Josh Tycko
@joshtycko.com
Synthetic biology and gene regulation
Aka DJ Fishing Expedition
Aka DJ Fishing Expedition
Reposted by Josh Tycko
In a medical breakthrough, a team including IGI’s
@urnov.bsky.social & @giannikopoulosp.bsky.social created an on-demand #CRISPR therapy for an infant with a deadly gene mutation — developed, approved, and delivered to the patient in just 6 months.
Read more: ow.ly/G0Bg50VTonC
#RareDisease 🧬
@urnov.bsky.social & @giannikopoulosp.bsky.social created an on-demand #CRISPR therapy for an infant with a deadly gene mutation — developed, approved, and delivered to the patient in just 6 months.
Read more: ow.ly/G0Bg50VTonC
#RareDisease 🧬
May 15, 2025 at 5:04 PM
In a medical breakthrough, a team including IGI’s
@urnov.bsky.social & @giannikopoulosp.bsky.social created an on-demand #CRISPR therapy for an infant with a deadly gene mutation — developed, approved, and delivered to the patient in just 6 months.
Read more: ow.ly/G0Bg50VTonC
#RareDisease 🧬
@urnov.bsky.social & @giannikopoulosp.bsky.social created an on-demand #CRISPR therapy for an infant with a deadly gene mutation — developed, approved, and delivered to the patient in just 6 months.
Read more: ow.ly/G0Bg50VTonC
#RareDisease 🧬
Reposted by Josh Tycko
I am elated to share that our manuscript describing Variant-EFFECTS, a high-throughput technology we developed to precisely and quantitatively measure the effects of CRISPR-mediated edits on gene expression, is now published at @cellpress.bsky.social: authors.elsevier.com/c/1kxgiL7PXu...
authors.elsevier.com
April 17, 2025 at 6:20 PM
I am elated to share that our manuscript describing Variant-EFFECTS, a high-throughput technology we developed to precisely and quantitatively measure the effects of CRISPR-mediated edits on gene expression, is now published at @cellpress.bsky.social: authors.elsevier.com/c/1kxgiL7PXu...
Reposted by Josh Tycko
What contributes to human specific neural specification? Check out these two wonderful preprints from a wonderful scientist Ava carter! 1. www.biorxiv.org/content/10.1...; 2. www.biorxiv.org/content/10.1...
Stay tuned! third preprint is on its way!
Stay tuned! third preprint is on its way!
Human-chimpanzee tetraploid system defines mechanisms of species-specific neural gene regulation
A major challenge in human evolutionary biology is to pinpoint genetic differences that underlie human-specific traits, such as increased neuron number and differences in cognitive behaviors. We used ...
www.biorxiv.org
April 2, 2025 at 7:24 PM
What contributes to human specific neural specification? Check out these two wonderful preprints from a wonderful scientist Ava carter! 1. www.biorxiv.org/content/10.1...; 2. www.biorxiv.org/content/10.1...
Stay tuned! third preprint is on its way!
Stay tuned! third preprint is on its way!
Reposted by Josh Tycko
How Multiplexed Gene Fragments are Expanding What’s Possible in Molecular Biology!
@twistbioscience.com
@joshtycko.com
Moving Beyond 300 Base Pairs:
@twistbioscience.com
@joshtycko.com
Moving Beyond 300 Base Pairs:
Moving Beyond 300 Base Pairs | Twist Bioscience
In a recent episode of the podcast series Lab Talk by The Scientist, guest speaker Josh Tycko’s excitement was infectious. Tycko, a postdoctoral researcher at Harvard Medical School, spoke about how f...
www.twistbioscience.com
March 5, 2025 at 2:21 PM
How Multiplexed Gene Fragments are Expanding What’s Possible in Molecular Biology!
@twistbioscience.com
@joshtycko.com
Moving Beyond 300 Base Pairs:
@twistbioscience.com
@joshtycko.com
Moving Beyond 300 Base Pairs:
Our sun and planets form a tiny nucleus surrounded by the Oort cloud, a thick icy shell that has never been directly observed but is thought to provide a continuous source of comets
Other stellar systems can crash through the Oort cloud, and it could happen again in as little as 1-10 million years
Other stellar systems can crash through the Oort cloud, and it could happen again in as little as 1-10 million years
January 13, 2025 at 5:04 PM
Our sun and planets form a tiny nucleus surrounded by the Oort cloud, a thick icy shell that has never been directly observed but is thought to provide a continuous source of comets
Other stellar systems can crash through the Oort cloud, and it could happen again in as little as 1-10 million years
Other stellar systems can crash through the Oort cloud, and it could happen again in as little as 1-10 million years
Reposted by Josh Tycko
Impressive study from Alex Stark lab identifying 3 new types of silencer elements in Drosophila
New TF binds a particular isolated motif in non-accessible sites to recruit G9a. Deletion of these elements can lead to upregulation of nearby gene
www.sciencedirect.com/science/arti...
New TF binds a particular isolated motif in non-accessible sites to recruit G9a. Deletion of these elements can lead to upregulation of nearby gene
www.sciencedirect.com/science/arti...
November 20, 2024 at 9:34 PM
Impressive study from Alex Stark lab identifying 3 new types of silencer elements in Drosophila
New TF binds a particular isolated motif in non-accessible sites to recruit G9a. Deletion of these elements can lead to upregulation of nearby gene
www.sciencedirect.com/science/arti...
New TF binds a particular isolated motif in non-accessible sites to recruit G9a. Deletion of these elements can lead to upregulation of nearby gene
www.sciencedirect.com/science/arti...
Reposted by Josh Tycko
www.nature.com/articles/s41...
Single-molecule states link transcription factor binding to gene expression
Deep insights into multiple facets of TF binding, accessibility & expression with biophysical models of single molecule data by Ben Doughty, Michaela Hinks, Bintu & Greenleaf labs
Single-molecule states link transcription factor binding to gene expression
Deep insights into multiple facets of TF binding, accessibility & expression with biophysical models of single molecule data by Ben Doughty, Michaela Hinks, Bintu & Greenleaf labs
Single-molecule states link transcription factor binding to gene expression - Nature
A study uses single-molecule footprinting to measure protein occupancy at regulatory elements on individual molecules in human cells and describes how different properties of transcription factor bind...
www.nature.com
November 21, 2024 at 4:15 PM
www.nature.com/articles/s41...
Single-molecule states link transcription factor binding to gene expression
Deep insights into multiple facets of TF binding, accessibility & expression with biophysical models of single molecule data by Ben Doughty, Michaela Hinks, Bintu & Greenleaf labs
Single-molecule states link transcription factor binding to gene expression
Deep insights into multiple facets of TF binding, accessibility & expression with biophysical models of single molecule data by Ben Doughty, Michaela Hinks, Bintu & Greenleaf labs
Deep review of an accelerating area in genome engineering - large insertions & deletions. The authors here really dug up what's known and nicely synthesized it.
It ends with a fun question: "Do we need most of our DNA at all, or could we delete 90% of it without any loss of function?"
It ends with a fun question: "Do we need most of our DNA at all, or could we delete 90% of it without any loss of function?"
Structural variants are ripe for interrogation using genome engineering. Jonas Koeppel Juliane Weller Thomas Vanderstichele (Wellcome Sanger Institute) and I review technology progress, insights gained to date, and challenges and promise for the road ahead. www.nature.com/articles/s41588-024-01981-7
Engineering structural variants to interrogate genome function - Nature Genetics
Structural variations (SVs) impact gene expression, genome stability and disease susceptibility. This Review discusses recent advances in genome-engineering tools that enable precise SV generation and...
www.nature.com
November 19, 2024 at 12:26 AM
Deep review of an accelerating area in genome engineering - large insertions & deletions. The authors here really dug up what's known and nicely synthesized it.
It ends with a fun question: "Do we need most of our DNA at all, or could we delete 90% of it without any loss of function?"
It ends with a fun question: "Do we need most of our DNA at all, or could we delete 90% of it without any loss of function?"
New here and excited to share our new paper on transcriptional effectors! Interested to discuss context-specificity of gene regulation? Or how to get CRISPRi/a working in new contexts? 👋
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Development of compact transcriptional effectors using high-throughput measurements in diverse contexts - Nature Biotechnology
Improved effectors for CRISPRi/CRISPRa are developed following high-throughput screening of transcriptional domains.
www.nature.com
November 18, 2024 at 3:16 AM
New here and excited to share our new paper on transcriptional effectors! Interested to discuss context-specificity of gene regulation? Or how to get CRISPRi/a working in new contexts? 👋
www.nature.com/articles/s41...
www.nature.com/articles/s41...