Duxin lab
@duxinlab.bsky.social
Diving into genome replication and stability research
Collaborating shoulder to shoulder with Xenopus frogs 🐸 at @bric-ucph.bsky.social, @ucph.bsky.social
Collaborating shoulder to shoulder with Xenopus frogs 🐸 at @bric-ucph.bsky.social, @ucph.bsky.social
Pinned
Duxin lab
@duxinlab.bsky.social
· Aug 22
It is not always safe to repair DNA Damage. Sometimes, cells "just bypass it".
During DNA replication, repair of lesions on DNA can be dangerous. Cells instead "tolerate" DNA damage and focus on finishing replication.
Read our review and find out why and how: doi.org/10.1016/j.ti...
During DNA replication, repair of lesions on DNA can be dangerous. Cells instead "tolerate" DNA damage and focus on finishing replication.
Read our review and find out why and how: doi.org/10.1016/j.ti...
Excited to have contributed to this new @cp-molcell.bsky.social paper on how PARP1 auto-modification promotes faithful Okazaki fragment processing and limits replication fork speed.
A big congrats to Jonas for driving this work forward, great to see the results out!
👉 10.1016/j.molcel.2025.09.006
A big congrats to Jonas for driving this work forward, great to see the results out!
👉 10.1016/j.molcel.2025.09.006
October 3, 2025 at 6:21 PM
Excited to have contributed to this new @cp-molcell.bsky.social paper on how PARP1 auto-modification promotes faithful Okazaki fragment processing and limits replication fork speed.
A big congrats to Jonas for driving this work forward, great to see the results out!
👉 10.1016/j.molcel.2025.09.006
A big congrats to Jonas for driving this work forward, great to see the results out!
👉 10.1016/j.molcel.2025.09.006
BRIC Day with the theme collide across each other — all about sparking new intra-institute collaborations.
Kickoff with a keynote from Rasmus Rabøl (Klifo) and a buzzing poster session full of science-in-the-making!
@bric-ucph.bsky.social
Kickoff with a keynote from Rasmus Rabøl (Klifo) and a buzzing poster session full of science-in-the-making!
@bric-ucph.bsky.social
October 2, 2025 at 4:45 PM
BRIC Day with the theme collide across each other — all about sparking new intra-institute collaborations.
Kickoff with a keynote from Rasmus Rabøl (Klifo) and a buzzing poster session full of science-in-the-making!
@bric-ucph.bsky.social
Kickoff with a keynote from Rasmus Rabøl (Klifo) and a buzzing poster session full of science-in-the-making!
@bric-ucph.bsky.social
Today we swapped pipettes for putters.
Day 2 retreat highlight: realizing that hitting the fairway is way harder than hitting a replication fork 😅
Day 2 retreat highlight: realizing that hitting the fairway is way harder than hitting a replication fork 😅
September 18, 2025 at 5:22 PM
Today we swapped pipettes for putters.
Day 2 retreat highlight: realizing that hitting the fairway is way harder than hitting a replication fork 😅
Day 2 retreat highlight: realizing that hitting the fairway is way harder than hitting a replication fork 😅
Off to Nord Sjælland with the Miller lab @tcr-miller.bsky.social for our joint retreat! Exciting brainstorming, bonding, and a little hygge ahead.
First challenge: design your next @natureportfolio.nature.com article 🚀
First challenge: design your next @natureportfolio.nature.com article 🚀
September 17, 2025 at 5:52 PM
Off to Nord Sjælland with the Miller lab @tcr-miller.bsky.social for our joint retreat! Exciting brainstorming, bonding, and a little hygge ahead.
First challenge: design your next @natureportfolio.nature.com article 🚀
First challenge: design your next @natureportfolio.nature.com article 🚀
Reposted by Duxin lab
What makes #cshldnarep special isn’t just the cutting-edge, top-notch science; it’s the chance to meet the authors of the papers we build on every day, exchange ideas, and feel part of this community.
Heading home exhausted, grateful, full of ideas and renewed motivation to push my project forward!
Heading home exhausted, grateful, full of ideas and renewed motivation to push my project forward!
September 8, 2025 at 9:04 AM
What makes #cshldnarep special isn’t just the cutting-edge, top-notch science; it’s the chance to meet the authors of the papers we build on every day, exchange ideas, and feel part of this community.
Heading home exhausted, grateful, full of ideas and renewed motivation to push my project forward!
Heading home exhausted, grateful, full of ideas and renewed motivation to push my project forward!
Our PhD student @martaolivasantiago.bsky.social and postdoc @amitash.bsky.social presented their posters on #cshldnarep, turning data into an insightful dialogue.
Huge thanks to everyone who stopped by our poster!
Huge thanks to everyone who stopped by our poster!
September 5, 2025 at 1:49 PM
Our PhD student @martaolivasantiago.bsky.social and postdoc @amitash.bsky.social presented their posters on #cshldnarep, turning data into an insightful dialogue.
Huge thanks to everyone who stopped by our poster!
Huge thanks to everyone who stopped by our poster!
This week we’re at CSHL #cshldnarep where the DNA replication & genome maintenance community has gathered for a week of science, discussions, and late-night brainstorming.
September 4, 2025 at 2:57 PM
This week we’re at CSHL #cshldnarep where the DNA replication & genome maintenance community has gathered for a week of science, discussions, and late-night brainstorming.
Reposted by Duxin lab
Sometimes DNA replication hits a 🛑 roadblock.
Cells don’t stop; they take clever detours (DNA damage tolerance) 🚗💨 to keep genomes intact.
That’s the focus of our new review in @cp-trendsbiochem.bsky.social:
“Just bypass it: mechanisms of DNA damage tolerance”
🔗 doi.org/10.1016/j.ti...
Cells don’t stop; they take clever detours (DNA damage tolerance) 🚗💨 to keep genomes intact.
That’s the focus of our new review in @cp-trendsbiochem.bsky.social:
“Just bypass it: mechanisms of DNA damage tolerance”
🔗 doi.org/10.1016/j.ti...
August 25, 2025 at 3:01 PM
Sometimes DNA replication hits a 🛑 roadblock.
Cells don’t stop; they take clever detours (DNA damage tolerance) 🚗💨 to keep genomes intact.
That’s the focus of our new review in @cp-trendsbiochem.bsky.social:
“Just bypass it: mechanisms of DNA damage tolerance”
🔗 doi.org/10.1016/j.ti...
Cells don’t stop; they take clever detours (DNA damage tolerance) 🚗💨 to keep genomes intact.
That’s the focus of our new review in @cp-trendsbiochem.bsky.social:
“Just bypass it: mechanisms of DNA damage tolerance”
🔗 doi.org/10.1016/j.ti...
Reposted by Duxin lab
New cool research alert!
Tita Sixma #RepliFate lab uncovered the preferred exo-cleavage mechanism by which USP1/UAF1 can cleave ubiquitin chains on polyubiquitinated PCNA and temporarily enrich for monoubiquitinated PCNA.
Read the full study: doi.org/10.1038/s414...
Tita Sixma #RepliFate lab uncovered the preferred exo-cleavage mechanism by which USP1/UAF1 can cleave ubiquitin chains on polyubiquitinated PCNA and temporarily enrich for monoubiquitinated PCNA.
Read the full study: doi.org/10.1038/s414...
USP1/UAF1 targets polyubiquitinated PCNA with an exo-cleavage mechanism that can temporarily enrich for monoubiquitinated PCNA - Nature Communications
DNA damage tolerance is regulated by ubiquitination of PCNA. Here, the authors present kinetic and structural studies showing that USP1/UAF1 prefers trimming K63- and K48-ubiquitin chains down over cl...
doi.org
August 22, 2025 at 11:23 AM
New cool research alert!
Tita Sixma #RepliFate lab uncovered the preferred exo-cleavage mechanism by which USP1/UAF1 can cleave ubiquitin chains on polyubiquitinated PCNA and temporarily enrich for monoubiquitinated PCNA.
Read the full study: doi.org/10.1038/s414...
Tita Sixma #RepliFate lab uncovered the preferred exo-cleavage mechanism by which USP1/UAF1 can cleave ubiquitin chains on polyubiquitinated PCNA and temporarily enrich for monoubiquitinated PCNA.
Read the full study: doi.org/10.1038/s414...
It is not always safe to repair DNA Damage. Sometimes, cells "just bypass it".
During DNA replication, repair of lesions on DNA can be dangerous. Cells instead "tolerate" DNA damage and focus on finishing replication.
Read our review and find out why and how: doi.org/10.1016/j.ti...
During DNA replication, repair of lesions on DNA can be dangerous. Cells instead "tolerate" DNA damage and focus on finishing replication.
Read our review and find out why and how: doi.org/10.1016/j.ti...
August 22, 2025 at 11:48 AM
It is not always safe to repair DNA Damage. Sometimes, cells "just bypass it".
During DNA replication, repair of lesions on DNA can be dangerous. Cells instead "tolerate" DNA damage and focus on finishing replication.
Read our review and find out why and how: doi.org/10.1016/j.ti...
During DNA replication, repair of lesions on DNA can be dangerous. Cells instead "tolerate" DNA damage and focus on finishing replication.
Read our review and find out why and how: doi.org/10.1016/j.ti...
Reposted by Duxin lab
Just published!
Lecona (@emlecona.bsky.social ) & Méndez #RepliFate labs show that VCP/p97 removes Pol α/primase from chromatin to avoid excess ATR activation during normal #DNAreplication
VCP/p97 = checkpoint gatekeeper!
Read the full study: doi.org/10.1038/s414...
Lecona (@emlecona.bsky.social ) & Méndez #RepliFate labs show that VCP/p97 removes Pol α/primase from chromatin to avoid excess ATR activation during normal #DNAreplication
VCP/p97 = checkpoint gatekeeper!
Read the full study: doi.org/10.1038/s414...
DNA polymerase α/primase extraction from chromatin by VCP/p97 restricts ATR activation during unperturbed DNA replication - Nature Communications
The replication stress response ensures genomic stability throughout DNA duplication. Here, the authors show that VCP/p97 extracts the DNA polymerase α/Primase complex from chromatin, preventing exces...
doi.org
July 3, 2025 at 12:03 PM
Just published!
Lecona (@emlecona.bsky.social ) & Méndez #RepliFate labs show that VCP/p97 removes Pol α/primase from chromatin to avoid excess ATR activation during normal #DNAreplication
VCP/p97 = checkpoint gatekeeper!
Read the full study: doi.org/10.1038/s414...
Lecona (@emlecona.bsky.social ) & Méndez #RepliFate labs show that VCP/p97 removes Pol α/primase from chromatin to avoid excess ATR activation during normal #DNAreplication
VCP/p97 = checkpoint gatekeeper!
Read the full study: doi.org/10.1038/s414...
🎉 Congratulations to SUND's new professors!
The Faculty of Health and Medical Sciences, @ucph.bsky.social celebrated 23 newly appointed professors.
Through their dedicated work, they contribute every day to the development of the university, the healthcare system, and society at large.
The Faculty of Health and Medical Sciences, @ucph.bsky.social celebrated 23 newly appointed professors.
Through their dedicated work, they contribute every day to the development of the university, the healthcare system, and society at large.
June 23, 2025 at 8:06 AM
🎉 Congratulations to SUND's new professors!
The Faculty of Health and Medical Sciences, @ucph.bsky.social celebrated 23 newly appointed professors.
Through their dedicated work, they contribute every day to the development of the university, the healthcare system, and society at large.
The Faculty of Health and Medical Sciences, @ucph.bsky.social celebrated 23 newly appointed professors.
Through their dedicated work, they contribute every day to the development of the university, the healthcare system, and society at large.
🌿 Off to Hillerød for the 2025 MoMeD Summer School!
Our PI Julien Duxin -as head of the MoMeD PhD Programme at @ucph.bsky.social- joins our PhD students and many others for 2 packed days of science, networking, and inspiration.
Looking forward to connecting and exchanging ideas across fields!
Our PI Julien Duxin -as head of the MoMeD PhD Programme at @ucph.bsky.social- joins our PhD students and many others for 2 packed days of science, networking, and inspiration.
Looking forward to connecting and exchanging ideas across fields!
June 12, 2025 at 8:19 AM
🌿 Off to Hillerød for the 2025 MoMeD Summer School!
Our PI Julien Duxin -as head of the MoMeD PhD Programme at @ucph.bsky.social- joins our PhD students and many others for 2 packed days of science, networking, and inspiration.
Looking forward to connecting and exchanging ideas across fields!
Our PI Julien Duxin -as head of the MoMeD PhD Programme at @ucph.bsky.social- joins our PhD students and many others for 2 packed days of science, networking, and inspiration.
Looking forward to connecting and exchanging ideas across fields!
Reposted by Duxin lab
Frogs in the lab? Yep! 🐸
This week, RepliFate PhD students are diving into the Xenopus egg extract system at the @duxinlab.bsky.social (@bric-ucph.bsky.social, @ucph.bsky.social) -where test tubes meet frog magic to unravel the mysteries of DNA replication and genome integrity.
This week, RepliFate PhD students are diving into the Xenopus egg extract system at the @duxinlab.bsky.social (@bric-ucph.bsky.social, @ucph.bsky.social) -where test tubes meet frog magic to unravel the mysteries of DNA replication and genome integrity.
June 5, 2025 at 2:16 PM
Frogs in the lab? Yep! 🐸
This week, RepliFate PhD students are diving into the Xenopus egg extract system at the @duxinlab.bsky.social (@bric-ucph.bsky.social, @ucph.bsky.social) -where test tubes meet frog magic to unravel the mysteries of DNA replication and genome integrity.
This week, RepliFate PhD students are diving into the Xenopus egg extract system at the @duxinlab.bsky.social (@bric-ucph.bsky.social, @ucph.bsky.social) -where test tubes meet frog magic to unravel the mysteries of DNA replication and genome integrity.
Our lab’s a little more crowded (and a lot more curious) this week! 🧠🐸
We’re thrilled to welcome @RepliFate students for a hands-on course in Xenopus egg extracts.
They're diving into the Xenopus world and learning how this powerful system helps us explore DNA replication and genome stability.
We’re thrilled to welcome @RepliFate students for a hands-on course in Xenopus egg extracts.
They're diving into the Xenopus world and learning how this powerful system helps us explore DNA replication and genome stability.
June 4, 2025 at 6:43 AM
Our lab’s a little more crowded (and a lot more curious) this week! 🧠🐸
We’re thrilled to welcome @RepliFate students for a hands-on course in Xenopus egg extracts.
They're diving into the Xenopus world and learning how this powerful system helps us explore DNA replication and genome stability.
We’re thrilled to welcome @RepliFate students for a hands-on course in Xenopus egg extracts.
They're diving into the Xenopus world and learning how this powerful system helps us explore DNA replication and genome stability.
🎯 A tenure-track group leader position in translational cancer research is now open at Finsen Laboratory.
Come join an excellent and friendly research environment combining both the Rigshospitalet and the KU @ucph.bsky.social affiliated groups
candidate.hr-manager.net/ApplicationI...
Come join an excellent and friendly research environment combining both the Rigshospitalet and the KU @ucph.bsky.social affiliated groups
candidate.hr-manager.net/ApplicationI...
Tenure-track Research Group leader position in translational cancer research at the Finsen Laboratory, Rigshospitalet, Copenhagen University Hospital
A group leader position at the Finsen Laboratory, Rigshospitalet, Copenhagen University Hospital is open for appointment as soon as possible. The position is av
candidate.hr-manager.net
June 3, 2025 at 1:38 PM
🎯 A tenure-track group leader position in translational cancer research is now open at Finsen Laboratory.
Come join an excellent and friendly research environment combining both the Rigshospitalet and the KU @ucph.bsky.social affiliated groups
candidate.hr-manager.net/ApplicationI...
Come join an excellent and friendly research environment combining both the Rigshospitalet and the KU @ucph.bsky.social affiliated groups
candidate.hr-manager.net/ApplicationI...
Chromosome Biology Club is back today!
Join us for two exciting talks:
🧬 Christian Nielsen (CCS/ICMM) on the distinct roles of condensins I & II in mitotic chromosome mechanics
🧬 Judit Pilmark (BRIC) on mutational signatures linked to NER deficiency
Starting at 4.45 pm
Followed by pizzas and beer
Join us for two exciting talks:
🧬 Christian Nielsen (CCS/ICMM) on the distinct roles of condensins I & II in mitotic chromosome mechanics
🧬 Judit Pilmark (BRIC) on mutational signatures linked to NER deficiency
Starting at 4.45 pm
Followed by pizzas and beer
May 27, 2025 at 10:10 AM
Chromosome Biology Club is back today!
Join us for two exciting talks:
🧬 Christian Nielsen (CCS/ICMM) on the distinct roles of condensins I & II in mitotic chromosome mechanics
🧬 Judit Pilmark (BRIC) on mutational signatures linked to NER deficiency
Starting at 4.45 pm
Followed by pizzas and beer
Join us for two exciting talks:
🧬 Christian Nielsen (CCS/ICMM) on the distinct roles of condensins I & II in mitotic chromosome mechanics
🧬 Judit Pilmark (BRIC) on mutational signatures linked to NER deficiency
Starting at 4.45 pm
Followed by pizzas and beer
Reposted by Duxin lab
Structural and functional insights into the interaction between Ku70/80 and Pol X family polymerases in NHEJ @natcomms.nature.com BRCT domain in recruitment and activity of two Pol X members in NHEJ: a unified model for the interaction of Pol X with Ku70/80.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Structural and functional insights into the interaction between Ku70/80 and Pol X family polymerases in NHEJ - Nature Communications
The molecular basis for the enrollment of X family DNA polymerases in non-homologous end joining (NHEJ) is unclear. Here the authors elucidate the structure of Pol λ within the DNA-PK long-range compl...
www.nature.com
May 13, 2025 at 3:04 PM
Structural and functional insights into the interaction between Ku70/80 and Pol X family polymerases in NHEJ @natcomms.nature.com BRCT domain in recruitment and activity of two Pol X members in NHEJ: a unified model for the interaction of Pol X with Ku70/80.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Duxin lab
The FEBS | EMBO Women in Science Award aims to highlight the major contributions made by female scientists to #LifeSciences research. Winners of the award are inspiring role models for future generations of #WomenInScience
Nominate a scientist by 15 May: www.embo.org/the-embo-com... 🧪
Nominate a scientist by 15 May: www.embo.org/the-embo-com... 🧪
FEBS|EMBO Women in Science Awardees – Nominate for the Women in Science Award – EMBO
Nominees should be female scientists working in an EMBC Member State or FEBS member country, who have made outstanding contributions to life sciences research and significantly advanced our…
www.embo.org
April 30, 2025 at 9:52 AM
The FEBS | EMBO Women in Science Award aims to highlight the major contributions made by female scientists to #LifeSciences research. Winners of the award are inspiring role models for future generations of #WomenInScience
Nominate a scientist by 15 May: www.embo.org/the-embo-com... 🧪
Nominate a scientist by 15 May: www.embo.org/the-embo-com... 🧪
Reposted by Duxin lab
New preprint from the lab @kaelamakins.bsky.social Genetic interactions between 53BP1-RIF1 and DNA-PKcs for DSB repair outcomes. www.biorxiv.org/content/10.1...
53BP1/RIF1 and DNA-PKcs show distinct genetic interactions with diverse chromosomal break repair outcomes.
DNA double strand breaks (DSBs) are the effective lesion of cancer radiotherapy and induce gene editing. 53BP1 accumulates at DSBs and is implicated in end joining (EJ) repair, but its influence on DS...
www.biorxiv.org
May 12, 2025 at 6:53 PM
New preprint from the lab @kaelamakins.bsky.social Genetic interactions between 53BP1-RIF1 and DNA-PKcs for DSB repair outcomes. www.biorxiv.org/content/10.1...
Reposted by Duxin lab
Our study highlights RNA as a direct template for DNA repair, revealing RT-DSBR as a previously unrecognized and potentially mutagenic pathway. Huge thanks to the incredible team who made this work possible! (5/5)
🔗 www.nature.com/articles/s41...
#GenomeInstability #DNARepair #RNA #CRISPR #DSB
🔗 www.nature.com/articles/s41...
#GenomeInstability #DNARepair #RNA #CRISPR #DSB
RNA transcripts serve as a template for double-strand break repair in human cells - Nature Communications
DNA double-stranded breaks threaten genome stability. Here, the authors show that transcript RNA serves as a repair template in human cells and identify Polζ as a key factor in RNA-templated DSB repai...
www.nature.com
May 12, 2025 at 5:49 PM
Our study highlights RNA as a direct template for DNA repair, revealing RT-DSBR as a previously unrecognized and potentially mutagenic pathway. Huge thanks to the incredible team who made this work possible! (5/5)
🔗 www.nature.com/articles/s41...
#GenomeInstability #DNARepair #RNA #CRISPR #DSB
🔗 www.nature.com/articles/s41...
#GenomeInstability #DNARepair #RNA #CRISPR #DSB
Reposted by Duxin lab
Today we celebrated #EuropeDay2025 showing the work we do at the RepliFate Doctoral Network in the public library Eugenio Trías @cbm-csic-uam.bsky.social @genomefun.bsky.social
May 8, 2025 at 8:41 PM
Today we celebrated #EuropeDay2025 showing the work we do at the RepliFate Doctoral Network in the public library Eugenio Trías @cbm-csic-uam.bsky.social @genomefun.bsky.social
Reposted by Duxin lab
🧬 New research discovers sequential phosphorylation of MRE11 by ATM and ATR prevents extensive DNA degradation at stalled replication forks
www.pnas.org/doi/abs/10.1...
www.pnas.org/doi/abs/10.1...
ATM priming and end resection–coupled phosphorylation of MRE11 is important for fork protection and replication restart | PNAS
The MRE11/RAD50/NBS1 (MRN) complex plays multiple roles in the maintenance of genome
stability. MRN is associated with replication forks to preserv...
www.pnas.org
April 29, 2025 at 4:12 AM
🧬 New research discovers sequential phosphorylation of MRE11 by ATM and ATR prevents extensive DNA degradation at stalled replication forks
www.pnas.org/doi/abs/10.1...
www.pnas.org/doi/abs/10.1...
🌍 #ClimateActionDay is here!
For the first time, the University of Copenhagen @ucph.bsky.social is joining forces to take real steps toward a greener future
At BRIC @bric-ucph.bsky.social, we’re putting words into action!
Small changes can make a big impact 🌱
#GreenLabs #SustainableScience
For the first time, the University of Copenhagen @ucph.bsky.social is joining forces to take real steps toward a greener future
At BRIC @bric-ucph.bsky.social, we’re putting words into action!
Small changes can make a big impact 🌱
#GreenLabs #SustainableScience
April 24, 2025 at 6:59 AM
🌍 #ClimateActionDay is here!
For the first time, the University of Copenhagen @ucph.bsky.social is joining forces to take real steps toward a greener future
At BRIC @bric-ucph.bsky.social, we’re putting words into action!
Small changes can make a big impact 🌱
#GreenLabs #SustainableScience
For the first time, the University of Copenhagen @ucph.bsky.social is joining forces to take real steps toward a greener future
At BRIC @bric-ucph.bsky.social, we’re putting words into action!
Small changes can make a big impact 🌱
#GreenLabs #SustainableScience
