Michele Partipilo
@michelepartipilo.bsky.social
Reposted by Michele Partipilo
How does Pseudomonas putida keep its redox balance steady across different metabolic states?
Our new preprint shows that two transhydrogenases -membrane-bound PntAB and soluble SthA - act together as a flexible, reversible system to control NAD(H)/NADP(H) ratios.
www.biorxiv.org/content/10.1...
Our new preprint shows that two transhydrogenases -membrane-bound PntAB and soluble SthA - act together as a flexible, reversible system to control NAD(H)/NADP(H) ratios.
www.biorxiv.org/content/10.1...
Integrated control of redox and energy metabolism by the membrane-bound and soluble transhydrogenases of Pseudomonas putida across metabolic regimes
Redox homeostasis is central to microbial physiology and stress adaptation, yet the functional roles of transhydrogenases remain poorly understood beyond a few organisms. In this study, we systematica...
www.biorxiv.org
November 9, 2025 at 7:59 PM
How does Pseudomonas putida keep its redox balance steady across different metabolic states?
Our new preprint shows that two transhydrogenases -membrane-bound PntAB and soluble SthA - act together as a flexible, reversible system to control NAD(H)/NADP(H) ratios.
www.biorxiv.org/content/10.1...
Our new preprint shows that two transhydrogenases -membrane-bound PntAB and soluble SthA - act together as a flexible, reversible system to control NAD(H)/NADP(H) ratios.
www.biorxiv.org/content/10.1...
Reposted by Michele Partipilo
250,000 people took part in the NO KINGS march in Chicago, with more than 7m marching in 2,700 locations US-wide, according to organisers @indivisible.org, as Americans increasingly resist Trump's authoritarianism.
October 19, 2025 at 8:26 AM
250,000 people took part in the NO KINGS march in Chicago, with more than 7m marching in 2,700 locations US-wide, according to organisers @indivisible.org, as Americans increasingly resist Trump's authoritarianism.
Reposted by Michele Partipilo
🚀 Thrilled to share our new Nature Communications article:
“A blueprint for designing the next-generation of synthetic C1 microbes”
We propose a framework to unlock sustainable, carbon-efficient biomanufacturing using non-canonical hosts.
🔗 www.nature.com/articles/s41... A short 🧵1/4
“A blueprint for designing the next-generation of synthetic C1 microbes”
We propose a framework to unlock sustainable, carbon-efficient biomanufacturing using non-canonical hosts.
🔗 www.nature.com/articles/s41... A short 🧵1/4
A blueprint for designing the next-generation of synthetic C1 microbes - Nature Communications
Synthetic one-carbon assimilation could contribute to a more sustainable and circular carbon economy, but much work in this field has focused on model microorganisms. Here the authors provide their perspective on the potential value of non-model microbes, and how that potential could be realised.
www.nature.com
October 8, 2025 at 9:10 AM
🚀 Thrilled to share our new Nature Communications article:
“A blueprint for designing the next-generation of synthetic C1 microbes”
We propose a framework to unlock sustainable, carbon-efficient biomanufacturing using non-canonical hosts.
🔗 www.nature.com/articles/s41... A short 🧵1/4
“A blueprint for designing the next-generation of synthetic C1 microbes”
We propose a framework to unlock sustainable, carbon-efficient biomanufacturing using non-canonical hosts.
🔗 www.nature.com/articles/s41... A short 🧵1/4
Reposted by Michele Partipilo
🚨 New paper in mBio!
We established strict formatotrophic & methylotrophic growth in engineered Pseudomonas putida via the reductive glycine pathway (rGlyP). journals.asm.org/doi/full/10....
Here’s a teaser of how we did it 🧵👇1/5
We established strict formatotrophic & methylotrophic growth in engineered Pseudomonas putida via the reductive glycine pathway (rGlyP). journals.asm.org/doi/full/10....
Here’s a teaser of how we did it 🧵👇1/5
Synthetic C1 metabolism in Pseudomonas putida enables strict formatotrophy and methylotrophy via the reductive glycine pathway | mBio
Soluble C1 feedstocks, such as formate and methanol, have gained attention as sustainable substrates for biotechnology, with the potential to reduce greenhouse gas emissions and reliance on sugar-based resources. Despite their promise, the metabolic ...
journals.asm.org
August 22, 2025 at 10:19 AM
🚨 New paper in mBio!
We established strict formatotrophic & methylotrophic growth in engineered Pseudomonas putida via the reductive glycine pathway (rGlyP). journals.asm.org/doi/full/10....
Here’s a teaser of how we did it 🧵👇1/5
We established strict formatotrophic & methylotrophic growth in engineered Pseudomonas putida via the reductive glycine pathway (rGlyP). journals.asm.org/doi/full/10....
Here’s a teaser of how we did it 🧵👇1/5
Reposted by Michele Partipilo
1/ 🚨 New preprint!
We've developed a robust IDMS-based LC-MS/MS workflow for high-throughput intracellular metabolomics in diverse bacteria. www.biorxiv.org/content/10.1...
#Metabolomics #MassSpec #SystemsBiology
We've developed a robust IDMS-based LC-MS/MS workflow for high-throughput intracellular metabolomics in diverse bacteria. www.biorxiv.org/content/10.1...
#Metabolomics #MassSpec #SystemsBiology
A Robust Isotope Ratio LC-MS/MS Workflow for High-Throughput Metabolic Profiling of Bacteria
Stable isotope dilution mass spectrometry (IDMS) has become a cornerstone of quantitative metabolomics, enabling accurate intracellular metabolite quantification across a range of biological systems. ...
www.biorxiv.org
August 12, 2025 at 9:50 AM
1/ 🚨 New preprint!
We've developed a robust IDMS-based LC-MS/MS workflow for high-throughput intracellular metabolomics in diverse bacteria. www.biorxiv.org/content/10.1...
#Metabolomics #MassSpec #SystemsBiology
We've developed a robust IDMS-based LC-MS/MS workflow for high-throughput intracellular metabolomics in diverse bacteria. www.biorxiv.org/content/10.1...
#Metabolomics #MassSpec #SystemsBiology
Reposted by Michele Partipilo
Now published in Microbial Biotechnology! enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10....
July 26, 2025 at 8:27 AM
Now published in Microbial Biotechnology! enviromicro-journals.onlinelibrary.wiley.com/doi/epdf/10....
Reposted by Michele Partipilo
The 5th edition of the #Pseudomonas grass roots meeting will take place on the 27th/28th of October 2025 in Denmark. This year, our group at DTU Biosustain will host 2 exciting days of networking, exchanging ideas and learning for early career-stage scientists working with Pseudomonas. 👇
July 9, 2025 at 11:21 AM
The 5th edition of the #Pseudomonas grass roots meeting will take place on the 27th/28th of October 2025 in Denmark. This year, our group at DTU Biosustain will host 2 exciting days of networking, exchanging ideas and learning for early career-stage scientists working with Pseudomonas. 👇
Great work by @oscarpuiggene.bsky.social from our @labnikel.bsky.social showing methanol assimilation in P. Putida via enhanced serine–threonine cycle, do not miss this read!
Check out our newest article led by @oscarpuiggene.bsky.social !
Implementing synthetic serine cycles in #Pseudomonas putida unlock methanol assimilation in our favorite bug w/carbon conservation, led by ÒscarPuiggené @labnikel.bsky.social · #SynBio #MetabolicEngineering #Methanol published in @cp-trendsbiotech.bsky.social
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
July 9, 2025 at 4:55 PM
Great work by @oscarpuiggene.bsky.social from our @labnikel.bsky.social showing methanol assimilation in P. Putida via enhanced serine–threonine cycle, do not miss this read!
Reposted by Michele Partipilo
Back from an inspiring week at #ME16 in our home #Copenhagen! 🌍
Engaging talks, exciting science, and amazing connections — we’re full of new ideas!
🎉 Big congrats to @giusifav.bsky.social for winning a poster award 🏆
🙌 And proud to have our PI @pabnik.bsky.social as co-chair of the event!
Engaging talks, exciting science, and amazing connections — we’re full of new ideas!
🎉 Big congrats to @giusifav.bsky.social for winning a poster award 🏆
🙌 And proud to have our PI @pabnik.bsky.social as co-chair of the event!
June 23, 2025 at 8:14 AM
Back from an inspiring week at #ME16 in our home #Copenhagen! 🌍
Engaging talks, exciting science, and amazing connections — we’re full of new ideas!
🎉 Big congrats to @giusifav.bsky.social for winning a poster award 🏆
🙌 And proud to have our PI @pabnik.bsky.social as co-chair of the event!
Engaging talks, exciting science, and amazing connections — we’re full of new ideas!
🎉 Big congrats to @giusifav.bsky.social for winning a poster award 🏆
🙌 And proud to have our PI @pabnik.bsky.social as co-chair of the event!
Reposted by Michele Partipilo
A promiscuous Bcd amino acid dehydrogenase promotes biofilm development in Bacillus subtilis
-in NPJ Biofilms and Microbiomes by David Ranava et al from Northwestern University / Mee-Ngan Yap, Arthur Prindle
www.nature.com/articles/s41...
-in NPJ Biofilms and Microbiomes by David Ranava et al from Northwestern University / Mee-Ngan Yap, Arthur Prindle
www.nature.com/articles/s41...
June 21, 2025 at 11:46 AM
A promiscuous Bcd amino acid dehydrogenase promotes biofilm development in Bacillus subtilis
-in NPJ Biofilms and Microbiomes by David Ranava et al from Northwestern University / Mee-Ngan Yap, Arthur Prindle
www.nature.com/articles/s41...
-in NPJ Biofilms and Microbiomes by David Ranava et al from Northwestern University / Mee-Ngan Yap, Arthur Prindle
www.nature.com/articles/s41...
Reposted by Michele Partipilo
The TOLERATE team met at DTU (Denmark) for a productive General Assembly.
New coordinator Pablo Ivan Nikel opened the meeting, presenting the transition and confirming that the project continues full speed ahead.
More info 👉 tolerate-eu-project.com
Subscribe 👉 eepurl.com/i6uufM
#EUProjects
New coordinator Pablo Ivan Nikel opened the meeting, presenting the transition and confirming that the project continues full speed ahead.
More info 👉 tolerate-eu-project.com
Subscribe 👉 eepurl.com/i6uufM
#EUProjects
June 2, 2025 at 4:33 PM
The TOLERATE team met at DTU (Denmark) for a productive General Assembly.
New coordinator Pablo Ivan Nikel opened the meeting, presenting the transition and confirming that the project continues full speed ahead.
More info 👉 tolerate-eu-project.com
Subscribe 👉 eepurl.com/i6uufM
#EUProjects
New coordinator Pablo Ivan Nikel opened the meeting, presenting the transition and confirming that the project continues full speed ahead.
More info 👉 tolerate-eu-project.com
Subscribe 👉 eepurl.com/i6uufM
#EUProjects
Reposted by Michele Partipilo
www.sciencenews.dk/en/designed-...
Piece from ScienceNews.dk on our glyoxylate and glycolate sensor strains
Piece from ScienceNews.dk on our glyoxylate and glycolate sensor strains
Designed bacteria can detect specific molecules
Researchers have developed a computer-guided method for designing and engineering bacteria that can detect the presence of specific molecules in the environment. This method will advance the use...
www.sciencenews.dk
May 26, 2025 at 12:43 PM
www.sciencenews.dk/en/designed-...
Piece from ScienceNews.dk on our glyoxylate and glycolate sensor strains
Piece from ScienceNews.dk on our glyoxylate and glycolate sensor strains
Reposted by Michele Partipilo
Solène’s 4.5 years of postdoctoral work in our lab (she started just before the corona pandemic) has now been published. A heroic effort that revealed -at the single molecule level- that ATP drives the dissociation of an ECF transporter complex under turnover conditions. See rdcu.be/elTKc
Single-molecule visualization of ATP-induced dynamics of the subunit composition of an ECF transporter complex under turnover conditions
Nature Communications - The association and dissociation dynamics of the ECF transporter complex for vitamin B12 are visualized by single-molecule FRET, highlighting the original transport...
rdcu.be
May 13, 2025 at 7:30 PM
Solène’s 4.5 years of postdoctoral work in our lab (she started just before the corona pandemic) has now been published. A heroic effort that revealed -at the single molecule level- that ATP drives the dissociation of an ECF transporter complex under turnover conditions. See rdcu.be/elTKc
Reposted by Michele Partipilo
Interested in engineering bacteria to turn #C1feedstocks into high-value chemicals for #bioproduction? Here is a thread summarizing our latest review on the roadblocks and how we might bio-hack our way past them 🧵 1/n academic.oup.com/femsre/advan...
Seven Critical Challenges in Synthetic One-Carbon Assimilation and Their Potential Solutions
Abstract. Synthetic C1 assimilation holds the promise of facilitating carbon capture while mitigating greenhouse gas emissions, yet practical implementatio
academic.oup.com
April 4, 2025 at 11:19 AM
Interested in engineering bacteria to turn #C1feedstocks into high-value chemicals for #bioproduction? Here is a thread summarizing our latest review on the roadblocks and how we might bio-hack our way past them 🧵 1/n academic.oup.com/femsre/advan...
Reposted by Michele Partipilo
Out now! Our latest paper where we show a "one-pot" labelling technique which allows for simultaneous mechanical and fluorescent readouts from a protein (Hsp90):
onlinelibrary.wiley.com/doi/10.1002/...
Congratulations to everyone who worked on this, especially Laura!
onlinelibrary.wiley.com/doi/10.1002/...
Congratulations to everyone who worked on this, especially Laura!
One‐pot dual protein labeling for simultaneous mechanical and fluorescent readouts in optical tweezers
Optical tweezers are widely used in the study of biological macromolecules but are limited by their one-directional probing capability, potentially missing critical conformational changes. Combining ...
onlinelibrary.wiley.com
March 18, 2025 at 6:08 PM
Out now! Our latest paper where we show a "one-pot" labelling technique which allows for simultaneous mechanical and fluorescent readouts from a protein (Hsp90):
onlinelibrary.wiley.com/doi/10.1002/...
Congratulations to everyone who worked on this, especially Laura!
onlinelibrary.wiley.com/doi/10.1002/...
Congratulations to everyone who worked on this, especially Laura!
Reposted by Michele Partipilo
Microbes like Cupriavidus necator naturally grow on #renewable feedstocks ♻️🧫 (CO₂, formate, hydrogen), yet their potential remains untapped due to the lack of efficient genetic tools... 1/2
www.cell.com/trends/biote...
www.cell.com/trends/biote...
March 16, 2025 at 3:40 PM
Microbes like Cupriavidus necator naturally grow on #renewable feedstocks ♻️🧫 (CO₂, formate, hydrogen), yet their potential remains untapped due to the lack of efficient genetic tools... 1/2
www.cell.com/trends/biote...
www.cell.com/trends/biote...
Reposted by Michele Partipilo
Our brand new CRISPR/Cas toolkit for genome editing of Cupriavidus necator is out🦠🧬
A joint effort w/ @dellavallesimo.bsky.social and Costas (and many more!)
Check 🧵 our long journey for developing a new state-of-the-art for the manipulation of this promising bug 1/n
www.cell.com/trends/biote...
A joint effort w/ @dellavallesimo.bsky.social and Costas (and many more!)
Check 🧵 our long journey for developing a new state-of-the-art for the manipulation of this promising bug 1/n
www.cell.com/trends/biote...
Streamlined and efficient genome editing in Cupriavidus necator H16 using an optimised SIBR-Cas system
This study developed two simple, efficient, and rapid genome editing tools termed
Self-splicing Intron-Based Riboswitch-Cas9 (SIBR-Cas9) and SIBR2.0-Cas12a, for editing
the genome of the industrially ...
www.cell.com
March 14, 2025 at 11:16 AM
Our brand new CRISPR/Cas toolkit for genome editing of Cupriavidus necator is out🦠🧬
A joint effort w/ @dellavallesimo.bsky.social and Costas (and many more!)
Check 🧵 our long journey for developing a new state-of-the-art for the manipulation of this promising bug 1/n
www.cell.com/trends/biote...
A joint effort w/ @dellavallesimo.bsky.social and Costas (and many more!)
Check 🧵 our long journey for developing a new state-of-the-art for the manipulation of this promising bug 1/n
www.cell.com/trends/biote...
Reposted by Michele Partipilo
The utilization of #C1-feedstocks, such as methanol, by non-model bacteria demands an in-depth analysis of design alternatives, a modular strategy to identify bottlenecks and efficient solutions within the host #metabolism of the chosen host...
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Systematic engineering of synthetic serine cycles in Pseudomonas putida uncovers emergent topologies for methanol assimilation
The urgent need for a circular carbon economy has driven research into sustainable substrates, including one-carbon (C1) compounds. The non-pathogenic soil bacterium Pseudomonas putida is a promising ...
www.biorxiv.org
March 9, 2025 at 10:51 AM
The utilization of #C1-feedstocks, such as methanol, by non-model bacteria demands an in-depth analysis of design alternatives, a modular strategy to identify bottlenecks and efficient solutions within the host #metabolism of the chosen host...
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
With great collaborations come great outcomes! A fine example from @eorsi.bsky.social from @pabnik.bsky.social's lab et collaborators of how to design, build and develop cell biosensors for value-added chemicals (related to synthetic C1 assimilation, of course)! 🧪
We just #published an end-to-end work for the computer-aided design💻, construction🛠️, validation✅, characterization🔬 & application💪 of #auxotroph metabolic sensors, the work-horses of in vivo growth-coupled selection screening systems-using glycolate as test case. 🧵👇1/
www.nature.com/articles/s41...
www.nature.com/articles/s41...
March 5, 2025 at 8:01 AM
With great collaborations come great outcomes! A fine example from @eorsi.bsky.social from @pabnik.bsky.social's lab et collaborators of how to design, build and develop cell biosensors for value-added chemicals (related to synthetic C1 assimilation, of course)! 🧪
Reposted by Michele Partipilo
Computation-aided designs enable developing auxotrophic #metabolic #sensors for wide-range glyoxylate and glycolate detection—spearheaded by Enrico Orsi w/a choral assembly of cool collaborators @naturecomms.bsky.social #SynBio #OpenAccess
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Computation-aided designs enable developing auxotrophic metabolic sensors for wide-range glyoxylate and glycolate detection - Nature Communications
Auxotrophic metabolic sensors (AMS) are vital for bioengineering but are often time-consuming to develop. Here, the authors present a workflow for designing versatile AMS, demonstrating their use for ...
www.nature.com
March 4, 2025 at 1:23 PM
Computation-aided designs enable developing auxotrophic #metabolic #sensors for wide-range glyoxylate and glycolate detection—spearheaded by Enrico Orsi w/a choral assembly of cool collaborators @naturecomms.bsky.social #SynBio #OpenAccess
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Michele Partipilo
Preprint alert🚨Systematic #engineering of synthetic serine cycles in #Pseudomonas putida uncovers emergent topologies for #methanol assimilation · @biorxivpreprint.bsky.social led by Òscar Puiggené #SynBio #Methylotrophy #CircularEconomy
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
February 21, 2025 at 7:03 AM
Preprint alert🚨Systematic #engineering of synthetic serine cycles in #Pseudomonas putida uncovers emergent topologies for #methanol assimilation · @biorxivpreprint.bsky.social led by Òscar Puiggené #SynBio #Methylotrophy #CircularEconomy
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Michele Partipilo
Super excited to see my main PhD project finally out in @naturemicrobiol.bsky.social www.nature.com/articles/s41.... We show for the first time that a synthetic and engineered pathway can indeed exceed nature at one-carbon fixation.
One-carbon fixation via the synthetic reductive glycine pathway exceeds yield of the Calvin cycle - Nature Microbiology
An engineered one-carbon-fixation pathway increases biomass yields of Cupriavidus necator compared with the Calvin cycle and can support future, sustainable bio-based production.
www.nature.com
February 27, 2025 at 10:34 AM
Super excited to see my main PhD project finally out in @naturemicrobiol.bsky.social www.nature.com/articles/s41.... We show for the first time that a synthetic and engineered pathway can indeed exceed nature at one-carbon fixation.
One-carbon fixation at the next level! A piece of work that opens up new scenarios for engineering bacteria for sustainable biomanufacturing! ⚗️🦠♻️
Super excited to see my main PhD project finally out in @naturemicrobiol.bsky.social www.nature.com/articles/s41.... We show for the first time that a synthetic and engineered pathway can indeed exceed nature at one-carbon fixation.
One-carbon fixation via the synthetic reductive glycine pathway exceeds yield of the Calvin cycle - Nature Microbiology
An engineered one-carbon-fixation pathway increases biomass yields of Cupriavidus necator compared with the Calvin cycle and can support future, sustainable bio-based production.
www.nature.com
March 1, 2025 at 10:35 AM
One-carbon fixation at the next level! A piece of work that opens up new scenarios for engineering bacteria for sustainable biomanufacturing! ⚗️🦠♻️
Reposted by Michele Partipilo
New: Using F-tractin to visualize #actin in living cells? Dmitry Shatskiy, Athul Sivan, Roland Wedlich-Söldner, and Alexander Belyy @alexanderbelyy.bsky.social determined the F-tractin-F-actin structure and showed that this probe is nearly identical to #Lifeact. https://buff.ly/3EIqAur
#cytoskeleton
#cytoskeleton
February 10, 2025 at 5:59 PM
New: Using F-tractin to visualize #actin in living cells? Dmitry Shatskiy, Athul Sivan, Roland Wedlich-Söldner, and Alexander Belyy @alexanderbelyy.bsky.social determined the F-tractin-F-actin structure and showed that this probe is nearly identical to #Lifeact. https://buff.ly/3EIqAur
#cytoskeleton
#cytoskeleton
Reposted by Michele Partipilo
