Christopher Frøhlich
@cfr016.bsky.social
Enzyme evolutionist at UiT- The Arctic University of Norwary
Pinned
Evolution tunes functional sub-state interconversion to boost enzyme function
Enzymes do not operate as static structures, but continuously fluctuate between different conformations. Enzymes therefore dynamically sample conformations with varying catalytic activity. However, it...
www.biorxiv.org
🚨PREPRINT🚨
Enzyme function does not rely only on static structure 🧩, but also on the rate at which functional sub-states interconvert ⏱️, making this interconversion subject to natural selection 🧬.
#OXA-48 #ceftazidime #enzymeevolution
www.biorxiv.org/content/10.6...
Enzyme function does not rely only on static structure 🧩, but also on the rate at which functional sub-states interconvert ⏱️, making this interconversion subject to natural selection 🧬.
#OXA-48 #ceftazidime #enzymeevolution
www.biorxiv.org/content/10.6...
🚨PREPRINT🚨
Enzyme function does not rely only on static structure 🧩, but also on the rate at which functional sub-states interconvert ⏱️, making this interconversion subject to natural selection 🧬.
#OXA-48 #ceftazidime #enzymeevolution
www.biorxiv.org/content/10.6...
Enzyme function does not rely only on static structure 🧩, but also on the rate at which functional sub-states interconvert ⏱️, making this interconversion subject to natural selection 🧬.
#OXA-48 #ceftazidime #enzymeevolution
www.biorxiv.org/content/10.6...
Evolution tunes functional sub-state interconversion to boost enzyme function
Enzymes do not operate as static structures, but continuously fluctuate between different conformations. Enzymes therefore dynamically sample conformations with varying catalytic activity. However, it...
www.biorxiv.org
January 1, 2026 at 10:26 AM
🚨PREPRINT🚨
Enzyme function does not rely only on static structure 🧩, but also on the rate at which functional sub-states interconvert ⏱️, making this interconversion subject to natural selection 🧬.
#OXA-48 #ceftazidime #enzymeevolution
www.biorxiv.org/content/10.6...
Enzyme function does not rely only on static structure 🧩, but also on the rate at which functional sub-states interconvert ⏱️, making this interconversion subject to natural selection 🧬.
#OXA-48 #ceftazidime #enzymeevolution
www.biorxiv.org/content/10.6...
Living in the Arctic 🥶 means Christmas comes early 🎁🥳
10 MNOK (≈1 MUSD) Early Career Investigator grant from the Research Council of Norway to work with the fabulous @bunzela.bsky.social (Max Planck Institute) on uncovering the molecular mechanisms of antibiotic resistance.
#AMR #beta-lactamases
10 MNOK (≈1 MUSD) Early Career Investigator grant from the Research Council of Norway to work with the fabulous @bunzela.bsky.social (Max Planck Institute) on uncovering the molecular mechanisms of antibiotic resistance.
#AMR #beta-lactamases
December 15, 2025 at 3:56 PM
Living in the Arctic 🥶 means Christmas comes early 🎁🥳
10 MNOK (≈1 MUSD) Early Career Investigator grant from the Research Council of Norway to work with the fabulous @bunzela.bsky.social (Max Planck Institute) on uncovering the molecular mechanisms of antibiotic resistance.
#AMR #beta-lactamases
10 MNOK (≈1 MUSD) Early Career Investigator grant from the Research Council of Norway to work with the fabulous @bunzela.bsky.social (Max Planck Institute) on uncovering the molecular mechanisms of antibiotic resistance.
#AMR #beta-lactamases
Interested in #β-lactamase inhibitors? 🧪
Our new #mSphere paper shows how a covalent boronic-acid + chelator fusion ⚗️ can restore β-lactam susceptibility in metallo-β-lactamase producers, even in variants that are difficult to inhibit with #taniborbactam.
journals.asm.org/doi/full/10....
Our new #mSphere paper shows how a covalent boronic-acid + chelator fusion ⚗️ can restore β-lactam susceptibility in metallo-β-lactamase producers, even in variants that are difficult to inhibit with #taniborbactam.
journals.asm.org/doi/full/10....
APC24-7, a covalent combination of boronic acid and chelator moieties, restores β-lactam efficiency against metallo-β-lactamase-producers | mSphere
The ability of bacteria such as Klebsiella pneumoniae and Escherichia coli to circumvent antimicrobial chemotherapy has become a global public health crisis.
The high prevalence of β-lactamase enzymes...
journals.asm.org
December 4, 2025 at 3:52 PM
Interested in #β-lactamase inhibitors? 🧪
Our new #mSphere paper shows how a covalent boronic-acid + chelator fusion ⚗️ can restore β-lactam susceptibility in metallo-β-lactamase producers, even in variants that are difficult to inhibit with #taniborbactam.
journals.asm.org/doi/full/10....
Our new #mSphere paper shows how a covalent boronic-acid + chelator fusion ⚗️ can restore β-lactam susceptibility in metallo-β-lactamase producers, even in variants that are difficult to inhibit with #taniborbactam.
journals.asm.org/doi/full/10....
Is it too early for a year review? 🤔
Research stays at ETH Zürich with Adrian Bunzel and at UBC Vancouver with Nobu 🤓.
Now I’m moving labs to start as Associate Professor at UiT, which means organising freezer contents 📦.
Two β-lactamase evolution preprints coming soon 🧬.
Stay tuned!
Research stays at ETH Zürich with Adrian Bunzel and at UBC Vancouver with Nobu 🤓.
Now I’m moving labs to start as Associate Professor at UiT, which means organising freezer contents 📦.
Two β-lactamase evolution preprints coming soon 🧬.
Stay tuned!
November 22, 2025 at 3:18 PM
Is it too early for a year review? 🤔
Research stays at ETH Zürich with Adrian Bunzel and at UBC Vancouver with Nobu 🤓.
Now I’m moving labs to start as Associate Professor at UiT, which means organising freezer contents 📦.
Two β-lactamase evolution preprints coming soon 🧬.
Stay tuned!
Research stays at ETH Zürich with Adrian Bunzel and at UBC Vancouver with Nobu 🤓.
Now I’m moving labs to start as Associate Professor at UiT, which means organising freezer contents 📦.
Two β-lactamase evolution preprints coming soon 🧬.
Stay tuned!
🔄 #Enzymes can adopt multiple #conformations.
⚡ Some of these act as functional sub-states.
📚 We summarised recent studies, the methods used to uncover these hidden states, and the challenges ahead in linking them to #proteinevolution.
www.sciencedirect.com/science/arti...
⚡ Some of these act as functional sub-states.
📚 We summarised recent studies, the methods used to uncover these hidden states, and the challenges ahead in linking them to #proteinevolution.
www.sciencedirect.com/science/arti...
Functional sub-states link conformational landscapes and protein evolution
The intrinsic conformational flexibility of proteins creates structural heterogeneity, giving rise to conformational ensembles within the energy lands…
www.sciencedirect.com
August 17, 2025 at 10:46 AM
🔄 #Enzymes can adopt multiple #conformations.
⚡ Some of these act as functional sub-states.
📚 We summarised recent studies, the methods used to uncover these hidden states, and the challenges ahead in linking them to #proteinevolution.
www.sciencedirect.com/science/arti...
⚡ Some of these act as functional sub-states.
📚 We summarised recent studies, the methods used to uncover these hidden states, and the challenges ahead in linking them to #proteinevolution.
www.sciencedirect.com/science/arti...
Reposted by Christopher Frøhlich
Nature goes full open review!
I have complicated feelings about how and when this was achieved!
And they should be apparent to you after even a moment’s thought about the subtext here!
www.nature.com/articles/d41...
I have complicated feelings about how and when this was achieved!
And they should be apparent to you after even a moment’s thought about the subtext here!
www.nature.com/articles/d41...
Transparent peer review to be extended to all of Nature’s research papers
From today, all new submissions to Nature that are published will be accompanied by referees’ reports and author responses — to illuminate the process of producing rigorous science.
www.nature.com
June 17, 2025 at 12:21 PM
Nature goes full open review!
I have complicated feelings about how and when this was achieved!
And they should be apparent to you after even a moment’s thought about the subtext here!
www.nature.com/articles/d41...
I have complicated feelings about how and when this was achieved!
And they should be apparent to you after even a moment’s thought about the subtext here!
www.nature.com/articles/d41...
Reposted by Christopher Frøhlich
I highly recommend this conference, i thoroughly enjoyed the 2022 meeting 👇🏻
The abstract submission deadline for Understanding and predicting microbial evolutionary dynamics 2025 is soon approaching! Make sure to submit by 23:59 BST on 7 July 2025. microb.io/Abstracts #MicroEvo25
June 18, 2025 at 5:56 PM
I highly recommend this conference, i thoroughly enjoyed the 2022 meeting 👇🏻
Reposted by Christopher Frøhlich
#JournalClub
🚨 Out Now!
A short read describing a xanthone-derived antibiotic which has multifaceted mode of action.
#MicroSky #AMR
www.nature.com/articles/s41...
🚨 Out Now!
A short read describing a xanthone-derived antibiotic which has multifaceted mode of action.
#MicroSky #AMR
www.nature.com/articles/s41...
A xanthone-derived antibiotic with a multifaceted mode of action - Nature Microbiology
Nature Microbiology - A xanthone-derived antibiotic with a multifaceted mode of action
www.nature.com
June 18, 2025 at 1:47 PM
#JournalClub
🚨 Out Now!
A short read describing a xanthone-derived antibiotic which has multifaceted mode of action.
#MicroSky #AMR
www.nature.com/articles/s41...
🚨 Out Now!
A short read describing a xanthone-derived antibiotic which has multifaceted mode of action.
#MicroSky #AMR
www.nature.com/articles/s41...
Reposted by Christopher Frøhlich
🔬 Join us! We are hiring three PhD students to advance enzyme design and develop photoenzymes for carbon capture and nitrogen fixation. If you are passionate about protein engineering, computational modeling, or synthetic biology, check out the job openings!
📌 www.mpg.de/24336572/phd...
📌 www.mpg.de/24336572/phd...
Three PhD positions | Computational protein design
Three PhD postiions in computational protein design
www.mpg.de
March 16, 2025 at 7:39 AM
🔬 Join us! We are hiring three PhD students to advance enzyme design and develop photoenzymes for carbon capture and nitrogen fixation. If you are passionate about protein engineering, computational modeling, or synthetic biology, check out the job openings!
📌 www.mpg.de/24336572/phd...
📌 www.mpg.de/24336572/phd...
🔬 PhD in Antibiotic Resistance Evolution – Join Us in the Arctic! ❄️
Passionate about enzymes and tackling antibiotic resistance?🧬✨
🔍 We’re looking for candidates with experience in:
✅ Genetic manipulation
✅ Protein expression & purification
✅ Enzyme biochemistry
🔗 Apply now: lnkd.in/eZRrgJKF
Passionate about enzymes and tackling antibiotic resistance?🧬✨
🔍 We’re looking for candidates with experience in:
✅ Genetic manipulation
✅ Protein expression & purification
✅ Enzyme biochemistry
🔗 Apply now: lnkd.in/eZRrgJKF
LinkedIn
This link will take you to a page that’s not on LinkedIn
lnkd.in
February 19, 2025 at 3:44 PM
🔬 PhD in Antibiotic Resistance Evolution – Join Us in the Arctic! ❄️
Passionate about enzymes and tackling antibiotic resistance?🧬✨
🔍 We’re looking for candidates with experience in:
✅ Genetic manipulation
✅ Protein expression & purification
✅ Enzyme biochemistry
🔗 Apply now: lnkd.in/eZRrgJKF
Passionate about enzymes and tackling antibiotic resistance?🧬✨
🔍 We’re looking for candidates with experience in:
✅ Genetic manipulation
✅ Protein expression & purification
✅ Enzyme biochemistry
🔗 Apply now: lnkd.in/eZRrgJKF
📣 Preprint Alert: How can evolution recruit existing enzymes during bacterial lifestyle changes?
Discover how mutations in a bifunctional enzyme 🔄 alter bacterial c-di-GMP metabolism, decoupling it from external polyamine signaling, and steering cells from a planktonic to a biofilm lifestyle 🦠
Discover how mutations in a bifunctional enzyme 🔄 alter bacterial c-di-GMP metabolism, decoupling it from external polyamine signaling, and steering cells from a planktonic to a biofilm lifestyle 🦠
Biofilm selection constitutively activates c-di-GMP synthesis by the bifunctional enzyme MbaA
Biofilms, in which microbes are encased within a self-produced matrix, represent the primary mode of microbial life. Yet, our understanding of the molecular mechanisms governing biofilm formation rema...
www.biorxiv.org
February 14, 2025 at 8:38 AM
📣 Preprint Alert: How can evolution recruit existing enzymes during bacterial lifestyle changes?
Discover how mutations in a bifunctional enzyme 🔄 alter bacterial c-di-GMP metabolism, decoupling it from external polyamine signaling, and steering cells from a planktonic to a biofilm lifestyle 🦠
Discover how mutations in a bifunctional enzyme 🔄 alter bacterial c-di-GMP metabolism, decoupling it from external polyamine signaling, and steering cells from a planktonic to a biofilm lifestyle 🦠
🌟 Enzyme Evolution: Marie Curie Fellowship in Tromsø! 🌟
Are you a late-stage PhD student or postdoctoral scholar specializing in biochemistry, molecular biology, or a related discipline? Connect with us to explore the potential for a MSCA collaboration.
euraxess.ec.europa.eu/jobs/309710
Are you a late-stage PhD student or postdoctoral scholar specializing in biochemistry, molecular biology, or a related discipline? Connect with us to explore the potential for a MSCA collaboration.
euraxess.ec.europa.eu/jobs/309710
Call for postdoc candidates interested in applying for an MSCA Postdoctoral Fellowship in Enzyme Evolution
UiT The Arctic University of Norway has established «The Arctic MSCA-PF program» (uit.no/project/arcticmsca) to recruit excellent young researchers planning to apply for a Marie Skƚodowsk
euraxess.ec.europa.eu
January 20, 2025 at 1:32 PM
🌟 Enzyme Evolution: Marie Curie Fellowship in Tromsø! 🌟
Are you a late-stage PhD student or postdoctoral scholar specializing in biochemistry, molecular biology, or a related discipline? Connect with us to explore the potential for a MSCA collaboration.
euraxess.ec.europa.eu/jobs/309710
Are you a late-stage PhD student or postdoctoral scholar specializing in biochemistry, molecular biology, or a related discipline? Connect with us to explore the potential for a MSCA collaboration.
euraxess.ec.europa.eu/jobs/309710
Are you interested in biofilms and how evolution regulates them?
Follow @oeyvindml.bsky.social PhD defence online. 1045 Central European time!
Here is the link to the live stream: uit.cloud.panopto.eu/Panopto/Page...
Follow @oeyvindml.bsky.social PhD defence online. 1045 Central European time!
Here is the link to the live stream: uit.cloud.panopto.eu/Panopto/Page...
December 5, 2024 at 6:40 PM
Are you interested in biofilms and how evolution regulates them?
Follow @oeyvindml.bsky.social PhD defence online. 1045 Central European time!
Here is the link to the live stream: uit.cloud.panopto.eu/Panopto/Page...
Follow @oeyvindml.bsky.social PhD defence online. 1045 Central European time!
Here is the link to the live stream: uit.cloud.panopto.eu/Panopto/Page...
Reposted by Christopher Frøhlich
Are you interested in how #epistasis shapes the AMR evolution?
We showed that epistasis can be caused by independently modulating substrate binding and the chemical step ultimately changing the rate-limiting step in the catalytic cycle. @NatureCatalysis
Full read: rdcu.be/dzqjk
We showed that epistasis can be caused by independently modulating substrate binding and the chemical step ultimately changing the rate-limiting step in the catalytic cycle. @NatureCatalysis
Full read: rdcu.be/dzqjk
Epistasis arises from shifting the rate-limiting step during enzyme evolution of a β-lactamase
Nature Catalysis - The reasons for epistasis, wherein mutations interact non-additively, are often not fully understood. Now it is found that shifting the rate-limiting step from substrate binding...
rdcu.be
February 23, 2024 at 11:09 AM
Are you interested in how #epistasis shapes the AMR evolution?
We showed that epistasis can be caused by independently modulating substrate binding and the chemical step ultimately changing the rate-limiting step in the catalytic cycle. @NatureCatalysis
Full read: rdcu.be/dzqjk
We showed that epistasis can be caused by independently modulating substrate binding and the chemical step ultimately changing the rate-limiting step in the catalytic cycle. @NatureCatalysis
Full read: rdcu.be/dzqjk
Are you interested in how #epistasis shapes the AMR evolution?
We showed that epistasis can be caused by independently modulating substrate binding and the chemical step ultimately changing the rate-limiting step in the catalytic cycle. @NatureCatalysis
Full read: rdcu.be/dzqjk
We showed that epistasis can be caused by independently modulating substrate binding and the chemical step ultimately changing the rate-limiting step in the catalytic cycle. @NatureCatalysis
Full read: rdcu.be/dzqjk
Epistasis arises from shifting the rate-limiting step during enzyme evolution of a β-lactamase
Nature Catalysis - The reasons for epistasis, wherein mutations interact non-additively, are often not fully understood. Now it is found that shifting the rate-limiting step from substrate binding...
rdcu.be
February 23, 2024 at 11:09 AM
Are you interested in how #epistasis shapes the AMR evolution?
We showed that epistasis can be caused by independently modulating substrate binding and the chemical step ultimately changing the rate-limiting step in the catalytic cycle. @NatureCatalysis
Full read: rdcu.be/dzqjk
We showed that epistasis can be caused by independently modulating substrate binding and the chemical step ultimately changing the rate-limiting step in the catalytic cycle. @NatureCatalysis
Full read: rdcu.be/dzqjk
Postdoc Opportunity UiTNorgesarktis! 🇳🇴
🧪Join our Arctic MSCA-PF program in Enzyme & AMR evolution. 24-months MSCA in Tromsø's stunning landscape.
⏱️Apply by Feb 16: 3-page CV & 2-page proposal to cfr016@uit.no.
#sciencejobs #postdocposition
euraxess.ec.europa.eu/jobs/189491
🧪Join our Arctic MSCA-PF program in Enzyme & AMR evolution. 24-months MSCA in Tromsø's stunning landscape.
⏱️Apply by Feb 16: 3-page CV & 2-page proposal to cfr016@uit.no.
#sciencejobs #postdocposition
euraxess.ec.europa.eu/jobs/189491
Call for postdoc candidates interested in applying for a MSCA Postdoctoral Fellowship in Enzyme and ...
UiT The Arctic University of Norway has established «The Arctic MSCA-PF program» (uit.no/project/arcticmsca) to recruit excellent young researchers planning to apply for a Marie Skƚodowska-Curie Po...
euraxess.ec.europa.eu
January 26, 2024 at 10:01 AM
Postdoc Opportunity UiTNorgesarktis! 🇳🇴
🧪Join our Arctic MSCA-PF program in Enzyme & AMR evolution. 24-months MSCA in Tromsø's stunning landscape.
⏱️Apply by Feb 16: 3-page CV & 2-page proposal to cfr016@uit.no.
#sciencejobs #postdocposition
euraxess.ec.europa.eu/jobs/189491
🧪Join our Arctic MSCA-PF program in Enzyme & AMR evolution. 24-months MSCA in Tromsø's stunning landscape.
⏱️Apply by Feb 16: 3-page CV & 2-page proposal to cfr016@uit.no.
#sciencejobs #postdocposition
euraxess.ec.europa.eu/jobs/189491
🌟Exciting News! Our AMR & Biofilms research just received an 8M kroner grant from #HelseNord. Big thanks for supporting young researchers and fueling scientific innovation. Grateful to all who've contributed to this success. Looking forward to what comes next.
December 26, 2023 at 12:44 PM
🌟Exciting News! Our AMR & Biofilms research just received an 8M kroner grant from #HelseNord. Big thanks for supporting young researchers and fueling scientific innovation. Grateful to all who've contributed to this success. Looking forward to what comes next.