Mapping protein interactions requires sensitive mass spectrometry. Here, the authors show that the Orbitrap Astral, combined with optimized chromatography, improves detection of low-abundance crosslin...

dplyr but make it bussin fr fr no cap. Contribute to hadley/genzplyr development by creating an account on GitHub.

Communications Chemistry, Published online: 13 August 2025; doi:10.1038/s42004-025-01644-6Cross-linking mass spectrometry faces challenges due to low reaction efficiencies and complex matrices in protein–protein interaction (PPI) studies. Here, the authors enhance an Azide-A-DSBSO-based in vivo crosslinking workflow with affinity enrichment and size exclusion chromatography, yielding over 5000 crosslinks from K562 cells and generating a comprehensive PPI network.

Crosslinking proteomics is not really my thing. I've done it a few times over the years and I've personally had success with in vitro expe...

Communications Chemistry, Published online: 23 June 2025; doi:10.1038/s42004-025-01568-1Crosslinking mass spectrometry (XL-MS) has become a crucial tool for studying protein structures and interactions in their native environments, however, the current cleavable linkers are fraught with practical limitations. Here, the authors synthesize and characterize DiSPASO, a novel lysine-reactive, MS-cleavable, and membrane-permeable crosslinker with an alkyne-based click chemistry handle for affinity enrichment, showing potential for improving protein-protein interaction mapping.

While LC retention time prediction of peptides and their modifications has proven useful, widespread adoption and optimal performance are hindered by variations in experimental parameters. These varia...

MS Annika is a crosslink search engine based on MS Amanda, aimed at identifying crosslinks of cleavable and non-cleavable crosslinkers from MS2 and MS3 spectra. - hgb-bin-proteomics/MSAnnika