrane proteins -
currentprotocols.onlinelibrary.wiley.com/doi/full/10....
currentprotocols.onlinelibrary.wiley.com/doi/full/10....
So glad to see this out! Congrats!
My most challenging postdoctoral project, where we show that histones but not nucleosomes are polyubiqutinated by the APC/C for a potential proteasomal degradation was just published in @naturecomms.bsky.social
🎉
www.nature.com/articles/s41...
Big shout-out to all co-authors!!!
🎉
www.nature.com/articles/s41...
Big shout-out to all co-authors!!!
APC/C-mediated ubiquitylation of extranucleosomal histone complexes lacking canonical degrons - Nature Communications
The APC/C ubiquitylates histones to regulate gene expression in pluripotent cells. Here, the authors pair cryo-EM and biochemical and biophysical assays to show that instead of modifying nucleosome-in...
www.nature.com
March 21, 2025 at 12:41 AM
So glad to see this out! Congrats!
Congrats Makayla!
Thrilled to share the pre-print of my dissertation research on the bile acid-sensitive ion channel (BASIC)! 🥳
The bile acid-sensitive ion channel is gated by Ca2+-dependent conformational changes in the transmembrane domain https://www.biorxiv.org/content/10.1101/2025.01.10.632481v1
January 13, 2025 at 10:43 PM
Congrats Makayla!
Gotta love finding soccer balls in tomograms ⚽ 😂
December 4, 2024 at 7:49 PM
Gotta love finding soccer balls in tomograms ⚽ 😂
Loving the holiday spirit at PNCC to start off December 🎄 + early Xmas gift getting to test out Tomo5!
December 3, 2024 at 1:55 AM
Loving the holiday spirit at PNCC to start off December 🎄 + early Xmas gift getting to test out Tomo5!
First Bluesky post!
Our recent eLife paper is now an official version of record. This was my first project using #Cryo-ET and I'm definitely hooked - it's so fun to look at snapshots of synapses within brain tissue and see individual proteins. Excited to share more soon!
doi.org/10.7554/eLif...
Our recent eLife paper is now an official version of record. This was my first project using #Cryo-ET and I'm definitely hooked - it's so fun to look at snapshots of synapses within brain tissue and see individual proteins. Excited to share more soon!
doi.org/10.7554/eLif...
Cryo-electron tomographic investigation of native hippocampal glutamatergic synapses
By harnessing antibody fragments conjugated to small gold nanoparticles together with high pressure freezing, FIB/SEM milling and cryo-electron tomography, AMPA receptors were identified from mouse br...
doi.org
November 24, 2024 at 5:57 PM
First Bluesky post!
Our recent eLife paper is now an official version of record. This was my first project using #Cryo-ET and I'm definitely hooked - it's so fun to look at snapshots of synapses within brain tissue and see individual proteins. Excited to share more soon!
doi.org/10.7554/eLif...
Our recent eLife paper is now an official version of record. This was my first project using #Cryo-ET and I'm definitely hooked - it's so fun to look at snapshots of synapses within brain tissue and see individual proteins. Excited to share more soon!
doi.org/10.7554/eLif...