Charlotte Fare
@charlottefare.bsky.social
Principal Scientist at GSK • Still learning
Previously: JHU, UPenn, UNC-CH
🧬🦠🔬🧠🐈🌱
Previously: JHU, UPenn, UNC-CH
🧬🦠🔬🧠🐈🌱
Large-scale drug screening in iPSC-derived motor neurons from sporadic ALS patients identifies a potential combinatorial therapy www.nature.com/articles/s41...
Large-scale drug screening in iPSC-derived motor neurons from sporadic ALS patients identifies a potential combinatorial therapy - Nature Neuroscience
In this study, the authors generated iPSC lines from more than 100 sporadic ALS cases, which recapitulated key disease phenotypes and enabled large-scale drug screening, identifying a promising combin...
www.nature.com
November 24, 2025 at 7:07 PM
Large-scale drug screening in iPSC-derived motor neurons from sporadic ALS patients identifies a potential combinatorial therapy www.nature.com/articles/s41...
Fulfilling Multiple Roles in PROTAC Design: The Emerging Potential of Oligonucleotides doi.org/10.26434/che...
November 24, 2025 at 3:35 PM
Fulfilling Multiple Roles in PROTAC Design: The Emerging Potential of Oligonucleotides doi.org/10.26434/che...
FUS nanoclusters are a distinct state within the dilute phase rdcu.be/eQkxU
FUS nanoclusters are a distinct state within the dilute phase
Nature Communications - FUS drives the formation of biomolecular condensates in cells. Here, the authors reveal the dynamics and biophysical properties of FUS nanoclusters, an intermediate state in...
rdcu.be
November 17, 2025 at 12:04 PM
FUS nanoclusters are a distinct state within the dilute phase rdcu.be/eQkxU
Reposted by Charlotte Fare
www.biorxiv.org/content/10.1... @rohitpappu68.bsky.social and Jim Shorter latest banger just dropped
Nuclear-import receptors remodel the dilute phase to suppress phase transitions of RNA-binding proteins with prion-like domains.
RNA-binding proteins (RBPs) with prion-like domains, including FUS, hnRNPA1, and hnRNPA2, assemble into functional, metastable condensates that organize ribostasis, but can also transition into self-t...
www.biorxiv.org
November 16, 2025 at 2:11 AM
www.biorxiv.org/content/10.1... @rohitpappu68.bsky.social and Jim Shorter latest banger just dropped
Emergent Chemical Reactivity and Complexity of RNA Condensates onlinelibrary.wiley.com/doi/10.1002/...
Emergent Chemical Reactivity and Complexity of RNA Condensates
RNA condensates capable of substrate enrichment and catalysis modulate their environment. Could this start a catalytic selection loop or evolution?
onlinelibrary.wiley.com
November 14, 2025 at 12:59 PM
Emergent Chemical Reactivity and Complexity of RNA Condensates onlinelibrary.wiley.com/doi/10.1002/...
Reposted by Charlotte Fare
Stress granules and protein aggregates reveal intracellular resource competition https://www.biorxiv.org/content/10.1101/2025.11.08.687377v1
November 10, 2025 at 8:30 PM
Stress granules and protein aggregates reveal intracellular resource competition https://www.biorxiv.org/content/10.1101/2025.11.08.687377v1
Reposted by Charlotte Fare
I found Squeaky & Albert six years ago today, on Halloween eve. What unexpected joy they have brought…
October 31, 2025 at 4:48 PM
I found Squeaky & Albert six years ago today, on Halloween eve. What unexpected joy they have brought…
RNA-Mediated Inhibition Mechanism of Liquid–Liquid Phase Separation and Subsequent Aggregation Revealed by Raman Microscopy pubs.acs.org/doi/full/10....
RNA-Mediated Inhibition Mechanism of Liquid–Liquid Phase Separation and Subsequent Aggregation Revealed by Raman Microscopy
Liquid–liquid phase separation (LLPS) is a phenomenon where homogeneous solutions of biomacromolecules separate into two liquid phases and generate liquid droplets enriched in specific biomolecules. L...
pubs.acs.org
November 7, 2025 at 7:12 PM
RNA-Mediated Inhibition Mechanism of Liquid–Liquid Phase Separation and Subsequent Aggregation Revealed by Raman Microscopy pubs.acs.org/doi/full/10....
RNA G-Quadruplexes Function as a Tunable Switch of FUS Phase Separation www.biorxiv.org/content/10.1...
RNA G-Quadruplexes Function as a Tunable Switch of FUS Phase Separation
FUS undergoes liquid-liquid phase separation (LLPS) to support essential cellular functions, but aberrant phase transitions promote toxic aggregation in neurodegenerative disease. Short RNA oligonucle...
www.biorxiv.org
November 4, 2025 at 7:32 PM
RNA G-Quadruplexes Function as a Tunable Switch of FUS Phase Separation www.biorxiv.org/content/10.1...
Reposted by Charlotte Fare
TDP-43 skein-like inclusions are formed by BAG3- and HSP70-guided co-aggregation with actin-binding proteins
www.nature.com/articles/s41...
www.nature.com/articles/s41...
TDP-43 skein-like inclusions are formed by BAG3- and HSP70-guided co-aggregation with actin-binding proteins - Nature Cell Biology
Lu et al. show that, under proteotoxic stress, TDP-43 inclusions of skein-like morphology are guided by the chaperone HSP70 and its nucleotide exchange factor BAG3 to induce TDP-43 co-aggregation with...
www.nature.com
October 31, 2025 at 3:06 PM
TDP-43 skein-like inclusions are formed by BAG3- and HSP70-guided co-aggregation with actin-binding proteins
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Charlotte Fare
SuB3 a Simple Subcellular Fractionation Protocol for Localization Studies of Nucleic Acids and Proteins https://www.biorxiv.org/content/10.1101/2025.10.21.683662v1
October 22, 2025 at 2:19 AM
SuB3 a Simple Subcellular Fractionation Protocol for Localization Studies of Nucleic Acids and Proteins https://www.biorxiv.org/content/10.1101/2025.10.21.683662v1
Improved targeted delivery of antisense oligonucleotide with an antibody mask academic.oup.com/nar/article/...
Improved targeted delivery of antisense oligonucleotide with an antibody mask
Abstract. Antisense oligonucleotides (ASOs) are synthetic nucleic acid strands designed to modulate gene expression by binding to RNA transcripts. In brain
academic.oup.com
November 2, 2025 at 7:08 PM
Improved targeted delivery of antisense oligonucleotide with an antibody mask academic.oup.com/nar/article/...
TDP-43-dependent mis-splicing of KCNQ2 triggers intrinsic neuronal hyperexcitability in ALS/FTD www.nature.com/articles/s41...
TDP-43-dependent mis-splicing of KCNQ2 triggers intrinsic neuronal hyperexcitability in ALS/FTD - Nature Neuroscience
TDP-43 dysfunction in ALS/FTD causes faulty splicing of the KCNQ2 ion channel, leading to toxic protein buildup, neuron hyperactivity and a potential new biomarker and treatment target using RNA-based...
www.nature.com
November 1, 2025 at 10:18 PM
TDP-43-dependent mis-splicing of KCNQ2 triggers intrinsic neuronal hyperexcitability in ALS/FTD www.nature.com/articles/s41...
Reposted by Charlotte Fare
New study by @noalipstein.bsky.social and Nils Brose, @mpi-nat.bsky.social links UNC13A gene variants to a new developmental disorder — offering insights into ALS and potential therapies. 👏
Read the full paper: www.nature.com/articles/s41...
Read the full paper: www.nature.com/articles/s41...
Pathogenic UNC13A variants cause a neurodevelopmental syndrome by impairing synaptic function - Nature Genetics
Pathogenic variants in UNC13A underlie a novel neurodevelopmental syndrome, with three subtypes defined by distinct genotypes with varying clinical and functional impacts characterized in cellular and...
www.nature.com
October 22, 2025 at 11:57 AM
New study by @noalipstein.bsky.social and Nils Brose, @mpi-nat.bsky.social links UNC13A gene variants to a new developmental disorder — offering insights into ALS and potential therapies. 👏
Read the full paper: www.nature.com/articles/s41...
Read the full paper: www.nature.com/articles/s41...
Reposted by Charlotte Fare
Conserved intrinsically disordered region of DNAJB6 dictates its surveillance of FG-Nup condensates
I've been lucky enough to work on this paper of Tessa and Maiara in Veenhoff and Kampinga's lab.
www.biorxiv.org/content/10.1...
I've been lucky enough to work on this paper of Tessa and Maiara in Veenhoff and Kampinga's lab.
www.biorxiv.org/content/10.1...
Conserved intrinsically disordered region of DNAJB6 dictates its surveillance of FG-Nup condensates
Molecular chaperones can prevent protein aggregation and assist proteins in reaching their structurally functional state. The molecular chaperone DNAJB6, a J-domain protein that partners with Hsp70s a...
www.biorxiv.org
October 21, 2025 at 5:54 AM
Conserved intrinsically disordered region of DNAJB6 dictates its surveillance of FG-Nup condensates
I've been lucky enough to work on this paper of Tessa and Maiara in Veenhoff and Kampinga's lab.
www.biorxiv.org/content/10.1...
I've been lucky enough to work on this paper of Tessa and Maiara in Veenhoff and Kampinga's lab.
www.biorxiv.org/content/10.1...
G4 Oligonucleotide-Based Chaperones of hnRNPA1 www.mdpi.com/3546964
www.mdpi.com
October 22, 2025 at 1:35 PM
G4 Oligonucleotide-Based Chaperones of hnRNPA1 www.mdpi.com/3546964
Cleavable Hydrophobic Anchors Optimize Immunomodulatory Effects of Oligonucleotides pubs.acs.org/doi/abs/10.1...
Cleavable Hydrophobic Anchors Optimize Immunomodulatory Effects of Oligonucleotides
Oligonucleotides (ODNs) are useful in a wide variety of biomedical applications, spanning diagnostics, and therapeutics but are susceptible to nuclease degradation and exhibit poor cellular uptake. St...
pubs.acs.org
October 17, 2025 at 11:56 AM
Cleavable Hydrophobic Anchors Optimize Immunomodulatory Effects of Oligonucleotides pubs.acs.org/doi/abs/10.1...
Synthetic chaperone based on Hsp90-Tau interaction inhibits Tau aggregation and rescues physiological Tau-Microtubule interaction www.nature.com/articles/s41...
Synthetic chaperone based on Hsp90-Tau interaction inhibits Tau aggregation and rescues physiological Tau-Microtubule interaction - Nature Communications
Tau aggregation, a hallmark of Alzheimer’s disease, disrupts neuron structure. Aging weakens chaperone defenses like Hsp90. This study designs β-Hsp90, a small peptide mimicking Hsp90, to prevent Tau aggregation, offering promise for new amyloid disease drugs.
www.nature.com
October 5, 2025 at 1:49 PM
Synthetic chaperone based on Hsp90-Tau interaction inhibits Tau aggregation and rescues physiological Tau-Microtubule interaction www.nature.com/articles/s41...
Reposted by Charlotte Fare
NEW: ProPublica obtained records from the Department of Agriculture that detail the millions of pounds of food, down to the number of eggs, that never reached food banks because of the Trump administration’s cuts to an aid program.
Trump Canceled 94 Million Pounds of Food Aid. Here’s What Never Arrived.
ProPublica obtained records from the Department of Agriculture that detail the millions of pounds of food, down to the number of eggs, that never reached food banks because of the administration’s cut...
projects.propublica.org
October 3, 2025 at 12:15 PM
NEW: ProPublica obtained records from the Department of Agriculture that detail the millions of pounds of food, down to the number of eggs, that never reached food banks because of the Trump administration’s cuts to an aid program.
Reposted by Charlotte Fare
Excited to share our first preprint! We developed an image-based pooled screen to uncover regulators of HP1 condensates and discovered a link with intronic RNA and RNA processing. 👏 Congrats to all authors, especially Matthew, Shaopu & Chris!
An image-based CRISPR screen reveals splicing-mediated control of HP1α condensates https://www.biorxiv.org/content/10.1101/2025.09.21.676939v1
September 22, 2025 at 7:05 PM
Excited to share our first preprint! We developed an image-based pooled screen to uncover regulators of HP1 condensates and discovered a link with intronic RNA and RNA processing. 👏 Congrats to all authors, especially Matthew, Shaopu & Chris!
Matrin-3 forms spherical and wormlike assemblies that are modulated by RNA binding and ALS/FTD-associated mutations www.cell.com/molecular-ce... @jackrellab.bsky.social @rohitpappu68.bsky.social
Matrin-3 forms spherical and wormlike assemblies that are modulated by RNA binding and ALS/FTD-associated mutations
Sprunger et al. have characterized the intrinsically disordered protein Matrin-3,
which is implicated in amyotrophic lateral sclerosis (ALS). They find that Matrin-3
forms unusual nanoscale spherical ...
www.cell.com
September 23, 2025 at 5:01 PM
Matrin-3 forms spherical and wormlike assemblies that are modulated by RNA binding and ALS/FTD-associated mutations www.cell.com/molecular-ce... @jackrellab.bsky.social @rohitpappu68.bsky.social
Prematurely Aged Human Microglia Exhibit Impaired Stress Response and Defective Nucleocytoplasmic Shuttling of ALS Associated FUS onlinelibrary.wiley.com/doi/10.1111/...
Prematurely Aged Human Microglia Exhibit Impaired Stress Response and Defective Nucleocytoplasmic Shuttling of ALS Associated FUS
We developed a human microglia aging model by doxycycline-induced GFP-progerin expression, which offers a valuable tool to study human microglial aging and its contribution to neurodegeneration. Indu...
onlinelibrary.wiley.com
September 23, 2025 at 4:53 PM
Prematurely Aged Human Microglia Exhibit Impaired Stress Response and Defective Nucleocytoplasmic Shuttling of ALS Associated FUS onlinelibrary.wiley.com/doi/10.1111/...
Reposted by Charlotte Fare
Small heat shock proteins HspB1 and HspB5 differentially alter the condensation and aggregation of the TDP-43 low complexity domain https://www.biorxiv.org/content/10.1101/2025.09.17.672772v1
September 18, 2025 at 4:46 PM
Small heat shock proteins HspB1 and HspB5 differentially alter the condensation and aggregation of the TDP-43 low complexity domain https://www.biorxiv.org/content/10.1101/2025.09.17.672772v1
Reposted by Charlotte Fare
A new paper reports on drug design principles to adjust for targets that partition into biomolecular condensates by considering condensate micropolarity
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Navigating condensate micropolarity to enhance small-molecule drug targeting - Nature Chemical Biology
Using computational and experimental methods, Ouyang and Chen et al. reveal a drug design principle that the hydrophobic properties of small-molecule drugs significantly influence their ability to eng...
www.nature.com
September 15, 2025 at 7:35 PM
A new paper reports on drug design principles to adjust for targets that partition into biomolecular condensates by considering condensate micropolarity
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Antisense oligonucleotide depletion of CCDC146 is a broad-spectrum therapeutic strategy for ALS www.medrxiv.org/content/10.1...
Antisense oligonucleotide depletion of CCDC146 is a broad-spectrum therapeutic strategy for ALS
Amyotrophic lateral sclerosis (ALS) is a heritable and incurable disease defined by the degeneration of motor neurons (MNs), yet the genetics of ALS remain partially understood. Using a genomic deep l...
www.medrxiv.org
September 15, 2025 at 11:46 AM
Antisense oligonucleotide depletion of CCDC146 is a broad-spectrum therapeutic strategy for ALS www.medrxiv.org/content/10.1...