Michael Montgomery
@michaeltmont.bsky.social
Stanford PhD candidate in the Engreitz Lab. Passionate about engineering gene regulation and high-throughput tech dev.
Had a lot of fun writing this “tools of the trade” highlight for our Variant-EFFECTS technology. Check it out! 🛠️
New online! Measuring the effects of regulatory variants in an endogenous context
Measuring the effects of regulatory variants in an endogenous context
Nature Reviews Genetics, Published online: 11 June 2025; doi:10.1038/s41576-025-00863-wIn this Tools of the Trade article, Michael Montgomery presents Variant-EFFECTS, a high-throughput and generalizable approach that combines prime editing, flow cytometry, sequencing, and computational analysis to quantify the effects of regulatory variants.
www.nature.com
June 11, 2025 at 2:01 PM
Had a lot of fun writing this “tools of the trade” highlight for our Variant-EFFECTS technology. Check it out! 🛠️
Very cool work! I'm particularly excited about the integration of multiple models/prediction heads in this approach, given how many aspects of gene regulation likely need to be considered to achieve a specific quantitative design goal.
Our preprint on designing and editing cis-regulatory elements using Ledidi is out! Ledidi turns *any* ML model (or set of models) into a designer of edits to DNA sequences that induce desired characteristics.
Preprint: www.biorxiv.org/content/10.1...
GitHub: github.com/jmschrei/led...
Preprint: www.biorxiv.org/content/10.1...
GitHub: github.com/jmschrei/led...
Programmatic design and editing of cis-regulatory elements
The development of modern genome editing tools has enabled researchers to make such edits with high precision but has left unsolved the problem of designing these edits. As a solution, we propose Ledi...
www.biorxiv.org
April 24, 2025 at 6:06 PM
Very cool work! I'm particularly excited about the integration of multiple models/prediction heads in this approach, given how many aspects of gene regulation likely need to be considered to achieve a specific quantitative design goal.
I am elated to share that our manuscript describing Variant-EFFECTS, a high-throughput technology we developed to precisely and quantitatively measure the effects of CRISPR-mediated edits on gene expression, is now published at @cellpress.bsky.social: authors.elsevier.com/c/1kxgiL7PXu...
authors.elsevier.com
April 17, 2025 at 6:20 PM
I am elated to share that our manuscript describing Variant-EFFECTS, a high-throughput technology we developed to precisely and quantitatively measure the effects of CRISPR-mediated edits on gene expression, is now published at @cellpress.bsky.social: authors.elsevier.com/c/1kxgiL7PXu...