For over a century, evolutionary biologists have been motivated to understand the mechanisms through which organisms adapt to their environments. Coloration and pigmentation are remarkably variable wi...

Caterpillars (larval Lepidoptera) are one of the most ecologically and evolutionarily significant taxa on Earth. As both feeders and food, they shape the dynamics of enumerate ecosystems on land. Key ...

Abstract. Mosquito-borne diseases pose a dire threat to humanity, claiming over 700,000 lives annually. At the local scale, the interplay between several e

A comprehensive single-nucleus RNA-seq atlas of >367,000 nuclei from male and female Aedes aegypti mosquitoes reveals sexual dimorphism in sensory systems and brain cell types and widespread co-expres...

Abstract. Understanding the drivers of biodiversity is a central goal in evolutionary biology. In particular, sexual selection has long been proposed as a

Journal of Comparative Physiology A - Mate preference learning, where individuals learn to prefer or avoid specific phenotypes during mate selection, is pervasive across animal taxa and influences...

Mulhair et al. uncover a functional bicoid in non-cyclorrhaphan flies, pushing the gene's origin back by ∼20 million years. Reassessing the Diptera phylogeny using the largest dataset to date permits ...

Online Applications – Accademia Nazionale dei Lincei

Abstract. Mapping genotypes to phenotypes is a fundamental goal in biology. Phylogenetic Genotype to Phenotype mapping methods are a relatively new set of

Advances in sequencing technologies have enabled transcriptional profiling of previously understudied yet critical species, such as mosquitoes. Here, we present a protocol for multiplex whole-mount RNA fluorescence in situ hybridization combined with immunohistochemistry in the Anopheles gambiae brain. We describe steps for dissection, fixation, probe hybridization, hybridization chain reaction (HCR) probe detection, and primary antibody application. We then detail procedures for tissue preparat...

Molecular evolution studies often rely on single-copy orthologs. This study presents OrthoSNAP, an algorithm that identifies and extracts additional single-copy orthologs nested within larger gene fam...

Data and code are essential for ensuring the credibility of scientific results and facilitating reproducibility, areas in which journal sharing policies play a crucial role. However, in ecology and ev...

Abstract. Sensory adaptation is widely hypothesized to drive ecological speciation, yet empirical evidence from natural populations undergoing early stage

Background Variation within the prokaryotic pangenome is not random, and natural selection that favours particular combinations of genes appears to dominate over random drift. What is less clear is wh...

Single-cell RNA-sequencing revolutionized our approach of transcriptomic studies, enabling to analyze gene expression across cell type in a tissue. Here we introduce an optimized cell dissociation and...

Communication is crucial to social life, and in ants, it is mediated primarily through olfaction. Ants have more odorant receptor (OR) genes than any other group of insects, generated through tandem d...