Brian Mueller
@brianscience.bsky.social
Visiting Postdoc @Charite @MPI-NAT| PhD @UUtah | CUBoulder ‘13. Calcium channels, release machinery, and synaptic function. (he/him)
Reposted by Brian Mueller
New preprint!
It turns out you can integrate arrays with super high efficiency using PhiC31.
www.biorxiv.org/content/10.1...
It turns out you can integrate arrays with super high efficiency using PhiC31.
www.biorxiv.org/content/10.1...
High-efficiency targeted integration of extrachromosomal arrays in C. elegans using PhiC31 integrase
Extrachromosomal arrays are unique chromosome-like structures created from DNA injected into the C. elegans germline. Arrays are easy to create and allow for high expression of multiple transgenes. Th...
www.biorxiv.org
November 13, 2025 at 2:59 AM
New preprint!
It turns out you can integrate arrays with super high efficiency using PhiC31.
www.biorxiv.org/content/10.1...
It turns out you can integrate arrays with super high efficiency using PhiC31.
www.biorxiv.org/content/10.1...
Looking for recommendations for a AAV purification and concentration kit that does not require ultracentrifugation.
<3
<3
July 11, 2025 at 7:48 PM
Looking for recommendations for a AAV purification and concentration kit that does not require ultracentrifugation.
<3
<3
hands off our science pls
June 9, 2025 at 5:49 PM
hands off our science pls
Reposted by Brian Mueller
🚨I converted the list of terminated #NIH grants (as of 3/22) into a google sheet. I added activity codes and award jurisdictions (see tabs for grant count and $ by each). Note this HHS list likely does not include all planned/completed terminations as of this date.🚨 Please use, share and distribute!
March 23, 2025 at 1:47 AM
🚨I converted the list of terminated #NIH grants (as of 3/22) into a google sheet. I added activity codes and award jurisdictions (see tabs for grant count and $ by each). Note this HHS list likely does not include all planned/completed terminations as of this date.🚨 Please use, share and distribute!
Reposted by Brian Mueller
These Senators are on the fence about voting NO on the CR:
Elissa Slotkin (MI)
Gary Peters (MI)
Jacky Rosen (NV)
Jeanne Shaheen (NH)
Maggie Hassan (NH)
Jon Ossoff (GA)
John Hickenlooper (CO)
Michael Bennett (CO)
Mark Kelly (AZ)
Peter Welch (VT)
Mark Warner (VA)
Time Kaine (VA)
Call (202) 224-3121
Elissa Slotkin (MI)
Gary Peters (MI)
Jacky Rosen (NV)
Jeanne Shaheen (NH)
Maggie Hassan (NH)
Jon Ossoff (GA)
John Hickenlooper (CO)
Michael Bennett (CO)
Mark Kelly (AZ)
Peter Welch (VT)
Mark Warner (VA)
Time Kaine (VA)
Call (202) 224-3121
March 12, 2025 at 4:39 PM
These Senators are on the fence about voting NO on the CR:
Elissa Slotkin (MI)
Gary Peters (MI)
Jacky Rosen (NV)
Jeanne Shaheen (NH)
Maggie Hassan (NH)
Jon Ossoff (GA)
John Hickenlooper (CO)
Michael Bennett (CO)
Mark Kelly (AZ)
Peter Welch (VT)
Mark Warner (VA)
Time Kaine (VA)
Call (202) 224-3121
Elissa Slotkin (MI)
Gary Peters (MI)
Jacky Rosen (NV)
Jeanne Shaheen (NH)
Maggie Hassan (NH)
Jon Ossoff (GA)
John Hickenlooper (CO)
Michael Bennett (CO)
Mark Kelly (AZ)
Peter Welch (VT)
Mark Warner (VA)
Time Kaine (VA)
Call (202) 224-3121
Reposted by Brian Mueller
(To the tune of "Eleanor Rigby")
🎵
Barbara McClintock
Spends all her days counting genes in kernels of corncobs
(It's all transposons)
...
All these purple kernels
Where do they all come from?
🎵
🎵
Barbara McClintock
Spends all her days counting genes in kernels of corncobs
(It's all transposons)
...
All these purple kernels
Where do they all come from?
🎵
February 21, 2025 at 8:24 PM
(To the tune of "Eleanor Rigby")
🎵
Barbara McClintock
Spends all her days counting genes in kernels of corncobs
(It's all transposons)
...
All these purple kernels
Where do they all come from?
🎵
🎵
Barbara McClintock
Spends all her days counting genes in kernels of corncobs
(It's all transposons)
...
All these purple kernels
Where do they all come from?
🎵
Disappointed that GLP-1 does not stand for 'Gila Lizard Protein 1'.
February 10, 2025 at 10:29 AM
Disappointed that GLP-1 does not stand for 'Gila Lizard Protein 1'.
Berlin Wall 20250208
February 8, 2025 at 7:04 PM
Berlin Wall 20250208
Reposted by Brian Mueller
Reposted by Brian Mueller
Earth Explodes
theonion.com/earth-e...
theonion.com/earth-e...
January 20, 2025 at 11:00 PM
Earth Explodes
theonion.com/earth-e...
theonion.com/earth-e...
Late submission to fluorescence Friday. But here's a synapse that's a little cattywampus and colored to look like the UA flag.
January 4, 2025 at 5:55 PM
Late submission to fluorescence Friday. But here's a synapse that's a little cattywampus and colored to look like the UA flag.
2025 resolution. do a biochemistry.
December 31, 2024 at 9:28 PM
2025 resolution. do a biochemistry.
If you look up ‘milquetoast’ in the dictionary there’s just a picture of Merrick Garland.
November 25, 2024 at 11:32 PM
If you look up ‘milquetoast’ in the dictionary there’s just a picture of Merrick Garland.
Post a non-religious photo you think of as holy.
November 24, 2024 at 11:30 AM
Post a non-religious photo you think of as holy.
NYTimes et al. be like: TRUMP DENIES PROJECT 2025 TIES (but he's - probably - maybe - not being completely honest. How are we to know for sure?). The telegraphed despotism needed to be on the front page daily.
November 23, 2024 at 8:51 AM
NYTimes et al. be like: TRUMP DENIES PROJECT 2025 TIES (but he's - probably - maybe - not being completely honest. How are we to know for sure?). The telegraphed despotism needed to be on the front page daily.
First of many #FluorescenceFriday on Bluesky.
3D-superresolution image of cultured cortical neurons with active zones (cyan) marked along with various ion channels (yellow, magenta). 50 um field of view. 20 nm resolution. Vutara VXL @brukercorporation.bsky.social
3D-superresolution image of cultured cortical neurons with active zones (cyan) marked along with various ion channels (yellow, magenta). 50 um field of view. 20 nm resolution. Vutara VXL @brukercorporation.bsky.social
November 22, 2024 at 2:18 PM
First of many #FluorescenceFriday on Bluesky.
3D-superresolution image of cultured cortical neurons with active zones (cyan) marked along with various ion channels (yellow, magenta). 50 um field of view. 20 nm resolution. Vutara VXL @brukercorporation.bsky.social
3D-superresolution image of cultured cortical neurons with active zones (cyan) marked along with various ion channels (yellow, magenta). 50 um field of view. 20 nm resolution. Vutara VXL @brukercorporation.bsky.social
Reposted by Brian Mueller
Research funded by NIH generated $2.46 in economic activity for every $1 of funding in 2023 [a total of $92.9 billion]
www.fiercebiotech.com/research/rep...
www.fiercebiotech.com/research/rep...
Report: Every dollar of NIH research funding doubles in economic returns
Research funded by the National Institutes of Health (NIH) generated $2.46 in economic activity for every $1 of funding in 2023, a total of $92.9 billion, according to an
www.fiercebiotech.com
November 17, 2024 at 11:56 PM
Research funded by NIH generated $2.46 in economic activity for every $1 of funding in 2023 [a total of $92.9 billion]
www.fiercebiotech.com/research/rep...
www.fiercebiotech.com/research/rep...
First Bassoon images off the scope. I think it looks right?
Magenta - WF
Green - 3D-SMLM
Magenta - WF
Green - 3D-SMLM
November 20, 2024 at 9:03 AM
First Bassoon images off the scope. I think it looks right?
Magenta - WF
Green - 3D-SMLM
Magenta - WF
Green - 3D-SMLM
On the bright side, only 41 more episodes of Great British Bake Off until Trump is out of office.
November 16, 2024 at 8:50 PM
On the bright side, only 41 more episodes of Great British Bake Off until Trump is out of office.
Nothing compares to being in a room full of scientists, gathered around a microscope, getting excited about what they are seeing 😊
November 15, 2024 at 7:50 PM
Nothing compares to being in a room full of scientists, gathered around a microscope, getting excited about what they are seeing 😊
Goodbye hell site 🦋
November 13, 2024 at 4:38 PM
Goodbye hell site 🦋