@cperez-gonzalez.bsky.social
Pinned
1/ 🎉Excited to share our new preprint @vignjeviclab.bsky.social @davidbrueckner.bsky.social :
"Self-organization of tumor heterogeneity and plasticity"
biorxiv.org/content/10.1...
Tumor heterogeneity and plasticity drive metastasis and relapse. How is tumor patterning coordinated?
A thread👇
"Self-organization of tumor heterogeneity and plasticity"
biorxiv.org/content/10.1...
Tumor heterogeneity and plasticity drive metastasis and relapse. How is tumor patterning coordinated?
A thread👇
Reposted
A bit delayed, but excited to still share our latest paper, showing that intercellular forces transduced by E-cadherin activate EGFR-ERK signaling in epithelia by inducing EGFR ligand shedding! Mechanical and biochemical signals can act together within a single, linear cascade! tinyurl.com/mr9mj9j2
E-cadherin mechanotransduction activates EGFR-ERK signaling in epithelial monolayers by inducing ADAM-mediated ligand shedding
Epithelial stretching promotes the release of EGF receptor ligands that stimulate ERK activation.
tinyurl.com
September 13, 2025 at 6:05 PM
A bit delayed, but excited to still share our latest paper, showing that intercellular forces transduced by E-cadherin activate EGFR-ERK signaling in epithelia by inducing EGFR ligand shedding! Mechanical and biochemical signals can act together within a single, linear cascade! tinyurl.com/mr9mj9j2
Reposted
Paper alert! β-catenin forms condensates that help build cell–cell adhesions - showing its role goes beyond simply linking E-cadherin to α-cat/actin. Great collaboration with the Schuijers lab!
👉 nature.com/articles/s41...
👉 nature.com/articles/s41...
December 16, 2025 at 9:25 AM
Paper alert! β-catenin forms condensates that help build cell–cell adhesions - showing its role goes beyond simply linking E-cadherin to α-cat/actin. Great collaboration with the Schuijers lab!
👉 nature.com/articles/s41...
👉 nature.com/articles/s41...
Reposted
I am putting together a collection of video-protocols we (will) use in tissue mechanics research🌀
I hope to help consolidate established methods, but also showcase a few up-and-coming ones in our blooming field.
Abstract submission by March 2026! Get in touch✨
#JoVETissueMech
#ReproducibleTissueMech
I hope to help consolidate established methods, but also showcase a few up-and-coming ones in our blooming field.
Abstract submission by March 2026! Get in touch✨
#JoVETissueMech
#ReproducibleTissueMech
December 16, 2025 at 2:28 PM
I am putting together a collection of video-protocols we (will) use in tissue mechanics research🌀
I hope to help consolidate established methods, but also showcase a few up-and-coming ones in our blooming field.
Abstract submission by March 2026! Get in touch✨
#JoVETissueMech
#ReproducibleTissueMech
I hope to help consolidate established methods, but also showcase a few up-and-coming ones in our blooming field.
Abstract submission by March 2026! Get in touch✨
#JoVETissueMech
#ReproducibleTissueMech
Reposted
Want to image cells protecting the embryo during early development? We're hiring! 1 Postdoc, 1 PhD student, and 1 Research Technician. Come to Barcelona! www.embryobioimaging.com
December 9, 2025 at 5:44 PM
Want to image cells protecting the embryo during early development? We're hiring! 1 Postdoc, 1 PhD student, and 1 Research Technician. Come to Barcelona! www.embryobioimaging.com
Reposted
It’s time to talk about epithelial geometry and cancer. How can the architecture of an epithelium affect how tumors will grow and spread? In this thread, @jorgealmagro.bsky.social
November 23, 2025 at 8:01 AM
It’s time to talk about epithelial geometry and cancer. How can the architecture of an epithelium affect how tumors will grow and spread? In this thread, @jorgealmagro.bsky.social
Thanks Ronja!! Sorry I missed your message! 🫣🫣
November 10, 2025 at 7:04 AM
Thanks Ronja!! Sorry I missed your message! 🫣🫣
Reposted
We finally made it over here ! And excited to share our new paper on mechanical forces regulating stem cell plasticity in colorectal cancer, involving force transduction via mechanosensitive calcium channels! www.cell.com/cell-reports...
Mechanosensitive calcium channels and integrins coordinate the reprogramming of colorectal cancer cells into a fetal-like state
van der Net et al. show that mechanical interactions with the stromal component collagen
I trigger reprogramming of colorectal cancer cells into a fetal-like state, through
mechanosensitive integrins ...
www.cell.com
November 7, 2025 at 4:33 PM
We finally made it over here ! And excited to share our new paper on mechanical forces regulating stem cell plasticity in colorectal cancer, involving force transduction via mechanosensitive calcium channels! www.cell.com/cell-reports...
Reposted
Really excited to present the results of a fantastic collaboration with Jesse Veenvliet @jesseveenvliet.bsky.social @mpi-cbg.de @poldresden.bsky.social 🤩
We find a unique mechanism for body axis elongation in mammals, different from other vertebrate species
➡️ www.biorxiv.org/content/10.1...
We find a unique mechanism for body axis elongation in mammals, different from other vertebrate species
➡️ www.biorxiv.org/content/10.1...
October 28, 2025 at 8:25 AM
Really excited to present the results of a fantastic collaboration with Jesse Veenvliet @jesseveenvliet.bsky.social @mpi-cbg.de @poldresden.bsky.social 🤩
We find a unique mechanism for body axis elongation in mammals, different from other vertebrate species
➡️ www.biorxiv.org/content/10.1...
We find a unique mechanism for body axis elongation in mammals, different from other vertebrate species
➡️ www.biorxiv.org/content/10.1...
Reposted
➡️ This important video was missing from yesterday’s #Bluetorial.
CRC organoids under treatment with KRAS inhibitor RMC-9945, rapidly acquire a LGR5+ cancer stem cell-like phenotype, allowing them to bypass KRAS inhibition, and eventually resume growth.
Centonze et al., in #CancerDiscovery
#AACR
CRC organoids under treatment with KRAS inhibitor RMC-9945, rapidly acquire a LGR5+ cancer stem cell-like phenotype, allowing them to bypass KRAS inhibition, and eventually resume growth.
Centonze et al., in #CancerDiscovery
#AACR
October 24, 2025 at 6:47 AM
➡️ This important video was missing from yesterday’s #Bluetorial.
CRC organoids under treatment with KRAS inhibitor RMC-9945, rapidly acquire a LGR5+ cancer stem cell-like phenotype, allowing them to bypass KRAS inhibition, and eventually resume growth.
Centonze et al., in #CancerDiscovery
#AACR
CRC organoids under treatment with KRAS inhibitor RMC-9945, rapidly acquire a LGR5+ cancer stem cell-like phenotype, allowing them to bypass KRAS inhibition, and eventually resume growth.
Centonze et al., in #CancerDiscovery
#AACR
Reposted
📣Announcing the 4th edition of the EMBL‑IBEC Conference on “Engineering Multicellular Systems”, taking place 11–13 March 2026 in Barcelona. Exploring organoids, mechanobiology, embryo models, organ-on-chip systems, multiomics and more. Abstracts open now!
events.ibecbarcelona.eu/embl-ibec-co...
events.ibecbarcelona.eu/embl-ibec-co...
October 22, 2025 at 2:06 PM
📣Announcing the 4th edition of the EMBL‑IBEC Conference on “Engineering Multicellular Systems”, taking place 11–13 March 2026 in Barcelona. Exploring organoids, mechanobiology, embryo models, organ-on-chip systems, multiomics and more. Abstracts open now!
events.ibecbarcelona.eu/embl-ibec-co...
events.ibecbarcelona.eu/embl-ibec-co...
Thanks Mathieu. Indeed, in free growth conditions stem cells also appear at the center (as in /3). It just takes longer to reach such levels of compression.
October 22, 2025 at 12:56 PM
Thanks Mathieu. Indeed, in free growth conditions stem cells also appear at the center (as in /3). It just takes longer to reach such levels of compression.
Reposted
Pathologists have used nuclear & tissue shapes to diagnose #cancer for decades, but what is the molecular basis for this? In our latest work, we develop a computational pipeline to figure this out in #colorectalcancer! Check out 🧵& preprint #mechanobiology #stemcells www.biorxiv.org/content/10.1...
August 15, 2025 at 10:50 AM
Pathologists have used nuclear & tissue shapes to diagnose #cancer for decades, but what is the molecular basis for this? In our latest work, we develop a computational pipeline to figure this out in #colorectalcancer! Check out 🧵& preprint #mechanobiology #stemcells www.biorxiv.org/content/10.1...
Reposted
On cell extrusion in the #intestine!
After 80 years of observations, we finally took a deeper look thanks to 2D #organoids. We report:
3D #forces, #extrusion still only in the villus even without curvature, #lamellipodia generate 3D force.. and more. Have a look!
www.biorxiv.org/content/10.1...
After 80 years of observations, we finally took a deeper look thanks to 2D #organoids. We report:
3D #forces, #extrusion still only in the villus even without curvature, #lamellipodia generate 3D force.. and more. Have a look!
www.biorxiv.org/content/10.1...
July 4, 2025 at 10:21 AM
On cell extrusion in the #intestine!
After 80 years of observations, we finally took a deeper look thanks to 2D #organoids. We report:
3D #forces, #extrusion still only in the villus even without curvature, #lamellipodia generate 3D force.. and more. Have a look!
www.biorxiv.org/content/10.1...
After 80 years of observations, we finally took a deeper look thanks to 2D #organoids. We report:
3D #forces, #extrusion still only in the villus even without curvature, #lamellipodia generate 3D force.. and more. Have a look!
www.biorxiv.org/content/10.1...
Reposted
New job, new preprint! We found that embryo implantation can be understood as active wetting! Embryos from older mothers have trouble implanting because they are too contractile and viscous. Check out the wonderful thread (and movies!) by @katecavanaugh.bsky.social.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
October 2, 2025 at 8:04 AM
New job, new preprint! We found that embryo implantation can be understood as active wetting! Embryos from older mothers have trouble implanting because they are too contractile and viscous. Check out the wonderful thread (and movies!) by @katecavanaugh.bsky.social.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Thanks, Raimon! Yes, first... "tweet"? How do you call it here?
October 17, 2025 at 2:47 PM
Thanks, Raimon! Yes, first... "tweet"? How do you call it here?
Gràcies, Ricard!!
October 17, 2025 at 9:56 AM
Gràcies, Ricard!!
Thank you!!! 😊😊
October 17, 2025 at 7:51 AM
Thank you!!! 😊😊
A special mention to Jochen Guck. My deepest condolences to his group and loved ones. This is just one more example of his generosity and immense contributions to science.🌹
October 16, 2025 at 9:55 PM
A special mention to Jochen Guck. My deepest condolences to his group and loved ones. This is just one more example of his generosity and immense contributions to science.🌹
10/ This was a huge collaborative work hand in hand with
@davidbrueckner.bsky.social , together with @vignjeviclab.bsky.social, @ehannezo.bsky.social .bsky.social , Julie Guillermet's lab, Jochen Guck's lab, @vanrheenenlab.bsky.social and Martijn's Gloerich lab. Thank you everybody!!! 🙏🙏🙏🙏
@davidbrueckner.bsky.social , together with @vignjeviclab.bsky.social, @ehannezo.bsky.social .bsky.social , Julie Guillermet's lab, Jochen Guck's lab, @vanrheenenlab.bsky.social and Martijn's Gloerich lab. Thank you everybody!!! 🙏🙏🙏🙏
ky.social
October 16, 2025 at 9:55 PM
10/ This was a huge collaborative work hand in hand with
@davidbrueckner.bsky.social , together with @vignjeviclab.bsky.social, @ehannezo.bsky.social .bsky.social , Julie Guillermet's lab, Jochen Guck's lab, @vanrheenenlab.bsky.social and Martijn's Gloerich lab. Thank you everybody!!! 🙏🙏🙏🙏
@davidbrueckner.bsky.social , together with @vignjeviclab.bsky.social, @ehannezo.bsky.social .bsky.social , Julie Guillermet's lab, Jochen Guck's lab, @vanrheenenlab.bsky.social and Martijn's Gloerich lab. Thank you everybody!!! 🙏🙏🙏🙏
9/ Conclusion: Tumors follow reproducible self-organization programs that are sufficient to pattern cell states in time and space.
We propose that such tissue-intrinsic programs cooperate with microenvironmental cues to collectively drive phenotypic heterogeneity and plasticity.
We propose that such tissue-intrinsic programs cooperate with microenvironmental cues to collectively drive phenotypic heterogeneity and plasticity.
October 16, 2025 at 9:55 PM
9/ Conclusion: Tumors follow reproducible self-organization programs that are sufficient to pattern cell states in time and space.
We propose that such tissue-intrinsic programs cooperate with microenvironmental cues to collectively drive phenotypic heterogeneity and plasticity.
We propose that such tissue-intrinsic programs cooperate with microenvironmental cues to collectively drive phenotypic heterogeneity and plasticity.
8/ scRNA sequencing, imaging and theory showed that large organoid monolayers exhibit a biosynthetic arrest at the center, followed by a loss of stemness and death (A pattern observed in vivo by
@batllelab.bsky.social
linkinghub.elsevier.com/retrieve/pii... )
@batllelab.bsky.social
linkinghub.elsevier.com/retrieve/pii... )
October 16, 2025 at 9:55 PM
8/ scRNA sequencing, imaging and theory showed that large organoid monolayers exhibit a biosynthetic arrest at the center, followed by a loss of stemness and death (A pattern observed in vivo by
@batllelab.bsky.social
linkinghub.elsevier.com/retrieve/pii... )
@batllelab.bsky.social
linkinghub.elsevier.com/retrieve/pii... )
7/ Surprisingly, hierarchy also emerges in vitro above a critical size defined by the intrinsic characteristic length of the stem cell compartment.
Below critical size ➡️ homogeneous proliferation
Above critical size ➡️ hierarchy
Below critical size ➡️ homogeneous proliferation
Above critical size ➡️ hierarchy
October 16, 2025 at 9:55 PM
7/ Surprisingly, hierarchy also emerges in vitro above a critical size defined by the intrinsic characteristic length of the stem cell compartment.
Below critical size ➡️ homogeneous proliferation
Above critical size ➡️ hierarchy
Below critical size ➡️ homogeneous proliferation
Above critical size ➡️ hierarchy
6/ But tumor patterning is more complex than just stem- and fetal-like states...
Above a critical tumor size, proliferation and death become spatially compartmentalized: cells divide at the edge and die at the core — a pattern typically attributed to hypoxia.
Above a critical tumor size, proliferation and death become spatially compartmentalized: cells divide at the edge and die at the core — a pattern typically attributed to hypoxia.
October 16, 2025 at 9:55 PM
6/ But tumor patterning is more complex than just stem- and fetal-like states...
Above a critical tumor size, proliferation and death become spatially compartmentalized: cells divide at the edge and die at the core — a pattern typically attributed to hypoxia.
Above a critical tumor size, proliferation and death become spatially compartmentalized: cells divide at the edge and die at the core — a pattern typically attributed to hypoxia.
5/ Indeed!
Stress measurements (in vivo & in vitro) show a correlation between compression and Lgr5. With
Jochen's Guck Lab
.
To test causality, we physically confined organoids and tumors — and confinement alone was sufficient to boost stemness! With
Julie Guillermet Lab
Stress measurements (in vivo & in vitro) show a correlation between compression and Lgr5. With
Jochen's Guck Lab
.
To test causality, we physically confined organoids and tumors — and confinement alone was sufficient to boost stemness! With
Julie Guillermet Lab
October 16, 2025 at 9:55 PM
5/ Indeed!
Stress measurements (in vivo & in vitro) show a correlation between compression and Lgr5. With
Jochen's Guck Lab
.
To test causality, we physically confined organoids and tumors — and confinement alone was sufficient to boost stemness! With
Julie Guillermet Lab
Stress measurements (in vivo & in vitro) show a correlation between compression and Lgr5. With
Jochen's Guck Lab
.
To test causality, we physically confined organoids and tumors — and confinement alone was sufficient to boost stemness! With
Julie Guillermet Lab
4/ The interplay between proliferation and expansion generates cell density gradients from tumor edge to core. Interestingly, stem cells appear at high densities and fetal-like cells at low densities.
Could emergent mechanical gradients coordinate tumor cell state transitions?🤔
Could emergent mechanical gradients coordinate tumor cell state transitions?🤔
October 16, 2025 at 9:55 PM
4/ The interplay between proliferation and expansion generates cell density gradients from tumor edge to core. Interestingly, stem cells appear at high densities and fetal-like cells at low densities.
Could emergent mechanical gradients coordinate tumor cell state transitions?🤔
Could emergent mechanical gradients coordinate tumor cell state transitions?🤔